Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,447,416 T→C Y8C (TAC→TGC)  rplE ← 50S ribosomal subunit protein L5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,447,4160TC99.1% 443.6 / ‑4.3 114Y8C (TAC→TGC) rplE50S ribosomal subunit protein L5
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (65/48);  total (65/49)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

Only 100 of 115 total aligned reads displayed.

GGTCTGCTGCTGCGTTATCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACG  >  NC_000913/3447270‑3447561
                                                                                                                                                  |                                                                                                                                                 
ggTCTGCTGCTGCGTTATCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTgcagt                                                                                                                                                >  1:1285994/1‑150 (MQ=255)
    tgctgctgcGTTATCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATc                                                                                                                                            >  2:614731/1‑150 (MQ=255)
     gctgctgcGTTATCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATc                                                                                                                                            <  2:178330/149‑1 (MQ=255)
     gctgctgcGTTATCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCAt                                                                                                                                          <  1:684475/151‑1 (MQ=255)
     gctgctgcGTTATCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCAt                                                                                                                                          >  2:1331944/1‑151 (MQ=255)
     gctgctgcGTTATCCAGCAGTTTTTTGTCAGCGATCACTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATc                                                                                                                                            <  1:765247/149‑1 (MQ=255)
       tgctgcGTTATCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATg                                                                                                                                         >  2:475049/1‑150 (MQ=255)
           gcGTTATCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGtt                                                                                                                                    <  1:774114/151‑1 (MQ=255)
                aTCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGcc                                                                                                                               >  2:693079/1‑151 (MQ=255)
                 tCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGcc                                                                                                                               >  2:102768/1‑150 (MQ=255)
                    agcagTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCg                                                                                                                           >  1:797905/1‑151 (MQ=255)
                      cagTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGta                                                                                                                         <  1:1201697/151‑1 (MQ=255)
                      aagTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGt                                                                                                                          >  1:207893/2‑150 (MQ=255)
                       agTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTCTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGtac                                                                                                                        >  1:1618283/1‑151 (MQ=255)
                             ttGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTcc                                                                                                                  >  1:281734/1‑151 (MQ=255)
                                tCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCaa                                                                                                                >  2:9432/1‑150 (MQ=255)
                                tCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCaa                                                                                                                >  1:1054002/1‑150 (MQ=255)
                                 cAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAAt                                                                                                              >  1:1522394/1‑151 (MQ=255)
                                     gATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACt                                                                                                          <  1:1203586/151‑1 (MQ=255)
                                     gATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACt                                                                                                          >  1:940873/1‑151 (MQ=255)
                                      aTCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACtt                                                                                                         <  2:650105/151‑1 (MQ=255)
                                      aTCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACtt                                                                                                         <  1:326840/151‑1 (MQ=255)
                                      aTCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACtt                                                                                                         <  1:1539658/151‑1 (MQ=255)
                                          cTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGAt                                                                                                      >  1:763342/1‑150 (MQ=255)
                                          cTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGAt                                                                                                      >  1:162658/1‑150 (MQ=255)
                                               ccAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTc                                                                                                <  1:927226/151‑1 (MQ=255)
                                                  acacCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCt                                                                                             >  1:1458677/1‑151 (MQ=255)
                                                   cacCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTg                                                                                            <  2:454821/151‑1 (MQ=255)
                                                   cacCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTg                                                                                            <  1:759468/151‑1 (MQ=255)
                                                      ccATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGtt                                                                                          >  2:331561/1‑150 (MQ=255)
                                                      ccATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTa                                                                                         <  1:1187734/151‑1 (MQ=255)
                                                       cATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGtt                                                                                          >  1:1296712/1‑149 (MQ=255)
                                                          gTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACt                                                                                     <  2:1246179/151‑1 (MQ=255)
                                                          gTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACt                                                                                     <  2:1448128/151‑1 (MQ=255)
                                                             cAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTga                                                                                  <  1:1023594/151‑1 (MQ=255)
                                                              aGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTgaa                                                                                 <  2:1102507/151‑1 (MQ=255)
                                                                 gTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTgaagaa                                                                              <  2:1648014/151‑1 (MQ=255)
                                                                  tGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTgaagaa                                                                              >  2:545382/1‑150 (MQ=255)
                                                                  tGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTgaagaa                                                                              >  1:1222068/1‑150 (MQ=255)
                                                                  tGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTgaagaa                                                                              >  2:1575615/1‑150 (MQ=255)
                                                                        tctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACt                                                                       >  1:1266069/1‑151 (MQ=255)
                                                                         ctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACtt                                                                      >  1:81961/1‑151 (MQ=255)
                                                                           cGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACt                                                                       <  2:633339/148‑1 (MQ=255)
                                                                            gACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACttttt                                                                   >  1:1676505/1‑151 (MQ=255)
                                                                            gACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACttttt                                                                   >  2:828195/1‑151 (MQ=255)
                                                                             aCCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGACACGGACtttttt                                                                  >  1:271576/1‑151 (MQ=255)
                                                                              cccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACtttttt                                                                  >  2:225921/1‑150 (MQ=255)
                                                                                cGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTAcc                                                               <  1:1476004/151‑1 (MQ=255)
                                                                                 gAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCg                                                              >  1:901938/1‑151 (MQ=255)
                                                                                 gAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCg                                                              >  2:567414/1‑151 (MQ=255)
                                                                                    ggACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCt                                                           <  2:1593829/151‑1 (MQ=255)
                                                                                     gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCtt                                                          >  2:846533/1‑151 (MQ=255)
                                                                                      aCTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTc                                                         >  2:1283167/1‑151 (MQ=255)
                                                                                       cTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCg                                                        >  1:1004995/1‑151 (MQ=255)
                                                                                         tGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGa                                                       >  1:1456085/1‑150 (MQ=255)
                                                                                          gCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGaa                                                      <  2:1662018/150‑1 (MQ=255)
                                                                                          gCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAAt                                                     <  1:415588/151‑1 (MQ=255)
                                                                                            aTGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCt                                                   >  1:281271/1‑151 (MQ=255)
                                                                                              gACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaa                                                 <  2:1467969/151‑1 (MQ=255)
                                                                                               aCAGAATTGTAGTTAAACTAAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaa                                                 >  1:1696148/1‑150 (MQ=255)
                                                                                                  gAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGcc                                             >  2:804781/1‑151 (MQ=255)
                                                                                                  gAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGcc                                             >  1:939552/1‑151 (MQ=255)
                                                                                                    aTTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCt                                            >  1:934869/1‑150 (MQ=255)
                                                                                                    aTTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTa                                           <  1:589393/151‑1 (MQ=255)
                                                                                                    aTTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTa                                           <  1:1504800/151‑1 (MQ=255)
                                                                                                     ttGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTa                                           >  2:473408/1‑150 (MQ=255)
                                                                                                      tGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCACCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTac                                          >  2:231561/1‑150 (MQ=255)
                                                                                                        tAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACg                                       >  2:1355273/1‑151 (MQ=255)
                                                                                                         aGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACgg                                      <  2:869080/151‑1 (MQ=255)
                                                                                                             aaaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTc                                    >  1:1163689/1‑149 (MQ=255)
                                                                                                             aaaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCa                                   <  2:1144825/150‑1 (MQ=255)
                                                                                                                 tCAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCtt                              >  1:347271/1‑151 (MQ=255)
                                                                                                                  cAGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCt                               <  2:1286087/149‑1 (MQ=255)
                                                                                                                   aGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                             >  2:701761/1‑150 (MQ=255)
                                                                                                                   aGTCATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGc                            >  2:47450/1‑151 (MQ=255)
                                                                                                                      cATGAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCg                          >  2:485696/1‑150 (MQ=255)
                                                                                                                         gAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGtt                       <  2:233720/150‑1 (MQ=255)
                                                                                                                         gAGTTTTTTAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTg                      >  2:951413/1‑151 (MQ=255)
                                                                                                                             tttttAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGc                  >  1:393944/1‑151 (MQ=255)
                                                                                                                                 tAACTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCAtt               <  2:1617418/150‑1 (MQ=255)
                                                                                                                                  aaCTACTTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTg              >  2:1113923/1‑150 (MQ=255)
                                                                                                                                   actactTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGaa            <  2:191718/151‑1 (MQ=255)
                                                                                                                                    ctactTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGa             >  1:146004/1‑149 (MQ=255)
                                                                                                                                    ctactTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGa             >  2:740904/1‑149 (MQ=255)
                                                                                                                                     tactTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGa             >  1:1508794/1‑148 (MQ=255)
                                                                                                                                     tactTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGa             >  1:443879/1‑148 (MQ=255)
                                                                                                                                      actTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGa             >  1:479561/1‑147 (MQ=255)
                                                                                                                                      actTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAg           <  2:1611604/149‑1 (MQ=255)
                                                                                                                                      actTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAg           <  2:754549/149‑1 (MQ=255)
                                                                                                                                      actTCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGa          <  2:109595/150‑1 (MQ=255)
                                                                                                                                       ctTCGTCTTTGCAGTAATCATGCAGTTTTGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGGCTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAt         <  1:1331853/150‑1 (MQ=255)
                                                                                                                                         tCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAtt        <  1:1290967/149‑1 (MQ=255)
                                                                                                                                         tCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAtt        <  1:51641/149‑1 (MQ=255)
                                                                                                                                         tCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTg       <  1:479588/150‑1 (MQ=255)
                                                                                                                                         tCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGc      >  1:925514/1‑151 (MQ=255)
                                                                                                                                         tCGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGc      >  1:1281823/1‑151 (MQ=255)
                                                                                                                                          cGTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGc      <  2:1054081/150‑1 (MQ=255)
                                                                                                                                           gTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTa    <  1:806775/151‑1 (MQ=255)
                                                                                                                                           gTCTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTa    <  2:1360835/151‑1 (MQ=255)
                                                                                                                                             cTTTGCAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACg  >  1:932677/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGTCTGCTGCTGCGTTATCCAGCAGTTTTTTGTCAGCGATCGCTTCACCAACACCCATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACG  >  NC_000913/3447270‑3447561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: