Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,385,581 T→C V15A (GTT→GCT)  yhcN → cadmium and peroxide resistance protein, stress‑induced

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,385,5810TC99.2% 487.1 / ‑4.4 131V15A (GTT→GCT) yhcNcadmium and peroxide resistance protein, stress‑induced
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (53/77);  total (53/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-01

Only 100 of 134 total aligned reads displayed.

CAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGG  >  NC_000913/3385433‑3385728
                                                                                                                                                    |                                                                                                                                                   
cAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCtc                                                                                                                                                   >  2:1136948/1‑151 (MQ=255)
cAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCtc                                                                                                                                                   <  1:465354/151‑1 (MQ=255)
cAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCtc                                                                                                                                                   >  1:1645705/1‑151 (MQ=255)
cAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCtc                                                                                                                                                   >  1:164463/1‑151 (MQ=255)
    tAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGctct                                                                                                                                                  >  1:1703094/1‑148 (MQ=255)
    tAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCtctctc                                                                                                                                               >  2:530254/1‑151 (MQ=255)
     aGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCtctctc                                                                                                                                               <  1:58320/150‑1 (MQ=255)
      gcgcGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCtt                                                                                                                                             <  1:1315789/151‑1 (MQ=255)
       cgcgGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCttt                                                                                                                                            <  1:34456/151‑1 (MQ=255)
        gcgGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTc                                                                                                                                           >  1:249250/1‑151 (MQ=255)
                aattaGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCAtt                                                                                                                                   <  2:166097/151‑1 (MQ=255)
                aattaGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCAtt                                                                                                                                   >  1:992576/1‑151 (MQ=255)
                 attaGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTc                                                                                                                                  <  2:3919/151‑1 (MQ=255)
                  ttaGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCg                                                                                                                                 >  1:1051185/1‑151 (MQ=255)
                   taGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCg                                                                                                                                 <  2:93521/150‑1 (MQ=255)
                   taGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCg                                                                                                                                 <  2:1559980/150‑1 (MQ=255)
                   taGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGc                                                                                                                                >  2:926944/1‑151 (MQ=255)
                   taGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGc                                                                                                                                >  1:1516533/1‑151 (MQ=255)
                    aGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCt                                                                                                                               <  1:25833/151‑1 (MQ=255)
                        cAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGcc                                                                                                                            <  2:504216/150‑1 (MQ=255)
                        cAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCg                                                                                                                           <  2:1082446/151‑1 (MQ=255)
                             atTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTcc                                                                                                                      <  2:1140819/151‑1 (MQ=255)
                                aGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCAt                                                                                                                    <  1:477789/150‑1 (MQ=255)
                                aGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCAt                                                                                                                    <  1:313445/150‑1 (MQ=255)
                                aGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCAt                                                                                                                    <  2:1288868/150‑1 (MQ=255)
                                 gtgtATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTg                                                                                                                  <  1:1656890/151‑1 (MQ=255)
                                   gtATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGa                                                                                                                 <  1:551260/150‑1 (MQ=255)
                                    tataCTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGAtg                                                                                                               >  2:423188/1‑151 (MQ=255)
                                    tataCTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGAtg                                                                                                               >  1:1153609/1‑151 (MQ=255)
                                     ataCTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGAtg                                                                                                               <  1:81817/150‑1 (MQ=255)
                                       aCTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGAtgctg                                                                                                            >  1:664542/1‑151 (MQ=255)
                                           gATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTgcac                                                                                                         >  2:194742/1‑150 (MQ=255)
                                            aTTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTgcaca                                                                                                        >  2:1669138/1‑150 (MQ=255)
                                             ttttGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTgcaca                                                                                                        >  2:1382256/1‑149 (MQ=255)
                                                   gATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTgcacaagcacaa                                                                                                 >  1:567301/1‑150 (MQ=255)
                                                      atGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATc                                                                                             >  1:1107937/1‑151 (MQ=255)
                                                        gggTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGt                                                                                           <  2:1013042/151‑1 (MQ=255)
                                                            cACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGaa                                                                                        <  1:422424/150‑1 (MQ=255)
                                                            cACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGa                                                                                         >  1:874588/1‑149 (MQ=255)
                                                            cACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGa                                                                                         >  2:1532871/1‑149 (MQ=255)
                                                              cGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGc                                                                                      >  1:556232/1‑150 (MQ=255)
                                                                aaaCAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCaa                                                                                    <  1:1367164/150‑1 (MQ=255)
                                                                 aaCAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATc                                                                                  >  2:584226/1‑151 (MQ=255)
                                                                   cAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCgg                                                                                <  1:1236272/151‑1 (MQ=255)
                                                                   cAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCgg                                                                                <  2:455263/151‑1 (MQ=255)
                                                                         cccAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGt                                                                          >  2:828932/1‑151 (MQ=255)
                                                                            aGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATc                                                                       >  2:1347720/1‑151 (MQ=255)
                                                                            aGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATc                                                                       >  1:465744/1‑151 (MQ=255)
                                                                                aaaaGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGt                                                                    >  1:333144/1‑150 (MQ=255)
                                                                                  aaGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAg                                                                 <  1:1034400/151‑1 (MQ=255)
                                                                                    gATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAgtg                                                               <  2:731213/151‑1 (MQ=255)
                                                                                        aTGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAgtggtg                                                            <  2:298122/150‑1 (MQ=255)
                                                                                          gTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGtgtg                                                          <  1:333552/150‑1 (MQ=255)
                                                                                           tCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGc                                                        >  2:228423/1‑151 (MQ=255)
                                                                                            cGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCg                                                       <  2:1389095/151‑1 (MQ=255)
                                                                                             gAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGt                                                      >  2:561057/1‑151 (MQ=255)
                                                                                               ggTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCt                                                    <  1:40419/151‑1 (MQ=255)
                                                                                                gTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCtt                                                   <  2:688028/151‑1 (MQ=255)
                                                                                                  aaaaaTCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCg                                                 >  1:1640139/1‑151 (MQ=255)
                                                                                                  aaaaaTCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCg                                                 <  2:1002543/151‑1 (MQ=255)
                                                                                                  aaaaaTCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCg                                                 <  1:631691/151‑1 (MQ=255)
                                                                                                    aaaTCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGcc                                               <  2:1528486/151‑1 (MQ=255)
                                                                                                     aaTCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGcc                                               >  2:139494/1‑150 (MQ=255)
                                                                                                      aTCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCaa                                             <  2:78786/151‑1 (MQ=255)
                                                                                                          tGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATgg                                          >  1:1199236/1‑150 (MQ=255)
                                                                                                             aaaTCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGatat                                      <  2:1420974/151‑1 (MQ=255)
                                                                                                             aaaTCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGatat                                      <  2:194068/151‑1 (MQ=255)
                                                                                                              aaTCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATg                                     <  1:1137610/151‑1 (MQ=255)
                                                                                                               aTCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGc                                    >  1:1547852/1‑151 (MQ=255)
                                                                                                                 cAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGt                                  <  2:1581841/151‑1 (MQ=255)
                                                                                                                   aaaCCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTg                                 >  2:92032/1‑150 (MQ=255)
                                                                                                                   aaaCCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGa                                <  2:1454899/151‑1 (MQ=255)
                                                                                                                   aaaCCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGa                                <  2:1543973/151‑1 (MQ=255)
                                                                                                                    aaCCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGaa                               <  2:1102376/151‑1 (MQ=255)
                                                                                                                    aaCCACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGa                                >  1:701057/1‑150 (MQ=255)
                                                                                                                       cACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATg                            >  2:316396/1‑151 (MQ=255)
                                                                                                                       cACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATg                            >  2:667606/1‑151 (MQ=255)
                                                                                                                       cACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATg                            <  2:641034/151‑1 (MQ=255)
                                                                                                                       cACTGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATg                            >  1:550502/1‑151 (MQ=255)
                                                                                                                          tGTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTg                         >  2:1667124/1‑151 (MQ=255)
                                                                                                                           gTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCt                          <  2:1559013/149‑1 (MQ=255)
                                                                                                                           gTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTg                         <  2:1267881/150‑1 (MQ=255)
                                                                                                                           gTTGCTGCATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTg                         <  1:454235/150‑1 (MQ=255)
                                                                                                                               ctgcATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACa                     <  1:1323442/150‑1 (MQ=255)
                                                                                                                                 gcATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaaaa                  <  1:194728/151‑1 (MQ=255)
                                                                                                                                 gcATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaa                    <  2:628804/149‑1 (MQ=255)
                                                                                                                                  cATTAAGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaaaa                  >  1:653742/1‑150 (MQ=255)
                                                                                                                                       aGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCgg            <  1:527259/151‑1 (MQ=255)
                                                                                                                                       aGCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCgg            <  1:1136861/151‑1 (MQ=255)
                                                                                                                                        gCGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGa           <  2:1229964/151‑1 (MQ=255)
                                                                                                                                         cGTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGa           >  2:326825/1‑150 (MQ=255)
                                                                                                                                         cGTACTTTCTGCTCTCTCTTTCAGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGa           >  1:1536626/1‑150 (MQ=255)
                                                                                                                                          gTACTTTCTTCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGa           >  2:1255700/1‑149 (MQ=255)
                                                                                                                                          gTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAag         <  2:836657/151‑1 (MQ=255)
                                                                                                                                          gTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAag         <  1:1225164/151‑1 (MQ=255)
                                                                                                                                          gTACTTTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAag         <  2:197644/151‑1 (MQ=255)
                                                                                                                                          gTACTGTCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAag         <  2:1297536/151‑1 (MQ=255)
                                                                                                                                               ttCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGaa     <  2:1471144/150‑1 (MQ=255)
                                                                                                                                               ttCTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAACCAATCGGGACCGAATCCGTAAGTGGTGCGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGaa     <  1:596664/150‑1 (MQ=255)
                                                                                                                                                 cTGCTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAgg  >  2:453907/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTATGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGG  >  NC_000913/3385433‑3385728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: