Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	218,942	A→G	V218A (GTT→GCT)	tsaA ←	tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	218,942	0	A	G	100.0%	317.4 / NA	88	V218A (GTT→GCT)	tsaA	tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/46);  total (42/46)

GGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGA  >  NC_000913/218794‑219089                                                                                                                                                     |                                                                                                                                                    ggAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCagcgagcg                                                                                                                                                   >  2:916077/1‑151 (MQ=255) ggAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCagcgagcg                                                                                                                                                   >  2:566257/1‑151 (MQ=255)    aGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGtt                                                                                                                                                 >  2:835770/1‑150 (MQ=255)        aGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTaaaa                                                                                                                                             <  2:1204585/150‑1 (MQ=255)        aGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAAt                                                                                                                                            >  2:939726/1‑151 (MQ=255)            cAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATg                                                                                                                                        >  2:1124551/1‑151 (MQ=255)                      gCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGAct                                                                                                                              <  1:1187040/151‑2 (MQ=255)                      gCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACg                                                                                                                              <  1:738143/151‑1 (MQ=255)                      gCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACg                                                                                                                              <  1:1198229/151‑1 (MQ=255)                      gCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACg                                                                                                                              <  1:821282/151‑1 (MQ=255)                        gAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGc                                                                                                                            >  1:819118/1‑151 (MQ=255)                        gAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGc                                                                                                                            <  2:170015/151‑1 (MQ=255)                         aGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCa                                                                                                                           <  2:557790/151‑1 (MQ=255)                          gCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCa                                                                                                                           <  2:828348/150‑1 (MQ=255)                            cTGGGACAAAAAAAAGTGATTTAGCTTACAAGAGCAAAAGAAATGTCAAAAGAGAAGGGCGCGAATTTAACGCGGTTCCAGCGAAAAGACTACAAAACCTGCGTCGGTGAAGCGCCAGCGAGCGTTAAAATCATGTAAACAGACGGCa                                                                                                                           >  1:1065937/1‑148 (MQ=255)                              gggACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAgg                                                                                                                       <  1:1225559/150‑1 (MQ=255)                              gggACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGt                                                                                                                      <  2:1118088/151‑1 (MQ=255)                                gACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGttt                                                                                                                    >  1:368688/1‑151 (MQ=255)                                gACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGttt                                                                                                                    <  1:634855/151‑1 (MQ=255)                                 aCAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGtttt                                                                                                                   <  2:592547/151‑1 (MQ=255)                                   aaaaaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTg                                                                                                                  >  1:843194/1‑150 (MQ=255)                                   aaaaaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGc                                                                                                                 >  2:498567/1‑151 (MQ=255)                                      aaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCg                                                                                                              >  2:971960/1‑151 (MQ=255)                                      aaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCg                                                                                                              >  1:646333/1‑151 (MQ=255)                                       aaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGt                                                                                                             <  2:159225/151‑1 (MQ=255)                                        aaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGtt                                                                                                            >  1:808039/1‑151 (MQ=255)                                         aaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGttt                                                                                                           <  2:1271093/151‑1 (MQ=255)                                          aGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTc                                                                                                          <  2:1179325/151‑1 (MQ=255)                                          aGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTc                                                                                                          <  2:979883/151‑1 (MQ=255)                                             gATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc                                                                                                       >  1:229877/1‑151 (MQ=255)                                             gATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc                                                                                                       >  2:831049/1‑151 (MQ=255)                                              aTTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCa                                                                                                      <  2:837797/151‑1 (MQ=255)                                                   gTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCttt                                                                                                 >  2:243097/1‑151 (MQ=255)                                                    tttACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCttt                                                                                                 >  1:555107/1‑150 (MQ=255)                                                     ttACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc                                                                                               >  1:28669/1‑151 (MQ=255)                                                      tACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc                                                                                               <  2:65731/150‑1 (MQ=255)                                                       aCCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACg                                                                                              <  1:158721/150‑1 (MQ=255)                                                       aCCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGa                                                                                             <  1:939687/151‑1 (MQ=255)                                                        ccAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGAt                                                                                            >  2:1128593/1‑151 (MQ=255)                                                         cAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGAt                                                                                            >  1:658792/1‑150 (MQ=255)                                                         cAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGAt                                                                                            >  2:1280808/1‑150 (MQ=255)                                                         cAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATa                                                                                           <  1:381939/151‑1 (MQ=255)                                                          aGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGAt                                                                                            >  1:33847/1‑149 (MQ=255)                                                          aGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATa                                                                                           <  1:409235/150‑1 (MQ=255)                                                           gTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAgg                                                                                         <  1:599249/151‑1 (MQ=255)                                                             gCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGcc                                                                                       <  2:1285822/151‑1 (MQ=255)                                                              cAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCg                                                                                      >  2:410550/1‑151 (MQ=255)                                                               aaaaGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCgg                                                                                     >  1:26424/1‑151 (MQ=255)                                                                aaaGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCggg                                                                                    >  1:1184548/1‑151 (MQ=255)                                                                 aaGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGgc                                                                                   >  2:759698/1‑151 (MQ=255)                                                                   gAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTT‑CAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACCGCATAGGTTTTGCCCGTTTCCTCACCTTTACCATAAGCCGGgcgc                                                                                 >  1:518584/1‑150 (MQ=255)                                                                    aaaTGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg                                                                                >  1:1222407/1‑151 (MQ=255)                                                                     aaTGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCgg                                                                               <  2:705973/151‑1 (MQ=255)                                                                       tGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGCGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGt                                                                             >  1:115983/1‑151 (MQ=255)                                                                        gTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTc                                                                            <  1:905283/151‑1 (MQ=255)                                                                         tCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTcc                                                                           >  2:412691/1‑151 (MQ=255)                                                                           aaaaGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTg                                                                         >  1:749660/1‑151 (MQ=255)                                                                             aaGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTgcg                                                                       <  2:928314/151‑1 (MQ=255)                                                                               gagaAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGcc                                                                     >  2:652927/1‑151 (MQ=255)                                                                                      gCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACtt                                                              <  1:567016/151‑1 (MQ=255)                                                                                        gTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTcg                                                            <  1:1141723/151‑1 (MQ=255)                                                                                           aaTTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCgg                                                         >  2:1157800/1‑151 (MQ=255)                                                                                           aaTTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCgg                                                         >  2:1122452/1‑151 (MQ=255)                                                                                           aaTTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCgg                                                         <  1:1280767/151‑1 (MQ=255)                                                                                           aaTTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCgg                                                         >  2:291586/1‑151 (MQ=255)                                                                                             tttAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGa                                                        >  1:1008013/1‑150 (MQ=255)                                                                                              ttAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGAt                                                       >  2:875763/1‑150 (MQ=255)                                                                                               tAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGTTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGAt                                                       <  2:694919/149‑1 (MQ=255)                                                                                                     ggTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGCCGGCATAGGTTTTGCCCGTTTCCTCACCGTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGc                                                <  1:812211/150‑1 (MQ=255)                                                                                                      gTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGt                                              <  2:496841/151‑1 (MQ=255)                                                                                                        tCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGtt                                             <  2:1073041/150‑1 (MQ=255)                                                                                                        tCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGtt                                             >  1:796326/1‑150 (MQ=255)                                                                                                          cAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAAc                                          <  2:911665/151‑1 (MQ=255)                                                                                                           aGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACt                                         <  1:1077102/151‑1 (MQ=255)                                                                                                                aaaGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGa                                    <  1:166083/151‑1 (MQ=255)                                                                                                                   gACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCTTAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATa                                  <  1:566975/150‑1 (MQ=255)                                                                                                                    aCTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAAc                                >  2:552551/1‑151 (MQ=255)                                                                                                                        cAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACg                               <  1:914494/148‑1 (MQ=255)                                                                                                                          aaaCCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTtct                          <  2:775977/151‑1 (MQ=255)                                                                                                                           aaCCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTtctc                         >  1:219400/1‑151 (MQ=255)                                                                                                                                gCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCg                    <  2:1186245/151‑1 (MQ=255)                                                                                                                                gCGTCGGAGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAAGATAGGCCGGGCGCGGGACATGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCg                    >  1:968230/1‑151 (MQ=255)                                                                                                                                 cGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGt                   >  1:943696/1‑151 (MQ=255)                                                                                                                                     ggTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCaaa               <  2:133534/151‑1 (MQ=255)                                                                                                                                     ggTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCaa                >  2:139087/1‑150 (MQ=255)                                                                                                                                           gcgcCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgctgc         >  2:44463/1‑151 (MQ=255)                                                                                                                                            cgcCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgctgct        <  2:311958/151‑1 (MQ=255)                                                                                                                                               cAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtttt     <  1:842231/151‑1 (MQ=255)                                                                                                                                               cAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtttt     <  1:1222217/151‑1 (MQ=255)                                                                                                                                                agcgagcgTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTc    <  1:1196065/151‑1 (MQ=255)                                                                                                                                                agcgagcgTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTc    >  2:483718/1‑151 (MQ=255)                                                                                                                                                  cGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGa  <  2:1078567/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGA  >  NC_000913/218794‑219089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑