Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,632,618 G→A intergenic (‑28/+234) yhiJ ← / ← yhiL DUF4049 family protein/pseudogene, DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,632,6180GA99.1% 465.3 / ‑4.5 115intergenic (‑28/+234)yhiJ/yhiLDUF4049 family protein/pseudogene, DUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (46/68);  total (47/68)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 116 total aligned reads displayed.

ATTTGCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGT  >  NC_000913/3632474‑3632765
                                                                                                                                                |                                                                                                                                                   
aTTTGCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACa                                                                                                                                               <  2:1359912/151‑1 (MQ=255)
  ttGCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACAt                                                                                                                                              <  2:869090/150‑1 (MQ=255)
   tGCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACAt                                                                                                                                              >  1:53925/1‑149 (MQ=255)
    gCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAg                                                                                                                                            <  2:136749/150‑1 (MQ=255)
     cAATATACCCAAACAGTTCTTTAGTGACGTTGGTAACATGCTCTTGCACCATATCATTTGTTTCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTTCCTCATATTATTTTCCGCGAGAAATACATAGtt                                                                                                                                          <  1:761743/151‑1 (MQ=255)
     cAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGtt                                                                                                                                          <  1:1132163/151‑1 (MQ=255)
       atatACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTa                                                                                                                                         >  1:986258/1‑150 (MQ=255)
         ataCCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAAc                                                                                                                                       >  1:1098638/1‑150 (MQ=255)
         ataCCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACa                                                                                                                                      <  2:1285586/151‑1 (MQ=255)
           aCCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACAtt                                                                                                                                    >  1:182051/1‑151 (MQ=255)
            cccAAACCGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACAttt                                                                                                                                   >  1:1629541/1‑151 (MQ=255)
            cccAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACAttt                                                                                                                                   >  2:1511230/1‑151 (MQ=255)
            cccAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACAtt                                                                                                                                    <  1:497385/150‑1 (MQ=255)
              cAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACAtttt                                                                                                                                  >  1:399685/1‑150 (MQ=255)
              cAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTa                                                                                                                                 <  1:949598/151‑1 (MQ=255)
                 aCAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCt                                                                                                                              <  1:284672/151‑1 (MQ=255)
                 aCAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCt                                                                                                                              >  2:354218/1‑151 (MQ=255)
                  cAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCtt                                                                                                                             <  2:536024/151‑1 (MQ=255)
                   aGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTa                                                                                                                            <  1:1332537/151‑1 (MQ=255)
                     ttCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTAt                                                                                                                           <  1:1633061/150‑1 (MQ=255)
                      tCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATg                                                                                                                          >  1:1440579/1‑150 (MQ=255)
                       cTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGAc                                                                                                                        >  1:313790/1‑151 (MQ=255)
                        tttAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGAc                                                                                                                        >  2:1451469/1‑150 (MQ=255)
                             tGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATaca                                                                                                                   <  1:418505/150‑1 (MQ=255)
                                   tGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCAt                                                                                                             <  1:1212662/150‑1 (MQ=255)
                                       aaCATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTc                                                                                                         <  2:1180094/150‑1 (MQ=255)
                                       aaCATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCg                                                                                                        <  1:1446060/151‑1 (MQ=255)
                                       aaCATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCg                                                                                                        >  1:7411/1‑151 (MQ=255)
                                       aaCATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCg                                                                                                        <  2:1552561/151‑1 (MQ=255)
                                        aCATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGa                                                                                                       <  2:653954/151‑1 (MQ=255)
                                         cATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGAt                                                                                                      >  1:839070/1‑151 (MQ=255)
                                          aTGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATg                                                                                                     <  1:254392/151‑1 (MQ=255)
                                            gCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATgg                                                                                                    <  2:1621362/150‑1 (MQ=255)
                                               ctTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGa                                                                                                  <  1:1414670/149‑1 (MQ=255)
                                               ctTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGa                                                                                                  <  2:402361/149‑1 (MQ=255)
                                                ttGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGaaa                                                                                                >  1:1551141/1‑150 (MQ=255)
                                                 tGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGaaaa                                                                                               <  2:983889/150‑1 (MQ=255)
                                                 tGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGaaa                                                                                                <  2:1373215/149‑1 (MQ=255)
                                                 tGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGaaa                                                                                                <  1:544690/149‑1 (MQ=255)
                                                  gCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAaat                                                                                              <  2:218416/150‑1 (MQ=255)
                                                     ccATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAAtt                                                                                          <  1:279070/151‑1 (MQ=255)
                                                       atatCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATtat                                                                                        >  1:446820/1‑151 (MQ=255)
                                                       atatCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATtat                                                                                        <  2:371080/151‑1 (MQ=255)
                                                       atatCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATtat                                                                                        <  1:665306/151‑1 (MQ=255)
                                                        tatCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATtat                                                                                        >  1:1012812/1‑150 (MQ=255)
                                                         atCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTatat                                                                                      >  2:880493/1‑151 (MQ=255)
                                                         atCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTatat                                                                                      >  1:158705/1‑151 (MQ=255)
                                                          tCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCATATGACATACACCTCATGTTCGATGGGAAAATAATTATAtt                                                                                     >  1:1334276/1‑151 (MQ=255)
                                                           cATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATAttt                                                                                    >  2:12426/1‑151 (MQ=255)
                                                               tgttgCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGc                                                                                 <  2:1674523/150‑1 (MQ=255)
                                                                  tgCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGcaca                                                                              >  1:693674/1‑150 (MQ=255)
                                                                  tgCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGcaca                                                                              <  1:91127/150‑1 (MQ=255)
                                                                   gCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACaa                                                                             >  2:1490351/1‑150 (MQ=255)
                                                                   gCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACaa                                                                             <  2:460023/150‑1 (MQ=255)
                                                                    cGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAAt                                                                           >  2:766087/1‑151 (MQ=255)
                                                                    cGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAAt                                                                           <  1:1462211/151‑1 (MQ=255)
                                                                     gATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATc                                                                          >  1:1346486/1‑151 (MQ=255)
                                                                         gTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTc                                                                      <  2:1338297/151‑1 (MQ=255)
                                                                            gTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGc                                                                   >  2:629353/1‑151 (MQ=255)
                                                                             ttGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGcc                                                                  >  2:822500/1‑151 (MQ=255)
                                                                             ttGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGcc                                                                  >  1:1102557/1‑151 (MQ=255)
                                                                                 aCTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCa                                                               <  1:1402883/150‑1 (MQ=255)
                                                                                  cTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCaa                                                              <  2:390995/150‑1 (MQ=255)
                                                                                    gTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCaaa                                                             <  1:1353255/149‑1 (MQ=255)
                                                                                    gTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCaaa                                                             <  2:471661/149‑1 (MQ=255)
                                                                                    gTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGc                                                           >  2:661967/1‑151 (MQ=255)
                                                                                     tCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGc                                                           <  1:528076/150‑1 (MQ=255)
                                                                                       gTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCat                                                         >  1:1037981/1‑150 (MQ=255)
                                                                                          aGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCataata                                                     <  1:1638123/151‑1 (MQ=255)
                                                                                          aGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCataat                                                      >  2:115712/1‑150 (MQ=255)
                                                                                              cctgccACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGaa                                                 <  1:1425157/151‑1 (MQ=255)
                                                                                               ctgccACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGaa                                                 >  1:172349/1‑150 (MQ=255)
                                                                                                 gccACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAAt                                               <  1:927646/150‑1 (MQ=255)
                                                                                                 gccACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAAt                                               <  1:171551/150‑1 (MQ=255)
                                                                                                  ccACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAAttt                                             <  1:100738/151‑1 (MQ=255)
                                                                                                    acaGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCt                                           <  1:526633/151‑1 (MQ=255)
                                                                                                     caGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCtt                                          >  2:529197/1‑151 (MQ=255)
                                                                                                      aGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTa                                         <  1:661908/151‑1 (MQ=255)
                                                                                                       gTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAGTTTCTTAt                                        <  2:53930/151‑1 (MQ=255)
                                                                                                        ttCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTAtc                                       >  2:1524198/1‑151 (MQ=255)
                                                                                                        ttCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTAtc                                       >  2:1355363/1‑151 (MQ=255)
                                                                                                         tCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTAtct                                      >  1:516833/1‑151 (MQ=255)
                                                                                                         tCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTAtct                                      >  2:233790/1‑151 (MQ=255)
                                                                                                          ccAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTAtct                                      <  1:330219/150‑1 (MQ=255)
                                                                                                           cAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAACGCATAATATGAAATTTCTTAtct                                      >  2:196968/1‑149 (MQ=255)
                                                                                                           cAATTTTCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTAtctc                                     >  1:1280542/1‑150 (MQ=255)
                                                                                                               tttCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAg                                 <  1:590102/150‑1 (MQ=255)
                                                                                                               tttCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGc                                >  2:1440851/1‑151 (MQ=255)
                                                                                                                  cATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCaga                             <  2:182064/151‑1 (MQ=255)
                                                                                                                      ttttACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGaa                          >  2:874253/1‑150 (MQ=255)
                                                                                                                        ttACCTCATATTATTTATCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAg                        <  2:1152427/150‑1 (MQ=255)
                                                                                                                          aCCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTcc                     >  1:1692778/1‑151 (MQ=255)
                                                                                                                          aCCTCATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTcc                     <  1:822434/151‑1 (MQ=255)
                                                                                                                              cATATTATTTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTa                  >  1:1445180/1‑150 (MQ=255)
                                                                                                                                 attattTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAg               <  2:1234310/150‑1 (MQ=255)
                                                                                                                                   tattTACCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGAt             <  2:1346579/150‑1 (MQ=255)
                                                                                                                                        aCCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGt          >  2:1625026/1‑148 (MQ=255)
                                                                                                                                        aCCGCGAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTag        <  1:629295/150‑1 (MQ=255)
                                                                                                                                           gcgAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGc    >  1:490169/1‑151 (MQ=255)
                                                                                                                                            cgAGAAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGc    <  1:1254515/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATTTGCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGT  >  NC_000913/3632474‑3632765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: