Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,279,290 A→G D271G (GAT→GGT)  ghxP → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,2900AG96.3% 299.3 / 1.4 82D271G (GAT→GGT) ghxPguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (43/36);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGC  >  NC_000913/4279142‑4279431
                                                                                                                                                    |                                                                                                                                             
ccTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTg                                                                                                                                             >  1:1560711/1‑151 (MQ=255)
       tCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCgg                                                                                                                                      >  2:1080408/1‑151 (MQ=255)
                ccGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCg                                                                                                                             >  2:665699/1‑151 (MQ=255)
                  ggCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTg                                                                                                                           >  2:722013/1‑151 (MQ=255)
                   gCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGc                                                                                                                          <  2:1082202/151‑1 (MQ=255)
                      ttttCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGt                                                                                                                       >  2:16780/1‑151 (MQ=255)
                       tttCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGt                                                                                                                       <  2:1493053/150‑1 (MQ=255)
                       tttCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTc                                                                                                                      >  2:536857/1‑151 (MQ=255)
                        ttCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGTTTGGCAGCCTGGACATTATGGGCGCGCTGAAGCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGt                                                                                                                       <  1:982863/149‑1 (MQ=255)
                        ttCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTc                                                                                                                      <  2:445620/150‑1 (MQ=255)
                        ttCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCg                                                                                                                     >  1:775985/1‑151 (MQ=255)
                           gCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCg                                                                                                                  <  1:337039/151‑1 (MQ=255)
                                gCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGcc                                                                                                               >  1:832894/1‑149 (MQ=255)
                                gCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGcc                                                                                                               >  1:488710/1‑149 (MQ=255)
                                gCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCa                                                                                                              <  2:407141/150‑1 (MQ=255)
                                   ttCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCg                                                                                                          >  1:619197/1‑151 (MQ=255)
                                     cATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGa                                                                                                         <  1:1712186/150‑1 (MQ=255)
                                      aTTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAAc                                                                                                       >  2:834572/1‑151 (MQ=255)
                                        tGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAAcc                                                                                                      <  2:915403/150‑1 (MQ=255)
                                         gAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACct                                                                                                     <  1:198260/150‑1 (MQ=255)
                                         gAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACct                                                                                                     <  1:112900/150‑1 (MQ=255)
                                         gAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACct                                                                                                     <  1:224643/150‑1 (MQ=255)
                                          aGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACctg                                                                                                    >  2:1230336/1‑150 (MQ=255)
                                           gCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACctgct                                                                                                  >  2:108896/1‑151 (MQ=255)
                                             gATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTgg                                                                                                <  1:1037733/151‑1 (MQ=255)
                                                gAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATa                                                                                             <  1:535397/151‑1 (MQ=255)
                                                  aaaCGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATaa                                                                                            >  1:1279852/1‑150 (MQ=255)
                                                     cGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATaa                                                                                            >  1:1077560/1‑147 (MQ=255)
                                                      ggCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATg                                                                                       <  1:498004/151‑1 (MQ=255)
                                                       gCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATgg                                                                                      <  2:457683/151‑1 (MQ=255)
                                                       gCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATgg                                                                                      >  2:1593680/1‑151 (MQ=255)
                                                        cAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATggg                                                                                     <  1:802598/151‑1 (MQ=255)
                                                         aaTTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGc                                                                                    <  1:1382508/151‑1 (MQ=255)
                                                         aaTTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGc                                                                                    <  1:1544695/151‑1 (MQ=255)
                                                               cTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGatc                                                                               >  1:1244277/1‑150 (MQ=255)
                                                                tGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGatcat                                                                             >  1:1051679/1‑151 (MQ=255)
                                                                tGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGatcat                                                                             >  2:1706793/1‑151 (MQ=255)
                                                                 gATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGatcatc                                                                            <  1:43459/151‑1 (MQ=255)
                                                                   ttGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCg                                                                           >  1:582465/1‑150 (MQ=255)
                                                                     ggCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGAc                                                                         <  1:1357207/150‑1 (MQ=255)
                                                                      gCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACgg                                                                       >  1:1536702/1‑151 (MQ=255)
                                                                        aGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTg                                                                     >  2:986640/1‑151 (MQ=255)
                                                                            tGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGa                                                                  >  1:81369/1‑150 (MQ=255)
                                                                                cATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGc                                                              >  1:1402508/1‑150 (MQ=255)
                                                                                   tATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcact                                                           <  1:923992/150‑1 (MQ=255)
                                                                                    aTGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactg                                                          >  1:1473234/1‑150 (MQ=255)
                                                                                      gggCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgacc                                                       >  1:1153033/1‑151 (MQ=255)
                                                                                      gggCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgacc                                                       >  2:246756/1‑151 (MQ=255)
                                                                                       ggCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGGGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgacca                                                      <  1:1596194/151‑1 (MQ=255)
                                                                                        gcgcgcTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccac                                                     <  2:1273347/151‑1 (MQ=255)
                                                                                           cgcTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactga                                                  <  2:269175/151‑1 (MQ=255)
                                                                                                 aTCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCAt                                            <  2:774659/151‑1 (MQ=255)
                                                                                                 aTCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCAt                                            <  2:690903/151‑1 (MQ=255)
                                                                                                      gTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagc                                        >  1:924333/1‑150 (MQ=255)
                                                                                                      gTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagc                                        >  2:418681/1‑150 (MQ=255)
                                                                                                         gTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCg                                    <  1:665640/151‑1 (MQ=255)
                                                                                                              gCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGttt                                 <  2:942956/149‑1 (MQ=255)
                                                                                                              gCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGttt                                 <  1:1283115/149‑1 (MQ=255)
                                                                                                              gCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTtc                               >  2:23959/1‑151 (MQ=255)
                                                                                                                 aaGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCTTCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTctct                            >  2:1644708/1‑151 (MQ=255)
                                                                                                                  aGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTg                           >  1:819449/1‑151 (MQ=255)
                                                                                                                  aGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTg                           >  2:1197095/1‑151 (MQ=255)
                                                                                                                   gCGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTgg                          <  2:1335117/151‑1 (MQ=255)
                                                                                                                    cGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                         <  1:16778/151‑1 (MQ=255)
                                                                                                                    cGTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                         >  1:352844/1‑151 (MQ=255)
                                                                                                                    agTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                         >  2:1666760/2‑151 (MQ=255)
                                                                                                                     gTTCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                         <  2:832956/150‑1 (MQ=255)
                                                                                                                       tCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtg                      >  2:454742/1‑151 (MQ=255)
                                                                                                                       tCTGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCt                       <  1:721955/150‑1 (MQ=255)
                                                                                                                         tGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggt                    >  1:753525/1‑151 (MQ=255)
                                                                                                                         tGGCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtgg                     >  1:426453/1‑150 (MQ=255)
                                                                                                                          ggCGCTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtgg                     <  2:1564892/149‑1 (MQ=255)
                                                                                                                            cgcTGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtggg                 <  2:1534149/151‑1 (MQ=255)
                                                                                                                               tGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggtg               >  2:1267124/1‑150 (MQ=255)
                                                                                                                               tGGTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggtg               >  1:22428/1‑150 (MQ=255)
                                                                                                                                 gTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGc              <  2:488758/149‑1 (MQ=255)
                                                                                                                                 gTGATGACGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCgg            <  2:69819/151‑1 (MQ=255)
                                                                                                                                    atgaCGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTc         >  1:1900/1‑151 (MQ=255)
                                                                                                                                       aCGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCgg      <  2:687961/151‑1 (MQ=255)
                                                                                                                                        cGGCAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCgg      >  2:319280/1‑150 (MQ=255)
                                                                                                                                           cAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGc  <  1:972687/151‑1 (MQ=255)
                                                                                                                                           cAGTATTTGGTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGc  >  1:1132247/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGC  >  NC_000913/4279142‑4279431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: