Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,434,8820TG42.3% 192.6 / 35.5 105Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (9/51);  new base G (44/0);  total (54/51)
Fisher's exact test for biased strand distribution p-value = 9.77e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434733‑2435032
                                                                                                                                                     |                                                                                                                                                      
cAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTa                                                                                                                                                       <  1:1276637/151‑1 (MQ=255)
      cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGGCAGGGGAATACa                                                                                                                                                  >  2:224773/1‑150 (MQ=255)
         atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGaaatcccccc                                                                                                                                              >  2:1461080/1‑143 (MQ=255)
         atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcg                                                                                                                                               <  1:150011/150‑1 (MQ=255)
            tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGa                                                                                                                                            <  1:1212235/150‑1 (MQ=255)
            tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGa                                                                                                                                            <  2:663480/150‑1 (MQ=255)
             gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAc                                                                                                                                           <  2:530885/150‑1 (MQ=255)
               cTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGTCAGGGTAATCCACCGCGAcc                                                                                                                                          >  2:1072779/1‑149 (MQ=255)
               cTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGGcc                                                                                                                                          >  2:1345061/1‑149 (MQ=255)
                 aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCCGGCAGGGGAATCCACCGCGGcc                                                                                                                                          >  1:235929/1‑147 (MQ=255)
                 aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAt                                                                                                                                      <  2:760957/151‑1 (MQ=255)
                 aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAt                                                                                                                                      <  2:874435/151‑1 (MQ=255)
                   gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATa                                                                                                                                     <  1:895845/150‑1 (MQ=255)
                       tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGc                                                                                                                                 <  2:349826/150‑1 (MQ=255)
                          aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCtt                                                                                                                             <  1:1460981/151‑1 (MQ=255)
                          aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCtt                                                                                                                             <  2:337012/151‑1 (MQ=255)
                            tCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGACCCGATACAGCCCTTcc                                                                                                                           >  2:230245/1‑151 (MQ=255)
                             cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTcc                                                                                                                           >  2:431036/1‑150 (MQ=255)
                             cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATTCACCGCGACCAGATACAGCCCCTcc                                                                                                                           >  2:274981/1‑150 (MQ=255)
                               tCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCCCGACCAAATACAGCCCTTcc                                                                                                                           >  2:684525/1‑148 (MQ=255)
                                cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCtt                                                                                                                       <  1:1223267/151‑1 (MQ=255)
                                 aTCTTTCAGGCTGTGACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGGCAGGGGAATCCACCCCGACCAGAAACAACCCCTCCGCctt                                                                                                                      >  1:1464973/1‑151 (MQ=255)
                                        aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGCTTTTTCCGtt                                                                                                               >  2:313986/1‑151 (MQ=255)
                                        aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAATACAAACCCGGCCGGGGGATTCACCGCGAACCAACACAGCCCCTCCCCttttcgcggt                                                                                                               >  2:1386271/1‑145 (MQ=255)
                                             gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgct                                                                                                            <  2:869878/149‑1 (MQ=255)
                                              tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGAAACAGCCCTTCCGCTTTTTCCGGTGTTgcc                                                                                                         >  1:1708647/1‑151 (MQ=255)
                                                aCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACGGTCAAGGGAATCCACCGCGACCAGATAAAACCCCTCCGCCTTTTTCGTTGCTgcc                                                                                                         >  2:1492/1‑149 (MQ=255)
                                                   aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCGGGCAGGGGAATCCACCGCGGCCAGAAACAGCCCCTCCGCCTTTTCCGGTGCTgccgcc                                                                                                      >  2:1147658/1‑149 (MQ=255)
                                                    cGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAACATACCCGTCAGGGGAATACACCGCCACCAAATACAGCCCCTCCCCTTTTTCCGTTTCGgcccccc                                                                                                     >  2:1307063/1‑148 (MQ=255)
                                                       tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGACCAGATACAACCCCTCCCCCTTTTTCGTTGCTGccgcccgc                                                                                                   >  2:1035886/1‑148 (MQ=255)
                                                       tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGc                                                                                                   <  1:1035809/148‑1 (MQ=255)
                                                           tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCGCTTTTGCCCTTGCTGCCGCCCGCGGTCt                                                                                              >  2:1582576/1‑149 (MQ=255)
                                                              aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTATACCGGGCAGGGGAATCCACCCCGACCCGATACAGCCCCTCCCCCTTTTCCCGTGCTGCCCCCCCCGGTCTGTcc                                                                                          >  1:734352/1‑150 (MQ=255)
                                                               gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCtt                                                                                        <  2:746410/151‑1 (MQ=255)
                                                                ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGATCATACCGGTCAGGGGAAACCACCCCGCCCACAAACAGCCCCTCCCCCTTTTCCGTTGCTGCCGCCCGCGGTCCTTCCttt                                                                                       >  1:471689/1‑151 (MQ=255)
                                                                ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCCCTTTTTCCGTTGCTTCCCCCCGCGTTCTGTCCttt                                                                                       >  2:1310961/1‑151 (MQ=255)
                                                                  tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATAATAACGGGCGGGGGAATCCACCCCGACCAGAAACAGCCCCTCCGCTTTTTTCCTTGCTTGCCCCCGCGGTCTTTCCttt                                                                                       >  1:1615317/1‑149 (MQ=255)
                                                                  tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGACCAGATACAGCCCCTCCCCCTTTTCCGTTGCTGCCCCCCGCGTTCTGTCCttt                                                                                       >  1:672462/1‑149 (MQ=255)
                                                                   gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGGCACGGTAATCCACCGCGACCAGATAAAGCCCCTCCCCTTTTTCCGCTGCTGCCGCCCGCGGTCTGTCCCTTtcc                                                                                    >  2:155188/1‑151 (MQ=255)
                                                                     aaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTgccgc                                                                                  <  2:1604256/151‑1 (MQ=255)
                                                                       tGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTGAGGGGAATCCACCCCGACCCGATAAAACCCCTCCGCTTTTTCCGGTGCTGACGCCAGCGTTCTGTCCTTTtccgcc                                                                                 >  1:1577499/1‑150 (MQ=255)
                                                                         ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTGGATGAAAACGGGGGGGGGAAACCACCGCGGACAGATAGCGCCCTTTCCCTTTTGCCCGTGCTGCCGCCCGCGTTCTCTTCttttgccccag                                                                               >  2:324490/1‑142 (MQ=255)
                                                                          tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACCGGCAGGGGAATCCACCCCGCCCCGATACAACCCTTCCCCCTTTTCCGTTGCTGCCGCCAGCGGTCTGTTCTTTtccgcc                                                                                 >  2:1252977/1‑147 (MQ=255)
                                                                          tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGc                                                                              <  2:1320675/150‑1 (MQ=255)
                                                                          tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGCTTTGGGAGAATATAACGGGCAGGGGGATACACCGCGCACAGATAAAGGCCTTCCGGCTTTGCCGGTGCTGCAGCCAGCGGTCTGTCCtt                                                                                        >  2:276590/1‑140 (MQ=255)
                                                                           ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCa                                                                             <  1:566690/150‑1 (MQ=255)
                                                                            cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGTCCGGGGAATCCCCCGCGACCAGATACAGACCCTCCGCTTTTGCCGTTGCTGCCCCCCGCGGTCTGTCCTTTTCCCCCAGCaa                                                                            >  2:1695269/1‑150 (MQ=255)
                                                                            cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCCCTTTTTCCGTTGCTGCCGCCCGCGTTTTGTCCTTTtccgccc                                                                                >  2:463205/1‑145 (MQ=255)
                                                                               gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCCGGGGAATCCACCGCGACCCGATACAGCCCCTCCGCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCCTTTTCCGCCAGCAActc                                                                         >  2:960191/1‑150 (MQ=255)
                                                                                 attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAAACCACCGCGGCCAGATACAACCCTTCCCCTTTTTCCGTTGCTGCCCCCAGCGTTCTGTCCTTTTCCGCCCGCaa                                                                            >  2:750597/1‑145 (MQ=255)
                                                                                 attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCCCTTTTTCCGTTGCTGCCGCCAGCGGTCTGTCCTTTTCCGCCCGCAAc                                                                           >  2:867874/1‑146 (MQ=255)
                                                                                  ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTTCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCCGCAActct                                                                        >  1:591875/1‑148 (MQ=255)
                                                                                    attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTa                                                                    <  1:648304/150‑1 (MQ=255)
                                                                                    attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTa                                                                    <  1:827100/150‑1 (MQ=255)
                                                                                     ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAAGGGAATCCACCGCGACCAGAAACAACCCCTCCCCTTTTTCCGTTTCTGCCCCCCCCGCTCTGTTCTTTTCCCCCAGCAACTCTCCTAt                                                                   >  2:334342/1‑150 (MQ=255)
                                                                                      tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATc                                                                  <  2:86525/150‑1 (MQ=255)
                                                                                      tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCCCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCCTTTTCCGCCCGCAACTCTGCTATcc                                                                 >  2:38902/1‑151 (MQ=255)
                                                                                       ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAAACCACCGCGACCCGATACAGCCCTTCCGCTTTTGCCGTTTCTGCCGCCCGCGGTCTGTCCTTTTCCGCCCGCAACTCTGCTATcc                                                                 >  1:1100926/1‑150 (MQ=255)
                                                                                        tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCGCTTTTGCCGTTGCTGCCGCCAGCGGTCTGTTCTTTGCCGCCAGCCACTCTTCTATcc                                                                 >  2:1221375/1‑149 (MQ=255)
                                                                                        tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGGAGATCATACCCGTCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCCCTTTTTTCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCCCCCGCAACTCTGCTATcc                                                                 >  2:1458937/1‑149 (MQ=255)
                                                                                           ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATAAAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCCGCAACTCTGCTATCCAGctc                                                            >  2:242170/1‑151 (MQ=255)
                                                                                            tAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACAAACCGGGCAGGGGTATACACCGCGACCAGATAAAGCCCTTCCGCTTTTGCCGTTGGCTCCCCCCGCGTTCTGTCCTTTTCCGCCACCAACCCTGccctcc                                                                 >  2:1600514/1‑140 (MQ=255)
                                                                                              gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCCCTTTTTCCGTTTCTGCCCCCCGCGTTCTGTCCTTTTCCGCCAGCAAATCTGCTATTCAGCTCTcc                                                         >  2:1331411/1‑151 (MQ=255)
                                                                                              gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGTAATCCCCCCCGGCCAGATACAGCCCCTCCCCTTTTTCCCTTGCTGCCCCCCGCGGTCTGTCCTTTTCCCCCCGCAACTCTTCTATCCAGCTCTcc                                                         >  2:610625/1‑151 (MQ=255)
                                                                                                ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCCGGGGAATCCACCCCGACCAGATAAAACCCCTTCGCCTTTTCCGGTGCTGCCCCCAGCGGTCTGTCCTTTTCCGCCCGCAACTCTGCTATCCCGCTCTcc                                                         >  1:922946/1‑149 (MQ=255)
                                                                                                    cAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTgg                                                   <  2:251382/151‑1 (MQ=255)
                                                                                                       aaaTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtg                                                <  1:1466366/151‑1 (MQ=255)
                                                                                                        aaTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgt                                               <  2:1476670/151‑1 (MQ=255)
                                                                                                         aTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgtg                                              <  1:938822/151‑1 (MQ=255)
                                                                                                           aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTggg                                            <  2:1650168/151‑1 (MQ=255)
                                                                                                                 cccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGa                                      <  1:1310867/151‑1 (MQ=255)
                                                                                                                 cccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGa                                      <  1:437850/151‑1 (MQ=255)
                                                                                                                       ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCa                                <  2:102430/151‑1 (MQ=255)
                                                                                                                       ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCa                                <  1:132186/151‑1 (MQ=255)
                                                                                                                        gCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCa                                <  1:802374/150‑1 (MQ=255)
                                                                                                                           gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                              >  1:1121982/1‑149 (MQ=255)
                                                                                                                           gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                              >  1:711254/1‑149 (MQ=255)
                                                                                                                           gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAg                            <  2:1536823/151‑1 (MQ=255)
                                                                                                                               tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                        >  1:1140422/1‑151 (MQ=255)
                                                                                                                                ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                        <  2:1083745/150‑1 (MQ=255)
                                                                                                                                ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                        <  1:1074247/150‑1 (MQ=255)
                                                                                                                                ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGc                       <  1:334316/151‑1 (MQ=255)
                                                                                                                                  aaGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                     <  2:609312/151‑1 (MQ=255)
                                                                                                                                  aaGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                     <  2:1305895/151‑1 (MQ=255)
                                                                                                                                       cATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACaa                 <  2:1695266/150‑1 (MQ=255)
                                                                                                                                       cATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat                <  2:178997/151‑1 (MQ=255)
                                                                                                                                          aCCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAatat              <  1:1000873/150‑1 (MQ=255)
                                                                                                                                          aCCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt             <  1:89545/151‑1 (MQ=255)
                                                                                                                                           ccGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt             <  1:1641605/150‑1 (MQ=255)
                                                                                                                                            cGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc           >  1:1634612/1‑151 (MQ=255)
                                                                                                                                            cGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc           <  1:867807/151‑1 (MQ=255)
                                                                                                                                            cGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGAACCGACTTCCATCAGGCTGCCGACAATATTcc           <  2:413070/151‑1 (MQ=255)
                                                                                                                                              gTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg         >  2:1529325/1‑151 (MQ=255)
                                                                                                                                               tCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg         >  1:808259/1‑150 (MQ=255)
                                                                                                                                                cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGa        <  1:1344968/150‑1 (MQ=255)
                                                                                                                                                cAGGGCAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc       >  1:425488/1‑151 (MQ=255)
                                                                                                                                                 aGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc      <  2:652680/151‑1 (MQ=255)
                                                                                                                                                    gTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTTCTGACCata   <  1:230224/151‑1 (MQ=255)
                                                                                                                                                    gTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCata   <  2:1216252/151‑1 (MQ=255)
                                                                                                                                                     tAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCatat  >  1:1397184/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434733‑2435032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: