Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4960TA46.9% 113.7 / 73.0 98intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (45/7);  new base A (0/46);  total (45/53)
Fisher's exact test for biased strand distribution p-value = 8.35e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29346‑29645
                                                                                                                                                       |                                                                                                                                                     
tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                       >  2:524730/1‑151 (MQ=255)
 ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                       >  1:1017803/1‑150 (MQ=255)
   aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                     >  2:1412724/1‑150 (MQ=255)
        aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                                <  2:1425009/150‑1 (MQ=255)
        aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATc                                                                                                                                               >  2:637852/1‑151 (MQ=255)
          aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                             >  2:863088/1‑151 (MQ=255)
           ttttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                            >  1:510442/1‑151 (MQ=255)
            tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                            <  2:1041398/150‑1 (MQ=255)
            tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGc                                                                                                                                           >  1:1515187/1‑151 (MQ=255)
             ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                          >  1:868803/1‑151 (MQ=255)
             ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGc                                                                                                                                           <  1:1357288/150‑1 (MQ=255)
              tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                          <  2:1650336/150‑1 (MQ=255)
                   aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                      >  2:1164355/1‑149 (MQ=255)
                         tCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACt                                                                                                                              >  2:713788/1‑151 (MQ=255)
                         tCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACt                                                                                                                              >  2:626744/1‑151 (MQ=255)
                          ccttttttttCTGCTCGTTTTTATTTCATGAAATCTTCTTGCTGCGCAAGCTTTTCCAAGAACGGGTTAGATGATTTTTTTGCCGCTTA‑ATGCCTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACt                                                                                                                              <  2:518752/146‑1 (MQ=255)
                           cACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                            >  2:851803/1‑151 (MQ=255)
                           cACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                            >  2:628556/1‑151 (MQ=255)
                              ttttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                           >  2:429943/1‑149 (MQ=255)
                              ttttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                           >  2:628223/1‑149 (MQ=255)
                                ttttCTGCTTTTTTTTATTTCATGCATTTTTTTTGCTGCGAAAGCTTTTCCAAAAACAGGTTAGATAATTTTTTTGTCTCTTA‑ATGCCTAAAAAACATGCATGAGCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAgc                                                                                                                        <  1:1408543/150‑1 (MQ=255)
                                 tttCTGTTTTTTTTTTTTTCATGAATTTTTCTTGCGGCGCAGGCTTTTCCAAAAACAGGTTAAATATTTTTTTTGTCGTTTA‑AGGCTTAAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                       <  1:1579212/150‑1 (MQ=255)
                                 tttCTGCTGTTTTTTTTTTCATCAATTCTTCTTGCGCCGAAGGTGTTTCCAAGAACAGGTTAAAAGATTTTTTTGTCCCTAA‑TTCCTTGTAAAACCGGCATGAGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                       <  1:415557/150‑1 (MQ=255)
                                  ttCTGCTCTTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCTTTTCCCAAAACAGTTTAGATGATTTTTTTGTCGTTTA‑ATGCTTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                                      <  2:1242316/150‑1 (MQ=255)
                                  ttCTCCTCTTTTTTTTTTAATGCAATCTTCTTGCTGCACAAGCTTTTCCCAGAACGGGTTAGATTTTTTTTTTGCCGTTTA‑ATGTCTTAAAAAAATGCATGGCCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                                      <  2:756612/150‑1 (MQ=255)
                                    cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                    >  1:934785/1‑150 (MQ=255)
                                         cGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                              >  2:465504/1‑151 (MQ=255)
                                           ttttttttTCATACATTCTTCTTGCTGCACAAGCTTTTCCAAAAACAGGTTAGATGATTTTTTTGCCGCTTA‑ATGCCTTAAAAACTTGCATGAGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                                  <  1:645529/145‑1 (MQ=255)
                                           tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                            >  1:866341/1‑151 (MQ=255)
                                            ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                            >  2:1008946/1‑150 (MQ=255)
                                              ttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                            >  1:340252/1‑148 (MQ=255)
                                                 tttCATGCATTCTTCTTGCTGCGAAAGCTTTTCCAAAAACAGTTTAAATGATTTTTTTGTCGTTAA‑ATCCCTGAAAAACAGGCATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                       <  1:593560/150‑1 (MQ=255)
                                                  ttCATGAATTTTTTTTCCTGCCAAAGCTTTTCCCAAAACAGTTTAATTTTTTTTTTTGTCCTTTA‑ATCCCTGTAAAACTTGCATGGGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                                      <  1:69775/150‑1 (MQ=255)
                                                   tCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgcc                                                                                                    >  1:1417720/1‑151 (MQ=255)
                                                     aTGCATTCTTCTTGCGCCGCAAGTTTTTCCCAAAACGGTTAAGATGTTTTTTTTGCCGCTTA‑ATCCCTTAAAAACAGGAATGACCCAAAAAAAAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgc                                                                                                  <  2:1032110/151‑1 (MQ=255)
                                                     aTGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgc                                                                                                  >  2:1287757/1‑151 (MQ=255)
                                                       gaattcttctTGCTGCGCAAGCGTTTCCAAGAACAGTTTAGATGTTTTTTTTGTCGCTTA‑ATGCCTGAAAAACATGAATGGCCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                                 <  2:378455/146‑1 (MQ=255)
                                                       gCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                                >  2:758234/1‑151 (MQ=255)
                                                         aaTCTTCTGGCTGCGCAAGCGTTTCCAAGAACAGGTTAGATGATTTTTTTGTCGTTTA‑ATGCCTTAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                                 <  2:1374696/148‑1 (MQ=255)
                                                           tcttctTGCTGCGCAAGCGTTTTCAAGAACAGGTTAAATGATTTTTTTGTCGCTTA‑ATCCTTAAAAAACATGAATGACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                                 <  1:1310747/146‑1 (MQ=255)
                                                           tcttctTGCTGCGAAAGCTTTTACAAAAACGGGTTAAATAATTTTTTTGTCTCTTA‑ATCCCTAAAAAACATGCATGAGCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                                 <  1:370228/146‑1 (MQ=255)
                                                             tttttGCTGCGAAAGCTTTTCCCAAAACAGTTTAGATGTTTTTTTTGTCGTTTA‑ATGCCTAAAAAACATGCATGACCCAAAAAATAAAAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                             <  2:421628/145‑1 (MQ=255)
                                                                ttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                          >  2:1678983/1‑148 (MQ=255)
                                                                ttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                          >  2:1088926/1‑148 (MQ=255)
                                                                 tgctgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                       >  1:857683/1‑150 (MQ=255)
                                                                  gctgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                      >  2:1604586/1‑150 (MQ=255)
                                                                    tgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                   >  2:1400280/1‑151 (MQ=255)
                                                                    tgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                   >  1:1254555/1‑151 (MQ=255)
                                                                     gcccagttttttgCCAGAACAGGATATTTTATTTTTTTGTCCATAA‑ATTCCTGAAAAATGTGCAGGAACCAAAAAATAATATAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                                     <  1:1381676/139‑1 (MQ=255)
                                                                      ccaaactttttcCCAGAAAAGTTAAGAGAATTTTTTTATC‑CTTACATCCCTGAAAAACCGGCACAACCCCAAAAATAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                             <  1:713202/131‑1 (MQ=255)
                                                                       gcAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                                >  2:298367/1‑151 (MQ=255)
                                                                          aGCTTTTTCAAAAACAGGTTAGATGTTTTTTTTGTCTTTTA‑ATGCGTGAAAACCATGAATGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                                  <  2:1485870/146‑1 (MQ=255)
                                                                                ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                        >  1:8481/1‑150 (MQ=255)
                                                                                   cAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                        >  1:1536572/1‑147 (MQ=255)
                                                                                      aaCAGGTTAGATGATTTTTTTGTCGTTTA‑ATCCCTGAAAAACATCCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                                  <  2:1201233/150‑1 (MQ=255)
                                                                                                    acTTTTTGACGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                   >  1:169759/2‑151 (MQ=255)
                                                                                                      ttttttccttttA‑ATGCCTTAAAAACTTCCTTGACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                                  <  2:340276/140‑1 (MQ=255)
                                                                                                       ttttGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                                >  2:76650/1‑151 (MQ=255)
                                                                                                             ccttaa‑aTGCTTGAAAAACATGCATGAGCCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                           <  2:57062/145‑1 (MQ=255)
                                                                                                             cGCTTA‑ACGCCTATAAAACATGCATGAGCCAAAAAATAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                           <  1:863020/150‑1 (MQ=255)
                                                                                                                ttA‑ATGCTTAAAAAACATGCATGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                         <  2:1017880/149‑1 (MQ=255)
                                                                                                                ttA‑AGGCCTGTAAAACTTGAATGAGCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                           <  1:1188289/147‑1 (MQ=255)
                                                                                                                  a‑aTGCCTGAAAAACATGCAGGACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                          <  1:1400188/146‑1 (MQ=255)
                                                                                                                   aatccttGTAAAACATGCAGGTGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                         <  1:1287664/141‑1 (MQ=255)
                                                                                                                       cttgaaaaaaaTGCAGGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                    <  2:1187093/143‑1 (MQ=255)
                                                                                                                          gTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                               >  2:830805/1‑150 (MQ=255)
                                                                                                                            aaaaCATGCTTGACCCAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTTGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                             <  1:389665/150‑1 (MQ=255)
                                                                                                                            aaaaCATGCATGCGCAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                              <  2:510493/149‑1 (MQ=255)
                                                                                                                            aaaaCATGCATGAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                              <  2:1192808/149‑1 (MQ=255)
                                                                                                                            aaaaCATGCATGAGCAAAAAAAAAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                                <  2:925403/147‑1 (MQ=255)
                                                                                                                            aaaaCATCAATAACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                                 <  1:911199/146‑1 (MQ=255)
                                                                                                                             aaaCATGAATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                           <  2:472597/151‑1 (MQ=255)
                                                                                                                                catgcatgACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                         <  1:1406839/150‑1 (MQ=255)
                                                                                                                                 atgcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                         >  2:350917/1‑149 (MQ=255)
                                                                                                                                  tgcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                      >  2:302044/1‑151 (MQ=255)
                                                                                                                                    catgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                     >  1:408390/1‑150 (MQ=255)
                                                                                                                                     atgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                     >  1:773174/1‑149 (MQ=255)
                                                                                                                                     aggacccAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGATTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                      <  1:1345188/143‑1 (MQ=255)
                                                                                                                                      tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt                  >  2:854796/1‑151 (MQ=255)
                                                                                                                                       gcaaaacaAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa                        <  1:403978/139‑1 (MQ=255)
                                                                                                                                        aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg                 >  1:18231/1‑150 (MQ=255)
                                                                                                                                          ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat              >  2:1288901/1‑151 (MQ=255)
                                                                                                                                          ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat              >  2:1187509/1‑151 (MQ=255)
                                                                                                                                          ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat              >  1:71382/1‑151 (MQ=255)
                                                                                                                                            aaaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa               <  2:209846/146‑1 (MQ=255)
                                                                                                                                            aaaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata             <  1:877720/148‑1 (MQ=255)
                                                                                                                                            aaaaaaTAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt                  <  1:626687/143‑1 (MQ=255)
                                                                                                                                            aaaaaaTAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat              <  1:1447730/147‑1 (MQ=255)
                                                                                                                                             aaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata             <  2:798168/148‑1 (MQ=255)
                                                                                                                                             aaaaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt           <  2:1403052/150‑1 (MQ=255)
                                                                                                                                              aaaaaaaaaaaaaaaaCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa               <  1:425548/146‑1 (MQ=255)
                                                                                                                                              aaaaTAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc          <  1:1412632/151‑1 (MQ=255)
                                                                                                                                              aaaaTAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa               <  1:628498/146‑1 (MQ=255)
                                                                                                                                               aaaaaaTAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct         <  2:1144473/151‑1 (MQ=255)
                                                                                                                                               aaaaaaTAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct         <  1:1599430/151‑1 (MQ=255)
                                                                                                                                                aataatATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc        >  2:790601/1‑151 (MQ=255)
                                                                                                                                                aataatAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc        <  1:87038/151‑1 (MQ=255)
                                                                                                                                                 ataataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct       >  1:1393801/1‑151 (MQ=255)
                                                                                                                                                      ataAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAgg  >  1:945395/1‑151 (MQ=255)
                                                                                                                                                      ataAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAgg  >  1:517686/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTA‑ATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29346‑29645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑