Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,003,303 A→G R292R (CGT→CGC)  rarD ← putative chloramphenical resistance permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,003,3030AG99.0% 370.1 / ‑4.7 100R292R (CGT→CGC) rarDputative chloramphenical resistance permease
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (57/42);  total (57/43)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 102 total aligned reads displayed.

AATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAA  >  NC_000913/4003155‑4003448
                                                                                                                                                    |                                                                                                                                                 
 aTCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTgcgc                                                                                                                                                >  2:840751/1‑151 (MQ=255)
  tCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCt                                                                                                                                               >  2:1176580/1‑151 (MQ=255)
  tCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCt                                                                                                                                               >  1:1708150/1‑151 (MQ=255)
     tACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCt                                                                                                                                               <  1:1651856/148‑1 (MQ=255)
      aCCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTctgcgctgcg                                                                                                                                            <  1:1034559/150‑1 (MQ=255)
      aCCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGt                                                                                                                                           <  2:1658062/151‑1 (MQ=255)
       ccGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGt                                                                                                                                           >  1:424999/1‑150 (MQ=255)
        cGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGtat                                                                                                                                         <  2:983704/151‑1 (MQ=255)
         gTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGtata                                                                                                                                        <  2:1266227/151‑1 (MQ=255)
         gTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGtata                                                                                                                                        <  1:434967/151‑1 (MQ=255)
               tGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGTTGCGTATAAATCGc                                                                                                                                  >  2:577473/1‑151 (MQ=255)
               tGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGc                                                                                                                                  >  1:1148812/1‑151 (MQ=255)
                 ttGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcat                                                                                                                                >  1:541525/1‑151 (MQ=255)
                     gATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcatccat                                                                                                                            <  1:840688/151‑1 (MQ=255)
                       tAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcatccatc                                                                                                                           >  2:948022/1‑150 (MQ=255)
                         aaTATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATcaca                                                                                                                        <  1:253429/151‑1 (MQ=255)
                                gTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTg                                                                                                                 >  2:1667986/1‑151 (MQ=255)
                                 tAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGc                                                                                                                >  1:1643572/1‑151 (MQ=255)
                                  aaCATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGcc                                                                                                               >  1:453006/1‑151 (MQ=255)
                                   aCATAAATTTTACATCCTTGTATGAGTCACCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCa                                                                                                              <  1:1625789/151‑1 (MQ=255)
                                     aTAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAgc                                                                                                            <  2:267239/151‑1 (MQ=255)
                                     aTAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAgc                                                                                                            <  1:1526965/151‑1 (MQ=255)
                                        aaTTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCGATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGcc                                                                                                         >  2:1313090/1‑151 (MQ=255)
                                            ttACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCAcc                                                                                                      <  1:389261/150‑1 (MQ=255)
                                             tACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCAccc                                                                                                     >  1:664960/1‑150 (MQ=255)
                                             tACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCAccc                                                                                                     >  2:654693/1‑150 (MQ=255)
                                              aCATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCaa                                                                                                   <  1:696005/151‑1 (MQ=255)
                                              aCATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCaa                                                                                                   <  2:1384417/151‑1 (MQ=255)
                                              aCATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCa                                                                                                    <  1:58655/150‑1 (MQ=255)
                                                aTCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAAt                                                                                                 >  1:1074870/1‑151 (MQ=255)
                                                    ttGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATaa                                                                                               >  1:478774/1‑149 (MQ=255)
                                                        aTGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGa                                                                                         <  1:636228/151‑1 (MQ=255)
                                                          gAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGaaa                                                                                       <  1:222681/151‑1 (MQ=255)
                                                          gAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGaaa                                                                                       <  2:665019/151‑1 (MQ=255)
                                                          gAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGaa                                                                                        <  2:1611497/150‑1 (MQ=255)
                                                              ctcCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTc                                                                                    >  1:1644213/1‑150 (MQ=255)
                                                                 cGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCAcc                                                                                 >  2:814363/1‑150 (MQ=255)
                                                                  agTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCAt                                                                               >  2:1663000/2‑151 (MQ=255)
                                                                    tCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCt                                                                             >  2:1056171/1‑151 (MQ=255)
                                                                     cAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCtt                                                                            <  2:1430215/151‑1 (MQ=255)
                                                                     cAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCt                                                                             <  1:353283/150‑1 (MQ=255)
                                                                       gCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTAt                                                                          <  1:480400/151‑1 (MQ=255)
                                                                        cATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATc                                                                         <  2:507220/151‑1 (MQ=255)
                                                                         aTGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCg                                                                        >  2:1496891/1‑151 (MQ=255)
                                                                           ggCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCgg                                                                       <  2:1624511/150‑1 (MQ=255)
                                                                           ggCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGc                                                                      <  2:948213/151‑1 (MQ=255)
                                                                            gCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCa                                                                     <  2:1509873/151‑1 (MQ=255)
                                                                             cAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCAc                                                                    >  1:251573/1‑151 (MQ=255)
                                                                               atatGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAGGTCACCATCTTATCGGCAccc                                                                  >  2:1681861/1‑151 (MQ=255)
                                                                                tatGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCg                                                                 >  2:1321807/1‑151 (MQ=255)
                                                                                 atGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCgg                                                                <  1:1440550/151‑1 (MQ=255)
                                                                                   gCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGt                                                               <  1:1105994/150‑1 (MQ=255)
                                                                                       aCTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTc                                                          >  1:615692/1‑151 (MQ=255)
                                                                                            cATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCAt                                                     <  2:1650125/151‑1 (MQ=255)
                                                                                             aTGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCAt                                                     >  1:1235389/1‑150 (MQ=255)
                                                                                              tGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATa                                                    >  2:478910/1‑150 (MQ=255)
                                                                                                   gagTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGt                                              <  1:1496791/151‑1 (MQ=255)
                                                                                                     gTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTca                                            <  1:1321712/151‑1 (MQ=255)
                                                                                                      tCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTcac                                           >  1:1198983/1‑151 (MQ=255)
                                                                                                       cGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTcaca                                          <  1:655912/151‑1 (MQ=255)
                                                                                                         gCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCGCCATAAAACGTCAcagc                                        <  1:1398159/151‑1 (MQ=255)
                                                                                                           atatTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagcca                                      <  2:872443/151‑1 (MQ=255)
                                                                                                             atTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccagc                                    >  2:721637/1‑151 (MQ=255)
                                                                                                              ttttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccagc                                    >  2:891857/1‑150 (MQ=255)
                                                                                                               tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccagc                                    >  2:21288/1‑149 (MQ=255)
                                                                                                               tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccag                                     >  1:1530148/1‑148 (MQ=255)
                                                                                                               tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCcagca                                   <  2:444379/150‑1 (MQ=255)
                                                                                                               tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCcagca                                   <  2:1510817/150‑1 (MQ=255)
                                                                                                                ttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAgg                                 >  2:1662227/1‑151 (MQ=255)
                                                                                                                ttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAgg                                 >  1:1357687/1‑151 (MQ=255)
                                                                                                                 tttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAgg                                 >  2:1404310/1‑150 (MQ=255)
                                                                                                                 tttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAgg                                 >  1:801433/1‑150 (MQ=255)
                                                                                                                 tttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAgg                                 >  1:383059/1‑150 (MQ=255)
                                                                                                                 tttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAgg                                 >  1:181878/1‑150 (MQ=255)
                                                                                                                 tttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAgg                                 >  1:1598675/1‑150 (MQ=255)
                                                                                                                   tCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAAc                              >  2:723883/1‑151 (MQ=255)
                                                                                                                   tCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAAc                              >  2:1378658/1‑151 (MQ=255)
                                                                                                                   tCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAAc                              >  2:1141430/1‑151 (MQ=255)
                                                                                                                    cAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAAc                              >  1:886631/1‑150 (MQ=255)
                                                                                                                    cAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAAc                              <  2:656343/150‑1 (MQ=255)
                                                                                                                       aaTATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATc                           >  2:328658/1‑150 (MQ=255)
                                                                                                                         tatatTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCa                          >  2:659015/1‑149 (MQ=255)
                                                                                                                         tatatTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGc                        >  2:195729/1‑151 (MQ=255)
                                                                                                                           tatttattTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGt                      >  1:1395447/1‑151 (MQ=255)
                                                                                                                            atttatttTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGt                      <  1:1378564/150‑1 (MQ=255)
                                                                                                                            atttatttTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTc                     <  2:1328990/151‑1 (MQ=255)
                                                                                                                               tattttttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCggg                  >  2:1057656/1‑151 (MQ=255)
                                                                                                                               tattttttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCgg                   <  2:615877/150‑1 (MQ=255)
                                                                                                                                   ttttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCa               >  2:235849/1‑150 (MQ=255)
                                                                                                                                      tatttGGACGTTCTGCGCTGCGTATAAATCTCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGACACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGCACATCAGCGTCGGGCCAATGt           >  2:1629850/1‑151 (MQ=255)
                                                                                                                                      tatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGt           >  2:1381532/1‑151 (MQ=255)
                                                                                                                                      tatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGt           >  2:829214/1‑151 (MQ=255)
                                                                                                                                      tatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGt           >  1:143453/1‑151 (MQ=255)
                                                                                                                                       atttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGt           <  2:425033/150‑1 (MQ=255)
                                                                                                                                        tttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTAc         >  1:344564/1‑151 (MQ=255)
                                                                                                                                         ttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTAc         <  2:1333941/150‑1 (MQ=255)
                                                                                                                                         ttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTAc         <  1:1050626/150‑1 (MQ=255)
                                                                                                                                         ttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACt        >  2:973720/1‑151 (MQ=255)
                                                                                                                                              cGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGaa    >  2:727327/1‑150 (MQ=255)
                                                                                                                                                 tCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGaaaa  >  2:786721/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAA  >  NC_000913/4003155‑4003448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑