Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA43.0% 105.1 / 54.1 86intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (46/3);  new base A (0/37);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 3.41e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824906‑1825205
                                                                                                                                                       |                                                                                                                                                     
aCCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTgagaagag                                                                                                                                                       <  2:101764/151‑1 (MQ=255)
 ccGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTgagaagaga                                                                                                                                                      <  2:1678732/151‑1 (MQ=255)
  cGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTgagaagaga                                                                                                                                                      >  1:221605/1‑150 (MQ=255)
    aCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGagaaa                                                                                                                                                    >  1:1347148/1‑150 (MQ=255)
       ttttACGCATATCAAAGTATTCCATGCTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGagaaaaga                                                                                                                                                 >  2:505907/1‑150 (MQ=255)
        tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGagaaaagaa                                                                                                                                                <  1:889582/150‑1 (MQ=255)
        tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGagaaaagaa                                                                                                                                                >  2:1667922/1‑150 (MQ=255)
         ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGagaaaagaaa                                                                                                                                               <  2:982873/150‑1 (MQ=255)
          tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAAc                                                                                                                                             >  2:305315/1‑151 (MQ=255)
            cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                           >  2:370968/1‑151 (MQ=255)
              cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAAccgcc                                                                                                                                         >  2:865492/1‑151 (MQ=255)
                   cAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATcc                                                                                                                                    >  1:1633334/1‑151 (MQ=255)
                     aaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                                  >  1:1374394/1‑151 (MQ=255)
                     aaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                                  >  2:870096/1‑151 (MQ=255)
                        tATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                               >  1:672233/1‑151 (MQ=255)
                        tATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                               >  1:518807/1‑151 (MQ=255)
                        tATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                               >  1:1046504/1‑151 (MQ=255)
                         aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGTTCCTGTcc                                                                                                                               >  2:1022726/1‑150 (MQ=255)
                         aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                               >  2:1695570/1‑150 (MQ=255)
                          ttCCATCCTCGCCCCCGGCCTTTTTTTTTCTTTTTTTTCGTTCAGGTACCGGTGGTTGTTTATCGGCAACTGGGGTGTTTTAGGTTAGGGGTGATCGGTTTTTTTTTACA‑ATTTTTGGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCa                                                                                                                              <  1:491940/150‑1 (MQ=255)
                             cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                          >  1:1493899/1‑151 (MQ=255)
                              aTACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                          >  1:1594654/1‑150 (MQ=255)
                               tACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                        >  2:148526/1‑151 (MQ=255)
                               tACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                        >  2:586628/1‑151 (MQ=255)
                                ccTCGCTCCCGGCCTGTTTTTTTCTTTTTTATCTTTCGGGTACCCGGGGATGTTTTCCGGCAATTGGGGTATTTTACGTTAGTGGTGATCGGGTTTTTTTTACA‑CTTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                        <  1:1364860/149‑1 (MQ=255)
                                   cGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                    >  1:1625640/1‑151 (MQ=255)
                                   cGCCCCCGGCCCTTTTTTTTCATTTTTATCGTTCAGGTACCCGTGGATGTTTACCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACA‑ACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                     <  2:410039/150‑1 (MQ=255)
                                    gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                   >  1:1379835/1‑151 (MQ=255)
                                    gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                   >  1:699667/1‑151 (MQ=255)
                                    gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                   >  2:330246/1‑151 (MQ=255)
                                     cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                  >  2:1502775/1‑151 (MQ=255)
                                     cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                  >  2:827329/1‑151 (MQ=255)
                                      tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                  >  2:187884/1‑150 (MQ=255)
                                        ccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                                 >  1:590393/1‑149 (MQ=255)
                                         cGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                              >  1:1361091/1‑151 (MQ=255)
                                            cccTTTTTTTTCTTTTTTTTCGTTAGGGTAACGGGGGATGTTTACCGGCAACTGGGGTGATTTACGTTGGTGTTGATCAGTTTTTTTTTACA‑ACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                             <  2:1156417/149‑1 (MQ=255)
                                            cccTTTTTTTTCTTTTTTATCTTTAAGGTACCGGTGGTTGTTTCTCGGCACCGGGGGTAATTTACGTTGGGGGTGATCGGTTTTTTTTAACA‑ACTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                              <  2:396236/148‑1 (MQ=255)
                                                tttttttCATTTTTATCTTTAAGGTAACCGTGGATGTTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGTTTTTTTTTACA‑CCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                         <  2:71790/146‑1 (MQ=255)
                                                ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTCCAAGGTAGTgg                                                                                                        >  2:858302/1‑150 (MQ=255)
                                                 ttttttCTTTTTTATCGTTCGGGAACCGGTGGTTGTTTATCGGAAACGGGGGTGATTTACTTTAGTGGTGATCGGGTTTTTTTTACA‑ATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGa                                                                                                       <  1:546408/148‑1 (MQ=255)
                                                  cTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                      >  2:1026866/1‑150 (MQ=255)
                                                   ttttCTTTTTTATTGTTCAGGTAACGGTGGAGGGTTATCGGCACCGGGGGTGTTTTAGGTTAGTGGTGACCAGGTTTTTTTTCCA‑ACTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                     <  2:487554/150‑1 (MQ=255)
                                                    tttaatttttttgtttCAGGAACCGGGGGATGGTTGTCGGCACCTGGGGTTTTTTAGTTTAGTGGTGATCAGGTTTTTTTAACA‑CCTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACaa                                                                                                    <  1:159159/136‑1 (MQ=255)
                                                    tttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                     >  2:473529/1‑149 (MQ=255)
                                                         ttGTTTTCGTTCAGTTAACCGTGGATGGTTAGGGAAAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACA‑ACTGTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                <  2:1284011/149‑1 (MQ=255)
                                                            ttttCGTTCAGGTACCCGTGGTTGTTTATCGGCAACTGGGTTGATTTACTTTAGGGGTGATCAGGTTTTTTTTACA‑ACTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                             <  1:111775/146‑1 (MQ=255)
                                                            ttATCTTTCAGGTAACGGTGGATGGTTACCGGCAACTGGGGTGTTTTACGTTAGTGTTGATCAGTTTTTTTTTACA‑GTTTTTGGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                             <  2:1568170/149‑1 (MQ=255)
                                                             tATCGTACAGTTAACGGGGGATGTTTATCGGCACCGCGGGTGTTTTACGTTGGTGGTGATCGGGTTTTTTTAACA‑CTTTTTGGAGAAGAGAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                           <  2:162602/150‑1 (MQ=255)
                                                              aTCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                          >  1:469507/1‑150 (MQ=255)
                                                                agTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                       >  1:1123523/2‑151 (MQ=255)
                                                                 gTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                       >  1:118124/1‑150 (MQ=255)
                                                                  ttaaggacccggTGGTTGGTTAGCGCCACCGGGGGTATTTTACTTTGGTGGAGAGCGGTTTTTTTTTCCA‑TTTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                       <  1:378632/138‑1 (MQ=255)
                                                                  ttCAGGTACCGGTGGATGTTTATCGGCAACTGGGGTGTTTTACGTTGGTGGTGATCGGTTTTTTTTTACA‑ACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                          <  2:1332749/146‑1 (MQ=255)
                                                                    aggttgggtgTGGTTGGTTATCGAGAAC‑CGGGGTTTTTATGTTGGGGTTGGGCGGGTTTTTTTTACATACTGTTGGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                       <  1:927605/138‑1 (MQ=255)
                                                                         accggTGGATGTTTATCGGCACCTGGGGTGTTTTACGTTAGGGGTGACCGGGTTTTTTTAACA‑ATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGt                                                                                   <  2:714648/142‑1 (MQ=255)
                                                                         aaCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                              >  2:545125/1‑151 (MQ=255)
                                                                            cGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                            >  2:1267249/1‑150 (MQ=255)
                                                                                  tGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGGGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTgga                                                                       >  1:401045/1‑147 (MQ=255)
                                                                                     ttATCGGCACCTGGGTTGATTTACTTTGGTGGTGATCAGTTTTTTTTTAAA‑ACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                                   <  1:1710318/150‑1 (MQ=255)
                                                                                       aTCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattat                                                                >  2:1163088/1‑151 (MQ=255)
                                                                                          ggCAACTGGGGTGATTTACGTTAGTGGTGATCGGTTTTTTTTTACA‑TTTTTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                               <  1:148517/149‑1 (MQ=255)
                                                                                          ggCAACTGGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑CTTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                             <  2:1184496/151‑1 (MQ=255)
                                                                                             acctggggTAATTTACGTAGGGGGTGAGCGGTTTTTTTTTACA‑ATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                              <  1:227448/142‑1 (MQ=255)
                                                                                             aaCTGGGGTTATTTCCTTTAGGGGTAATCAGGTTTTTTTTACA‑ACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                             <  2:1489240/148‑1 (MQ=255)
                                                                                             aaCTCGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTAAA‑ACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                              <  1:711745/147‑1 (MQ=255)
                                                                                                  gggTGATTTACGTTGGTGTTGATCAGGTTTTTTTTACA‑ACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                       <  2:1212943/149‑1 (MQ=255)
                                                                                                  gggTGATTTACGTTAGTGGTGATCAGGTTTTTTTAACA‑ATTGTTAAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                     <  1:1682498/151‑1 (MQ=255)
                                                                                                    gTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                       >  2:78420/1‑147 (MQ=255)
                                                                                                       aTTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAgg                                                  >  1:1679831/1‑149 (MQ=255)
                                                                                                        tttAGGTTAGTGGTAACCTGTTTTTTTTAACA‑ACTGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAgg                                                  <  1:1711664/148‑1 (MQ=255)
                                                                                                          tACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCAttt                                             >  1:907567/1‑151 (MQ=255)
                                                                                                              tgattggtgACAAGGTTTTTTTTACA‑ACTGTTAGAGAAAAAAAAAGAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtt                                              <  2:62517/142‑1 (MQ=255)
                                                                                                                aGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                       >  1:1538738/1‑151 (MQ=255)
                                                                                                                  tggtgATCGGTTTTTTTTTACA‑TCTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                      <  1:505855/150‑1 (MQ=255)
                                                                                                                   gttgatgaGGTTTTTTTAACA‑ACTGTTTAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt                                        <  2:1347241/145‑1 (MQ=255)
                                                                                                                   ggtaATCAGTTTTTTTTTACA‑ATTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg                                    <  2:86498/151‑1 (MQ=255)
                                                                                                                         cAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCt                               >  2:23303/1‑150 (MQ=255)
                                                                                                                          ggtttttttttACA‑TCTTTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                             <  1:1651548/147‑1 (MQ=255)
                                                                                                                          gggttttttttACA‑CCTTTTGGAAAAAGAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                             <  1:570319/147‑1 (MQ=255)
                                                                                                                          aGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                             >  2:706632/1‑151 (MQ=255)
                                                                                                                           gtttttttttACA‑ACTGTTGAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGc                                <  1:870029/144‑1 (MQ=255)
                                                                                                                              tttttttAAA‑TTTGTGTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAct                          <  1:865425/150‑2 (MQ=255)
                                                                                                                                tttttACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                        >  1:419805/1‑150 (MQ=255)
                                                                                                                                  tttACA‑ATTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGCTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                      <  1:1026789/150‑1 (MQ=255)
                                                                                                                                   ttACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                    >  2:924845/1‑151 (MQ=255)
                                                                                                                                    tACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                    >  1:261041/1‑150 (MQ=255)
                                                                                                                                    tACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCGGCTGGACGTTCTGTc                   >  2:879352/1‑151 (MQ=255)
                                                                                                                                     aCA‑ACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                   <  2:1594750/150‑1 (MQ=255)
                                                                                                                                         aCTTTTTGAGAAAAGAAAAAAACGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc               <  2:401074/151‑1 (MQ=255)
                                                                                                                                           tGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg              <  2:1046583/150‑1 (MQ=255)
                                                                                                                                           tGTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg              <  2:1361183/150‑1 (MQ=255)
                                                                                                                                             tttGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg              <  2:134165/148‑1 (MQ=255)
                                                                                                                                             ttgaagaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt            <  2:1500603/146‑1 (MQ=255)
                                                                                                                                                 agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc        >  2:1076499/1‑150 (MQ=255)
                                                                                                                                                 agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc        >  2:1425199/1‑150 (MQ=255)
                                                                                                                                                   aagaaaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt           <  1:1691574/145‑1 (MQ=255)
                                                                                                                                                   aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa     <  1:370940/151‑1 (MQ=255)
                                                                                                                                                   aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa     <  1:1695455/151‑1 (MQ=255)
                                                                                                                                                      agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt  >  1:206190/1‑151 (MQ=255)
                                                                                                                                                       gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt  >  2:1085775/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
ACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACA‑ACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824906‑1825205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑