Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,788,944	T→C	F282L (TTT→CTT)	yibQ →	putative polysaccharide deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,788,944	0	T	C	100.0%	337.4 / NA	85	F282L (TTT→CTT)	yibQ	putative polysaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/47);  total (38/47)

AAAGCCAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCG  >  NC_000913/3788797‑3789091                                                                                                                                                    |                                                                                                                                                    aaaGCCAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCctt                                                                                                                                                   <  1:13946/150‑1 (MQ=255) aaaGCCAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCctt                                                                                                                                                   >  1:983110/1‑150 (MQ=255) aaaGCCAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTg                                                                                                                                                  <  1:902305/151‑1 (MQ=255)   aGCCAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGaa                                                                                                                                                <  1:1260310/151‑1 (MQ=255)   aGCCAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGa                                                                                                                                                 >  2:1255717/1‑150 (MQ=255)    gCCAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGaa                                                                                                                                                <  2:569156/150‑1 (MQ=255)    gCCAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGa                                                                                                                                                 >  1:367426/1‑149 (MQ=255)     ccAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGt                                                                                                                                              <  1:744074/151‑1 (MQ=255)     ccAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGt                                                                                                                                              >  2:1537342/1‑151 (MQ=255)      cagcagCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGt                                                                                                                                              >  1:1485851/1‑150 (MQ=255)       agcagcTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTAt                                                                                                                                            >  2:1695063/1‑151 (MQ=255)        gcagcTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTAtt                                                                                                                                           <  1:890914/151‑1 (MQ=255)        gcagcTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTAt                                                                                                                                            <  1:1587117/150‑1 (MQ=255)          agcTTGCTGAATGAACCGCAGGTTGATACTTCTCCACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCGTGAAGTAGTaa                                                                                                                                         <  1:356004/151‑1 (MQ=255)          agcTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTaa                                                                                                                                         <  1:929747/151‑1 (MQ=255)           gcTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAg                                                                                                                                        <  2:888669/151‑1 (MQ=255)           gcTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGAATTa                                                                                                                                          >  1:1391943/1‑149 (MQ=255)           gatttcTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTAtt                                                                                                                                           <  2:1379161/143‑1 (MQ=255)              tGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGc                                                                                                                                       <  2:1529885/149‑1 (MQ=255)               gCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGaa                                                                                                                                    <  2:1083831/151‑1 (MQ=255)                 tgaatgaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGaa                                                                                                                                    >  2:1639683/1‑149 (MQ=255)                 tgaatgaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATAGCTTCCTTGAAGTATTAAGCGaa                                                                                                                                    >  1:1072737/1‑149 (MQ=255)                  gaatgaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGaaa                                                                                                                                   <  2:449223/149‑1 (MQ=255)                  gaatgaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAg                                                                                                                                  <  1:498323/150‑1 (MQ=255)                    atgaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCAt                                                                                                                               >  2:735828/1‑151 (MQ=255)                      gaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATc                                                                                                                              >  2:1012497/1‑150 (MQ=255)                      gaaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATc                                                                                                                              >  1:659438/1‑150 (MQ=255)                       aaCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATc                                                                                                                              >  2:564312/1‑149 (MQ=255)                        aCCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGc                                                                                                                           >  1:1192106/1‑151 (MQ=255)                         ccGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGcc                                                                                                                          <  2:1480532/151‑1 (MQ=255)                              ggTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGc                                                                                                                     <  2:206986/151‑1 (MQ=255)                                ttGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCAc                                                                                                                   >  2:204975/1‑151 (MQ=255)                                  gATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGc                                                                                                                 >  2:1643033/1‑151 (MQ=255)                                     aCTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTg                                                                                                               <  2:1199949/150‑1 (MQ=255)                                        tCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGAt                                                                                                             <  2:812165/149‑1 (MQ=255)                                        tCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGAt                                                                                                             <  1:1537244/149‑1 (MQ=255)                                        tCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATc                                                                                                            <  1:779558/150‑1 (MQ=255)                                        tCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCg                                                                                                           >  1:1165247/1‑151 (MQ=255)                                          tACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGtt                                                                                                         >  1:1596304/1‑151 (MQ=255)                                           acacCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGtt                                                                                                         <  1:1223918/150‑1 (MQ=255)                                           acacCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGtt                                                                                                         <  2:1257187/150‑1 (MQ=255)                                            cacCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGttt                                                                                                        >  1:1028170/1‑150 (MQ=255)                                            cacCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTa                                                                                                       <  2:186104/151‑1 (MQ=255)                                                gccgAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTc                                                                                                   >  2:40918/1‑151 (MQ=255)                                                  cgAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCa                                                                                                 <  1:1085501/151‑1 (MQ=255)                                                    aaaaaCGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGc                                                                                               <  1:101030/151‑1 (MQ=255)                                                     aaaaCGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGc                                                                                               >  1:1112720/1‑150 (MQ=255)                                                     aaaaCGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGc                                                                                               >  2:231249/1‑150 (MQ=255)                                                        aCGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCa                                                                                           <  1:1634238/151‑1 (MQ=255)                                                             gtgCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTg                                                                                        <  1:1676422/149‑1 (MQ=255)                                                                   gATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCt                                                                                <  1:486982/151‑1 (MQ=255)                                                                          cgcgTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCaa                                                                         <  2:63113/151‑1 (MQ=255)                                                                             gTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAAc                                                                       >  1:1664668/1‑150 (MQ=255)                                                                             gTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACa                                                                      <  1:943781/151‑1 (MQ=255)                                                                                  ccgttccgtGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGccc                                                                  <  1:983965/150‑1 (MQ=255)                                                                                  ccgttccgtGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCg                                                                 <  2:308777/151‑1 (MQ=255)                                                                                   cgttccgtGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCg                                                                 >  2:1520217/1‑150 (MQ=255)                                                                                    gttccgtGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGaa                                                               <  2:784482/151‑1 (MQ=255)                                                                                      tccgtGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAgc                                                             >  1:663750/1‑151 (MQ=255)                                                                                         gtGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAgcggc                                                          <  2:932195/151‑1 (MQ=255)                                                                                           ggCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAgcggcg                                                         <  1:1305523/150‑1 (MQ=255)                                                                                            gCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGaa                                                       <  1:904748/151‑1 (MQ=255)                                                                                            gCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGaa                                                       <  1:216214/151‑1 (MQ=255)                                                                                                gAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGttt                                                   <  1:1214957/151‑1 (MQ=255)                                                                                                gAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGtt                                                    <  2:1652083/150‑1 (MQ=255)                                                                                                 aaGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGttt                                                   >  1:1593204/1‑150 (MQ=255)                                                                                                      gtgCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCt                                             <  2:437437/151‑1 (MQ=255)                                                                                                        gCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCt                                             >  1:378292/1‑149 (MQ=255)                                                                                                         cAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAgc                                          <  2:1064174/151‑1 (MQ=255)                                                                                                           aaCCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAgcgc                                        >  2:879749/1‑151 (MQ=255)                                                                                                            aCCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCa                                       <  1:327836/151‑1 (MQ=255)                                                                                                                aagaaACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAAtt                                   <  2:416091/151‑1 (MQ=255)                                                                                                                  gaaACCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTa                                  >  2:1083381/1‑150 (MQ=255)                                                                                                                    aaCCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTaa                                 >  1:132322/1‑149 (MQ=255)                                                                                                                    aaCCGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAg                               <  2:983187/151‑1 (MQ=255)                                                                                                                      ccGATAGAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAgg                              >  2:1463530/1‑150 (MQ=255)                                                                                                                            gAACCTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTAcg                       <  1:1255621/151‑1 (MQ=255)                                                                                                                               ccTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGt                    <  2:1174580/151‑1 (MQ=255)                                                                                                                               ccTGTTTATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGt                    >  2:1154635/1‑151 (MQ=255)                                                                                                                                    ttATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATg                >  2:680008/1‑150 (MQ=255)                                                                                                                                    ttATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATg                >  2:1025949/1‑150 (MQ=255)                                                                                                                                     tATGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGc               <  2:1664779/150‑1 (MQ=255)                                                                                                                                      aTGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCg              >  1:168395/1‑150 (MQ=255)                                                                                                                                       tGCTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGAt            >  2:717403/1‑151 (MQ=255)                                                                                                                                         cTAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATgg          >  1:1237248/1‑151 (MQ=255)                                                                                                                                          tAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGt         >  2:1632153/1‑151 (MQ=255)                                                                                                                                          tAATCGCTTCCTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGt         >  2:766359/1‑151 (MQ=255)                                                                                                                                                 ttcctTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg  >  2:1126876/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    AAAGCCAGCAGCTTGCTGAATGAACCGCAGGTTGATACTTCTACACCGCCGAAAAACGCTGTGCCTGATGCACCGCGTAATCCGTTCCGTGGCGTGAAGCTGTGCAAACCGAAGAAACCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCG  >  NC_000913/3788797‑3789091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑