Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 566,457 A→G pseudogene (82/312 nt) peaD → pseudogene; DLP12 prophage; replication protein family;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913566,4570AG97.3% 396.6 / 0.8 113pseudogene (82/312 nt)peaDpseudogene; DLP12 prophage; replication protein family;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base G (54/55);  total (57/56)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01

Only 100 of 113 total aligned reads displayed.

TGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGCG  >  NC_000913/566309‑566604
                                                                                                                                                     |                                                                                                                                                   
tGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGcc                                                                                                                                                   >  2:175393/1‑151 (MQ=255)
   gCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCaa                                                                                                                                                 >  1:756347/1‑150 (MQ=255)
   gCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAAc                                                                                                                                                <  2:341130/151‑1 (MQ=255)
    ccATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACt                                                                                                                                               >  1:216338/1‑151 (MQ=255)
         tAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTAc                                                                                                                                          >  1:388676/1‑151 (MQ=255)
          aaGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTAcc                                                                                                                                         <  2:370020/151‑1 (MQ=255)
           aGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTAcc                                                                                                                                         >  1:861103/1‑150 (MQ=255)
            gCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTAcc                                                                                                                                         >  2:1607865/1‑149 (MQ=255)
            gCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAg                                                                                                                                       <  1:1060065/151‑1 (MQ=255)
             ccGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGa                                                                                                                                      <  2:1701622/151‑1 (MQ=255)
              cGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATTAAATCGCGGGCGGTACGAGTATTGCCGGAAGCGCGGCCTGTATCCGGATGTCAGGACTTATCCGTGGAAATCGCACGCACATTACTGGTTGGTTGCCAACTTGTACCAGa                                                                                                                                      >  1:750365/1‑150 (MQ=255)
               gATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAAc                                                                                                                                    >  1:1648473/1‑151 (MQ=255)
                aTTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAAc                                                                                                                                    >  2:64558/1‑150 (MQ=255)
                       tGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGc                                                                                                                            >  1:16531/1‑151 (MQ=255)
                         ccTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCa                                                                                                                          <  2:1150482/151‑1 (MQ=255)
                            cGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCaa                                                                                                                         <  1:595065/149‑1 (MQ=255)
                            cGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATg                                                                                                                       <  1:396630/151‑1 (MQ=255)
                            cGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGAACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATg                                                                                                                       >  2:1537412/1‑151 (MQ=255)
                             gAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATgc                                                                                                                      <  2:580618/151‑1 (MQ=255)
                             gAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATgc                                                                                                                      >  1:242806/1‑151 (MQ=255)
                                accaccGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCgct                                                                                                                   <  2:31463/151‑1 (MQ=255)
                                accaccGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCgct                                                                                                                   <  1:818999/151‑1 (MQ=255)
                                accaccGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCgct                                                                                                                   <  2:1101894/151‑1 (MQ=255)
                                     cGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCgctggctg                                                                                                              <  1:1187034/151‑1 (MQ=255)
                                     cGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCgctggctg                                                                                                              <  1:707225/151‑1 (MQ=255)
                                     cGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCgctggctg                                                                                                              >  1:773083/1‑151 (MQ=255)
                                     cGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCgctggctg                                                                                                              >  2:27684/1‑151 (MQ=255)
                                      gAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCgctggctg                                                                                                              >  2:1640354/1‑150 (MQ=255)
                                      gAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCgctggctg                                                                                                              >  1:1181841/1‑150 (MQ=255)
                                          cGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAcgcg                                                                                                         >  1:1321580/1‑151 (MQ=255)
                                          cGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAcgcg                                                                                                         <  2:1622446/151‑1 (MQ=255)
                                          cGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAcgcg                                                                                                         >  1:859817/1‑151 (MQ=255)
                                           ggAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCgg                                                                                                        >  2:177165/1‑151 (MQ=255)
                                           ggAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCgg                                                                                                        >  1:811125/1‑151 (MQ=255)
                                              aTTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAAt                                                                                                     <  2:743367/151‑1 (MQ=255)
                                                ttCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAAtt                                                                                                    <  2:1118070/150‑1 (MQ=255)
                                                ttCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAAtt                                                                                                    <  1:236826/150‑1 (MQ=255)
                                                 tCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTCGCTGACGCGGAATTa                                                                                                   <  1:16161/150‑1 (MQ=255)
                                                  cATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACg                                                                                                 <  1:1683338/151‑1 (MQ=255)
                                                    tGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGgc                                                                                               >  2:932084/1‑151 (MQ=255)
                                                      aaaTCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGgcgc                                                                                             >  1:78799/1‑151 (MQ=255)
                                                      aaaTCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGgcgc                                                                                             <  2:1190104/151‑1 (MQ=255)
                                                       aaTCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGgcgc                                                                                             >  1:204743/1‑150 (MQ=255)
                                                       aaTCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCa                                                                                            <  2:410564/151‑1 (MQ=255)
                                                         tCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCa                                                                                            >  1:1137566/1‑149 (MQ=255)
                                                         acccGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCa                                                                                            >  1:277242/2‑149 (MQ=255)
                                                          cccGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAgg                                                                                         >  1:499977/1‑151 (MQ=255)
                                                            cGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCt                                                                                       >  1:374020/1‑151 (MQ=255)
                                                             gggAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTg                                                                                      >  2:908940/1‑151 (MQ=255)
                                                                aGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCg                                                                                   <  1:207097/151‑1 (MQ=255)
                                                                  gTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGATGCGGAATTACGGCGCAAGGCTGCCGAt                                                                                 <  2:265600/151‑1 (MQ=255)
                                                                  gTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGAt                                                                                 >  1:308647/1‑151 (MQ=255)
                                                                   tACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATg                                                                                >  1:821215/1‑151 (MQ=255)
                                                                   tACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATg                                                                                >  1:1037302/1‑151 (MQ=255)
                                                                   tACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATg                                                                                >  1:265746/1‑151 (MQ=255)
                                                                   caCGAGTATTGCCGCAAGCGTCACCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCCACTTGCACCAGAACATGCGGACCAATGCGCTGGCTGACGCCGATTTACGGCCCAAGGATGCCGATg                                                                                >  1:119422/2‑151 (MQ=255)
                                                                    aCGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATg                                                                                >  2:547291/1‑150 (MQ=255)
                                                                    aCGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGa                                                                               <  1:877783/151‑1 (MQ=255)
                                                                     cGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTGCCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGa                                                                               >  1:494701/1‑150 (MQ=255)
                                                                         tATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTg                                                                           >  2:756990/1‑150 (MQ=255)
                                                                         tATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTg                                                                           >  1:469830/1‑150 (MQ=255)
                                                                             gCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTg                                                                      <  2:169394/151‑1 (MQ=255)
                                                                              ccGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGt                                                                     >  2:1711782/1‑151 (MQ=255)
                                                                                 gAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATg                                                                  <  2:1199497/151‑1 (MQ=255)
                                                                                  aaGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATg                                                                  >  2:159157/1‑150 (MQ=255)
                                                                                     cGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAg                                                              <  1:629536/151‑1 (MQ=255)
                                                                                     cGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAg                                                              <  1:486771/151‑1 (MQ=255)
                                                                                      gTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAgc                                                             >  2:1551056/1‑151 (MQ=255)
                                                                                       tGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAgcg                                                            >  1:39335/1‑151 (MQ=255)
                                                                                           cTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGa                                                         >  2:1281878/1‑150 (MQ=255)
                                                                                             gTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAAtt                                                      <  1:993761/151‑1 (MQ=255)
                                                                                             gTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAAtt                                                      >  2:211911/1‑151 (MQ=255)
                                                                                             gTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAAt                                                       <  1:888895/150‑1 (MQ=255)
                                                                                              tATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAAtt                                                      >  1:1625248/1‑150 (MQ=255)
                                                                                              tATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAAtt                                                      >  2:1679624/1‑150 (MQ=255)
                                                                                               aTCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTa                                                     >  1:527054/1‑150 (MQ=255)
                                                                                                 ccGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAAcc                                                  >  1:1665902/1‑151 (MQ=255)
                                                                                                   ggATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTCCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCgt                                                <  2:337023/151‑1 (MQ=255)
                                                                                                       gCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCgtggtg                                            <  2:811191/151‑1 (MQ=255)
                                                                                                         cAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCgtggtg                                            >  2:1326595/1‑150 (MQ=255)
                                                                                                          agagTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCgtggtg                                            >  1:82252/1‑149 (MQ=255)
                                                                                                          agagTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTgag                                          <  1:175382/151‑1 (MQ=255)
                                                                                                             gTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACg                                       >  2:695096/1‑151 (MQ=255)
                                                                                                             gTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACg                                       <  2:867406/151‑1 (MQ=255)
                                                                                                              tCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACg                                       >  1:1192089/1‑150 (MQ=255)
                                                                                                                 tATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATa                                    <  1:864798/150‑1 (MQ=255)
                                                                                                                   tCCGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCt                                 >  2:1091387/1‑151 (MQ=255)
                                                                                                                    ccGTGGAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTg                                <  2:714476/151‑1 (MQ=255)
                                                                                                                        ggAAATCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAAc                             <  2:1111002/150‑1 (MQ=255)
                                                                                                                          aaaTCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAg                          <  2:1666013/151‑1 (MQ=255)
                                                                                                                          aaaTCGAACGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAg                          <  2:827704/151‑1 (MQ=255)
                                                                                                                                aaCGCGCATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAaac                    <  2:821281/151‑1 (MQ=255)
                                                                                                                                     gcATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACtt               <  1:1607767/151‑1 (MQ=255)
                                                                                                                                     gcATTACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACt                <  1:1543802/150‑1 (MQ=255)
                                                                                                                                        ttACTGGTTGGTTGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCt            >  2:56687/1‑151 (MQ=255)
                                                                                                                                             ggttggttGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGtt         <  2:773147/149‑1 (MQ=255)
                                                                                                                                             ggttggttGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTa        <  1:908872/150‑1 (MQ=255)
                                                                                                                                                 ggttGCCAACTTGTACCAGAACATGCTGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGc   <  1:1228726/151‑1 (MQ=255)
                                                                                                                                                 ggttGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGc   <  1:332470/151‑1 (MQ=255)
                                                                                                                                                  gttGCCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGCg  <  1:889377/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGCG  >  NC_000913/566309‑566604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: