Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	452,681	A→G	F289L (TTC→CTC)	ampG ←	muropeptide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	452,681	0	A	G	100.0%	331.3 / NA	87	F289L (TTC→CTC)	ampG	muropeptide transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/56);  total (31/56)

AAAGGCTGATGTGCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAACAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGA  >  NC_000913/452535‑452827                                                                                                                                                   |                                                                                                                                                   aaaGGTTGATGAGCCAACCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAg                                                                                                                                                 <  2:266972/150‑1 (MQ=255) aaaGGCTGATGTGCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCa                                                                                                                                                  <  1:170568/149‑1 (MQ=255)  aaGGCTGATGTGCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTg                                                                                                                                               <  2:137707/151‑1 (MQ=255)   gtgctaatttccccATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCCTGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCCGCAGTGCCCGGAGCAGTGa                                                                                                                                              <  1:22192/140‑1 (MQ=255)        gtgttgCCCACCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCGCCGTGTAAAATGCCGAAAAGCAGCAGTGCCCGGAGCAGTGACAGgc                                                                                                                                         <  1:1200787/147‑1 (MQ=255)        gATGTGCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAgg                                                                                                                                          <  1:433929/150‑1 (MQ=255)         aTGTGCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGgcg                                                                                                                                        >  2:522217/1‑151 (MQ=255)         aTGTGCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGgcg                                                                                                                                        >  1:1261866/1‑151 (MQ=255)         aTGTGCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGgc                                                                                                                                         <  2:1101299/150‑1 (MQ=255)          tgtgCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGgcgc                                                                                                                                       >  1:682163/1‑151 (MQ=255)          tgtgCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGgc                                                                                                                                         <  1:38817/149‑1 (MQ=255)           gtgCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAg                                                                                                                                           <  2:952071/146‑1 (MQ=255)           gtgCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCt                                                                                                                                      >  1:1170953/1‑151 (MQ=255)              cccATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGc                                                                                                                                    <  2:598615/150‑1 (MQ=255)               ccATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGc                                                                                                                                    <  1:1009771/149‑1 (MQ=255)                    ccGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCaa                                                                                                                               <  2:1249689/149‑1 (MQ=255)                           agagGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCAcc                                                                                                                      >  2:874599/1‑151 (MQ=255)                             agGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGt                                                                                                                    <  2:1046602/151‑1 (MQ=255)                              ggTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTa                                                                                                                   <  1:631557/151‑1 (MQ=255)                                 tttCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACa                                                                                                                 >  2:682461/1‑150 (MQ=255)                                   tCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAAt                                                                                                               <  2:69804/150‑1 (MQ=255)                                    cGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATgc                                                                                                             >  2:516230/1‑151 (MQ=255)                                     gAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCGCCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCCCCGTACAATgc                                                                                                             <  1:840403/150‑1 (MQ=255)                                     gAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATgcg                                                                                                            >  1:605047/1‑151 (MQ=255)                                     gAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATgcg                                                                                                            >  1:23555/1‑151 (MQ=255)                                        aaaGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCca                                                                                                         <  1:767181/151‑1 (MQ=255)                                          aGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCcaac                                                                                                       <  2:695540/151‑1 (MQ=255)                                             cGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAAt                                                                                                    <  2:1112019/151‑1 (MQ=255)                                             cGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAAt                                                                                                    >  2:841130/1‑151 (MQ=255)                                              ggCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATg                                                                                                   >  2:103328/1‑151 (MQ=255)                                               gCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTACCCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATgg                                                                                                  <  1:1175189/151‑1 (MQ=255)                                               gCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATgg                                                                                                  >  2:688425/1‑151 (MQ=255)                                               gCTGCGCCCAGGCTGTAGAGATGCCTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCCCCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGGACAATGCGCCAACAATgg                                                                                                  <  1:1108238/151‑1 (MQ=255)                                                 tGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTc                                                                                                >  1:1197981/1‑151 (MQ=255)                                                  gcgcCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGt                                                                                                 <  2:1090637/149‑1 (MQ=255)                                                  gcgcCCATGCGGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAGAATGCCGAAAAGCCGCAGTGCCCGGAGCAGTGACCGGCACTGCATCAAAATCCCACCGTACAATGCGCGAAGAATGGTc                                                                                                <  1:165234/150‑1 (MQ=255)                                                     cccATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCt                                                                                             >  2:1111266/1‑150 (MQ=255)                                                       cATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGt                                                                                          >  1:1280312/1‑151 (MQ=255)                                                          gCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAg                                                                                       <  2:450072/151‑1 (MQ=255)                                                            tGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGcc                                                                                     >  2:664161/1‑151 (MQ=255)                                                             gTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGc                                                                                      <  1:412638/149‑1 (MQ=255)                                                               agagATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCaa                                                                                   <  1:422441/150‑1 (MQ=255)                                                               agagATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCaa                                                                                   <  2:610063/150‑1 (MQ=255)                                                                gagaTGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGc                                                                                 <  1:929606/151‑1 (MQ=255)                                                                    tGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGttt                                                                             >  2:990404/1‑151 (MQ=255)                                                                        tATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTg                                                                           <  1:611003/149‑1 (MQ=255)                                                                         aTCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGtt                                                                         <  2:157734/150‑1 (MQ=255)                                                                             gTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCa                                                                    <  1:765468/151‑1 (MQ=255)                                                                               aaTCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACg                                                                  <  2:554953/151‑1 (MQ=255)                                                                                               tAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCAt                                                  >  2:97501/1‑151 (MQ=255)                                                                                                aaCCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCAt                                                  <  1:97507/150‑1 (MQ=255)                                                                                                aaCCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATc                                                 <  2:811313/151‑1 (MQ=255)                                                                                                 aCCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATc                                                 >  2:271864/1‑150 (MQ=255)                                                                                                   cGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCaa                                               >  1:112618/1‑150 (MQ=255)                                                                                                      cGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAAccc                                           >  2:585576/1‑151 (MQ=255)                                                                                                      cGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAAccc                                           >  2:395277/1‑151 (MQ=255)                                                                                                       gTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCg                                          <  2:634538/151‑1 (MQ=255)                                                                                                       gTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCg                                          <  2:1170990/151‑1 (MQ=255)                                                                                                         tAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGa                                         <  2:394673/150‑1 (MQ=255)                                                                                                          aGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGAcg                                       >  2:934523/1‑151 (MQ=255)                                                                                                          aGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGAc                                        <  1:1638/150‑1 (MQ=255)                                                                                                          aGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGAc                                        <  2:1111472/150‑1 (MQ=255)                                                                                                             cGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGAcgcc                                     <  1:569201/150‑1 (MQ=255)                                                                                                               cACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCcgcg                                  <  2:827969/151‑1 (MQ=255)                                                                                                                    tGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATc                              <  2:370214/150‑1 (MQ=255)                                                                                                                    tGTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATc                              <  2:582928/150‑1 (MQ=255)                                                                                                                     gTAAAATGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCa                             <  1:241223/150‑1 (MQ=255)                                                                                                                         aaTGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAAc                        <  2:605078/151‑1 (MQ=255)                                                                                                                          aTGCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACg                       <  2:515725/151‑1 (MQ=255)                                                                                                                            gCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCCCCCGCATCAAACCCGACGCCGCGAATCAAAAACGTg                     <  2:363000/151‑1 (MQ=255)                                                                                                                            gCCGAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTg                     <  2:1229971/151‑1 (MQ=255)                                                                                                                            gCCGAAAATCAGCAGTGCCCGGAGCAGTGAAAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGCCGCTAGTAAGCCAAGCGCTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCaaaa                          >  1:185526/1‑146 (MQ=255)                                                                                                                               gAAAATCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGtt                  >  1:960402/1‑151 (MQ=255)                                                                                                                                 aaaTCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGt                >  1:184079/1‑151 (MQ=255)                                                                                                                                  aaTCAGCAGTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTc               <  2:199534/151‑1 (MQ=255)                                                                                                                                     cagcagTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAgg            >  1:364873/1‑151 (MQ=255)                                                                                                                                      agcagTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGc           <  2:464887/151‑1 (MQ=255)                                                                                                                                        cagTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTc         <  2:90228/151‑1 (MQ=255)                                                                                                                                         agTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTc         >  2:1135642/1‑150 (MQ=255)                                                                                                                                          gTGCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCAt       <  2:998453/151‑1 (MQ=255)                                                                                                                                            gCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATgg     <  2:222605/151‑1 (MQ=255)                                                                                                                                            gCCCGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATg      <  2:879703/150‑1 (MQ=255)                                                                                                                                              ccGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCg   >  2:465294/1‑151 (MQ=255)                                                                                                                                              ccGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCg   <  2:335426/151‑1 (MQ=255)                                                                                                                                               cGGAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGa  <  1:811948/151‑1 (MQ=255)                                                                                                                                               aggAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCg   >  2:237092/2‑150 (MQ=255)                                                                                                                                                ggAGCAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGa  >  2:909228/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   AAAGGCTGATGTGCCCATCCCGCCACAGAGGTTTTCGAAAAAGACGGCTGCGCCCATGCTGTAGAGATGCTTATCAGTAATCGACAGCAGCCAGTAACCGGCGTTAGACGCACCTTGTAAAATGCCGAAAATCAGCAGTGCCCGGAACAGTGACAGGCGCTGCATCAAAATCCCACCGTACAATGCGCCAACAATGGTCGCTAGTAAGCCAAGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGA  >  NC_000913/452535‑452827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑