Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,546,863 T→C V102A (GTA→GCA)  gntT → gluconate transporter, high‑affinity GNT I system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,546,8630TC97.2% 265.4 / ‑1.6 72V102A (GTA→GCA) gntTgluconate transporter, high‑affinity GNT I system
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (39/31);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01

GTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCT  >  NC_000913/3546718‑3547002
                                                                                                                                                 |                                                                                                                                           
gtCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACtt                                                                                                                                         <  1:1023042/150‑2 (MQ=255)
gtCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTg                                                                                                                                         >  2:394690/1‑150 (MQ=255)
gtCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTg                                                                                                                                         >  1:1433760/1‑150 (MQ=255)
   ggcggGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCg                                                                                                                                     >  1:35731/1‑151 (MQ=255)
     cggGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGt                                                                                                                                   >  1:517283/1‑151 (MQ=255)
      gggACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGtt                                                                                                                                  >  2:799887/1‑151 (MQ=255)
       ggACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGttt                                                                                                                                 <  1:308159/151‑1 (MQ=255)
           gCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTAcc                                                                                                                             >  2:857391/1‑151 (MQ=255)
             tCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGt                                                                                                                           >  1:74653/1‑151 (MQ=255)
                  aGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGt                                                                                                                       <  2:919683/150‑1 (MQ=255)
                  aGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGt                                                                                                                       <  2:1074160/150‑1 (MQ=255)
                   gCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGttt                                                                                                                     <  1:537719/151‑1 (MQ=255)
                   gCCTTGCCCTGATCATGGATTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGttt                                                                                                                     <  1:846418/151‑1 (MQ=255)
                    ccTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGtttt                                                                                                                    <  2:154736/151‑1 (MQ=255)
                       tGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGc                                                                                                                  <  1:1193285/150‑1 (MQ=255)
                          ccTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTg                                                                                                              >  2:207625/1‑151 (MQ=255)
                             gATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTc                                                                                                           >  2:1588566/1‑151 (MQ=255)
                               tCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCt                                                                                                          >  2:1380430/1‑150 (MQ=255)
                                 aTGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATg                                                                                                       >  1:971193/1‑151 (MQ=255)
                                  tGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATg                                                                                                       >  2:1311228/1‑150 (MQ=255)
                                   gggTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGaa                                                                                                     <  1:750072/151‑1 (MQ=255)
                                    ggTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAATGCGGTGTCGCACAACGTATCGCCACCACGCTGATTGCCAAAGTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAg                                                                                                    <  2:1486430/151‑1 (MQ=255)
                                      ttttGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTg                                                                                                  >  2:1221523/1‑151 (MQ=255)
                                            cgcAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCttt                                                                                            >  1:1305128/1‑151 (MQ=255)
                                               aaTGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTtgtg                                                                                         >  1:187472/1‑151 (MQ=255)
                                                aTGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGc                                                                                        >  1:262709/1‑151 (MQ=255)
                                                  gCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCt                                                                                       <  2:156629/150‑1 (MQ=255)
                                                  gCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTg                                                                                      >  2:1160930/1‑151 (MQ=255)
                                                  gCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTg                                                                                      >  2:953413/1‑151 (MQ=255)
                                                        cAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGAtgctg                                                                                <  2:44669/151‑1 (MQ=255)
                                                                 ggCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTgg                                                                         <  2:452339/149‑1 (MQ=255)
                                                                 ggCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTgg                                                                         <  1:1311134/149‑1 (MQ=255)
                                                                  gCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGgtgt                                                                      >  2:733977/1‑151 (MQ=255)
                                                                   aaGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGtt                                                                     >  2:1515613/2‑151 (MQ=255)
                                                                    aGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGttt                                                                    <  2:1352912/151‑1 (MQ=255)
                                                                      aCTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTAc                                                                  <  2:90359/151‑1 (MQ=255)
                                                                         gCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCa                                                                <  2:872496/150‑1 (MQ=255)
                                                                          cGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATc                                                              <  1:459204/151‑1 (MQ=255)
                                                                              ggCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCgg                                                          <  1:445457/151‑1 (MQ=255)
                                                                                  cacaACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTc                                                      >  2:1269938/1‑151 (MQ=255)
                                                                                   acaACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCt                                                     >  2:1377977/1‑151 (MQ=255)
                                                                                   acaACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCt                                                     >  1:1390782/1‑151 (MQ=255)
                                                                                     aaCGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGc                                                   <  2:922596/151‑1 (MQ=255)
                                                                                         tATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAat                                               >  2:941648/1‑151 (MQ=255)
                                                                                          aTCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAat                                               <  1:720062/150‑1 (MQ=255)
                                                                                           tCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAatat                                             >  2:793633/1‑151 (MQ=255)
                                                                                            cGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATAtt                                            <  2:93660/151‑1 (MQ=255)
                                                                                               caccacGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTcc                                          >  1:1116331/1‑150 (MQ=255)
                                                                                                 ccacGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCAct                                       <  1:530732/151‑1 (MQ=255)
                                                                                                  cacGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCActg                                      <  2:48045/151‑1 (MQ=255)
                                                                                                    cGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCActgct                                    >  2:616941/1‑151 (MQ=255)
                                                                                                        gATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTAt                                >  2:1500838/1‑151 (MQ=255)
                                                                                                          ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGt                              >  1:1106461/1‑151 (MQ=255)
                                                                                                          ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGt                              >  1:1172399/1‑151 (MQ=255)
                                                                                                           tGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGt                              <  2:1607119/150‑1 (MQ=255)
                                                                                                             ccAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTgg                           <  2:704439/151‑1 (MQ=255)
                                                                                                              cAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGgt                          <  1:971328/151‑1 (MQ=255)
                                                                                                                aaTTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGgtgt                        <  2:74656/151‑1 (MQ=255)
                                                                                                                 aTTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGgtgt                        >  2:1431883/1‑150 (MQ=255)
                                                                                                                 aTTTGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTa                       <  2:42719/151‑1 (MQ=255)
                                                                                                                    tGGTAAAAAACACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTAcc                     <  2:122334/150‑1 (MQ=255)
                                                                                                                         aaaaaCACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggc               >  1:110206/1‑151 (MQ=255)
                                                                                                                          aaaaCACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcg              >  2:87715/1‑151 (MQ=255)
                                                                                                                           aaaCACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcgg             >  1:817936/1‑151 (MQ=255)
                                                                                                                           aaaCACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcgg             >  2:1147779/1‑151 (MQ=255)
                                                                                                                            aaCACATCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcggc            >  2:1488636/1‑151 (MQ=255)
                                                                                                                               acaTCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGc         >  2:1513276/1‑151 (MQ=255)
                                                                                                                                caTCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGc         >  1:415595/1‑150 (MQ=255)
                                                                                                                                  tCCAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCAct      >  2:1104222/1‑151 (MQ=255)
                                                                                                                                    cAGTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCActgt    <  2:1543121/151‑1 (MQ=255)
                                                                                                                                      gTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCActgtct  <  1:394662/151‑1 (MQ=255)
                                                                                                                                      gTGGGCGGTGGCACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCActgtct  <  2:35732/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
GTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCT  >  NC_000913/3546718‑3547002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: