Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,603,573	C→T	P586S (CCG→TCG)	hyfB →	hydrogenase 4, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,603,573	0	C	T	100.0%	306.7 / NA	80	P586S (CCG→TCG)	hyfB	hydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/36);  total (44/36)

ACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTG  >  NC_000913/2603428‑2603719                                                                                                                                                  |                                                                                                                                                   actactGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCt                                                                                                                                                <  1:186365/150‑1 (MQ=255)  ctactGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCt                                                                                                                                                <  1:763314/149‑1 (MQ=255)    actGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCgt                                                                                                                                            <  2:499749/151‑1 (MQ=255)    actGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCg                                                                                                                                             >  1:328432/1‑150 (MQ=255)      tGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCgtgt                                                                                                                                          >  2:1256916/1‑151 (MQ=255)       ggCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCgtgt                                                                                                                                          <  2:390775/150‑1 (MQ=255)       ggCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGtgtg                                                                                                                                         <  2:944446/151‑1 (MQ=255)                    ctgcCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTc                                                                                                                                 >  2:1169158/1‑146 (MQ=255)                    ctgcCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTgcgc                                                                                                                            >  2:891985/1‑151 (MQ=255)                     tgcCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCt                                                                                                                           >  2:1194760/1‑151 (MQ=255)                     tgcCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCt                                                                                                                           >  2:737074/1‑151 (MQ=255)                      gcCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTa                                                                                                                          <  2:710477/151‑1 (MQ=255)                       ccTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTAt                                                                                                                         <  2:247644/151‑1 (MQ=255)                          ggCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCGCAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTAtt                                                                                                                        <  2:423905/149‑1 (MQ=255)                          ggCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTAttt                                                                                                                       <  1:418948/150‑1 (MQ=255)                             cTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGGGGGGTCTTTTCTGCGCTATTTCg                                                                                                                     >  1:70771/1‑149 (MQ=255)                               ttACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTc                                                                                                                   <  2:923589/149‑1 (MQ=255)                               ttACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCt                                                                                                                  >  2:598797/1‑150 (MQ=255)                                    ggCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGaca                                                                                                            <  2:684129/151‑1 (MQ=255)                                    ggCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGa                                                                                                              <  1:1070030/149‑1 (MQ=255)                                        gtggtgTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGacaac                                                                                                          <  2:1244507/149‑1 (MQ=255)                                           gtgtCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTc                                                                                                     <  1:725200/151‑1 (MQ=255)                                             gtCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCg                                                                                                    >  2:87155/1‑150 (MQ=255)                                               cgatCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGAcc                                                                                                 <  2:691081/148‑1 (MQ=255)                                                     cgccgcgccgCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGAGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGc                                                                                           >  1:322845/1‑151 (MQ=255)                                                     cgccgcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGc                                                                                           >  2:118727/1‑151 (MQ=255)                                                        cgcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGa                                                                                        <  2:265282/151‑1 (MQ=255)                                                         gcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTg                                                                                         >  2:305715/1‑149 (MQ=255)                                                            cAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCt                                                                                    <  2:1035707/151‑1 (MQ=255)                                                             cgCGTTTCGTCGCAAAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATCGCCCCGTCAGGCAATGGCGCGATGCAGTCGCCGAGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACCGCTCGACCCTACGCTGAg                                                                                       >  1:331247/2‑147 (MQ=255)                                                              gCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCtaa                                                                                  <  1:50203/151‑1 (MQ=255)                                                               cGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCtaaa                                                                                 <  1:1049978/151‑1 (MQ=255)                                                                gTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCtaaat                                                                                >  1:1283515/1‑151 (MQ=255)                                                                  ttCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCtaaata                                                                               <  2:1153537/150‑1 (MQ=255)                                                                   tcgtcgCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCtaaataa                                                                              <  1:1006151/150‑1 (MQ=255)                                                                    cgtcgCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAg                                                                            <  2:1046241/151‑1 (MQ=255)                                                                     gtcgCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAgg                                                                           <  2:636714/151‑1 (MQ=255)                                                                     gtcgCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAgg                                                                           >  2:17107/1‑151 (MQ=255)                                                                      tcgCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGt                                                                          >  2:432530/1‑151 (MQ=255)                                                                       cgCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTc                                                                         >  2:228018/1‑151 (MQ=255)                                                                            aGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTgcg                                                                    >  1:928302/1‑151 (MQ=255)                                                                            aGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTgcg                                                                    <  1:73355/151‑1 (MQ=255)                                                                             ggAGATGCCTGGGCATGCGGCTTCGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTgcgc                                                                   >  1:246379/1‑151 (MQ=255)                                                                              gagaTGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCa                                                                  <  2:743421/151‑1 (MQ=255)                                                                               agaTGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCAc                                                                 >  1:1078868/1‑151 (MQ=255)                                                                                  tGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTc                                                              >  1:1083696/1‑151 (MQ=255)                                                                                     cTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCAcc                                                           >  1:399867/1‑151 (MQ=255)                                                                                      tGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCg                                                          >  2:1235529/1‑151 (MQ=255)                                                                                      tGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGCCTTTTCTGCCCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCg                                                          >  1:96263/1‑151 (MQ=255)                                                                                         gCATGCGGCTGCGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCa                                                       <  1:34043/151‑1 (MQ=255)                                                                                         gCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCa                                                       <  2:66914/151‑1 (MQ=255)                                                                                          cATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGcc                                                        >  1:1016832/1‑149 (MQ=255)                                                                                            tGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAg                                                      <  2:331273/149‑1 (MQ=255)                                                                                              cGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAggg                                                    >  1:333778/1‑149 (MQ=255)                                                                                              cGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCt                                                  >  2:251130/1‑151 (MQ=255)                                                                                                 cTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTc                                                 >  2:690236/1‑149 (MQ=255)                                                                                                  tACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTc                                                 >  1:404108/1‑148 (MQ=255)                                                                                                   aCGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCag                                               <  2:349289/149‑1 (MQ=255)                                                                                                       cTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGcac                                          >  2:1200224/1‑150 (MQ=255)                                                                                                        tGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGcac                                          >  1:44928/1‑149 (MQ=255)                                                                                                         gggAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAg                                        <  2:821298/150‑1 (MQ=255)                                                                                                          ggAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGa                                       >  1:479519/1‑150 (MQ=255)                                                                                                           gAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAAc                                     >  2:413467/1‑151 (MQ=255)                                                                                                           gAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAAc                                     <  1:6883/151‑1 (MQ=255)                                                                                                             aaaTGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAAccc                                   >  1:217906/1‑151 (MQ=255)                                                                                                                    aTGGCCCCGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTggg                            >  2:744277/1‑151 (MQ=255)                                                                                                                        ccccGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGa                        <  2:893198/151‑1 (MQ=255)                                                                                                                         cccGTCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAg                       <  1:525959/151‑1 (MQ=255)                                                                                                                             tCAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCggg                   >  2:464666/1‑151 (MQ=255)                                                                                                                              cAGGCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACTACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGATCGGGt                  >  1:1248575/1‑151 (MQ=255)                                                                                                                                ggCAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGa                <  2:719330/151‑1 (MQ=255)                                                                                                                                  cAATGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATc              >  1:905336/1‑151 (MQ=255)                                                                                                                                   aaTGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATcc             >  1:523608/1‑151 (MQ=255)                                                                                                                                   aaTGGCGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATcc             >  1:212133/1‑151 (MQ=255)                                                                                                                                        cGTGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGcccc        >  2:256780/1‑151 (MQ=255)                                                                                                                                          tGATGCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCAt      >  2:734943/1‑151 (MQ=255)                                                                                                                                              gCAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTg  <  2:351888/151‑1 (MQ=255)                                                                                                                                               cAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGTACGTCACCGCCAGGGCTCAGAGCACAGAGCCCTTCTGGGATCCGCGGGTGATCCCCACCATCGt   <  1:82516/149‑1 (MQ=255)                                                                                                                                               cAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGt   >  1:640468/1‑149 (MQ=255)                                                                                                                                               cAGTCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTg  >  2:336346/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                   ACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTG  >  NC_000913/2603428‑2603719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑