Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	350,070	T→C	V20A (GTG→GCG)	prpC →	2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	350,070	0	T	C	100.0%	364.3 / NA	97	V20A (GTG→GCG)	prpC	2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/47);  total (50/47)

AACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACG  >  NC_000913/349926‑350213                                                                                                                                                 |                                                                                                                                                aactgaactgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACt                                                                                                                                           <  1:258956/151‑1 (MQ=255)   ctgaactgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACttt                                                                                                                                         >  1:245194/1‑151 (MQ=255)   ctgaactgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACttt                                                                                                                                         >  1:364668/1‑151 (MQ=255)     gaactgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACttt                                                                                                                                         <  1:377614/149‑1 (MQ=255)     gaactgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCt                                                                                                                                       >  1:265158/1‑151 (MQ=255)      aactgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTg                                                                                                                                      <  1:592670/151‑1 (MQ=255)        ctgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTgg                                                                                                                                     >  1:225289/1‑150 (MQ=255)         tgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCg                                                                                                                                   >  2:1094664/1‑151 (MQ=255)           tAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGtt                                                                                                                                 >  1:957750/1‑151 (MQ=255)           tAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGtt                                                                                                                                 >  2:740457/1‑151 (MQ=255)             ggTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTcc                                                                                                                               <  2:303108/151‑1 (MQ=255)              gTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCcg                                                                                                                              >  2:70416/1‑151 (MQ=255)                cGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCcggc                                                                                                                            <  2:443876/151‑1 (MQ=255)                 ggATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCcggcg                                                                                                                           >  2:851537/1‑151 (MQ=255)                     aaGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGc                                                                                                                        >  1:402143/1‑150 (MQ=255)                      aGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACAGGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCa                                                                                                                       >  1:703683/1‑150 (MQ=255)                       gACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAAt                                                                                                                     >  1:389506/1‑151 (MQ=255)                           cATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACgg                                                                                                                 >  1:1004182/1‑151 (MQ=255)                            aTAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGgc                                                                                                                <  2:326730/151‑1 (MQ=255)                             tAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGgcg                                                                                                               >  1:792401/1‑151 (MQ=255)                             tAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGgcg                                                                                                               >  2:780484/1‑151 (MQ=255)                              aGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGgcg                                                                                                               >  1:884002/1‑150 (MQ=255)                                 gTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGctct                                                                                                           <  2:64553/151‑1 (MQ=255)                                 gTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGctct                                                                                                           <  1:271621/151‑1 (MQ=255)                                  tCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTg                                                                                                          >  1:1113686/1‑151 (MQ=255)                                     cATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCAc                                                                                                       <  2:547783/151‑1 (MQ=255)                                     cATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCAc                                                                                                       >  2:1108525/1‑151 (MQ=255)                                      aTCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCAcc                                                                                                      >  2:1086609/1‑151 (MQ=255)                                      aTCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCAcc                                                                                                      >  2:1072191/1‑151 (MQ=255)                                      aTCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCAcc                                                                                                      >  2:651507/1‑151 (MQ=255)                                        ccGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGt                                                                                                    <  1:325113/151‑1 (MQ=255)                                         cGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTg                                                                                                   <  2:1139796/151‑1 (MQ=255)                                          gACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTgg                                                                                                  <  1:1178432/151‑1 (MQ=255)                                          gACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTg                                                                                                   >  1:1059145/1‑150 (MQ=255)                                           acaacaATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTggg                                                                                                 >  1:195581/1‑151 (MQ=255)                                           acaacaATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTggg                                                                                                 <  2:666376/151‑1 (MQ=255)                                               caaTCTCGACCCTACACATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTa                                                                                               >  1:1283085/1‑149 (MQ=255)                                                 aTCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTaaa                                                                                             >  2:90835/1‑149 (MQ=255)                                                     cGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTgg                                                                                        <  2:239917/150‑1 (MQ=255)                                                     cGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTg                                                                                         <  2:1151008/149‑1 (MQ=255)                                                     cGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGc                                                                                       >  1:676903/1‑151 (MQ=255)                                                     cGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGc                                                                                       <  2:891496/151‑1 (MQ=255)                                                       aCCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCa                                                                                      >  2:1195062/1‑150 (MQ=255)                                                        cccTACAAATGATAACAATGACGAGGACAAGATGAGCGACACAACTATCCTGCAAAACAGTACCCATGTCATTATACCGTAAAAATCTACCGCACTTTCTGGTGTTCCGGCGGGCTATACGCAGCTCTCCACCGAGGGTAAAATTGGCaa                                                                                     >  1:775107/1‑150 (MQ=255)                                                        cccTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAAt                                                                                    >  2:453971/1‑151 (MQ=255)                                                            aCAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGTCAATGAcc                                                                                >  1:618311/1‑151 (MQ=255)                                                            aCAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGa                                                                                  <  1:1055431/149‑1 (MQ=255)                                                              aaaTGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTg                                                                              <  1:553078/151‑1 (MQ=255)                                                              aaaTGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTg                                                                              <  1:219773/151‑1 (MQ=255)                                                                aTGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGc                                                                             >  1:441361/1‑150 (MQ=255)                                                                     aaCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTAcc                                                                       <  2:1157633/151‑1 (MQ=255)                                                                      aCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACcg                                                                      <  2:147931/151‑1 (MQ=255)                                                                        aaTGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACcgcg                                                                    <  1:804206/151‑1 (MQ=255)                                                                        aaTGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACcgcg                                                                    >  1:1156812/1‑151 (MQ=255)                                                                           gACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCt                                                                 <  1:359823/151‑1 (MQ=255)                                                                              gAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACg                                                              >  1:954335/1‑151 (MQ=255)                                                                              gAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACg                                                              >  2:740535/1‑151 (MQ=255)                                                                                ggACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGat                                                            <  2:278028/151‑1 (MQ=255)                                                                                    aacaTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATAtt                                                         <  2:825495/150‑1 (MQ=255)                                                                                    aacaTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTc                                                        <  2:114748/151‑1 (MQ=255)                                                                                       aTGAGAGACACAACGATCCTGCAAAACAGTCCCCATGTCATTAAACCGAAAAAATCTGCGTCACTTTCTGGCGCTCCGGCGGCCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTAAGATATTCtcg                                                     >  1:278076/1‑149 (MQ=255)                                                                                          aGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATc                                                  <  2:171646/151‑1 (MQ=255)                                                                                            cGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTg                                                <  2:389535/151‑1 (MQ=255)                                                                                            cGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTg                                                <  2:987890/151‑1 (MQ=255)                                                                                             gACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTgg                                               <  2:1084834/151‑1 (MQ=255)                                                                                               cacaACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCg                                             <  2:245201/151‑1 (MQ=255)                                                                                                   aCGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGaaa                                          >  1:906009/1‑150 (MQ=255)                                                                                                   aCGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAAc                                         <  2:240618/151‑1 (MQ=255)                                                                                                   aCGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAAc                                         >  1:288224/1‑151 (MQ=255)                                                                                                     gATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACAt                                       >  1:352214/1‑151 (MQ=255)                                                                                                        ccTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                    <  2:351039/151‑1 (MQ=255)                                                                                                        ccTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                    <  1:924863/151‑1 (MQ=255)                                                                                                             aaaaCAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAAtt                               <  2:171651/151‑1 (MQ=255)                                                                                                              aaaCAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCCCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAAttt                              <  1:1108600/151‑1 (MQ=255)                                                                                                              aaaCAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAAttt                              <  2:702591/151‑1 (MQ=255)                                                                                                              aaaCAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAAttt                              <  1:313924/151‑1 (MQ=255)                                                                                                                aCAGTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTg                             >  2:1155357/1‑150 (MQ=255)                                                                                                                   gTACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaaga                         <  2:638754/151‑1 (MQ=255)                                                                                                                    tACCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaaga                         >  1:870111/1‑150 (MQ=255)                                                                                                                     aCCCATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGaagaag                       <  2:72838/151‑1 (MQ=255)                                                                                                                      cccATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCGATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGaagaag                       >  2:712643/1‑150 (MQ=255)                                                                                                                       ccATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTg                     <  2:436943/151‑1 (MQ=255)                                                                                                                       ccATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTg                     <  2:957783/151‑1 (MQ=255)                                                                                                                       ccATGTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTg                     >  1:192602/1‑151 (MQ=255)                                                                                                                           gTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGTAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                 <  2:245545/151‑1 (MQ=255)                                                                                                                           gTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcg                  <  1:1012273/150‑1 (MQ=255)                                                                                                                             cATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCa                <  1:1055202/150‑1 (MQ=255)                                                                                                                             cATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCAt               >  1:538878/1‑151 (MQ=255)                                                                                                                              aTTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATc              >  1:809075/1‑151 (MQ=255)                                                                                                                                 aaaCCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATctgc           >  1:958789/1‑151 (MQ=255)                                                                                                                                 aaaCCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATctgc           >  2:793108/1‑151 (MQ=255)                                                                                                                                  aaCCGAAAAAGTCTGCGGCCCTGTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATctg            <  1:112403/149‑1 (MQ=255)                                                                                                                                      gAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATc      >  1:421910/1‑151 (MQ=255)                                                                                                                                         aaaaTCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCAc   >  2:906246/1‑151 (MQ=255)                                                                                                                                          aaaTCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCAc   >  2:480593/1‑150 (MQ=255)                                                                                                                                          aaaTCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACg  <  2:759991/151‑1 (MQ=255)                                                                                                                                          aaaTCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACg  >  1:743470/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                AACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACG  >  NC_000913/349926‑350213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑