Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,281,308 A→G A443A (GCA→GCG yjcE → putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,281,3080AG99.1% 393.1 / ‑4.5 106A443A (GCA→GCGyjcEputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (62/43);  total (62/44)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 107 total aligned reads displayed.

ATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGA  >  NC_000913/4281163‑4281454
                                                                                                                                                 |                                                                                                                                                  
  gAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTg                                                                                                                                              <  1:206550/150‑1 (MQ=255)
   aGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTgg                                                                                                                                             >  2:636674/1‑150 (MQ=255)
     cTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCg                                                                                                                                           >  2:1519070/1‑150 (MQ=255)
     cTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCg                                                                                                                                           >  2:746130/1‑150 (MQ=255)
        gtgtTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATtg                                                                                                                                       <  2:1009160/151‑1 (MQ=255)
              cTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGAGGCGATTGTGGCg                                                                                                                                  >  1:1199145/1‑150 (MQ=255)
               aggcggcTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGAt                                                                                                                                >  2:796779/2‑151 (MQ=255)
                ggcggcTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCggt                                                                                                                                >  1:944671/1‑148 (MQ=255)
                ggcggcTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGAt                                                                                                                                <  1:862191/150‑1 (MQ=255)
                 gcggtTGGAGTCATTCTCTTCTCGCTGTTTGTCGGAGTGGTGATGTTGCCTATTCTGCTACAACTCATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATcc                                                                                                                              <  2:1393380/151‑1 (MQ=255)
                   ggcTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAg                                                                                                                            <  2:683414/151‑1 (MQ=255)
                   ggcTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAg                                                                                                                            <  2:1449289/151‑1 (MQ=255)
                    gcTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATcc                                                                                                                              >  2:349212/1‑148 (MQ=255)
                     cTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGa                                                                                                                           >  2:1563467/1‑150 (MQ=255)
                       ggCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGaaa                                                                                                                         >  2:268415/1‑150 (MQ=255)
                               tctcttctcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACTCATTGAAGTCGCGGATCATTCGCAACAATTGAATGAGGATCGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATggagga                                                                                                                 <  2:844710/150‑1 (MQ=255)
                               tctcttctcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATggagga                                                                                                                 <  2:1681144/150‑1 (MQ=255)
                                  cttctcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGc                                                                                                               <  1:1280552/149‑1 (MQ=255)
                                   ttctcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGt                                                                                                             >  1:19028/1‑150 (MQ=255)
                                   ttctcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGACCCAGGAAATGGAGGGGCggc                                                                                                            >  1:712814/1‑149 (MQ=255)
                                    tctcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTc                                                                                                            <  2:806179/150‑1 (MQ=255)
                                    tctcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTc                                                                                                            >  1:407663/1‑150 (MQ=255)
                                    tctcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCt                                                                                                           >  2:868755/1‑151 (MQ=255)
                                     ctcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTg                                                                                                          >  1:1364824/1‑151 (MQ=255)
                                       cGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTggc                                                                                                        <  1:370342/151‑1 (MQ=255)
                                       cGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTgg                                                                                                         >  2:417034/1‑150 (MQ=255)
                                       cGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTgg                                                                                                         <  2:1411733/150‑1 (MQ=255)
                                       cGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTgg                                                                                                         <  2:469410/150‑1 (MQ=255)
                                         cTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTggcgg                                                                                                      >  2:948039/1‑151 (MQ=255)
                                         cTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTggcg                                                                                                       <  2:1250956/150‑1 (MQ=255)
                                            tttGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGCAAGAGGAAAGCATTGCGCGAGCGGCAACGGCGGAAGAGGTGATTGTGGCGATGCAGAAAATGGAGGAGGGGCTGGcggcgg                                                                                                   >  1:415679/1‑151 (MQ=255)
                                               gTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGAt                                                                                                 <  1:324993/150‑1 (MQ=255)
                                               gTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGAt                                                                                                 <  1:664718/150‑1 (MQ=255)
                                                 cGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATAcc                                                                                              >  2:420357/1‑151 (MQ=255)
                                                  ggCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATAcc                                                                                              >  2:1082981/1‑150 (MQ=255)
                                                  ggCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCg                                                                                             >  1:703255/1‑151 (MQ=255)
                                                       ggtgATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAaga                                                                                         <  1:1113267/150‑1 (MQ=255)
                                                       ggtgATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAaga                                                                                         <  1:1524730/150‑1 (MQ=255)
                                                         tgATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAgaga                                                                                       >  2:1382164/1‑150 (MQ=255)
                                                         tgATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAgaga                                                                                       >  2:1576846/1‑150 (MQ=255)
                                                          gATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGaa                                                                                      >  1:1533925/1‑150 (MQ=255)
                                                                 ccTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGAt                                                                               >  1:162091/1‑150 (MQ=255)
                                                                  cTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATa                                                                              >  1:1414614/1‑150 (MQ=255)
                                                                    aTTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATaa                                                                             <  2:1549580/149‑1 (MQ=255)
                                                                       cTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgc                                                                        >  1:1236741/1‑151 (MQ=255)
                                                                         gCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgc                                                                        <  2:577268/149‑1 (MQ=255)
                                                                         gCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATCGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgc                                                                        <  1:535981/149‑1 (MQ=255)
                                                                          cTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgctg                                                                      >  1:953040/1‑150 (MQ=255)
                                                                            acaacaCATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCtt                                                                   >  1:1685576/1‑151 (MQ=255)
                                                                               acacATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACg                                                                <  1:190228/151‑1 (MQ=255)
                                                                                 acaTTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACgg                                                               >  2:805284/1‑150 (MQ=255)
                                                                                 acaTTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACgg                                                               >  2:450391/1‑150 (MQ=255)
                                                                                    ttGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAgg                                                            >  2:48633/1‑150 (MQ=255)
                                                                                      gAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCa                                                         <  1:853261/151‑1 (MQ=255)
                                                                                           cgcgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTtct                                                    <  1:285258/151‑1 (MQ=255)
                                                                                           cgcgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTtct                                                    >  1:341612/1‑151 (MQ=255)
                                                                                            gcgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTtctc                                                   >  2:569293/1‑151 (MQ=255)
                                                                                            gcgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTtctc                                                   <  2:497480/151‑1 (MQ=255)
                                                                                            gcgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCCGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGGCAGTtct                                                    >  2:524212/1‑150 (MQ=255)
                                                                                             cgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTtctc                                                   <  2:318379/150‑1 (MQ=255)
                                                                                             cgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTcg                                                  <  2:515496/151‑1 (MQ=255)
                                                                                             cgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTcg                                                  >  1:1551627/1‑151 (MQ=255)
                                                                                             cgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTcg                                                  <  2:1309788/151‑1 (MQ=255)
                                                                                              ggATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTcgc                                                 <  2:772865/151‑1 (MQ=255)
                                                                                              ggATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTcgc                                                 >  2:189139/1‑151 (MQ=255)
                                                                                               gATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTcgcg                                                <  2:1051057/151‑1 (MQ=255)
                                                                                                aTCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTcgcg                                                >  2:1152448/1‑150 (MQ=255)
                                                                                                aTCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTcgcg                                                >  1:1646642/1‑150 (MQ=255)
                                                                                                         aacaaTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGCGGTCAGTTCTCGCGTCATTgg                                         >  1:1279055/1‑148 (MQ=255)
                                                                                                         aacaaTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTa                                       >  2:578316/1‑150 (MQ=255)
                                                                                                           caaTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAAcc                                    <  2:857620/151‑1 (MQ=255)
                                                                                                             aTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTg                                  <  1:931378/151‑1 (MQ=255)
                                                                                                              ttGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGc                                 >  2:151161/1‑151 (MQ=255)
                                                                                                               tGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGTGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCCGTTCTCGCGTCATTGGTAACCTGcg                                >  2:1354243/1‑151 (MQ=255)
                                                                                                               tGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGcg                                >  2:1051219/1‑151 (MQ=255)
                                                                                                                gAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGcg                                >  2:50013/1‑150 (MQ=255)
                                                                                                                gAAAGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGcg                                >  1:255773/1‑150 (MQ=255)
                                                                                                                 aaaGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGcgtc                              >  1:488277/1‑151 (MQ=255)
                                                                                                                  aaGAGGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGcgtc                              >  1:1005389/1‑150 (MQ=255)
                                                                                                                   agagGAACGTATTGCGCGAGCGGCAACAGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGcgtcgt                            >  2:1420989/1‑151 (MQ=255)
                                                                                                                    gagGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCgtcgtc                           <  1:1267428/151‑1 (MQ=255)
                                                                                                                     agGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGtcgtcg                          <  2:176959/151‑1 (MQ=255)
                                                                                                                     agGAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGtcgtcg                          <  1:1406624/151‑1 (MQ=255)
                                                                                                                     agGAACGTATTGCGCGAGCGGCAACGGCGGAAGCGGCGCTTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGAAACCGGAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCCGcgacgt                            >  2:709717/1‑149 (MQ=255)
                                                                                                                      ggAACGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTcgc                         >  2:587313/1‑151 (MQ=255)
                                                                                                                         aCGTATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGcc                      >  1:1016853/1‑151 (MQ=255)
                                                                                                                            tATTGCGCGAGCGGCAACGGCGGAAGGGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGAt                   >  1:579243/1‑151 (MQ=255)
                                                                                                                                gcgcGAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTATGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGa                >  2:95119/1‑150 (MQ=255)
                                                                                                                                  tcgAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAAACAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACg              >  1:736316/2‑150 (MQ=255)
                                                                                                                                  gcgAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGt             >  2:310200/1‑151 (MQ=255)
                                                                                                                                   cgAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACg              >  2:464966/1‑149 (MQ=255)
                                                                                                                                   cgAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTa            <  2:1236839/151‑1 (MQ=255)
                                                                                                                                   cgAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTa            <  1:349185/151‑1 (MQ=255)
                                                                                                                                    gAGCGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTaa           <  1:746069/151‑1 (MQ=255)
                                                                                                                                       cGGCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAacga        <  2:56852/151‑1 (MQ=255)
                                                                                                                                         gCAACGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAacgacg      <  2:706964/151‑1 (MQ=255)
                                                                                                                                           aaCGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGtt    <  2:661657/151‑1 (MQ=255)
                                                                                                                                           aaCGGCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGGCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGtt    >  1:1498710/1‑151 (MQ=255)
                                                                                                                                             cggcggAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTg   >  2:561673/1‑150 (MQ=255)
                                                                                                                                              ggCGGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGa  >  2:631665/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGA  >  NC_000913/4281163‑4281454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: