Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,904,425 A→G A391A (GCT→GCC bglF ← fused beta‑glucoside‑specific PTS enzymes: IIA component/IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,904,4250AG98.9% 346.6 / ‑3.8 93A391A (GCT→GCCbglFfused beta‑glucoside‑specific PTS enzymes: IIA component/IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (43/49);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

ACCGATGGCAATGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACG  >  NC_000913/3904278‑3904572
                                                                                                                                                   |                                                                                                                                                   
aCCGATGGCAATGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGcc                                                                                                                                                  >  2:659978/1‑151 (MQ=255)
   gATGGCAATGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGcccc                                                                                                                                                >  2:921189/1‑150 (MQ=255)
   gATGGCAATGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGcccc                                                                                                                                                >  1:1615983/1‑150 (MQ=255)
         aaTGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGa                                                                                                                                           <  2:1480244/149‑1 (MQ=255)
         aaTGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGAt                                                                                                                                          <  1:1261555/150‑1 (MQ=255)
           tGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATAc                                                                                                                                        >  2:594620/1‑150 (MQ=255)
                ccGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATAc                                                                                                                                        <  2:200317/145‑1 (MQ=255)
                 cGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCTAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGAt                                                                                                                                 <  1:1222431/151‑1 (MQ=255)
                 cGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGAt                                                                                                                                 <  1:687221/151‑1 (MQ=255)
                 cGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGAt                                                                                                                                 >  1:1654313/1‑151 (MQ=255)
                    cAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAAc                                                                                                                              >  2:991213/1‑151 (MQ=255)
                    cAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAAc                                                                                                                              >  2:1102743/1‑151 (MQ=255)
                       tAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACaa                                                                                                                            >  1:1621309/1‑150 (MQ=255)
                        aaCGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACaaa                                                                                                                           <  1:199893/150‑1 (MQ=255)
                        aaCGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACaaa                                                                                                                           <  2:1469612/150‑1 (MQ=255)
                          cGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAggg                                                                                                                        <  1:1382493/151‑1 (MQ=255)
                          cGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAggg                                                                                                                        <  2:1678918/151‑1 (MQ=255)
                           gCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAgggg                                                                                                                       >  1:198052/1‑151 (MQ=255)
                           gCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAgggg                                                                                                                       <  2:379796/151‑1 (MQ=255)
                                 ccAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACtt                                                                                                                  >  2:1312970/1‑150 (MQ=255)
                                 ccAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTa                                                                                                                 <  2:1337320/151‑1 (MQ=255)
                                  cAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTAc                                                                                                                >  2:775726/1‑151 (MQ=255)
                                   aGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTAcg                                                                                                               >  1:13739/1‑151 (MQ=255)
                                   aGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTAcg                                                                                                               <  1:1558976/151‑1 (MQ=255)
                                   aGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTAcg                                                                                                               <  2:410233/151‑1 (MQ=255)
                                     aCGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTAcgcg                                                                                                             <  2:1540405/151‑1 (MQ=255)
                                      cGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCgg                                                                                                            >  1:839247/1‑151 (MQ=255)
                                       tgTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGc                                                                                                           >  1:580426/2‑151 (MQ=255)
                                       ggTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGc                                                                                                           <  1:232379/151‑1 (MQ=255)
                                          gAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAgg                                                                                                        <  1:1209157/151‑1 (MQ=255)
                                          gAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAg                                                                                                         <  2:651480/150‑1 (MQ=255)
                                            aaTCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGtt                                                                                                      <  2:1047059/151‑1 (MQ=255)
                                             aTCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTAc                                                                                                                >  1:986128/1‑140 (MQ=255)
                                             aTCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTAc                                                                                                                <  2:986205/140‑1 (MQ=255)
                                               cAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGa                                                                                                    <  2:1367342/150‑1 (MQ=255)
                                               cAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGa                                                                                                    <  1:826016/150‑1 (MQ=255)
                                               cAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGAc                                                                                                   >  2:515644/1‑151 (MQ=255)
                                                 aTTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGc                                                                                                 >  1:1715046/1‑151 (MQ=255)
                                                 aTTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATACAAAGGGGTACTTACGCGGCAGGTTGACGcc                                                                                                >  1:67572/1‑151 (MQ=255)
                                                     ccGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCata                                                                                             <  1:892313/151‑1 (MQ=255)
                                                         tGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATAcc                                                                                         >  2:96890/1‑151 (MQ=255)
                                                          gACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCg                                                                                        >  2:426536/1‑151 (MQ=255)
                                                             gggATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTg                                                                                     <  1:1207514/151‑1 (MQ=255)
                                                                aTGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGtt                                                                                  <  1:799343/151‑1 (MQ=255)
                                                                  ggTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTc                                                                                 <  1:288457/150‑1 (MQ=255)
                                                                  ggTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCg                                                                                <  1:1165400/151‑1 (MQ=255)
                                                                   gTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCgg                                                                               <  1:995638/151‑1 (MQ=255)
                                                                     ttGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGt                                                                              <  2:1690187/150‑1 (MQ=255)
                                                                           gAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCa                                                                       <  2:389321/151‑1 (MQ=255)
                                                                            aaGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCaa                                                                      <  1:1169593/151‑1 (MQ=255)
                                                                            aaGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCaa                                                                      <  1:808406/151‑1 (MQ=255)
                                                                            aaGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCa                                                                       >  1:741785/1‑150 (MQ=255)
                                                                              ggTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAAc                                                                    <  2:804627/151‑1 (MQ=255)
                                                                              ggTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAAc                                                                    >  2:1238105/1‑151 (MQ=255)
                                                                               gTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAAc                                                                    >  2:1256385/1‑150 (MQ=255)
                                                                                  aaaaTACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGAc                                                                >  2:1012339/1‑151 (MQ=255)
                                                                                   aaaTACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACt                                                               >  1:1698446/1‑151 (MQ=255)
                                                                                    aaTACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTc                                                              <  1:874030/151‑1 (MQ=255)
                                                                                    aaTACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTc                                                              >  1:1329167/1‑151 (MQ=255)
                                                                                      tACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGt                                                            >  2:786032/1‑151 (MQ=255)
                                                                                       aCTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGt                                                            <  2:881884/150‑1 (MQ=255)
                                                                                         ttGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCa                                                          >  1:740774/1‑150 (MQ=255)
                                                                                         ttGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCa                                                          >  1:547008/1‑150 (MQ=255)
                                                                                               aaCCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAAcgccgc                                                   >  2:1477983/1‑151 (MQ=255)
                                                                                                aCCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCt                                                  >  1:1361039/1‑151 (MQ=255)
                                                                                                aCCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCt                                                  >  1:204213/1‑151 (MQ=255)
                                                                                                 ccAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTg                                                 >  2:230965/1‑151 (MQ=255)
                                                                                                 ccAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTg                                                 >  1:887021/1‑151 (MQ=255)
                                                                                                  cAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGa                                                <  1:131907/151‑1 (MQ=255)
                                                                                                     aGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATcc                                             <  1:478430/151‑1 (MQ=255)
                                                                                                      ggAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATccc                                            >  1:1704193/1‑151 (MQ=255)
                                                                                                       gAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATccc                                            <  2:1661109/150‑1 (MQ=255)
                                                                                                       gAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCg                                           <  2:229124/151‑1 (MQ=255)
                                                                                                       gAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCg                                           <  1:1651894/151‑1 (MQ=255)
                                                                                                              cTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCcaccac                                    >  2:608563/1‑151 (MQ=255)
                                                                                                                ttCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACtt                                  >  2:119423/1‑151 (MQ=255)
                                                                                                                    tttGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACtttttt                              >  1:38157/1‑151 (MQ=255)
                                                                                                                     ttGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACtttttt                              <  2:1320048/150‑1 (MQ=255)
                                                                                                                     ttGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTc                             >  2:1379798/1‑151 (MQ=255)
                                                                                                                     ttGCGCGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCCCTGTTTCGGTGATCCCAAACAGACTCGACAACGCCGCTGATCCCGCCACCACtttttt                              <  1:572929/150‑1 (MQ=255)
                                                                                                                       gcgcGTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCt                            <  1:337997/150‑1 (MQ=255)
                                                                                                                        cgcgTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTgc                          >  1:698365/1‑151 (MQ=255)
                                                                                                                           gTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCa                       <  2:13742/151‑1 (MQ=255)
                                                                                                                           gTAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCa                       <  2:1114154/151‑1 (MQ=255)
                                                                                                                            tAGCCAATAATGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCAt                      >  2:305268/1‑151 (MQ=255)
                                                                                                                                 aataatGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGt                 >  2:781211/1‑151 (MQ=255)
                                                                                                                                  ataatGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATCCCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGtt                <  1:1102658/151‑1 (MQ=255)
                                                                                                                                    aatGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCg              <  2:1658664/151‑1 (MQ=255)
                                                                                                                                    aatGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCg              >  2:163619/1‑151 (MQ=255)
                                                                                                                                    aatGGTGGCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCg              <  2:917098/151‑1 (MQ=255)
                                                                                                                                       ggtggCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCag           <  1:1352839/151‑1 (MQ=255)
                                                                                                                                       ggtggCCCCCAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCa            <  2:780420/150‑1 (MQ=255)
                                                                                                                                                cAAGGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACg  <  1:1268350/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACCGATGGCAATGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACG  >  NC_000913/3904278‑3904572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: