| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,246,756 | T→C | N5S (AAC→AGC) | cirA ← | colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,246,756 | 0 | T | C | 98.3% | 211.9 / NA | 60 | N5S (AAC→AGC) | cirA | colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (35/24); minor base G (1/0); total (36/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAAC > NC_000913/2246608‑2246900 | aaTGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACCAACACAGCCCGACCCGTACGAAAGGGCTc > 2:564654/1‑151 (MQ=255) aTGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCa < 1:637170/151‑1 (MQ=255) aTGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCa < 2:405097/151‑1 (MQ=255) tGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCa > 1:619813/1‑150 (MQ=255) gACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCaa < 1:657708/150‑1 (MQ=255) tGATACTGGCAGGTGCATCTTTAAGGTTTTGTTCCACGGAAAATTAAGTGACAACCATCGTTTCGCCATCATCATTGTCCGCTAACACAAGGCATGCACAAGAAATAGCGGACAAACACAGCCCCACCCGCACGAAAAGGGTCaaac > 1:500938/1‑145 (MQ=255) tGATACTGGCAGGTGCATCTTTAAGAATTTTGTCCACTGAAGATGCCAGGCCAACCAACCTTTCGCCATCATCATCGACCCCGAACACAAGCCATGCACAAGAAATAACGCAGAAACACAGCCCGATCCGTCCGCAACGGCTCGACCtca > 1:26352/1‑148 (MQ=255) gATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTaa < 1:1164681/149‑1 (MQ=255) aCTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACAtt < 2:118877/151‑1 (MQ=255) aCTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACAt < 1:715005/150‑1 (MQ=255) aCTGGCAGGTGCAACTTTAAACTTTTGTTTCACGGGAGATGCAGTGACAACCATCGTTTTGCCAACATCATCGACCGCTAACACAGGCCATTCACAAGAAAAAGCGGACAAACACAACCCGACCCCTACGAAAAGGCCCAACCTAAACAtt > 1:824639/1‑151 (MQ=255) ggCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTc < 2:802300/149‑1 (MQ=255) cAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCat > 2:310838/1‑150 (MQ=255) cAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCat > 1:1254299/1‑150 (MQ=255) aGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCatat < 1:262201/151‑1 (MQ=255) ggTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATAtc < 2:876247/151‑1 (MQ=255) gCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATAtctc < 2:595408/150‑1 (MQ=255) cTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCAGCACGAAAGGGCTCAACCTAAACATTCCATATCTCAATg > 1:564899/1‑150 (MQ=255) ttGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACg > 2:1208314/1‑151 (MQ=255) gTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGa > 1:1110959/1‑150 (MQ=255) ttCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaa > 1:267220/1‑150 (MQ=255) tCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaa < 2:1228163/150‑1 (MQ=255) aCGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGGCCGCTAACACAGGCCATGCACAAGCAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACAGTCCATATCTCCATGAGGTAACTACg < 2:564929/144‑1 (MQ=255) aCGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaataa < 2:330016/151‑1 (MQ=255) cGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaataa > 1:1060544/1‑150 (MQ=255) ggAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaataaa > 1:503301/1‑150 (MQ=255) gAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaataaaat > 1:297087/1‑151 (MQ=255) gATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATggg < 1:1259459/151‑1 (MQ=255) aTGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGt > 2:25236/1‑151 (MQ=255) aGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATc < 1:25241/151‑1 (MQ=255) tGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGc > 2:639490/1‑151 (MQ=255) gACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCt > 2:635888/1‑151 (MQ=255) aCCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTcaca < 1:919859/151‑1 (MQ=255) tCGTTTCGTCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACAtctt > 2:332707/1‑151 (MQ=255) acGTTTAGCCATCATCATCGACCGCTAACACAAGTCATGCACAAGAAATAGCGGACAAACCCAGCCCGAGCCGTAAGAAAGAGCACAACCTAAGCATTCAATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACAtc > 1:245225/2‑149 (MQ=255) atcatcatcGACCGCTAACACAGGCCATGCACAAGAACTAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTcc > 1:636093/1‑151 (MQ=255) catcatcGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTcccc > 1:78211/1‑151 (MQ=255) catcGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCtt < 1:287253/150‑1 (MQ=255) cGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCg < 2:888922/150‑1 (MQ=255) ccGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACg > 2:194133/1‑151 (MQ=255) cGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACgg > 1:458693/1‑151 (MQ=255) cacaGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCAt > 1:333126/1‑151 (MQ=255) acaGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATc > 1:1022594/1‑151 (MQ=255) aGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATcgc < 2:722167/151‑1 (MQ=255) aGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATcgc < 2:1242887/151‑1 (MQ=255) ggCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATcgcg > 1:406595/1‑151 (MQ=255) tGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg > 2:1240543/1‑150 (MQ=255) gCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGa < 1:882549/150‑1 (MQ=255) aaTAGCGGACAAACACAGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTAttttt > 1:1265457/1‑151 (MQ=255) aaTAGCGCACAAACACAGCCCGACCCGTACGAAAGGGCCCCACCTAAACATTCCATATATCCATGAGGTAACTACGACACTAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCACCATCGCGGTGGACTTAttttt > 1:1173914/1‑151 (MQ=255) acaGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGatc > 2:1273052/1‑150 (MQ=255) aGCCCGACCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGatcatc > 1:94915/1‑151 (MQ=255) aCCCGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGa > 1:553744/1‑150 (MQ=255) cccGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGaaa < 2:298686/151‑1 (MQ=255) cccGTACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGaa > 2:326130/1‑150 (MQ=255) tACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCa < 2:220540/130‑1 (MQ=255) tACGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCa > 1:220536/1‑130 (MQ=255) aCGAAAGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCgg < 2:739053/151‑1 (MQ=255) aaaGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAAc > 2:563247/1‑151 (MQ=255) aGGGCTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAAc > 2:1125268/1‑149 (MQ=255) | AATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAAC > NC_000913/2246608‑2246900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |