Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,431,418 T→C intergenic (‑3/+2) tsaC ← / ← yrdD tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase/ssDNA‑binding protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,431,4180TC97.0% 377.3 / 1.0 100intergenic (‑3/+2)tsaC/yrdDtRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase/ssDNA‑binding protein, function unknown
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (49/48);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 103 total aligned reads displayed.

ACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACT  >  NC_000913/3431271‑3431565
                                                                                                                                                   |                                                                                                                                                   
aCTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGtcatt                                                                                                                                                  <  2:1051210/151‑1 (MQ=255)
   ccAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGtcatta                                                                                                                                                 <  1:341283/149‑1 (MQ=255)
   ccAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTcattat                                                                                                                                                <  1:842973/150‑1 (MQ=255)
      aCAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTccg                                                                                                                                            >  2:1216031/1‑151 (MQ=255)
       cAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTccgc                                                                                                                                           >  1:562807/1‑151 (MQ=255)
           cGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGa                                                                                                                                        <  1:992001/150‑1 (MQ=255)
            gCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGaaa                                                                                                                                      <  2:1280590/151‑1 (MQ=255)
             cATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAAc                                                                                                                                     >  1:1420982/1‑151 (MQ=255)
              aTCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAAcc                                                                                                                                    <  2:1382467/151‑1 (MQ=255)
               tCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCg                                                                                                                                   >  1:509327/1‑151 (MQ=255)
                 aCTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGc                                                                                                                                 >  1:1425250/1‑151 (MQ=255)
                    gctgTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCttt                                                                                                                              <  1:1702161/151‑1 (MQ=255)
                      tgTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTcc                                                                                                                            >  1:684293/1‑151 (MQ=255)
                       gTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTc                                                                                                                             <  2:1059933/149‑1 (MQ=255)
                        tttCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCcac                                                                                                                          >  2:58215/1‑151 (MQ=255)
                          tCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCcaca                                                                                                                         <  1:172022/150‑1 (MQ=255)
                          tCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCcaca                                                                                                                         <  1:120399/150‑1 (MQ=255)
                          tCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACAt                                                                                                                        >  2:1685133/1‑151 (MQ=255)
                            gCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACAtt                                                                                                                       >  1:646005/1‑150 (MQ=255)
                            gCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTg                                                                                                                      >  1:869652/1‑151 (MQ=255)
                                tCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGttt                                                                                                                   >  1:1056403/1‑150 (MQ=255)
                                  aGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACt                                                                                                                <  2:245894/151‑1 (MQ=255)
                                        gCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGcaca                                                                                                          <  1:1515428/151‑1 (MQ=255)
                                           cccAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa                                                                                                       <  1:126844/151‑1 (MQ=255)
                                           cccAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa                                                                                                       <  2:262291/151‑1 (MQ=255)
                                            ccAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAg                                                                                                      <  2:1030243/151‑1 (MQ=255)
                                            ccAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAg                                                                                                      <  1:630233/151‑1 (MQ=255)
                                             cAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgt                                                                                                     >  2:1549928/1‑151 (MQ=255)
                                             cAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgt                                                                                                     <  1:1566247/151‑1 (MQ=255)
                                              aaCACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgtg                                                                                                    <  1:1394629/151‑1 (MQ=255)
                                              aaCACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgtg                                                                                                    >  2:1247489/1‑151 (MQ=255)
                                               acacCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgtgt                                                                                                   >  1:882782/1‑151 (MQ=255)
                                               acacCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgtgt                                                                                                   <  1:973554/151‑1 (MQ=255)
                                                cacCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTTCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGtt                                                                                                  <  1:818916/151‑1 (MQ=255)
                                                 acCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGttt                                                                                                 >  2:318283/1‑151 (MQ=255)
                                                  ccGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGtttt                                                                                                <  1:1071251/151‑1 (MQ=255)
                                                     aaaaCGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTaca                                                                                             <  2:295427/151‑1 (MQ=255)
                                                      aaaCGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTacac                                                                                            >  1:366294/1‑151 (MQ=255)
                                                          ggCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTg                                                                                        >  1:682700/1‑151 (MQ=255)
                                                           gCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTgc                                                                                       >  1:1642415/1‑151 (MQ=255)
                                                              tCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTgcg                                                                                      <  2:1241522/149‑1 (MQ=255)
                                                               ccGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGcgcg                                                                                    <  1:715951/150‑1 (MQ=255)
                                                               ccGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCgg                                                                                   >  2:1700122/1‑151 (MQ=255)
                                                               ccGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCgg                                                                                   >  1:136657/1‑151 (MQ=255)
                                                                 gTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGtt                                                                                 <  1:1191488/151‑1 (MQ=255)
                                                                  ttGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttt                                                                                >  1:917964/1‑151 (MQ=255)
                                                                  ttGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttt                                                                                >  1:679942/1‑151 (MQ=255)
                                                                   tGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGtttt                                                                               >  2:433221/1‑151 (MQ=255)
                                                                    ggATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGtttt                                                                               <  2:1054833/150‑1 (MQ=255)
                                                                      aTAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttctt                                                                            >  1:588221/1‑151 (MQ=255)
                                                                        aGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttctttt                                                                          >  1:782637/1‑151 (MQ=255)
                                                                         ggCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttcttttc                                                                         <  1:1565689/151‑1 (MQ=255)
                                                                           cGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGa                                                                       <  1:1068634/151‑1 (MQ=255)
                                                                            gATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGAt                                                                      <  1:1232986/151‑1 (MQ=255)
                                                                             aTGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATg                                                                     <  1:1694258/151‑1 (MQ=255)
                                                                              tGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATg                                                                     >  1:216973/1‑150 (MQ=255)
                                                                                    gTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCg                                                              <  1:1191311/151‑1 (MQ=255)
                                                                                      tcttcATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCgg                                                             <  2:509378/150‑1 (MQ=255)
                                                                                      tcttcATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCgg                                                             >  1:323868/1‑150 (MQ=255)
                                                                                        ttcATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGAt                                                           >  2:795/1‑150 (MQ=255)
                                                                                         tcATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGAt                                                           <  2:1159773/149‑1 (MQ=255)
                                                                                         tcATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGAt                                                           <  2:361735/149‑1 (MQ=255)
                                                                                          cATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATaa                                                         <  1:388458/150‑1 (MQ=255)
                                                                                          cATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAAt                                                        >  2:502516/1‑151 (MQ=255)
                                                                                           aTTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATg                                                       <  1:685437/151‑1 (MQ=255)
                                                                                           aTTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATg                                                       <  2:917353/151‑1 (MQ=255)
                                                                                             tGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGa                                                      >  1:1047550/1‑150 (MQ=255)
                                                                                               agaACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGacac                                                   >  2:1181649/1‑151 (MQ=255)
                                                                                                  aCATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTc                                                 >  2:1109864/1‑150 (MQ=255)
                                                                                                   cATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAg                                               <  1:1356114/151‑1 (MQ=255)
                                                                                                      cTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGc                                            >  2:1626856/1‑151 (MQ=255)
                                                                                                        aTCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCAt                                          >  1:358812/1‑151 (MQ=255)
                                                                                                          cGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATtc                                        >  1:906719/1‑151 (MQ=255)
                                                                                                           gCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATtct                                       <  2:783968/151‑1 (MQ=255)
                                                                                                           gCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATtct                                       <  1:1681360/151‑1 (MQ=255)
                                                                                                             aGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTcc                                     >  2:1537096/1‑151 (MQ=255)
                                                                                                             aGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTcc                                     >  2:1716231/1‑151 (MQ=255)
                                                                                                               cTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTcc                                     >  1:1527276/1‑149 (MQ=255)
                                                                                                                   gATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTAt                               >  2:729925/1‑151 (MQ=255)
                                                                                                                   gATAGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTAt                               <  2:1592431/151‑1 (MQ=255)
                                                                                                                      aGCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATggg                            <  2:409999/151‑1 (MQ=255)
                                                                                                                       gCGTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGt                           <  1:1034148/151‑1 (MQ=255)
                                                                                                                         gTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGttt                         <  2:1421076/151‑1 (MQ=255)
                                                                                                                         gTCTCTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGttt                         <  1:1066335/151‑1 (MQ=255)
                                                                                                                          tctcTTTGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTg                        >  1:4296/1‑151 (MQ=255)
                                                                                                                              tttGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGt                    >  1:1607721/1‑151 (MQ=255)
                                                                                                                              tttGCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGt                    >  1:1390955/1‑151 (MQ=255)
                                                                                                                                 gCAGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTa                  <  1:1700006/150‑1 (MQ=255)
                                                                                                                                   aGGTTATTATTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATg               >  1:669576/1‑151 (MQ=255)
                                                                                                                                       tattatTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCa            <  1:25135/150‑1 (MQ=255)
                                                                                                                                       tattatTCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCa            >  2:639552/1‑150 (MQ=255)
                                                                                                                                        attattCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCaaa          <  1:1215938/151‑1 (MQ=255)
                                                                                                                                        attattCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCaa           <  2:668045/150‑1 (MQ=255)
                                                                                                                                          tattCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAAtt        >  1:1007395/1‑151 (MQ=255)
                                                                                                                                           attCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTg       <  1:1518725/151‑1 (MQ=255)
                                                                                                                                           attCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTg       >  1:1258631/1‑151 (MQ=255)
                                                                                                                                             tCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGa      <  2:1677856/150‑1 (MQ=255)
                                                                                                                                             tCACGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGa      <  1:383012/150‑1 (MQ=255)
                                                                                                                                               aCGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGaca    >  2:95562/1‑150 (MQ=255)
                                                                                                                                                cGTCATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACt  >  1:222638/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACT  >  NC_000913/3431271‑3431565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑