| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,097,492 | +G | intergenic (+663/+73) | ycdU → / ← serX | putative inner membrane protein/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,097,488 | 1 | . | G | 91.7% | 57.7 / 2.0 | 24 | intergenic (+659/+77) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (10/12); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01 | |||||||||||
ATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTC‑GGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCT > NC_000913/1097338‑1097630 | ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTGGAACCCCGGTc < 1:198026/151‑1 (MQ=14) cACTGCGTTCACGGTCTGGCCAACTGGGTGGCGCCCGTTGTTGGGGCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTc < 2:683042/150‑1 (MQ=11) cGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTC‑GGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGtttacactcc > 1:370080/1‑143 (MQ=0) cGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTC‑GGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggggggttactcgc > 1:557447/1‑140 (MQ=2) cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAcccccc > 2:898722/1‑149 (MQ=255) aCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGa < 1:1662716/150‑1 (MQ=255) ggTCTGTCCAACTGGCTGTCGCCAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGGGGGGGGGGGTTGAAaccccccaa > 2:1158272/1‑146 (MQ=1) gTCCAACTGGCTGTCGCCAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCCATACgct > 2:214536/1‑147 (MQ=11) gTCCAACTGGCTGTCGCCAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTg < 1:566261/150‑1 (MQ=255) gTCGCCAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATacaca < 2:638150/149‑1 (MQ=255) tCGCCAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑Ct < 2:1194821/150‑1 (MQ=255) cGCCAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑Cttt < 2:687674/151‑1 (MQ=255) cGCCAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGGGGGGGGGGGTCTAACCCCCCGCACCTTGCCGTATACACA‑Cttt > 1:183758/1‑151 (MQ=14) ccAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTCGAAACCCCCATACGTTGCCGTATACACA‑Cctt > 1:1119297/1‑149 (MQ=14) ccAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTcc < 2:96335/151‑1 (MQ=255) ccAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTc < 1:202410/150‑1 (MQ=255) cAGTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCa < 1:1630340/151‑1 (MQ=255) gTTGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGTACCCCCCATACCCTTCCCTATACCCACCTTTccgg > 1:547417/1‑149 (MQ=11) ttGTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTCGGAACCCCCCTACGGTGTCGTATAAACA‑Cttttccgg > 1:1079625/1‑145 (MQ=14) gTCGAGCCCCGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCg < 2:1294028/150‑1 (MQ=255) gagccccGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTTGAACCCCCCATCCCTTGTCGTTTACACC‑Cttt > 1:497951/4‑140 (MQ=14) gagccccGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTCGAAACCCCCATACGTTGCCGTATAAACA‑CTTTTCCGGCggg > 2:1047552/4‑147 (MQ=14) agccccGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTCTAACCCCCCCAAAGCTGGCGTATACAAA‑Atttttcggcggg > 1:1598423/3‑139 (MQ=11) ccccGGTCGGGGGTTTCTAATCCCCCGGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTcc < 2:370108/150‑1 (MQ=255) ccccGGTCGGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCt < 1:1688435/151‑1 (MQ=255) | ATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTC‑GGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCT > NC_000913/1097338‑1097630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |