Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,457,1050AG46.0% 148.7 / 44.5 113I441V (ATC→GTC) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (10/51);  new base G (52/0);  total (62/51)
Fisher's exact test for biased strand distribution p-value = 2.19e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

ATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATG  >  NC_000913/4456956‑4457251
                                                                                                                                                       |                                                                                                                                                  
    tCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCa                                                                                                                                                <  1:1600771/150‑1 (MQ=255)
         ggcaggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGa                                                                                                                                           <  1:1302017/150‑1 (MQ=255)
          gcaggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGaaa                                                                                                                                         <  2:617238/151‑1 (MQ=255)
             ggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAActg                                                                                                                                      <  1:884766/151‑1 (MQ=255)
                  cagaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCATCAaaaa                                                                                                                                         >  1:1690888/1‑143 (MQ=255)
                    gaagtggcagaagCCTGCGTTCAGCTTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATg                                                                                                                               <  2:364256/151‑1 (MQ=255)
                    gaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAAATGCTGGGTg                                                                                                                               >  2:565111/1‑151 (MQ=255)
                    gaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTGGT‑GGGGGACATCAGGAAATGCTgg                                                                                                                                  >  1:1248641/1‑148 (MQ=255)
                    gaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑CTTTGGT‑GGGGTCCATCAGAAAATGCTGGATg                                                                                                                               >  2:635459/1‑151 (MQ=255)
                     aagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTGGT‑GGGGTCCATCAGAAACTGCTGGATgg                                                                                                                              >  1:1576439/1‑151 (MQ=255)
                      agtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAAATGCtgggtggg                                                                                                                             >  2:355341/1‑151 (MQ=255)
                         ggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTg                                                                                                                          <  2:990172/151‑1 (MQ=255)
                            agaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGGCCATCAGAAACTGCTGGATGGGCTgggg                                                                                                                       >  1:690150/1‑149 (MQ=255)
                                gCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGG‑GGGGTCCCTCAGAAAATGCTGGGTGGGCTGGCg                                                                                                                       >  2:1129631/1‑147 (MQ=255)
                                 ccTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCATCAGAAACTGCTGGGTGGGCTGGCgaaga                                                                                                                   >  2:1494350/1‑150 (MQ=255)
                                 ccTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGG‑GGGGTCCATCCGAAACTGCTGGAGGGGCTgg                                                                                                                         >  1:1343298/1‑144 (MQ=255)
                                  cTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGG‑GGGGGCCATCAAAAAATGCTGGATggg                                                                                                                             >  1:1032800/1‑139 (MQ=255)
                                  cTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTGGG‑GGGATCCAACAGAAAATGCTGGATGGGGTGGCggagaa                                                                                                                  >  2:1062516/1‑150 (MQ=255)
                                     cGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCg                                                                                                              <  1:1331734/151‑1 (MQ=255)
                                      gTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCATCAGAAACTGCTGGATGGGGTGGCGAAGAAGGCgg                                                                                                             >  2:932971/1‑151 (MQ=255)
                                       ttCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGG‑GGGGTCCATCAGAAACTGCCGGGTGGGCGGGGGGAGAAGgggg                                                                                                             >  1:1710750/1‑150 (MQ=255)
                                        tCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGa                                                                                                            <  2:692379/150‑1 (MQ=255)
                                          aGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTTCATCAGAAACTGCTGGATGGGCTGGCGGAGAAGGCGGGAg                                                                                                          >  2:343695/1‑150 (MQ=255)
                                          aGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTTGT‑GGGGGTCATCAGAAACTTCGGGGTGGGGCGGGGGAGAAGGCgg                                                                                                             >  1:642346/1‑147 (MQ=255)
                                          aGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTTGT‑GGGGGCCCTCAGAAAATGCTGGATGGGGTGGGgaagaaagggggag                                                                                                          >  1:1016802/1‑142 (MQ=255)
                                               gCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCcgcg                                                                                                     <  2:702729/150‑1 (MQ=255)
                                                  cacTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTTCATCAGAAACTGCTTGGTGGGCTGGCGGAAAAAGCGGAAGGcgcg                                                                                                     >  2:40842/1‑147 (MQ=255)
                                                  cacTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGCTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAACTGCTGGATGGGGTGGCGGAGAAGGCGGAAggc                                                                                                        >  2:923611/1‑142 (MQ=255)
                                                   acTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTTGGG‑GGGATCC‑TCAAAAAATGCCGGGAGGGGCGGCGGAGAAGGGGGGAGACgcgccg                                                                                                  >  1:467227/1‑147 (MQ=255)
                                                      ggAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAAt                                                                                              <  2:925776/150‑1 (MQ=255)
                                                           ggCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCATCAGAAACTGCTGGGTGGGCTGGCGAAGAAGGCGGGAGCCGCGCCGGAATTTGGcc                                                                                        >  2:1699862/1‑151 (MQ=255)
                                                             cGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGG‑GGGGTGCATCAGAAAATGCTGGGTGGGGTGGGGGAGAAGGGGGGAGCCGCGCAGGAATTAGGGCtt                                                                                      >  1:696260/1‑151 (MQ=255)
                                                              gATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTa                                                                                     <  1:1059764/151‑1 (MQ=255)
                                                              gATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTa                                                                                     <  2:1115728/151‑1 (MQ=255)
                                                              gATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTa                                                                                     <  2:196878/151‑1 (MQ=255)
                                                               aTGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAg                                                                                    <  2:640690/151‑1 (MQ=255)
                                                                  tgGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAAATGCTGGGTGGGCTGGGGGAGAAGGCGGGAGCCGCGCAGTAAATCGGCCTTAGcc                                                                                  >  2:646754/1‑150 (MQ=255)
                                                                   ggATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGTGGGGGCCCATC‑GAAAATGGTGGATGGGGGGGCGGAGAAAGGGGAAGCGGGGCCGTAAttgcggcttt                                                                                     >  1:159533/1‑138 (MQ=255)
                                                                    gATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGa                                                                               <  2:1679113/151‑1 (MQ=255)
                                                                    gATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGa                                                                               <  2:1316589/151‑1 (MQ=255)
                                                                       aCGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGatat                                                                            <  1:1406203/151‑1 (MQ=255)
                                                                       aCGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGata                                                                             <  1:591228/150‑1 (MQ=255)
                                                                       aCGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGata                                                                             <  2:921075/150‑1 (MQ=255)
                                                                          cTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTTCATCAGAAAATGCTGGGTGGGGTGGCGAAGAAGGCGGAAGCCGCGGCGTAATTTGGCCTTAGCCAGa                                                                               >  2:805943/1‑145 (MQ=255)
                                                                            ggCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGt                                                                         <  2:967761/149‑1 (MQ=255)
                                                                            ggCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTa                                                                        <  1:872083/150‑1 (MQ=255)
                                                                            ggCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTAt                                                                       <  2:1353350/151‑1 (MQ=255)
                                                                               aGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAAATGCTGGGTGGGGTGGGGAAGAAGGGGGGAGCCGCGCAGGAATTCGGCCTTAGCCAGATATAGTATGCg                                                                    >  2:386657/1‑151 (MQ=255)
                                                                                gATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCg                                                                    <  2:1261707/150‑1 (MQ=255)
                                                                                 atatGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGG‑GGGGTCCAACAGAAAATGCTGGGTGGGGTGGCGAAGAAAGCGGGAGGCGCGCAGGAATTTGGCCTTAGCCAGATATAATATGCg                                                                    >  1:1595737/1‑149 (MQ=255)
                                                                                  tatGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCCTAGCCCGATATAGTATGCGt                                                                   >  2:479255/1‑149 (MQ=255)
                                                                                  tatGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGG‑GGGGTCCATCAGAAACTGCTGGGTGGGGTGGCGAAGAAGGCGGGAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGt                                                                   >  2:575689/1‑149 (MQ=255)
                                                                                   atGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAg                                                                 <  2:1357004/150‑1 (MQ=255)
                                                                                     ggtggtGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGc                                                               <  2:353878/150‑1 (MQ=255)
                                                                                       tggtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGG‑GGGGTCCATCAGAAACTGCTGGATGGGGTGGCGGAAAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCgg                                                            >  1:265347/1‑151 (MQ=255)
                                                                                        ggtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGG‑GGGGTCCATCAGAAAATGCTGGATGGGGTGGCGGAGAAGGCGGGAGCCGCGCAGGAATTCTGCCTTAGCCAGATATAGTATGCGGAGGCCgg                                                            >  1:1339701/1‑150 (MQ=255)
                                                                                        ggtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCgg                                                            <  1:1336153/150‑1 (MQ=255)
                                                                                        ggtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGa                                                           <  1:598505/151‑1 (MQ=255)
                                                                                           gAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCCTCAGAAACTGCTGGGTGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATa                                                         >  2:1626046/1‑150 (MQ=255)
                                                                                            aaaaCCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAAATTCTGGATGGGGTGGGGGAGGAGGCGGGAGGCGCGCGGTAATTCGGCCTTTGGCAGATATAGGATGCGGAGGCCGGGTa                                                         >  1:1256310/1‑149 (MQ=255)
                                                                                            aaaaCCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAg                                                       <  2:1084614/151‑1 (MQ=255)
                                                                                                ccGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGt                                                   <  1:1507245/151‑1 (MQ=255)
                                                                                                 cGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAAATGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGGTAAGGCggt                                                  >  2:1128551/1‑149 (MQ=255)
                                                                                                 cGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGtt                                                  <  2:1130119/151‑1 (MQ=255)
                                                                                                  gCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAAATGCTGGGTGGGGTGGCGGAGAAAGCGGGAGCCGCGCAGTAATTCGGCCTTAGCCCGATATAGTATGCGTAGGCCGGGTAAGGCGtt                                                  >  1:20252/1‑150 (MQ=255)
                                                                                                  gCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAAATGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGGTAAGGCggt                                                  >  2:462776/1‑148 (MQ=255)
                                                                                                   cTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAAACGCTGGATTGGCTGGCGAAGAAAGCGGAAGCCGCGCAGTAATTTGGCCTTAGCCCGATATAATATGCGTAGGCCGGATAAAGCggt                                                  >  2:314503/1‑147 (MQ=255)
                                                                                                    tCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCATCAGAAACTGCTGGGTGGGGTGGCGGAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCCGATATAGTATGCGTAGGCCGGGTAAGGCggt                                                  >  2:528877/1‑146 (MQ=255)
                                                                                                    tCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGG‑GGGGTCCATCAGAAACTGCTGGATGGGGTGGCGGAGAAAGCGGAAGCCCCGCAGTAATTCGGCCTTAGCCCGATATAGTATGCGTAGGCCGGATAAGGCGTTCAc                                               >  1:474431/1‑151 (MQ=255)
                                                                                                     cAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCAcg                                              <  1:212478/151‑1 (MQ=255)
                                                                                                      aGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAACTGCTGGATGGGGTGGGGGAGAAGGCGGGAGCCGCGCAGGAATTTCGGCTTAGCCCGATATAGTATGCGTAGGCCGGGGAAGGCGGTCAcgc                                             >  1:1473611/1‑151 (MQ=255)
                                                                                                       gCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCATCAGAAAATGGTGGATGGGGTGGGGAAGAAAGCGGGAGCCGCGCAGGAAATCGGCCTTAGCCAGATATAGTATGGGTAGGCCGGGTAAGGGGTTCAcgcc                                            >  2:1403016/1‑151 (MQ=255)
                                                                                                        ccTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCAcgccg                                           <  1:1133385/151‑1 (MQ=255)
                                                                                                         cTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAAATGCTGGATGGGCTGGGGAAGAAAGCGGGAGCCGCGCAGTAATTCGGCCTTAGCCAGAAATAGTATGCGTAGGCCGGGTAAGGGGGTCAcgcc                                            >  2:713533/1‑149 (MQ=255)
                                                                                                          tGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCCTCAGAAACTGCTGGGTGGGCTGGCGAAGAAGGCGGGAGCCGCGCAGTAAATCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGGTAAGGCGGTCAcgccgcc                                         >  2:1278877/1‑150 (MQ=255)
                                                                                                           ggCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCa                                         <  1:932903/150‑1 (MQ=255)
                                                                                                           ggCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCa                                         <  1:409842/150‑1 (MQ=255)
                                                                                                           ggCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCAt                                        <  1:392974/151‑1 (MQ=255)
                                                                                                             cGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCATCAAAAAATGCTGGGTGGGGTGGCGGAGAAGGCGGGAGCCGCGCAGGAATTTGGCCTTAGCCCGATATAATATGCGTAGGCCGGGTAAGGCGGTCCCGCCGCATcc                                      >  2:924843/1‑151 (MQ=255)
                                                                                                              gACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAACTGCTGGATGGGCTGGCGGAGAAGGCGGGAGCCGCGCAGGAATTCGGCCTTAGCCAGATATAGTATGCGGAGGCCGGGTAAGGCGGTCACGCCGCATcc                                      >  1:463923/1‑150 (MQ=255)
                                                                                                               aCCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCATCAGAAACTGCTGGGTGGGGTGGCGGAGAAAGCGGGAGCCGCGCAGTAAATCGGCCTTAGCCAGATATAGTATGCGTAAGCCGGGTAAAGCGGTCACGCCCCATCCg                                     >  1:1422244/1‑150 (MQ=255)
                                                                                                               aCCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                    <  2:1664984/151‑1 (MQ=255)
                                                                                                                 cATATTCTGGTGATGAGCAACGGCGG‑TTTTTGT‑GGGGTCCATCAGAAACCGCTGGATGGGCTGGCGAAGAAAGCGGAAGCCGCGCAGGAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGGTAAGGCGGTCACGCCGCCTCCGGc                                   >  1:299033/1‑150 (MQ=255)
                                                                                                                  atatTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                 <  1:229579/151‑1 (MQ=255)
                                                                                                                    atTCTGGTGATGAGCAACGGCGG‑TTTTTGG‑GGGATCCATCAGAAAATGCTGGGTGGGGTGGCGAAAAAAGCGGGAGCCGCGCCGCAATTCGGCCTTTGCCAGATATAATATTCGTAGGCCGGGTAAGGCGTTCACGCCGCCTCCCGCCttt                               >  1:1181258/1‑151 (MQ=255)
                                                                                                                    atTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGAGTCAACACGAACAGCTGGGTGGGCGGGGGAAAAAAGAGGAAAACGCACAGTAAATTGGAGTTAGGCAAAGATAATATGCGTAAGACGGCTAAGGGGTTTTACCCTAATCCGGCAtt                                >  1:1511529/1‑150 (MQ=255)
                                                                                                                       cTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAACTGCTGGATGGGCTGGCGGAGAAAGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAttt                               >  1:92922/1‑148 (MQ=255)
                                                                                                                            gatgaGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATa                        <  1:3648/150‑1 (MQ=255)
                                                                                                                              tgaGCAACGGCGG‑TTTTGGT‑GGGGTCCATCAGAAACTGCTGGGTGGGGTGGCGAAGAAAGCGGGAGCCGCGCCGTAATTTGGCCTTAGCCAGATATAGTATGCGTAGGCCGGGTAAGGCGTTCACGCCGCATCCCGCATTTTAGCATAGTg                     >  1:848356/1‑151 (MQ=255)
                                                                                                                                  cAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTg                 <  2:1248738/151‑1 (MQ=255)
                                                                                                                                        cgg‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGa           <  2:193513/151‑1 (MQ=255)
                                                                                                                                         gg‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGAc          >  2:741941/1‑151 (MQ=255)
                                                                                                                                          g‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACg         >  2:779458/1‑151 (MQ=255)
                                                                                                                                            ttttGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACg         <  1:1494249/150‑1 (MQ=255)
                                                                                                                                              ttGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCt       <  2:1087408/150‑1 (MQ=255)
                                                                                                                                               tggt‑ggGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCtt      <  1:1713962/150‑1 (MQ=255)
                                                                                                                                               tggt‑ggGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTg     >  2:588607/1‑151 (MQ=255)
                                                                                                                                                 gt‑ggGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGAt   >  1:1579822/1‑151 (MQ=255)
                                                                                                                                                 gt‑ggGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGAt   <  2:1343391/151‑1 (MQ=255)
                                                                                                                                                    gggATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATg  <  2:1691003/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
ATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATG  >  NC_000913/4456956‑4457251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑