Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	219,435	A→G	F54L (TTC→CTC)	tsaA ←	tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	219,435	0	A	G	100.0%	316.1 / NA	83	F54L (TTC→CTC)	tsaA	tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/42);  total (41/42)

ATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAA  >  NC_000913/219290‑219579                                                                                                                                                  |                                                                                                                                                 aTGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCtt                                                                                                                                             <  2:517218/151‑1 (MQ=255)  tGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCtt                                                                                                                                             <  1:1148366/150‑1 (MQ=255)    ccAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCa                                                                                                                                          <  1:795995/151‑1 (MQ=255)      aaTTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAgg                                                                                                                                        >  1:452247/1‑151 (MQ=255)        ttGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGcc                                                                                                                                      >  1:342820/1‑151 (MQ=255)         tGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGcc                                                                                                                                      <  2:520127/150‑1 (MQ=255)          gggTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCcgc                                                                                                                                    >  2:871374/1‑151 (MQ=255)          gggTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCcg                                                                                                                                     <  1:1040243/150‑1 (MQ=255)             ttAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGa                                                                                                                                  >  2:536193/1‑150 (MQ=255)              tAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGaa                                                                                                                                 <  2:19197/150‑1 (MQ=255)               aGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACg                                                                                                                               >  1:281719/1‑151 (MQ=255)                    ggAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTc                                                                                                                          >  2:590181/1‑151 (MQ=255)                     gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCg                                                                                                                         >  1:947307/1‑151 (MQ=255)                       aagaagagagaggggCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGAAGCCAGCCGAATTGCAAAGGTAGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGc                                                                                                                       >  1:866634/15‑151 (MQ=255)                        aGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGcc                                                                                                                      >  2:445098/1‑151 (MQ=255)                              gCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGtt                                                                                                                 <  2:115975/150‑1 (MQ=255)                                       gAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCa                                                                                                       <  1:594987/151‑1 (MQ=255)                                       gAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCa                                                                                                       <  1:35052/151‑1 (MQ=255)                                        aaaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCaa                                                                                                      <  1:812045/151‑1 (MQ=255)                                         aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAAt                                                                                                     >  1:1087316/1‑151 (MQ=255)                                          aaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATg                                                                                                    <  2:1282684/151‑1 (MQ=255)                                          aaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATg                                                                                                    >  1:917566/1‑151 (MQ=255)                                            cccccATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATgag                                                                                                  <  1:1108839/151‑1 (MQ=255)                                               ccATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATGAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATg                                                                                               <  2:1224964/151‑1 (MQ=255)                                               ccATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATg                                                                                               >  2:862255/1‑151 (MQ=255)                                                 aTTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGc                                                                                              >  1:930084/1‑150 (MQ=255)                                                 aTTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGc                                                                                              >  2:177483/1‑150 (MQ=255)                                                   tCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAg                                                                                            <  1:1166738/150‑1 (MQ=255)                                                       gCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGc                                                                                       <  1:1234401/151‑1 (MQ=255)                                                       gCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGc                                                                                       <  2:1202667/151‑1 (MQ=255)                                                          ttACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGCGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCg                                                                                     >  1:752145/1‑150 (MQ=255)                                                          ttACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCg                                                                                     >  1:428745/1‑150 (MQ=255)                                                           tACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGtt                                                                                   >  2:1124288/1‑151 (MQ=255)                                                           tACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGt                                                                                    <  1:1147443/150‑1 (MQ=255)                                                            aCCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTg                                                                                  >  2:1238206/1‑151 (MQ=255)                                                            aCCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTg                                                                                  >  2:243651/1‑151 (MQ=255)                                                            aCCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTg                                                                                  >  2:1128341/1‑151 (MQ=255)                                                             ccgccgAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTgg                                                                                 >  1:404596/1‑151 (MQ=255)                                                              cgccgAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGgc                                                                                >  2:695126/1‑151 (MQ=255)                                                                ccgAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGgcgc                                                                              >  2:1171266/1‑151 (MQ=255)                                                                ccgAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGgc                                                                                <  1:602565/149‑1 (MQ=255)                                                                 cgAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCt                                                                             <  2:589576/151‑1 (MQ=255)                                                                  gAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCtt                                                                            >  1:1080825/1‑151 (MQ=255)                                                                      cgcgGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTa                                                                        <  1:736915/151‑1 (MQ=255)                                                                        cgGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTAcc                                                                      <  2:335283/151‑1 (MQ=255)                                                                            ggACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAg                                                                    >  1:335958/1‑149 (MQ=255)                                                                                   caGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACctggctg                                                           >  1:275336/1‑151 (MQ=255)                                                                                       cGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGgcgc                                                       >  2:239577/1‑151 (MQ=255)                                                                                         gACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCgg                                                     >  1:1051647/1‑151 (MQ=255)                                                                                          aCGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCgg                                                     >  1:985388/1‑150 (MQ=255)                                                                                           cGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGaa                                                   <  2:582628/151‑1 (MQ=255)                                                                                           cGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGa                                                    >  1:170789/1‑150 (MQ=255)                                                                                             ccAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACg                                                 <  1:536165/151‑1 (MQ=255)                                                                                               aGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGc                                               <  2:369825/151‑1 (MQ=255)                                                                                                  cgccTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGaa                                            <  2:94194/151‑1 (MQ=255)                                                                                                  cgccTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGaa                                            <  1:460193/151‑1 (MQ=255)                                                                                                       tCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACttt                                        <  2:1222447/150‑1 (MQ=255)                                                                                                          aTCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCtt                                    <  1:780727/151‑1 (MQ=255)                                                                                                             gTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTtat                                 <  2:755682/151‑1 (MQ=255)                                                                                                             gTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTtat                                 <  1:501252/151‑1 (MQ=255)                                                                                                                 gatgaAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAgg                              <  1:543307/150‑1 (MQ=255)                                                                                                                  atgaAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGgc                            <  2:154052/151‑1 (MQ=255)                                                                                                                  atgaAAGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGgc                            <  2:289082/151‑1 (MQ=255)                                                                                                                     aaaGACGCAAAGGATCCTTAAATGGATGAGCGCTTGCAGGCCGCGAACGAAGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGGTCGCCGGTGGCGCTTTTTACCAGACCAGGCTGACGCGGAACGGCGAACTTTTCTTTATAg                               >  1:484424/1‑145 (MQ=255)                                                                                                                     aaaGACGATACGGATCCATAATTGGCTGGGCTCTTCCCTTCCTCCTACGTCCTCCTCCTTTTTGTATGTATCCCTGACCTTCCGTTCTCCGTTTTCTCTTTTTCCCAGACCTGGCTGGCGCGGAACGGCGCACTTTTCTTTATTTGgcgag                         <  2:866674/151‑1 (MQ=255)                                                                                                                     aaaGACGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGCCCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAg                               >  1:341893/1‑145 (MQ=255)                                                                                                                          cGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAAt                    <  1:1040977/151‑1 (MQ=255)                                                                                                                          cGAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAAt                    <  1:661515/151‑1 (MQ=255)                                                                                                                           gAAAAGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAAt                    >  1:237603/1‑150 (MQ=255)                                                                                                                            aaaaGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATa                   >  1:81099/1‑150 (MQ=255)                                                                                                                               aGGATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGc               >  2:351622/1‑151 (MQ=255)                                                                                                                                ggATCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGcc              <  1:609767/151‑1 (MQ=255)                                                                                                                                   tCCATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTAt           >  2:241025/1‑151 (MQ=255)                                                                                                                                    ccATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTAtt          <  1:495608/151‑1 (MQ=255)                                                                                                                                     cATAAATGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCT‑GCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTg        <  2:587332/151‑1 (MQ=255)                                                                                                                                        aaaTGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCt      >  2:885002/1‑151 (MQ=255)                                                                                                                                        aaaTGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCt      >  2:1278669/1‑151 (MQ=255)                                                                                                                                         aaTGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTc     >  2:1261299/1‑151 (MQ=255)                                                                                                                                          aTGGCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCg    <  1:157124/151‑1 (MQ=255)                                                                                                                                            ggCTGAGCGCTTCCAGGCCGCGACCGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGCATAACGCCTATTTGCTCGa   >  1:974780/1‑150 (MQ=255)                                                                                                                                            ggCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGaa  <  1:1124248/151‑1 (MQ=255)                                                                                                                                            ggCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGa   <  2:462817/150‑1 (MQ=255)                                                                                                                                            ggCTGAGCGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGa   >  1:422727/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                 ATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAA  >  NC_000913/219290‑219579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑