Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,224,908	C→T	intergenic (‑38/+136)	pbpG ← / ← yohC	D‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,224,908	0	C	T	100.0%	313.1 / NA	80	intergenic (‑38/+136)	pbpG/yohC	D‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/39);  total (41/39)

CCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACG  >  NC_000913/2224762‑2225055                                                                                                                                                   |                                                                                                                                                    ccGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAc                                                                                                                                                 >  2:810414/1‑151 (MQ=255)         gAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGa                                                                                                                                          <  2:541232/150‑1 (MQ=255)           aGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGt                                                                                                                                        >  2:591229/1‑150 (MQ=255)             cGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAg                                                                                                                                     <  2:85727/151‑1 (MQ=255)             cGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAg                                                                                                                                     <  1:493991/151‑1 (MQ=255)              ggtggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAgg                                                                                                                                    <  1:85734/151‑1 (MQ=255)                    aGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTAc                                                                                                                              <  2:304563/151‑1 (MQ=255)                            ccGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTa                                                                                                                      <  2:1088790/151‑1 (MQ=255)                             catcttagCAGCGTCCTGCGGGGCAAGAGGGAAAGCCAGAATCAGGGCCAGGGTAAGTAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCt                                                                                                                       >  1:745154/8‑149 (MQ=255)                              gTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGc                                                                                                                    >  1:893274/1‑151 (MQ=255)                              gTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGc                                                                                                                    >  1:618572/1‑151 (MQ=255)                                 ttCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGa                                                                                                                  <  2:887892/150‑1 (MQ=255)                                   cGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACg                                                                                                                <  2:544930/150‑1 (MQ=255)                                   cGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGc                                                                                                               >  2:636831/1‑151 (MQ=255)                                   cGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGc                                                                                                               >  1:242482/1‑151 (MQ=255)                                     cAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCca                                                                                                             <  1:431889/151‑1 (MQ=255)                                      aaCGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcat                                                                                                            >  1:1027095/1‑151 (MQ=255)                                        cGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatca                                                                                                          <  1:15779/151‑1 (MQ=255)                                           ccTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATaa                                                                                                       <  2:45753/151‑1 (MQ=255)                                                  tGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCa                                                                                                 >  2:979200/1‑150 (MQ=255)                                                   gCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaa                                                                                                <  1:620137/150‑1 (MQ=255)                                                     aaaaGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaaaa                                                                                             <  2:1265052/151‑1 (MQ=255)                                                     aaaaGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaaaa                                                                                             <  2:1230294/151‑1 (MQ=255)                                                     aaaaGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCAGAGTAAGGATTTACTGAGGCTAGCGACGGCATCATAACGAGCaaa                                                                                               >  2:240853/1‑149 (MQ=255)                                                          gCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGTCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCg                                                                                        <  2:705626/151‑1 (MQ=255)                                                           cacaGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGa                                                                                       <  2:706127/151‑1 (MQ=255)                                                                ccagcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTc                                                                                  >  2:597482/1‑151 (MQ=255)                                                                ccagcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTc                                                                                  <  2:1171781/151‑1 (MQ=255)                                                                 cagcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCa                                                                                 <  2:956297/151‑1 (MQ=255)                                                                    cATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAg                                                                              <  1:815187/151‑1 (MQ=255)                                                                     aTCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAgg                                                                             >  2:393795/1‑151 (MQ=255)                                                                       cAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGgag                                                                           <  1:636796/151‑1 (MQ=255)                                                                        aGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGAGCGATCGTCAAAgg                                                                             >  1:727974/1‑148 (MQ=255)                                                                         gggCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGgaga                                                                          >  1:677017/1‑150 (MQ=255)                                                                             cAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCg                                                                      >  2:970990/1‑150 (MQ=255)                                                                             cAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGt                                                                     <  1:596902/151‑1 (MQ=255)                                                                              aGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTg                                                                    >  1:574702/1‑151 (MQ=255)                                                                               ggCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGa                                                                   <  1:1264587/151‑1 (MQ=255)                                                                                   aaataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGaa                                                               <  1:290565/151‑1 (MQ=255)                                                                                       aaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgc                                                           <  1:882577/151‑1 (MQ=255)                                                                                       aaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgc                                                           >  2:675599/1‑151 (MQ=255)                                                                                        aaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgct                                                          >  1:70308/1‑151 (MQ=255)                                                                                        aaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgct                                                          >  2:7799/1‑151 (MQ=255)                                                                                           aaaCTCGAAATTTCGGCATGATGGGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGACTCGTGAGGATAtgctgc                                                        >  1:763259/1‑150 (MQ=255)                                                                                           aaaCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgctgc                                                        >  2:1164406/1‑150 (MQ=255)                                                                                            aaCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAt                                                      >  2:950456/1‑151 (MQ=255)                                                                                            aaCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAt                                                      <  2:736805/151‑1 (MQ=255)                                                                                             aCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAtt                                                     >  2:367551/1‑151 (MQ=255)                                                                                                 gAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTg                                                 >  1:356568/1‑151 (MQ=255)                                                                                                 gAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTg                                                 <  1:1255645/151‑1 (MQ=255)                                                                                                  aaaTTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGAGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGAACGTCAAAAGAGAATCGTGAGGAAATGCTGCATTGCtta                                                <  1:277299/151‑3 (MQ=255)                                                                                                   aaTTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGAc                                               >  1:1210007/1‑151 (MQ=255)                                                                                                   aaTTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGAc                                               >  1:271482/1‑151 (MQ=255)                                                                                                        cGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTa                                          <  2:1117467/151‑1 (MQ=255)                                                                                                         ggCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTAc                                         <  2:719345/151‑1 (MQ=255)                                                                                                          gCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACg                                        >  2:536964/1‑151 (MQ=255)                                                                                                          acATGATGAGCATACAGATAGTGTTTCCCGCGCTCGGGCTGTGCACCGCCGGGGTAAGGATTTACTGCGCCTAGCGACGCCATCATAACGAGCAAACCGTGCGCCCGTCATAGGTGATTCGTGAGAGAATGCTGCATTGATGACATTTACg                                        >  1:1278617/2‑151 (MQ=255)                                                                                                            atgatgAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTACACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGcc                                      <  2:543950/151‑1 (MQ=255)                                                                                                              gatgaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGcc                                      >  1:451764/1‑149 (MQ=255)                                                                                                                tgaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGc                                   >  2:242193/1‑150 (MQ=255)                                                                                                                tgaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGc                                   >  1:374810/1‑150 (MQ=255)                                                                                                                 gaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCa                                  >  2:1282961/1‑150 (MQ=255)                                                                                                                 gaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCa                                  >  1:938048/1‑150 (MQ=255)                                                                                                                   gCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATg                               >  1:571472/1‑151 (MQ=255)                                                                                                                       tCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCaa                            <  2:1179571/150‑1 (MQ=255)                                                                                                                        cAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACg                          <  1:954376/151‑1 (MQ=255)                                                                                                                         aGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGt                         >  2:962597/1‑151 (MQ=255)                                                                                                                         aGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGt                         >  1:140833/1‑151 (MQ=255)                                                                                                                         aGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGt                         <  1:1243214/151‑1 (MQ=255)                                                                                                                         aGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGt                         >  1:781341/1‑151 (MQ=255)                                                                                                                             aGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCaa                      >  1:910739/1‑150 (MQ=255)                                                                                                                                 gTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAAc                 <  1:393766/151‑1 (MQ=255)                                                                                                                                 gTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAAc                 <  2:457851/151‑1 (MQ=255)                                                                                                                                       gcgcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTa           <  1:591199/151‑1 (MQ=255)                                                                                                                                         gcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt            >  1:141510/1‑148 (MQ=255)                                                                                                                                         gcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGa         <  2:1144482/151‑1 (MQ=255)                                                                                                                                           aCGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAAc       >  1:725004/1‑151 (MQ=255)                                                                                                                                           aCGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAAc       <  2:891696/151‑1 (MQ=255)                                                                                                                                              ggTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACaaa    <  1:754152/151‑1 (MQ=255)                                                                                                                                                ttGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACg  >  2:677625/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACG  >  NC_000913/2224762‑2225055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑