Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,230,378	A→G	V3A (GTG→GCG)	dusC ←	tRNA‑dihydrouridine synthase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,230,378	0	A	G	100.0%	323.8 / NA	91	V3A (GTG→GCG)	dusC	tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/42);  total (49/42)

CAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATG  >  NC_000913/2230232‑2230524                                                                                                                                                   |                                                                                                                                                   cAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCAGCGGTGCCAGTAacgcac                                                                                                                                                 <  1:259245/150‑1 (MQ=255)  aGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAacgcacgc                                                                                                                                               >  2:690168/1‑151 (MQ=255)     gCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCata                                                                                                                                            <  1:342913/151‑1 (MQ=255)     gCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCat                                                                                                                                             >  1:1055954/1‑150 (MQ=255)       aaaTGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATatc                                                                                                                                          >  2:1048505/1‑151 (MQ=255)        aaTGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATatca                                                                                                                                         <  2:936176/151‑1 (MQ=255)        aaTGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATatca                                                                                                                                         <  2:1204304/151‑1 (MQ=255)        aaTGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGACAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATatc                                                                                                                                          >  1:908213/1‑150 (MQ=255)         aTGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATatcat                                                                                                                                        <  1:1039043/151‑1 (MQ=255)            cGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGGGATGCACAGATCGTAGTCGTTAATTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGTCAGTAACGCACGCATAtcatca                                                                                                                                      >  1:597519/1‑150 (MQ=255)             gATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCAcc                                                                                                                                    >  2:1080505/1‑151 (MQ=255)                 aaaGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCa                                                                                                                                <  2:791842/151‑1 (MQ=255)                 aaaGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTAGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCAcc                                                                                                                                    >  1:383597/1‑147 (MQ=255)                   aGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCaaa                                                                                                                              <  1:581890/151‑1 (MQ=255)                     aCTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCaaaa                                                                                                                             >  2:282760/1‑150 (MQ=255)                          tACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATg                                                                                                                        >  2:1095519/1‑150 (MQ=255)                           aCCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATg                                                                                                                        >  2:1202915/1‑149 (MQ=255)                             cGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGgc                                                                                                                    <  2:785286/151‑1 (MQ=255)                             cGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGgc                                                                                                                    >  2:1138462/1‑151 (MQ=255)                                cagcagTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCt                                                                                                                 >  1:982647/1‑151 (MQ=255)                                    agTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGt                                                                                                             >  1:899708/1‑151 (MQ=255)                                     gTTGATCCGCCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAGTTCACACACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCCATGt                                                                                                             <  1:675693/150‑1 (MQ=255)                                     gTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGtt                                                                                                            >  2:132470/1‑151 (MQ=255)                                        gATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCGCCCGCAAAAAAATGAGGCGCTATGTTAg                                                                                                          <  1:724717/150‑1 (MQ=255)                                          tCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAgcgc                                                                                                       >  2:835398/1‑151 (MQ=255)                                           ccaccaCGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGcc                                                                                                      >  1:828483/1‑151 (MQ=255)                                            caccacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCt                                                                                                     <  2:181931/151‑1 (MQ=255)                                             accacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCt                                                                                                     >  2:709031/1‑150 (MQ=255)                                               cacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                  >  2:255419/1‑151 (MQ=255)                                               cacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                  >  2:374413/1‑151 (MQ=255)                                               cacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                  <  2:540337/151‑1 (MQ=255)                                               cacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGACAGCAATTCACGCACCAGAGAGCCAAGCACTCCCTCCATCGGTGCCAGTAACGCCCGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                  >  1:615218/1‑151 (MQ=255)                                                  gcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTc                                                                                               >  1:602201/1‑151 (MQ=255)                                                  gcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGGCAGCAACTCACGCTCCAGCGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACCCCTATCATCGCCCCCAAACAAATGAGGCGCTATGTTAGCGATTATGGTc                                                                                               <  1:1278801/151‑1 (MQ=255)                                                   cgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTc                                                                                               >  2:744290/1‑150 (MQ=255)                                                   cgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCa                                                                                              <  1:472901/151‑1 (MQ=255)                                                      aCAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCg                                                                                           >  1:284794/1‑151 (MQ=255)                                                       cAAACTCGGTGATGCACAGATCATAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCgg                                                                                          <  1:1088942/151‑1 (MQ=255)                                                             cGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTa                                                                                      <  2:1270358/149‑1 (MQ=255)                                                               gTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAgg                                                                                  <  2:982686/151‑1 (MQ=255)                                                                tGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGt                                                                                 >  2:943574/1‑151 (MQ=255)                                                                     cacaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGc                                                                             >  2:631444/1‑150 (MQ=255)                                                                     cacaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCa                                                                            <  2:777725/151‑1 (MQ=255)                                                                       caGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaaa                                                                          <  2:1159728/151‑1 (MQ=255)                                                                        aGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaa                                                                           >  2:32739/1‑149 (MQ=255)                                                                        aGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaa                                                                           >  2:366377/1‑149 (MQ=255)                                                                        aGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaa                                                                           >  1:1122209/1‑149 (MQ=255)                                                                         gATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAaga                                                                        <  1:696010/151‑1 (MQ=255)                                                                             gTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                     >  1:719825/1‑150 (MQ=255)                                                                             gTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                     >  1:1035271/1‑150 (MQ=255)                                                                             gTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGa                                                                    <  1:297542/151‑1 (MQ=255)                                                                             gTAGTCGTAAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCAATCGGTGCCAGGAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCATATGGTCAGCGGATTAAAGGCCTGCAAAGAggg                                                                     >  1:690647/1‑150 (MQ=255)                                                                                gTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtg                                                                 <  2:917439/151‑1 (MQ=255)                                                                                  cGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                               <  2:848194/151‑1 (MQ=255)                                                                                  cGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                               <  1:1080396/151‑1 (MQ=255)                                                                                   gTTAACTTCGGTCAGCAATTAACGCACCAGTGAGTCAAGCACTCCCTCCATCGGCGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTGTGCAAAGAGGGATtgctg                                                              >  1:226160/1‑151 (MQ=255)                                                                                     tAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgctgc                                                             >  2:287832/1‑150 (MQ=255)                                                                                     tAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgctg                                                              <  1:1091429/149‑1 (MQ=255)                                                                                     tAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgctg                                                              >  2:1091470/1‑149 (MQ=255)                                                                                       aCTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                          <  2:780110/151‑1 (MQ=255)                                                                                       aCTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaa                                                           >  2:763147/1‑150 (MQ=255)                                                                                          tCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACt                                                        <  2:454245/150‑1 (MQ=255)                                                                                            ggTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCa                                                     <  2:575448/151‑1 (MQ=255)                                                                                             gTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCTCGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCaa                                                    <  1:1252820/151‑1 (MQ=255)                                                                                              tCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCaa                                                    >  2:196301/1‑150 (MQ=255)                                                                                               cAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAt                                                   >  1:867265/1‑150 (MQ=255)                                                                                               cAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATc                                                  >  1:540726/1‑151 (MQ=255)                                                                                                  cAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCct                                               >  2:631819/1‑151 (MQ=255)                                                                                                    aTTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctc                                              >  1:781719/1‑150 (MQ=255)                                                                                                      tCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctct                                           >  2:76379/1‑151 (MQ=255)                                                                                                        aCGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTAt                                         <  2:1323/151‑1 (MQ=255)                                                                                                         cGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATg                                        <  2:570118/151‑1 (MQ=255)                                                                                                            aCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCtt                                     <  1:1032754/151‑1 (MQ=255)                                                                                                            aCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCtt                                     >  2:1055104/1‑151 (MQ=255)                                                                                                            aCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCtt                                     >  2:1224750/1‑151 (MQ=255)                                                                                                              cAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGa                                   <  2:1144165/151‑1 (MQ=255)                                                                                                               agagagTCAAGCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGAt                                  >  2:286805/1‑151 (MQ=255)                                                                                                                    gTCAAGCACTCCCTCCATCTGTGACAGTAACGCTCGGATATCATCACCCGCAAAAAAATGAGGCGCTATGTTATCTCCTATGGTCAGCAGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAGCCCTCTCTATGCTTGATGTAt                              <  1:96110/150‑1 (MQ=255)                                                                                                                         gCACTCCCTCCATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTg                         >  1:352466/1‑150 (MQ=255)                                                                                                                              ccctccATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTc                    <  1:375662/150‑1 (MQ=255)                                                                                                                              ccctccATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCg                   >  1:1214310/1‑151 (MQ=255)                                                                                                                                  ccATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgc               <  1:557665/151‑1 (MQ=255)                                                                                                                                  ccATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                >  2:262895/1‑150 (MQ=255)                                                                                                                                  ccATCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                >  1:736801/1‑150 (MQ=255)                                                                                                                                    aTCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcgc             <  1:434281/151‑1 (MQ=255)                                                                                                                                    aTCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcgc             <  2:600598/151‑1 (MQ=255)                                                                                                                                    aTCGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcgc             <  2:225646/151‑1 (MQ=255)                                                                                                                                      cGGTGCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCAt           >  2:288192/1‑151 (MQ=255)                                                                                                                                          gCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCg       <  2:161596/151‑1 (MQ=255)                                                                                                                                          gCCAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCg       >  1:1150299/1‑151 (MQ=255)                                                                                                                                           ccAGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGt      <  2:909115/151‑1 (MQ=255)                                                                                                                                             aGTAACGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTaa    <  2:1211041/151‑1 (MQ=255)                                                                                                                                                aaCGCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATg  <  2:844254/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATG  >  NC_000913/2230232‑2230524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑