Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,246,015	T→C	A454A (GCA→GCG)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,246,015	0	T	C	100.0%	274.5 / NA	76	A454A (GCA→GCG)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/35);  total (41/35)

GCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGA  >  NC_000913/1245874‑1246157                                                                                                                                              |                                                                                                                                               gCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCa                                                                                                                                       <  2:1214216/151‑1 (MQ=255) gCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCa                                                                                                                                       <  2:1232808/151‑1 (MQ=255)  cTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCAt                                                                                                                                      >  2:308452/1‑151 (MQ=255)      catcatATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTgg                                                                                                                                  >  2:272695/1‑151 (MQ=255)      catcatATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTgg                                                                                                                                  <  1:858833/151‑1 (MQ=255)        tcatATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGgcg                                                                                                                                >  2:34667/1‑151 (MQ=255)         catATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGgcgc                                                                                                                               >  2:35992/1‑151 (MQ=255)          atatTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGgcgc                                                                                                                               >  1:1149345/1‑150 (MQ=255)           tatTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCAt                                                                                                                             >  1:324021/1‑151 (MQ=255)            atTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATc                                                                                                                            >  2:256177/1‑151 (MQ=255)              ttttCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATccc                                                                                                                          >  1:1235421/1‑151 (MQ=255)                ttCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCAt                                                                                                                        >  2:110310/1‑151 (MQ=255)                 tCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCAt                                                                                                                        <  2:979434/150‑1 (MQ=255)                  ccaccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCAtt                                                                                                                       <  1:193957/150‑1 (MQ=255)                             ttCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACttt                                                                                                           >  2:1267844/1‑151 (MQ=255)                              tctcCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACtttt                                                                                                          >  1:336755/1‑151 (MQ=255)                               ctcCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTc                                                                                                         >  2:538779/1‑151 (MQ=255)                               ctcCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTc                                                                                                         >  2:703353/1‑151 (MQ=255)                                  ccGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCAcc                                                                                                      >  2:237185/1‑151 (MQ=255)                                      tCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACg                                                                                                  >  1:1103475/1‑151 (MQ=255)                                       cATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACgt                                                                                                 <  1:881236/151‑1 (MQ=255)                                              gtgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGtt                                                                                           <  1:543533/150‑1 (MQ=255)                                               tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTc                                                                                          >  1:794752/1‑150 (MQ=255)                                                   gAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgcc                                                                                     <  1:298894/151‑1 (MQ=255)                                                   gAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgcc                                                                                     <  2:462771/151‑1 (MQ=255)                                                       aTTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGccgccg                                                                                 <  1:421537/151‑1 (MQ=255)                                                        ttGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCgg                                                                                >  1:573612/1‑151 (MQ=255)                                                          ggTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGt                                                                              <  2:345380/151‑1 (MQ=255)                                                          ggTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGt                                                                              >  1:990549/1‑151 (MQ=255)                                                           gTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGtt                                                                             <  1:197358/151‑1 (MQ=255)                                                             cAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTgc                                                                           >  2:897774/1‑151 (MQ=255)                                                             cAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTgc                                                                           >  2:518250/1‑151 (MQ=255)                                                             cAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTg                                                                            <  1:1241438/150‑1 (MQ=255)                                                                 aaGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTgcagca                                                                       <  1:640003/151‑1 (MQ=255)                                                                     gCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGAtg                                                                   <  1:211317/151‑1 (MQ=255)                                                                     gCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGAt                                                                    <  2:1112759/150‑1 (MQ=255)                                                                     gCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGAt                                                                    <  1:802967/150‑1 (MQ=255)                                                                           tAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGtttt                                                             >  1:827241/1‑151 (MQ=255)                                                                            aaTGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTtc                                                            <  2:843669/151‑1 (MQ=255)                                                                                tCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCATTTTTCACCGCCGTGGTGTTCAGGCCGCCGGTTTGCAGCAGATGTGTTTtcgtc                                                         <  1:817668/150‑1 (MQ=255)                                                                                 ccATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgc                                                       <  1:825869/151‑1 (MQ=255)                                                                                  cATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgcc                                                      >  1:810507/1‑151 (MQ=255)                                                                                   aTCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccg                                                     >  2:762772/1‑151 (MQ=255)                                                                                    tCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgt                                                    >  2:423954/1‑151 (MQ=255)                                                                                    ccGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTCACGCCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGCGGCGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccg                                                     >  1:201245/2‑150 (MQ=255)                                                                                     cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgt                                                    <  1:640813/150‑1 (MQ=255)                                                                                       ccACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtcgc                                                 >  2:6713/1‑151 (MQ=255)                                                                                        cACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCgtcgccgtcgcc                                                >  2:998099/1‑151 (MQ=255)                                                                                            tctTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAt                                              <  2:1274160/149‑1 (MQ=255)                                                                                             ctTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtttt                                           >  2:1288088/1‑151 (MQ=255)                                                                                              tttttGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                          >  2:941715/1‑151 (MQ=255)                                                                                               ttttGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                         >  1:698051/1‑151 (MQ=255)                                                                                                 ttGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgtt                                        >  1:479894/1‑150 (MQ=255)                                                                                                    cccGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgc                                     <  1:1004057/150‑1 (MQ=255)                                                                                                     ccGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcgc                                   >  2:880995/1‑151 (MQ=255)                                                                                                       gTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGcgcg                                  >  1:43682/1‑150 (MQ=255)                                                                                                         aGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATc                               <  2:118360/151‑1 (MQ=255)                                                                                                         aGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGACGCCGTCGCCATTTTGTTGGCGCGATc                               >  1:337061/1‑151 (MQ=255)                                                                                                          gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTG‑TGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCtt                             >  1:25118/1‑151 (MQ=255)                                                                                                                 aaTCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTg                       >  2:226801/1‑151 (MQ=255)                                                                                                                 aaTCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGACGCATCCCATTGTTGCCCACTTTTCACCGCCGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCt                        >  2:980266/1‑150 (MQ=255)                                                                                                                    ccTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGcc                     <  2:154611/150‑1 (MQ=255)                                                                                                                    ccTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCg                    >  2:392518/1‑151 (MQ=255)                                                                                                                       tCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCa                  <  1:662184/150‑1 (MQ=255)                                                                                                                          gTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                <  2:1149379/149‑1 (MQ=255)                                                                                                                          gTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTg               <  1:495057/150‑1 (MQ=255)                                                                                                                          gTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa              <  2:890466/151‑1 (MQ=255)                                                                                                                            cgcgACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGAc             <  2:306937/150‑1 (MQ=255)                                                                                                                             gcgACCCACTGTAACGGCGCCCCGCCATTTGTCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGGGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAGTGa              <  1:1274880/148‑1 (MQ=255)                                                                                                                               gACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTAc         >  2:222709/1‑151 (MQ=255)                                                                                                                               gACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGGGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGAGCGATCTTTCGCTGCCGCATTGACGTAc         >  1:982904/1‑151 (MQ=255)                                                                                                                                aCCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACa        <  2:855179/151‑1 (MQ=255)                                                                                                                                aCCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACa        <  1:470281/151‑1 (MQ=255)                                                                                                                                aCCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACa        <  1:176057/151‑1 (MQ=255)                                                                                                                                     cTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCggg   >  2:1026244/1‑151 (MQ=255)                                                                                                                                       gTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGa  <  2:189307/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                               GCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGA  >  NC_000913/1245874‑1246157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑