Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 530,975 C→T R84R (CGG→CGA mnmH ← tRNA 2‑selenouridine synthase, selenophosphate‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913530,9750CT98.0% 372.7 / ‑1.8 99R84R (CGG→CGAmnmHtRNA 2‑selenouridine synthase, selenophosphate‑dependent
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (54/43);  total (56/43)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

CGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAGC  >  NC_000913/530831‑531118
                                                                                                                                                |                                                                                                                                               
cgcgGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCa                                                                                                                                            <  2:67695/150‑1 (MQ=255)
   ggTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCg                                                                                                                                        <  2:884071/151‑1 (MQ=255)
    gTCTGCCGCAATGCCTTAAAACCGCCTTCCACCGGCGAATAATCAATCCCCGCTGCATGCACCCAGCTTTGCACAATATTTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCg                                                                                                                                        <  2:331646/150‑1 (MQ=255)
     tCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTc                                                                                                                                      >  2:1548865/1‑151 (MQ=255)
       tGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTcc                                                                                                                                     >  2:1571423/1‑150 (MQ=255)
        ggCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTcc                                                                                                                                     <  1:525133/149‑1 (MQ=255)
        ggCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCAt                                                                                                                                   >  2:1407660/1‑151 (MQ=255)
          cgcAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCAt                                                                                                                                   >  2:1406912/1‑149 (MQ=255)
                 ccTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGctgctg                                                                                                                          <  1:1123251/151‑1 (MQ=255)
                   ttATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGAc                                                                                                                        >  1:1079841/1‑151 (MQ=255)
                    tataACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACg                                                                                                                       >  2:394328/1‑151 (MQ=255)
                       aaCCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAAt                                                                                                                    >  1:961104/1‑151 (MQ=255)
                       aaCCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAAt                                                                                                                    <  2:1115156/151‑1 (MQ=255)
                         ccGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAAttt                                                                                                                  >  2:777212/1‑151 (MQ=255)
                         ccGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAAttt                                                                                                                  <  2:682966/151‑1 (MQ=255)
                          cGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTc                                                                                                                 >  2:232765/1‑151 (MQ=255)
                          cGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTc                                                                                                                 >  2:906647/1‑151 (MQ=255)
                            ccTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAAAATGTGGGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCAc                                                                                                               <  1:1693316/151‑1 (MQ=255)
                                   ccaGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGccac                                                                                                        >  1:1306274/1‑151 (MQ=255)
                                     aGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGccacc                                                                                                       >  2:11888/1‑150 (MQ=255)
                                           tAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTAt                                                                                                >  1:1262350/1‑151 (MQ=255)
                                            aatcaatcCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATg                                                                                               >  2:1576172/1‑151 (MQ=255)
                                                  tcCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTccc                                                                                           >  2:684287/1‑149 (MQ=255)
                                                   ccccGCTGCATGCACCCAGCTTTGCACAATATGTGGGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCcca                                                                                          <  2:961178/149‑1 (MQ=255)
                                                         tgcatgcaACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCccagcgccagc                                                                                  >  1:1224861/1‑151 (MQ=255)
                                                         tgcatgcaACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCccagcgccagc                                                                                  >  2:895050/1‑151 (MQ=255)
                                                           catgcaACCAGTTTTGCACAATAGGTGAGCGCTGCCCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCcagcgccagcgc                                                                                <  1:919441/151‑1 (MQ=255)
                                                            atgcaACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCt                                                                               >  2:1537068/1‑151 (MQ=255)
                                                              gcaACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGc                                                                             >  2:15648/1‑151 (MQ=255)
                                                                acccATCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTTCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCg                                                                            <  2:1298124/148‑1 (MQ=255)
                                                                aaCCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGc                                                                             <  2:1704611/149‑1 (MQ=255)
                                                                aaCCAGCTTTGCAAAATATGTGAGCGCTGCCCGCCACGGGCGCAGCAGAGAATGCCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTg                                                                              <  1:989911/148‑1 (MQ=255)
                                                                 aCCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTc                                                                          >  1:193412/1‑151 (MQ=255)
                                                                  ccAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCt                                                                         >  2:1514916/1‑151 (MQ=255)
                                                                     gCTTTGCAAAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGa                                                                       <  2:1573842/150‑1 (MQ=255)
                                                                      cTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGc                                                                     >  1:934731/1‑151 (MQ=255)
                                                                       tttGCACAATATGTGAGCGTTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAg                                                                      <  1:957564/149‑1 (MQ=255)
                                                                         tGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCtt                                                                  >  2:1308353/1‑151 (MQ=255)
                                                                          gCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCt                                                                   <  2:1063476/149‑1 (MQ=255)
                                                                          gCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTg                                                                 >  2:660377/1‑151 (MQ=255)
                                                                           cacaATATTGGAGCGCTGCCCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCGGGCTTCCATGCGCTGCTGACGAATTTCGCCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCt                                                                   <  2:525908/148‑1 (MQ=255)
                                                                           cacaATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGc                                                                <  2:1230901/151‑1 (MQ=255)
                                                                            acaATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCt                                                               <  1:138764/151‑1 (MQ=255)
                                                                             caATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCt                                                               <  2:96405/150‑1 (MQ=255)
                                                                             caATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTg                                                              >  2:1714620/1‑151 (MQ=255)
                                                                             caATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTg                                                              >  2:1286175/1‑151 (MQ=255)
                                                                              aaTATGTGGGCGCGGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTg                                                              <  1:1468040/150‑1 (MQ=255)
                                                                              aaTATGTGAGCGCTGCCCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGt                                                             <  1:424555/151‑1 (MQ=255)
                                                                              aaTATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGt                                                             >  1:1698203/1‑151 (MQ=255)
                                                                               atatGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATTCCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGtt                                                            >  2:1415920/1‑151 (MQ=255)
                                                                                tatGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGttt                                                           >  1:1405760/1‑151 (MQ=255)
                                                                                  tgtgAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTtat                                                         >  2:1205644/1‑151 (MQ=255)
                                                                                        cgcTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAgg                                                   >  2:63119/1‑151 (MQ=255)
                                                                                         gcTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAgg                                                   >  2:1087317/1‑150 (MQ=255)
                                                                                           tGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGc                                                >  2:277507/1‑151 (MQ=255)
                                                                                           tGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGc                                                >  1:65652/1‑151 (MQ=255)
                                                                                              ccGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCaa                                             <  2:650664/151‑1 (MQ=255)
                                                                                              ccGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCa                                              <  2:492546/150‑1 (MQ=255)
                                                                                              ccGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCa                                              <  2:681656/150‑1 (MQ=255)
                                                                                               cGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAAc                                            <  1:209600/151‑1 (MQ=255)
                                                                                                  cACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAAcggc                                         >  2:954087/1‑151 (MQ=255)
                                                                                                     gggCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACggcggc                                      >  1:1485049/1‑151 (MQ=255)
                                                                                                     gggCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACggcggc                                      <  2:1105559/151‑1 (MQ=255)
                                                                                                     gggCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACggcggc                                      >  2:1231230/1‑151 (MQ=255)
                                                                                                      ggCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGgcggcg                                     <  1:1379527/151‑1 (MQ=255)
                                                                                                        cgcAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGgcgc                                    <  2:531993/150‑1 (MQ=255)
                                                                                                        cgcAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGcgcg                                   >  2:291010/1‑151 (MQ=255)
                                                                                                         gcagcaGAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGt                                  >  2:1520563/1‑151 (MQ=255)
                                                                                                          cagcagAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGtt                                 >  2:1515434/1‑151 (MQ=255)
                                                                                                            gcagAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTc                                >  1:1653240/1‑150 (MQ=255)
                                                                                                            gcagAGAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCa                               <  1:1534183/151‑1 (MQ=255)
                                                                                                              agagAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATc                             >  2:141083/1‑151 (MQ=255)
                                                                                                              agagAATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATc                             >  1:865000/1‑151 (MQ=255)
                                                                                                                agaATACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGATTATAGCAGGTGCCAACGGCGGCGCGTTCATCGt                           >  1:741672/1‑151 (MQ=255)
                                                                                                                    tACCTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTAtt                       >  1:831799/1‑151 (MQ=255)
                                                                                                                      ccTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTc                      >  1:1391890/1‑150 (MQ=255)
                                                                                                                      ccTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCa                     <  1:1548769/151‑1 (MQ=255)
                                                                                                                      ccTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCa                     <  2:1031131/151‑1 (MQ=255)
                                                                                                                       cTTGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCAt                    >  1:1258468/1‑151 (MQ=255)
                                                                                                                        ttGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCAtt                   >  1:106584/1‑151 (MQ=255)
                                                                                                                         tGCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCAtt                   >  1:1604114/1‑150 (MQ=255)
                                                                                                                          gCGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTa                  >  1:909300/1‑150 (MQ=255)
                                                                                                                           cGGATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTaa                 <  1:232744/150‑1 (MQ=255)
                                                                                                                            ggATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTa                  <  1:764345/148‑1 (MQ=255)
                                                                                                                            ggATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACg               >  2:1448155/1‑151 (MQ=255)
                                                                                                                             gATTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACgg              >  2:931786/1‑151 (MQ=255)
                                                                                                                              aTTTTGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGc             >  2:129239/1‑151 (MQ=255)
                                                                                                                                tttGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCa            <  1:335388/150‑1 (MQ=255)
                                                                                                                                  tGCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGc             >  2:1503404/1‑147 (MQ=255)
                                                                                                                                   gCAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGAtt        <  2:1079928/151‑1 (MQ=255)
                                                                                                                                    cAGGCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTg       <  1:753930/151‑1 (MQ=255)
                                                                                                                                      ggCACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGa      <  1:1311837/150‑1 (MQ=255)
                                                                                                                                      ggCACGCTGCTCCCCAGGCGCCCATGCGCTGCTGACGAATTTCCCCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGATCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGa      <  1:166093/150‑1 (MQ=255)
                                                                                                                                        cACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCCCCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAg   <  1:677295/151‑1 (MQ=255)
                                                                                                                                        cACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGAt     >  1:1696208/1‑149 (MQ=255)
                                                                                                                                        cACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATa    <  1:394300/150‑1 (MQ=255)
                                                                                                                                        cACGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAg   <  1:1682454/151‑1 (MQ=255)
                                                                                                                                         aCGCTGCCCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAgc  >  1:148830/1‑151 (MQ=255)
                                                                                                                                          cGCTGCTCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAgc  <  1:603174/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCGCTGCTGACGAATTTCACCCGCCACCAGTTTATGTCCCAGCGCCAGCGCTGCGTCTGAGCCTTGCTGTTTATAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAGC  >  NC_000913/530831‑531118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: