Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	357,890	0	C	A	42.0%	74.5 / 27.2	50	L267F (TTG→TTT)	cynR	transcriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (23/6);  new base A (0/21);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 5.11e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCA  >  NC_000913/357741‑358034                                                                                                                                                         |                                                                                                                                                    tGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGcaac                                                                                                                                                     >  2:318637/1‑150 (MQ=255)   aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACaaa                                                                                                                                                  <  1:968790/151‑1 (MQ=255)    tAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACaaa                                                                                                                                                  >  1:127642/1‑150 (MQ=255)     aagcgcttcgcATCAGGCAATTCCAGCCGCTGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAc                                                                                                                                                <  1:724464/143‑1 (MQ=255)     aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAc                                                                                                                                                >  2:1690216/1‑151 (MQ=255)       gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAcc                                                                                                                                               <  1:1409167/150‑1 (MQ=255)       gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAc                                                                                                                                                <  2:1250385/149‑1 (MQ=255)        cGTAGCGCATCAGGCCATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAccgc                                                                                                                                             >  2:1053502/1‑151 (MQ=255)                     gCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCagt                                                                                                                                >  1:268393/1‑151 (MQ=255)                        aTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCagtagt                                                                                                                             >  2:1482015/1‑151 (MQ=255)                         ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTg                                                                                                                            >  1:502851/1‑151 (MQ=255)                            cAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                                         >  1:342861/1‑151 (MQ=255)                               cggaagtccTGTGCCGGGGGCTCCCTTGATTCTTTCCCGCAAACACCCGCGCTTTTACCCACCGCCTTGTGAAAAAA‑GCCCTC‑GGGGGCGGCTGTCGGCCAGTTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgg‑gg                                                                                                                     <  2:1005859/144‑1 (MQ=255)                               ccGCAGCCTTGTGCCGGCGCCTCCCGTGTTTCTTTCCCCCCAACACCCGCCCTTTTACCCACCGCAATTTGAAAAAT‑TCCTTT‑CGCGGCGGCTGTCTCCCATTTTTTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                                         <  2:340143/148‑1 (MQ=255)                                  cAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg‑gg                                                                                                                     >  2:234779/1‑148 (MQ=255)                                   aGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg‑ggg                                                                                                                    >  1:828424/1‑147 (MQ=255)                                     cctggtgtCGGCGCCTCCCGTGTTTCATTCCCCCAAACACCCGCGCTTTTCCCCACCCCCATGTCAAAAAA‑TCCCTC‑CGCGGCGGCTTTCTGCCAGCTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCaa                                                                                                                  <  2:58109/144‑1 (MQ=255)                                        tgtgTCAGCGGCTCCGGTGTTTCTTTCCCCCCAACACCCGCGCTTTTACCCAACCCCTTGTAAAAAAA‑TCCCTT‑CGCGGCGGCTGTCTGCAAGCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAgaga                                                                                                              <  2:858750/149‑1 (MQ=255)                                        tgtgTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg‑gggggggg                                                                                                               >  1:48924/1‑142 (MQ=255)                                         gtgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAGGAGaaa                                                                                                            >  2:1353738/1‑150 (MQ=255)                                          tgtCGGCGGCTCCGTTAATTCTTTCCCCCAAACACCCGCGTATTTATCAACCGCAATGTGAAAAAA‑TGCCTG‑GGCGGCGGTTTTCTGCAAGTTTTTTTT‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAagag                                                                                                               <  2:1546326/146‑1 (MQ=255)                                          tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg‑gggcagaaaa                                                                                                             >  2:1329351/1‑140 (MQ=255)                                             cgggggCTCCCGTTTTTCTTTCCCCCAAACACCCGCGCTTTTACCCACCGCCATGTGAAAAAA‑TCCCTT‑CGCGGCGGCTGTCTGCCAGTTATTTTC‑CCCCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAag                                                                                                                 <  2:636280/137‑1 (MQ=255)                                             cAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCACgagagaaa                                                                                                          >  2:1504475/1‑144 (MQ=255)                                                 ggctccggtttttCATTCCCCCCAACACCCGCGTATTTACCCACCGCCATGTAAAAAAA‑TGCCTT‑CGCGGGGGCTGTCTGCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTaa                                                                                                   <  2:467460/140‑1 (MQ=255)                                                   cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGGGAGAGaaaaaaatttt                                                                                                    >  1:1000947/1‑140 (MQ=255)                                                      ccGTTATTCTTTCCCCCCACCACCCGCGCATTTATCCACCGCC‑TGTTAAAAAACTCCCTC‑CGCGGCGGCTGTCTCCCGGTTATTTTC‑CCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGccc                                                                                               <  2:1408215/150‑1 (MQ=255)                                                        gTGATTCATTTCCGCCAAAAACCGCGCATTTATCCAACCCCATGTGCAAAAA‑TCCCTA‑CGCGGCGGCTGTCTGCCAGCTATTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTc                                                                                            <  2:1176847/151‑1 (MQ=255)                                                                ttccccccAACACCCGCCCTTTTACCCACCGCCATGTAAAAAAT‑TGCCTC‑CGGGGCGGTTGTCTGCCAGTTTTTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCAtgt                                                                                        <  2:98028/141‑1 (MQ=255)                                                                   cccccAACACCCGCATTTTTACCCACCGCTATGGGAAAAAT‑TGCCTG‑CGGGGCGGCTGTCTGCCATTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTgg                                                                                 <  2:751113/148‑1 (MQ=255)                                                                       caacaaCCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGGGAGAGAAAAAACttttaaaccccc                                                                                            >  2:994518/1‑127 (MQ=25)                                                                             ccccgtttttACCCACCCCTATGTGAAAAAA‑TCCTTC‑CGCGGCGGCTTTCCGCCAGTTTTTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAAt                                                                             <  2:935348/138‑1 (MQ=255)                                                                             ccGCGATTTTACCCACCGCCATGTGAAAAAA‑TGCCTC‑CGGGGGGGCTGTCTGCCAGTTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctg                                                                        <  2:1001024/150‑1 (MQ=255)                                                                                  cATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGGGAGGGGAAAAAATTTTAACCCCCCCTGTTGTGTggggatagccggtgg                                                                    >  1:42786/1‑136 (MQ=21)                                                                                    tttcccaaccgcattgtgAAAAAA‑TGCCTC‑GGGGGCGGCTTTCTGCCAGCTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGctgc‑tg                                                                    <  1:378935/132‑1 (MQ=255)                                                                                    tttACCCAACCCATTGTAAAAAAG‑TGCTTT‑GGGGGCGCTTGTCGGCCAGTTATTTTC‑CCGCCGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑aa                                                               <  1:220809/150‑1 (MQ=255)                                                                                                cgaggagaaaaa‑aGCCTTCCGGGGCGGCTGTCGGCCAGTTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAg                                                   <  2:895920/144‑1 (MQ=255)                                                                                                      cAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGGGAGAGAAAAAACTTTTAACACCCCCTGTTTTGTGGGAAATGCTGC‑TGGG‑Aaaaa                                                            >  2:1533516/1‑135 (MQ=255)                                                                                                      aaaaaa‑TGCCCC‑CGGGGCGGCTGTCGCCCAGTTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGa                                                <  1:1401790/146‑1 (MQ=255)                                                                                                         aaa‑TGCCTC‑GGGGGCGGCT‑TTTGCCCGGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTgcgg                                         <  2:1532354/151‑1 (MQ=255)                                                                                                           a‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCGACGGAAAAAAACTTAATGCCGCCCTTTTCTGTGGGGAACGCTGC‑GGGTCGAAAATGTGGAAAg                                                   >  2:252001/1‑140 (MQ=255)                                                                                                                 tt‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGGAAGAGAAAAAACCTTAAAACCGCCCTGTTTTGTGGGGATCGCTGC‑TGGT‑GACAAAGTGGAAAGGGGAgggggggggaac                                   >  2:20743/1‑139 (MQ=255)                                                                                                                       ggcggcTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGGAAGAGAAAAAAATTTTAAACCGCCCTGTTTTGTGGGCATTGCTGC‑TGGG‑GAAAAAGTTGGAAGGGGAGGGGGGGGGAACAGCTc                              >  2:1398953/1‑150 (MQ=255)                                                                                                                         cggcTGTCTGCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa                            <  2:214765/150‑1 (MQ=255)                                                                                                                         cggcTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGGGAGAGGAAAAAATTTTAACCCCTCCTGTTGTGTGGGGATCACCGC‑TGGG‑GAAAAAGAGGGAAGGGGGGGGGGGGGGATCAGCTcc                             >  1:634014/1‑149 (MQ=255)                                                                                                                               tctgcagttttttttc‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa                            <  1:120386/133‑1 (MQ=255)                                                                                                                                       cTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGTGGGAGGGAAAAAAGATTTAACCCCGCCAGTTTTTGGGGC‑GTTGCTGCTTGGG‑AAAAATGTGGAAGGGGAGGGGGGGGGAA‑CGACTCCACAACCgcgcacaat              >  1:1214422/1‑145 (MQ=255)                                                                                                                                         ttttcttccgcgcaaacaaATCCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAt               <  2:261810/134‑1 (MQ=255)                                                                                                                                          ttttt‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGGGAGAGAAAAAAATTTTAGACCGCCCCTTTGTGTGGGGATCGCCGC‑TGGG‑GACAATGTGGGAAGGGAAGGGGGGGGGATCCACTCCCGaaaccccccaatt              >  1:601692/1‑136 (MQ=255)                                                                                                                                          ttttc‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGa            <  2:1639110/149‑1 (MQ=255)                                                                                                                                          atttcccgccgataacaaAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGTCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg             <  1:214386/136‑1 (MQ=255)                                                                                                                                                   ccgcAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTc        <  2:783486/145‑1 (MQ=255)                                                                                                                                                   ccgaAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCa  <  2:867853/151‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                    TGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGG‑GGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGC‑TGGT‑AACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCA  >  NC_000913/357741‑358034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑