Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,903,235	C→T	A447V (GCC→GTC)	ygcE →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,903,235	0	C	T	98.7%	298.4 / NA	77	A447V (GCC→GTC)	ygcE	putative kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (38/38);  minor base G (0/1);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCACGACGTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAA  >  NC_000913/2903088‑2903382                                                                                                                                                    |                                                                                                                                                    cGGCACGACGTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCaa                                                                                                                                                  <  1:467125/151‑1 (MQ=255)  ggCACGACGTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCaaa                                                                                                                                                 <  2:537500/151‑1 (MQ=255)   gCACGACGTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAAc                                                                                                                                                >  2:871886/1‑151 (MQ=255)   gCACGACGTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAAc                                                                                                                                                <  2:1288244/151‑1 (MQ=255)    cACGACGTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACg                                                                                                                                               >  2:292968/1‑151 (MQ=255)      cgacgTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCt                                                                                                                                             <  2:1016027/151‑1 (MQ=255)        acgTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTac                                                                                                                                           >  2:66913/1‑151 (MQ=255)         cgTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTacg                                                                                                                                          <  2:1198350/151‑1 (MQ=255)          gTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTacga                                                                                                                                         <  2:792777/151‑1 (MQ=255)            aaCGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTacgacg                                                                                                                                       <  1:507231/151‑1 (MQ=255)            aaCGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTacgac                                                                                                                                        <  2:799736/150‑1 (MQ=255)             aCGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGAcgc                                                                                                                                      <  1:1253761/151‑1 (MQ=255)                 cATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATg                                                                                                                                  <  1:1234333/151‑1 (MQ=255)                 cATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATg                                                                                                                                  >  1:891289/1‑151 (MQ=255)                  aTTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGa                                                                                                                                 <  1:64943/151‑1 (MQ=255)                    tAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGaa                                                                                                                                <  2:1224567/150‑1 (MQ=255)                     aaCGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAAt                                                                                                                               >  1:528436/1‑150 (MQ=255)                     aaCGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATa                                                                                                                              <  2:233271/151‑1 (MQ=255)                      aCGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATaa                                                                                                                             <  2:186336/151‑1 (MQ=255)                         gTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAgg                                                                                                                          <  1:206809/151‑1 (MQ=255)                              gCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCAttt                                                                                                                     >  1:521591/1‑151 (MQ=255)                                aaGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTc                                                                                                                   <  2:1290666/151‑1 (MQ=255)                                aaGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTc                                                                                                                   <  2:71005/151‑1 (MQ=255)                                 aGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCa                                                                                                                  <  1:364580/151‑1 (MQ=255)                                    cTGGTGGCAGCGATTAATACAGCGGTAGGTCTGGGTCTAAACCCGTATTTCGCACCGGCTGTCGATAGCATGGTTCGCGTGAAAGATATCTATATACCGATTGAGAGCAATGGCAAACGCTCCGACGGGATGAATAAAGGCagtgt                                                                                                                    <  1:34983/146‑5 (MQ=255)                                    cTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCa                                                                                                                  >  1:929810/1‑148 (MQ=255)                                         ggCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt                                                                                                           >  2:11786/1‑150 (MQ=255)                                         ggCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTa                                                                                                          <  1:1115627/151‑1 (MQ=255)                                               gATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCaa                                                                                                     >  2:509522/1‑150 (MQ=255)                                                aTTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAAc                                                                                                   <  1:423179/151‑1 (MQ=255)                                                      aCAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTg                                                                                             <  2:1023032/151‑1 (MQ=255)                                                           ggtcggtcTGGGGCTATACCCGGATTACGCAAAGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCCAACATACt                                                                                              >  1:175835/1‑145 (MQ=255)                                                           ggTAGGTCTGGGGCAATACCCGGATTACGCAACGTCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTAGACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACt                                                                                              <  2:175836/145‑1 (MQ=255)                                                                gTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCt                                                                                   <  2:1247809/151‑1 (MQ=255)                                                                   tGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGa                                                                                 >  1:1224431/1‑150 (MQ=255)                                                                    ggggCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGaaa                                                                               <  1:847574/151‑1 (MQ=255)                                                                    ggggCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGaa                                                                                >  1:370457/1‑150 (MQ=255)                                                                             cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTa                                                                       <  2:917539/150‑1 (MQ=255)                                                                                ggATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGaa                                                                   <  2:697288/151‑1 (MQ=255)                                                                                ggATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGa                                                                    >  1:583565/1‑150 (MQ=255)                                                                                    tACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTg                                                                >  1:432926/1‑150 (MQ=255)                                                                                     aCGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGa                                                               >  1:53264/1‑150 (MQ=255)                                                                                     aCGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGAt                                                              >  2:158453/1‑151 (MQ=255)                                                                                      cGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGAt                                                              <  1:685853/150‑1 (MQ=255)                                                                                      cGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATg                                                             >  2:417108/1‑151 (MQ=255)                                                                                      cGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATg                                                             >  1:703343/1‑151 (MQ=255)                                                                                        cAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCa                                                           <  2:232457/151‑1 (MQ=255)                                                                                          aCGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCa                                                           >  2:1015699/1‑149 (MQ=255)                                                                                             gCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtgggg                                                      >  1:394125/1‑151 (MQ=255)                                                                                             gCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtgggg                                                      >  2:984914/1‑151 (MQ=255)                                                                                               tGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtgggga                                                     >  1:72616/1‑150 (MQ=255)                                                                                                 tCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtggggaatt                                                  >  2:388372/1‑151 (MQ=255)                                                                                                  cGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtggggaattg                                                 <  2:662044/151‑1 (MQ=255)                                                                                                  cGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtggggaatt                                                  <  1:545048/150‑1 (MQ=255)                                                                                                   gATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtggggaattgg                                                >  1:507809/1‑151 (MQ=255)                                                                                                   gATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtggggaattgg                                                >  2:799386/1‑151 (MQ=255)                                                                                                    aTAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtggggaattggg                                               <  2:4206/151‑1 (MQ=255)                                                                                                        cATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCAtggggaattggggaag                                           <  2:167800/151‑2 (MQ=255)                                                                                                         aTGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAAtg                                          >  1:411744/1‑151 (MQ=255)                                                                                                           ggTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAAtgtg                                        >  2:1051989/1‑151 (MQ=255)                                                                                                                 cgTGAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCg                                  >  1:39045/1‑151 (MQ=255)                                                                                                                    gAAAGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATc                               >  2:718863/1‑151 (MQ=255)                                                                                                                     aaaGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATcc                              >  1:841147/1‑151 (MQ=255)                                                                                                                      aaGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCa                             <  2:251992/151‑1 (MQ=255)                                                                                                                       aGATATCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAAAAAAACATACTGATGTGATCCTGAGAAAATCGTATGAAGGGATGCATGGGGGATTGGGGAATGAGGCGTCGa                                 >  1:634246/1‑146 (MQ=255)                                                                                                                            tCTTTATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTg                       >  1:534228/1‑151 (MQ=255)                                                                                                                               ttATACCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTg                       <  1:16713/148‑1 (MQ=255)                                                                                                                                tataCCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTc                    <  1:477854/150‑1 (MQ=255)                                                                                                                                 ataCCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCg                   >  2:517066/1‑150 (MQ=255)                                                                                                                                  taCCGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGa                  >  2:682205/1‑150 (MQ=255)                                                                                                                                     cGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGc              >  1:291673/1‑151 (MQ=255)                                                                                                                                     cGATTGAGAGCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGc              >  1:507816/1‑151 (MQ=255)                                                                                                                                             agCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGa      <  1:739591/151‑1 (MQ=255)                                                                                                                                             agCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGa      <  1:133155/151‑1 (MQ=255)                                                                                                                                              gCAATGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTg       >  1:1052939/1‑149 (MQ=255)                                                                                                                                                aaTGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAgg    >  2:719426/1‑150 (MQ=255)                                                                                                                                                 aTGTCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGaa  <  2:830733/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGGCACGACGTAACGCCATTAACGGTTGTGCAAGCCTGGGGGCAGCGATTAATACAGCGGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAA  >  NC_000913/2903088‑2903382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑