Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,117,002	T→C	T42T (ACA→ACG)	wcaL ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,117,002	0	T	C	100.0%	282.1 / NA	76	T42T (ACA→ACG)	wcaL	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/43);  total (33/43)

GCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCA  >  NC_000913/2116856‑2117148                                                                                                                                                   |                                                                                                                                                   gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgtg                                                                                                                                                 >  2:914686/1‑150 (MQ=255)    tGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGc                                                                                                                                             >  2:1275106/1‑151 (MQ=255)    tGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGc                                                                                                                                             >  2:514276/1‑151 (MQ=255)       cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCtt                                                                                                                                          <  1:230217/151‑1 (MQ=255)        aGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCttt                                                                                                                                         >  2:421101/1‑151 (MQ=255)          gTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCttttt                                                                                                                                       <  1:767801/151‑1 (MQ=255)                ttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAgcg                                                                                                                                 >  1:893730/1‑151 (MQ=255)                ttACGATGAATGCCGCGCAAGGTCTGGCTGGCCCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGAGGGAGTTTTGCGTGTCGCCTTTTTGAAgcg                                                                                                                                 >  1:609079/1‑151 (MQ=255)                  aCGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGcgcg                                                                                                                               >  1:274061/1‑151 (MQ=255)                  aCGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGcgcg                                                                                                                               <  1:1246368/151‑1 (MQ=255)                           gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAAtctc                                                                                                                       <  1:1190066/150‑1 (MQ=255)                           gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATctct                                                                                                                      >  2:769410/1‑151 (MQ=255)                            ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATctct                                                                                                                      >  1:1245182/1‑150 (MQ=255)                            ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTa                                                                                                                     <  2:524189/151‑1 (MQ=255)                             cgcgCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTAc                                                                                                                    >  2:794425/1‑151 (MQ=255)                                gcAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTc                                                                                                                 >  2:521092/1‑151 (MQ=255)                                 cAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCa                                                                                                                <  1:107104/151‑1 (MQ=255)                                   aGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCa                                                                                                                <  1:935906/149‑1 (MQ=255)                                    ggTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGTAGCGCGAGAATCTCTACCTCAAAt                                                                                                             <  2:1279464/151‑1 (MQ=255)                                     gTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATc                                                                                                            <  2:381897/151‑1 (MQ=255)                                         ggctggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCat                                                                                                        <  2:191224/151‑1 (MQ=255)                                         ggctggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCat                                                                                                        <  1:558989/151‑1 (MQ=255)                                             ggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCatat                                                                                                      <  2:273986/149‑1 (MQ=255)                                             ggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCa                                                                                                    <  2:64453/151‑1 (MQ=255)                                                 cGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATaa                                                                                                <  2:993281/151‑1 (MQ=255)                                                 cGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATa                                                                                                 <  1:193035/150‑1 (MQ=255)                                                       cgcAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCgg                                                                                          >  1:1204743/1‑151 (MQ=255)                                                        gcAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCgg                                                                                          >  1:957580/1‑150 (MQ=255)                                                             ttCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAAtt                                                                                     <  2:1215039/150‑1 (MQ=255)                                                              tCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTg                                                                                   >  2:798499/1‑151 (MQ=255)                                                               cGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTgg                                                                                  <  1:39880/151‑1 (MQ=255)                                                               cGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTg                                                                                   <  2:1135720/150‑1 (MQ=255)                                                               cGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTg                                                                                   <  2:396742/150‑1 (MQ=255)                                                                  cACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGtt                                                                                >  2:753752/1‑150 (MQ=255)                                                                      ttGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAg                                                                             <  1:728284/149‑1 (MQ=255)                                                                         cccGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGa                                                                        <  1:150534/151‑1 (MQ=255)                                                                           cGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAg                                                                      <  1:654143/151‑1 (MQ=255)                                                                            gTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAgg                                                                     <  1:514248/151‑1 (MQ=255)                                                                              aGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGtt                                                                   >  2:596128/1‑151 (MQ=255)                                                                              aGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGtt                                                                   <  2:385978/151‑1 (MQ=255)                                                                               ggTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGttt                                                                  <  1:1135932/151‑1 (MQ=255)                                                                               ggTTCGGCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGttt                                                                  <  1:1261704/151‑1 (MQ=255)                                                                                   cGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTga                                                              <  1:1085323/151‑1 (MQ=255)                                                                                    gTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTgac                                                             >  2:197505/1‑151 (MQ=255)                                                                                      ccTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTgacg                                                            <  1:695152/150‑1 (MQ=255)                                                                                           aaCCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCg                                                      <  1:1069616/151‑1 (MQ=255)                                                                                           aaCCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCg                                                      <  2:869338/151‑1 (MQ=255)                                                                                             ccAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCggg                                                    <  1:433825/151‑1 (MQ=255)                                                                                                  gggTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAAttt                                               <  1:265044/151‑1 (MQ=255)                                                                                                   ggTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTc                                              >  1:796529/1‑151 (MQ=255)                                                                                                    gTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTc                                              >  2:838399/1‑150 (MQ=255)                                                                                                         ggCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGt                                           <  2:1245220/148‑1 (MQ=255)                                                                                                           cagcaAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTaaaa                                       >  1:771000/1‑150 (MQ=255)                                                                                                            agcaAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTaaaa                                       >  2:880281/1‑149 (MQ=255)                                                                                                            agcaAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAaag                                     <  1:530373/151‑1 (MQ=255)                                                                                                              caAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAaaga                                    >  1:373919/1‑150 (MQ=255)                                                                                                               aaGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAaagaa                                   <  2:1158357/150‑1 (MQ=255)                                                                                                                  gTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCg                               <  1:634405/151‑1 (MQ=255)                                                                                                                    tGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGa                              <  1:1203204/150‑1 (MQ=255)                                                                                                                     gTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGAc                             <  2:1128977/150‑1 (MQ=255)                                                                                                                       aTTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCtt                          >  2:750112/1‑151 (MQ=255)                                                                                                                         ttCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTc                         >  2:948266/1‑150 (MQ=255)                                                                                                                         ttCGTCCATGCCGCGTGAGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTc                         <  1:684296/150‑1 (MQ=255)                                                                                                                          tCGTCCATGCCGCGAGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAAGTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCtt                          <  1:477829/148‑1 (MQ=255)                                                                                                                           cGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGGTTCTGACGACAGCGGGAATTTCAGTAAAAAGAGGCCGACCTTCAtt                      >  1:20133/1‑151 (MQ=255)                                                                                                                             tCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCAttt                     >  2:276896/1‑150 (MQ=255)                                                                                                                             tCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTc                    >  2:998298/1‑151 (MQ=255)                                                                                                                              ccATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTc                    <  1:421071/150‑1 (MQ=255)                                                                                                                               cATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCAc                  >  1:888240/1‑151 (MQ=255)                                                                                                                                aTGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCAcc                 >  1:189070/1‑151 (MQ=255)                                                                                                                                      cgTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCg           <  2:578925/151‑1 (MQ=255)                                                                                                                                      cgTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCg           >  1:306895/1‑151 (MQ=255)                                                                                                                                       gTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCg           >  1:744434/1‑150 (MQ=255)                                                                                                                                         gggTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATg        >  1:399460/1‑151 (MQ=255)                                                                                                                                            tgtTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGAt         <  1:464366/147‑1 (MQ=255)                                                                                                                                              ttttGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTc   >  2:608594/1‑151 (MQ=255)                                                                                                                                                ttGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCa  >  1:894577/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCA  >  NC_000913/2116856‑2117148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑