Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913213,3410AG43.0% 160.6 / 25.3 101G337G (GGA→GGG) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (9/48);  new base G (43/0);  total (53/48)
Fisher's exact test for biased strand distribution p-value = 1.13e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCC  >  NC_000913/213191‑213490
                                                                                                                                                      |                                                                                                                                                       
aGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGa                                                                                                                                                         <  2:829592/151‑1 (MQ=255)
   tGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAg                                                                                                                                                       >  1:1454675/1‑150 (MQ=255)
    gCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAgtg                                                                                                                                                     >  1:851827/1‑151 (MQ=255)
      cTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAgt                                                                                                                                                      >  2:1037098/1‑148 (MQ=255)
        cACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAggg                                                                                                                                                     >  1:1074434/1‑145 (MQ=255)
           ccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTaaa                                                                                                                                                 >  1:699894/1‑146 (MQ=255)
             tGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑Ct                                                                                                                                             <  1:1077596/149‑1 (MQ=255)
                   cGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCgg                                                                                                                                     <  2:790958/151‑1 (MQ=255)
                      ttGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGa                                                                                                                                   <  1:758515/150‑1 (MQ=255)
                        gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAG‑CTTAAAGCgggggg                                                                                                                                 >  1:449038/1‑150 (MQ=255)
                          gcgcGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGa                                                                                                                              <  2:209484/151‑1 (MQ=255)
                          gcgcGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGa                                                                                                                              <  2:1016691/151‑1 (MQ=255)
                          gcgcGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGa                                                                                                                              <  1:216255/151‑1 (MQ=255)
                           cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGat                                                                                                                             <  2:705258/151‑1 (MQ=255)
                            gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGGAAAA‑CTTTATGCgggggg                                                                                                                                 >  1:1509833/1‑146 (MQ=255)
                             cgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGatat                                                                                                                           <  1:838273/151‑1 (MQ=255)
                              gTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAG‑CTTAATGCGGGAGGGGATAtt                                                                                                                          >  2:1161597/1‑151 (MQ=255)
                              gTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATAtt                                                                                                                          <  1:696709/151‑1 (MQ=255)
                               tttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAG‑CTTAATGCGGGGGGCGGTAtt                                                                                                                          >  2:268348/1‑150 (MQ=255)
                                 tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGGGTGTACAG‑CTTAATGCGGGGGGCGgtat                                                                                                                           >  1:105965/1‑147 (MQ=255)
                                 tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCg                                                                                                                        <  1:1243214/150‑1 (MQ=255)
                                  gAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTGCAG‑CTTTAAGCGGGGGGCGGAATTCTcc                                                                                                                      >  1:321986/1‑151 (MQ=255)
                                  gAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGc                                                                                                                       <  1:927344/150‑1 (MQ=255)
                                   aaaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGCAGAGGTCAT‑CTTAATTACGGAGGCGGTAtt                                                                                                                          >  2:1074354/1‑146 (MQ=255)
                                    aaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCt                                                                                                                    <  2:1643564/151‑1 (MQ=255)
                                       ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAG‑CTTAATGCGGGGGGGGATATTTGCCCTcc                                                                                                                  >  2:1221113/1‑150 (MQ=255)
                                       ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTcc                                                                                                                  <  1:1013972/150‑1 (MQ=255)
                                       ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGTAAAG‑CTTCATAAGGGAGGGGAGTTTCGCCCTc                                                                                                                   >  2:1617180/1‑149 (MQ=255)
                                         gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGAAAG‑CTTAATTCGGGAGGGGGTATTTGCCCCCcgc                                                                                                                >  2:1045415/1‑150 (MQ=255)
                                          aCGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGt                                                                                                              <  2:1278294/151‑1 (MQ=255)
                                           cGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTg                                                                                                             <  1:1293277/151‑1 (MQ=255)
                                            gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAAGGGACAG‑CTTAAAGCGGGGGGGGGGAATCGcccccc                                                                                                                  >  1:252936/1‑145 (MQ=255)
                                              tATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAG‑CTTAAAGCGGGAGGGGAGATTTGCCCCCcgc                                                                                                                >  2:1268413/1‑145 (MQ=255)
                                                 cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGAc                                                                                                        <  2:900999/150‑1 (MQ=255)
                                                 cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCCGGGAAGGGGACAG‑CTTAATGCGGGGGGCGGTATTTGCCCCCCCCGCGCa                                                                                                           >  1:249505/1‑147 (MQ=255)
                                                  aGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAAAGTTAAGACTTAATCGGGGGGGGGATATTGGCCCTcccggg                                                                                                              >  1:1037628/1‑140 (MQ=255)
                                                     cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAG‑CTTTATGCGGGGGGCGGTATTTGcccccccc                                                                                                                >  1:1160568/1‑136 (MQ=255)
                                                     cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAG‑CTTAATGCGGGGGGCGATATTTGCCCTCCCCGTGCAGa                                                                                                         >  2:1640997/1‑145 (MQ=255)
                                                     cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAG‑CTTTATGCGGGGGGCGGTATTCGCCCTCCGCGTGCAGACGaa                                                                                                     >  2:1498269/1‑149 (MQ=255)
                                                      gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAG‑CTTTATGCGGGGGGGGATATTTGCCCCCCGCGGGCAGACg                                                                                                       >  2:64028/1‑146 (MQ=255)
                                                       cAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAG‑CCTAATGCGGGGGGCGATATTCGCCCTCCGCGTGCAGACGAAACgg                                                                                                 >  2:532482/1‑151 (MQ=255)
                                                        aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAA‑CTTAATTCGGGGGGGGATATTTGCCCCCCCCGGGCAGACGAAGCgg                                                                                                 >  2:770919/1‑150 (MQ=255)
                                                        aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGCGCGGGGGGGAGTACAA‑CTTTATTCGGGAGGCGGTATTCGCCCCCCGCCTGCAGAGGaa                                                                                                     >  2:497040/1‑146 (MQ=255)
                                                           tgtgGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAA‑CCTTATTCGGGGGGGGGAATTTGCCCCCCCCGTGCCGACGaaaggg                                                                                                 >  1:708889/1‑143 (MQ=255)
                                                            gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc                                                                                            <  2:145924/151‑1 (MQ=255)
                                                                  gATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTGCAG‑CGTAATTCGGGGGGGGGTATTTGCCCCCCCCGCGCGGACGAAGCgggcgggggggg                                                                                       >  1:982808/1‑140 (MQ=255)
                                                                   aTTAAATCCGTCACCGGGCAATGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAG‑CTTAATGCGGGAGGCGATATTTGCCCTCCGCGTGCAGACGAAGCGGGCAgcgggc‑ggt                                                                                    >  2:562278/1‑149 (MQ=255)
                                                                        aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAG‑CTTTATTCGGGGGGCGGTATTTTCCCCCCCCCTGCAGACGAAGCGGGCCGCGTGC‑Gttt                                                                                   >  2:240883/1‑147 (MQ=255)
                                                                          ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAgc                                                                             <  1:1581017/151‑1 (MQ=255)
                                                                           cGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAgcg                                                                            <  1:99981/151‑1 (MQ=255)
                                                                              cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCa                                                                         <  2:302017/151‑1 (MQ=255)
                                                                                ccGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAgg                                                                       <  2:229510/151‑1 (MQ=255)
                                                                                 cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAG‑CTTAATGCGGGGGGGGATATTTCCCCCCCGCGGGCCGACGGAGCGGTTCGCGCGG‑GTTTCAAAGCGCCAgg                                                                       >  2:1696227/1‑150 (MQ=255)
                                                                                   ggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGAtt                                                                    <  1:822104/151‑1 (MQ=255)
                                                                                   ggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGAtt                                                                    <  1:1231924/151‑1 (MQ=255)
                                                                                    gCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATtg                                                                   <  1:262381/151‑1 (MQ=255)
                                                                                    gCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATtg                                                                   <  2:597757/151‑1 (MQ=255)
                                                                                    gCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATtg                                                                   <  2:851894/151‑1 (MQ=255)
                                                                                       aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATtgctg                                                                <  2:382261/151‑1 (MQ=255)
                                                                                         gCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCa                                                              <  2:461214/151‑1 (MQ=255)
                                                                                          cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGTGGGAGTGGAACG‑CTTAAAGCTGGGGGAGATATTTGTCCTCCCCGGGCAGACGAAGCGGCGGACGGGC‑GTTTGAAAGCGCCACGAtttcctctt                                                             >  2:801817/1‑144 (MQ=255)
                                                                                           gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCat                                                             <  2:1087299/150‑1 (MQ=255)
                                                                                            aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCatat                                                           <  2:1338198/151‑1 (MQ=255)
                                                                                            aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCGCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCatat                                                           <  2:83181/151‑1 (MQ=255)
                                                                                               aCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAG‑CCTTATGCGGGAGGGGGTATTTGCCCTCCCCGGGCAGACGAAGCGGGCAGGGGGG‑GGTTTAAAACGCCCggggttgtggctatt                                                          >  1:255795/1‑136 (MQ=255)
                                                                                               aCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGt                                                        <  1:1567538/151‑1 (MQ=255)
                                                                                                  ttGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAG‑CTTAAAGCGGGGGGGGATATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGTGC‑GTTTCAAAACGCCAGGATTGCTGCATATTGTCgg                                                     >  2:729218/1‑151 (MQ=255)
                                                                                                  ttGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCCGGGGAGTGTACAG‑CTTAATGCGGGGGGCGGTATTCGCCCTCCCCGTGCAGAAGAAGAGGTCAGCGGGC‑GGTTCAAAACGCCAGGATTGCTTCATATTTTCgg                                                     >  2:1047611/1‑151 (MQ=255)
                                                                                                   tgtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCgg                                                     <  1:183949/150‑1 (MQ=255)
                                                                                                    gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGc                                                   <  2:1037706/151‑1 (MQ=255)
                                                                                                         gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGAAGGGGAAAG‑CTTAATGCGGGCGGCGGTATTTGCCCCCCCCGCGGCGAGGAAGCGGGCAGCCGGC‑GGTTCAAAACGCCAAGGGTGCTTAATAATATCGGGCGGAAc                                              >  2:1342451/1‑151 (MQ=255)
                                                                                                          tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAATGATCAGCCTTAAT‑CAGGGGGCGGGAATTGCCCTCCGGGTGGAGACGCAGAGGTCTCGGTGCGGTTTCAAA‑CGCCAGGCTTGCTGCCTATTggtggggggaac                                              >  2:1590455/1‑139 (MQ=255)
                                                                                                          tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAAAGGACAG‑CCTAATGCGGGGGGGGGTATTTGCCCCCCGCGCGGAGACGAAGGGGGCAGCGTGC‑GGTTTAAAACGCCCGGGATGCTGCATATTTTCGGGGGtga                                               >  2:255509/1‑147 (MQ=255)
                                                                                                           ggCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcgg                                            <  2:81911/151‑1 (MQ=255)
                                                                                                            gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc                                           <  1:778979/151‑1 (MQ=255)
                                                                                                             cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggcg                                          <  2:241451/151‑1 (MQ=255)
                                                                                                               gACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAG‑CTTTATTCGGGGGGGGATATTTCCCCCCCCCGTGCCGACGAAGCGGGCAGCGGGG‑GGTTCAAAAAGCCCGGGATGCTGCATATTGTCcgggggtaccgcggg                                        >  1:1439393/1‑139 (MQ=255)
                                                                                                               gACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAG‑CTTTATGCGGGGGGCGGTATTTGCCCCCCCCGTGCCGACGAAGCGGGCAGCGGGC‑GGTTTCAAGCGCCAGGATTTCTGCCTATTTTCGGGGGGAAcggcggg                                        >  2:22759/1‑150 (MQ=255)
                                                                                                                   tGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTAAAA‑CTTAATACGGGAGGCGATATTTCCCCCCCGCGGGCAGACGAAGCGGTCAGCGGGG‑GGTTCAAAGAGCCCGGGTTGCTGCCTATTTTCGGGGGGAACGGGGGACg                                      >  1:1215532/1‑149 (MQ=255)
                                                                                                                   tGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAG‑CTTAATTCGGGAGGGGATATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGC‑GGTTTAAAACGCCCGGATTGCTGCATATTTTCGGGGGGAAaggcgg                                         >  2:1220023/1‑146 (MQ=255)
                                                                                                                    ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAG‑CTTAATGCGGGGGGGGGTATTTGCCCTCCGCCTGCAGACGAAGCGGGCAGCGGGC‑GTTTCCAAACGCCAGGATTGGTGCATATTTTCGGGCGGAAaggcg                                          >  1:582751/1‑144 (MQ=255)
                                                                                                                      cTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCCCGTGCAGACGAAGCGGGCAGCGGGC‑GTTTCAAAACGCCAGGATTGCTGCATATTGTCGGGCGTAAAGGCGGGCGGaa                                   >  2:1237058/1‑149 (MQ=255)
                                                                                                                       ttCAACCGCTGGAATTACCGGCGGGGGATGGAAGAGTAACG‑CTTTATGCGGGAGGGGAGATTCCCCCCCCCCGGGCAGGAGAAGGGGGCCGCGGGC‑GGGTCAAAGCGCCAGGATTGCTGCATATTGTCGGGGGTGACggcgggcggga                                   >  2:1373521/1‑145 (MQ=255)
                                                                                                                        tCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAG‑CTTAATGCGGGGGGGGGTATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGCGTGC‑GGTTCAAAGGGCCGGGGTTTGTGCATTTTTTCGGGGGGAAcggggggggg                                     >  1:300269/1‑141 (MQ=255)
                                                                                                                        tCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCAAATTGTCGGGCGTAACGGCGGACGTAAgat                                <  2:690541/150‑3 (MQ=255)
                                                                                                                          aaCCGCTGGAATTACCGGCGGGGGTTGGGAGAGTACAG‑CATAAATCGGGGGGGGGTATTTGCCCCCCCCGGGCAGAAGAAGCGGGCAGCCGGC‑GGTTTAAAAAGCCCGGATTTCTGCCTATTTTTGGGGGTAAagggcggcggagaaaaa                              >  1:1284890/1‑136 (MQ=255)
                                                                                                                          aaCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTaaa                             <  2:1289953/151‑1 (MQ=255)
                                                                                                                           aCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAg                            <  1:147507/151‑1 (MQ=255)
                                                                                                                              gCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGaaa                         <  2:663174/151‑1 (MQ=255)
                                                                                                                                 ggAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAAt                       <  2:1267796/150‑1 (MQ=255)
                                                                                                                                         cggcggGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGGAAGCTAAAGAAAATCTgg                   >  2:19821/1‑146 (MQ=255)
                                                                                                                                           gcggGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGc            <  1:945505/151‑1 (MQ=255)
                                                                                                                                            cggGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGc            >  1:420919/1‑150 (MQ=255)
                                                                                                                                            cggGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGc            >  1:1233530/1‑150 (MQ=255)
                                                                                                                                            cggGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCt           <  2:177614/151‑1 (MQ=255)
                                                                                                                                             ggggCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTg          <  1:64024/151‑1 (MQ=255)
                                                                                                                                              gggCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTAAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg         >  2:1335702/1‑151 (MQ=255)
                                                                                                                                                   gggAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGt     <  1:770854/150‑1 (MQ=255)
                                                                                                                                                   gggAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTg    <  1:1161510/151‑1 (MQ=255)
                                                                                                                                                     gAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgcc  >  1:1375733/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
AGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG‑CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCC  >  NC_000913/213191‑213490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑