Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,946,466	T→C	H238R (CAC→CGC)	mltA ←	membrane‑bound lytic murein transglycosylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,946,466	0	T	C	100.0%	289.8 / NA	77	H238R (CAC→CGC)	mltA	membrane‑bound lytic murein transglycosylase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/45);  total (32/45)

GGAATAATGGAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATC  >  NC_000913/2946322‑2946608                                                                                                                                                 |                                                                                                                                               ggAATAATGGAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGttt                                                                                                                                          >  1:1070542/1‑151 (MQ=255)    ataatGGAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGc                                                                                                                                       <  2:925886/151‑1 (MQ=255)      aatGGAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGccc                                                                                                                                     <  1:50207/151‑1 (MQ=255)         ggAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCa                                                                                                                                   <  2:300277/150‑1 (MQ=255)         ggAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCa                                                                                                                                   <  2:661292/150‑1 (MQ=255)                 cAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAAt                                                                                                                          <  1:979743/151‑1 (MQ=255)                  agagGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATc                                                                                                                         <  1:1203551/151‑1 (MQ=255)                         aCTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGc                                                                                                                   >  2:504271/1‑150 (MQ=255)                         aCTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCa                                                                                                                  <  1:1124635/151‑1 (MQ=255)                            gACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCAt                                                                                                                 <  2:299129/149‑1 (MQ=255)                            gACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCAt                                                                                                                 <  1:349256/149‑1 (MQ=255)                              cgcgcgACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGAc                                                                                                             >  1:31077/1‑151 (MQ=255)                               gcgcgACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACa                                                                                                            <  1:11295/151‑1 (MQ=255)                                   gACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACTGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCTCCAGTGACGAATCGCCTGCATCGACATAtc                                                                                                        <  1:467990/151‑1 (MQ=255)                                   gACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATAtc                                                                                                        >  2:625250/1‑151 (MQ=255)                                         ccaGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCttt                                                                                                  >  2:195905/1‑151 (MQ=255)                                         ccaGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCttt                                                                                                  <  1:1269393/151‑1 (MQ=255)                                         ccaGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCttt                                                                                                  <  1:421117/151‑1 (MQ=255)                                          caGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCtttt                                                                                                 >  1:939749/1‑151 (MQ=255)                                          caGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCtttt                                                                                                 <  2:1258124/151‑1 (MQ=255)                                            gCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCtttttt                                                                                               <  2:1271999/151‑1 (MQ=255)                                            gCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCtttttt                                                                                               <  1:52865/151‑1 (MQ=255)                                              ggCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCa                                                                                             <  2:495681/151‑1 (MQ=255)                                                  ccGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACtt                                                                                          <  1:800908/150‑1 (MQ=255)                                                      aCTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGcc                                                                                      >  1:466966/1‑150 (MQ=255)                                                         tGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCAc                                                                                    <  2:827376/149‑1 (MQ=255)                                                           tcccTTTCACCGGGGCAATAGATTGCGGCTTAAAGAAGCCGAAAGACGGGTTCTGTTCCAGGAGATCGAGTACCTCGGCGTCACTTCGAGTTTCGCCCCAGTGACGAATCGCCTGCAACGGCATATCTTCTTTTCTCACTTCGCCACg                                                                                   >  1:194536/2‑148 (MQ=255)                                                           ccccTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTATTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTc                                                                                <  2:704029/151‑1 (MQ=255)                                                            cccTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCg                                                                               >  1:855854/1‑151 (MQ=255)                                                                ttCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGAt                                                                             <  2:1075250/149‑1 (MQ=255)                                                                ttCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGAt                                                                             <  2:827392/149‑1 (MQ=255)                                                                  cACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAg                                                                          <  2:327260/150‑1 (MQ=255)                                                                   aCCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGc                                                                         >  1:399170/1‑150 (MQ=255)                                                                    ccGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCAc                                                                       >  1:55371/1‑151 (MQ=255)                                                                        aGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTa                                                                   <  2:224626/151‑1 (MQ=255)                                                                           aaaaGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTAcc                                                                 >  2:1063376/1‑150 (MQ=255)                                                                              aGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAAtg                                                             >  2:122140/1‑151 (MQ=255)                                                                              aGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAAtg                                                             <  1:602360/151‑1 (MQ=255)                                                                                aTTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAAtgct                                                           <  1:1069612/151‑1 (MQ=255)                                                                                aTTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAAtgct                                                           <  2:789319/151‑1 (MQ=255)                                                                                  tGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAAtgctgc                                                         >  1:456837/1‑151 (MQ=255)                                                                                  tGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAAtgctg                                                          <  2:189280/150‑1 (MQ=255)                                                                                   gCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTGCCAAtgctgc                                                         <  1:200569/150‑1 (MQ=255)                                                                                    cGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCg                                                        >  2:856614/1‑150 (MQ=255)                                                                                     ggTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGAt                                                      <  2:268115/151‑1 (MQ=255)                                                                                     ggTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGAt                                                      <  1:1212905/151‑1 (MQ=255)                                                                                      gTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGAt                                                      >  2:618022/1‑150 (MQ=255)                                                                                         tAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGc                                                  >  2:1273671/1‑151 (MQ=255)                                                                                          aaaGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGc                                                  >  1:266378/1‑150 (MQ=255)                                                                                            agaagaCGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATg                                               >  1:1124368/1‑151 (MQ=255)                                                                                             gaagaCGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGa                                              <  2:114547/151‑1 (MQ=255)                                                                                              aagaCGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGAc                                             >  2:1005991/1‑151 (MQ=255)                                                                                                 aCGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCg                                           >  1:115826/1‑150 (MQ=255)                                                                                                  cGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGtt                                         <  2:950533/151‑1 (MQ=255)                                                                                                     aaGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCTCCTTACCAATGCTGCGATAGGCATGACCGtttt                                       <  1:1103210/150‑1 (MQ=255)                                                                                                     aaGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGttttt                                      >  1:940055/1‑151 (MQ=255)                                                                                                       gACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTcc                                    >  1:45513/1‑151 (MQ=255)                                                                                                       gACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTcc                                    >  2:908983/1‑151 (MQ=255)                                                                                                         ggggCTCTGTTCCAGCAGCACGCGAACCTCGGCTCCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTccc                                   >  1:434673/2‑150 (MQ=255)                                                                                                          gggTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTg                                 >  2:56987/1‑151 (MQ=255)                                                                                                           ggTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGc                                <  1:533064/151‑1 (MQ=255)                                                                                                            gTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCa                               <  1:1234325/151‑1 (MQ=255)                                                                                                              tCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCAt                              >  2:1082546/1‑150 (MQ=255)                                                                                                                tGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGc                           >  1:1158759/1‑151 (MQ=255)                                                                                                                    ccAGCAGCTCGCGAGCCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTg                         <  1:179584/149‑1 (MQ=255)                                                                                                                        cagcTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGGGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGaaaaa                    <  1:748295/150‑1 (MQ=255)                                                                                                                         atcTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGt                  >  2:750481/3‑151 (MQ=255)                                                                                                                          gcTCGCGAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGtt                 <  1:1095270/151‑1 (MQ=255)                                                                                                                             cgcgAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTaa               <  1:1046469/150‑1 (MQ=255)                                                                                                                             cgcgAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTCCCCTGCATAGCTGAAAAAGTTa                <  1:984034/149‑1 (MQ=255)                                                                                                                              gcgAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGc             >  2:436414/1‑151 (MQ=255)                                                                                                                               cgAACCTCGGCTTCATTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCCACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCCTGACCGTTTTTCCCTGCATCGCTGAAAAAGTTa                <  1:752677/147‑1 (MQ=255)                                                                                                                                gAACCTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGATCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCgg           >  2:660860/1‑151 (MQ=255)                                                                                                                                   ccTCGGCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGGCTGCATCGACATATCTTCATTTTTCACTTCGCCGCGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGaat        <  1:1035008/151‑3 (MQ=255)                                                                                                                                       ggCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCa    <  1:221804/151‑1 (MQ=255)                                                                                                                                        gCTTCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCAt   >  1:934170/1‑151 (MQ=255)                                                                                                                                           tCACTGCGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATc  <  1:367126/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                               GGAATAATGGAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATC  >  NC_000913/2946322‑2946608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑