Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	348,596	C→A	intergenic (‑153/‑86)	prpR ← / → prpB	propionate catabolism operon regulatory protein/2‑methylisocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	348,596	0	C	A	100.0%	307.4 / NA	78	intergenic (‑153/‑86)	prpR/prpB	propionate catabolism operon regulatory protein/2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/37);  total (41/37)

CACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGAAAATCCATTGCA  >  NC_000913/348448‑348743                                                                                                                                                     |                                                                                                                                                    cACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAAt                                                                                                                                                   <  1:1024219/151‑1 (MQ=255) cACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAAt                                                                                                                                                   <  2:103670/151‑1 (MQ=255)  aCCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAAtt                                                                                                                                                  <  2:801332/151‑1 (MQ=255)    cGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAAttt                                                                                                                                                 >  2:316082/1‑150 (MQ=255)     gCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTaa                                                                                                                                               <  2:380393/151‑1 (MQ=255)      cAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAAt                                                                                                                                              >  1:97372/1‑151 (MQ=255)      cAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAAt                                                                                                                                              <  2:623514/151‑1 (MQ=255)       aaaGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAAtt                                                                                                                                             >  1:1019644/1‑151 (MQ=255)         aGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAAtttt                                                                                                                                           >  1:12636/1‑151 (MQ=255)          gTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAAtttt                                                                                                                                           >  2:1283443/1‑150 (MQ=255)             aaGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTttatta                                                                                                                                       <  1:166458/151‑1 (MQ=255)             aaGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTttatta                                                                                                                                       <  1:167764/151‑1 (MQ=255)             aaGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTttatt                                                                                                                                        <  1:186736/150‑1 (MQ=255)               gAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAg                                                                                                                                     <  2:315092/151‑1 (MQ=255)                 aaCCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGc                                                                                                                                   <  2:442995/151‑1 (MQ=255)                  aCCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCa                                                                                                                                  <  2:523974/151‑1 (MQ=255)                   ccGAATATTGGGTTTGGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCaa                                                                                                                                 <  2:716797/151‑1 (MQ=255)                   ccGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCaa                                                                                                                                 <  2:540403/151‑1 (MQ=255)                    cgcatatTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAAt                                                                                                                                >  1:1032248/4‑151 (MQ=255)                    cGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAAt                                                                                                                                <  2:484517/151‑1 (MQ=255)                      aaTATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATtg                                                                                                                              <  1:1186817/151‑1 (MQ=255)                          ttGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGc                                                                                                                          >  2:540841/1‑151 (MQ=255)                           tGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGc                                                                                                                          >  2:968036/1‑150 (MQ=255)                            gggTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGca                                                                                                                         <  1:409913/150‑1 (MQ=255)                              gTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCacac                                                                                                                      >  2:6344/1‑151 (MQ=255)                                ttAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACAccc                                                                                                                    >  2:34516/1‑151 (MQ=255)                                          ttCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTg                                                                                                           <  2:536218/150‑1 (MQ=255)                                          ttCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGt                                                                                                          >  2:739279/1‑151 (MQ=255)                                          ttCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGt                                                                                                          >  1:422633/1‑151 (MQ=255)                                             aTAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCtt                                                                                                       >  1:1271629/1‑151 (MQ=255)                                             aTAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCtt                                                                                                       >  2:609718/1‑151 (MQ=255)                                             aTAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCtt                                                                                                       >  1:440986/1‑151 (MQ=255)                                               aaTTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCttt                                                                                                      >  1:14337/1‑150 (MQ=255)                                               aaTTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTa                                                                                                     <  1:516263/151‑1 (MQ=255)                                                aTTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTAt                                                                                                    <  1:119223/151‑1 (MQ=255)                                                     tgCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAAc                                                                                                <  2:631534/150‑1 (MQ=255)                                                      gCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACg                                                                                               <  2:1282289/150‑1 (MQ=255)                                                      gCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGc                                                                                              >  1:1010957/1‑151 (MQ=255)                                                          tGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAAt                                                                                          >  1:104811/1‑151 (MQ=255)                                                            aaaCGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATaa                                                                                        <  2:1035321/151‑1 (MQ=255)                                                             aaCGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAAc                                                                                       >  2:725636/1‑151 (MQ=255)                                                              aCGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAAc                                                                                       >  2:286655/1‑150 (MQ=255)                                                                   gTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTg                                                                                 >  2:62201/1‑151 (MQ=255)                                                                     gAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGa                                                                                <  2:3135/150‑1 (MQ=255)                                                                     gAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGa                                                                                <  1:21899/150‑1 (MQ=255)                                                                     gAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGAt                                                                               >  1:1055668/1‑151 (MQ=255)                                                                       aaCATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATGTTATTAAGGCAATTGTGGCACACCGCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATaa                                                                             <  2:1178705/151‑1 (MQ=255)                                                                         cATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAAc                                                                            <  2:3627/150‑1 (MQ=255)                                                                          aTTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTTTCTTTATCAACCTAAATAACAATTTGATGACa                                                                           <  1:778384/150‑1 (MQ=255)                                                                          aTTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACaa                                                                          <  2:348662/151‑1 (MQ=255)                                                                             gCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAgg                                                                       <  1:710706/151‑1 (MQ=255)                                                                             gCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAgg                                                                       <  2:610945/151‑1 (MQ=255)                                                                                         aaCTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGtc                                                           >  2:382856/1‑151 (MQ=255)                                                                                         aaCTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGt                                                            <  1:540813/150‑1 (MQ=255)                                                                                            tGACACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGtct                                                          >  1:449750/1‑149 (MQ=255)                                                                                             gAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGtctc                                                         <  2:1042099/149‑1 (MQ=255)                                                                                                aCGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTacac                                                    >  1:1173133/1‑151 (MQ=255)                                                                                                      atTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTcc                                               <  2:471130/150‑1 (MQ=255)                                                                                                       tttGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTcc                                               >  1:954038/1‑149 (MQ=255)                                                                                                           cGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTaa                                         <  1:141175/151‑1 (MQ=255)                                                                                                            ggATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTaa                                         >  1:367868/1‑150 (MQ=255)                                                                                                                 aGTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGtt                                   >  2:1015726/1‑151 (MQ=255)                                                                                                                  gTTCATGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGttt                                  >  1:986020/1‑151 (MQ=255)                                                                                                                      aTGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTcgcg                              <  1:972595/151‑1 (MQ=255)                                                                                                                       tGACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCgcgc                             >  2:958852/1‑151 (MQ=255)                                                                                                                        gACTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCt                            >  2:43705/1‑151 (MQ=255)                                                                                                                          cTTTATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGc                          >  1:357427/1‑151 (MQ=255)                                                                                                                            ttATCTCTAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCa                         <  2:903951/150‑1 (MQ=255)                                                                                                                                ctctAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGAc                    >  2:897011/1‑151 (MQ=255)                                                                                                                                 tctAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGAc                    >  2:470451/1‑150 (MQ=255)                                                                                                                                 tctAACAAATTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGAc                    >  2:1000684/1‑150 (MQ=255)                                                                                                                                       aaaTTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGaa             <  2:530550/151‑1 (MQ=255)                                                                                                                                       aaaTTGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGa              >  1:948668/1‑150 (MQ=255)                                                                                                                                           tGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGAAAATc         >  1:73761/1‑151 (MQ=255)                                                                                                                                           tGAAATTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGAAAATc         >  1:730975/1‑151 (MQ=255)                                                                                                                                             aaaTTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGAAAATc         >  2:597358/1‑149 (MQ=255)                                                                                                                                              aaTTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGAAAATCCAt      >  2:299267/1‑151 (MQ=255)                                                                                                                                               aTTAAAAATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGAAAATCCAtt     >  2:37701/1‑151 (MQ=255)                                                                                                                                                  aaaaATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGAAAATCCATTGCa  <  2:812463/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCGCGCTGCACTGACTAAAGAAAATCCATTGCA  >  NC_000913/348448‑348743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑