Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,366,223 A→G F184S (TTC→TCC)  dcuA ← C4‑dicarboxylate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,366,2230AG99.2% 417.4 / ‑5.3 121F184S (TTC→TCC) dcuAC4‑dicarboxylate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (74/45);  total (74/47)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 123 total aligned reads displayed.

AGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAT  >  NC_000913/4366079‑4366369
                                                                                                                                                |                                                                                                                                                  
agcagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGAc                                                                                                                                               >  1:1139005/1‑150 (MQ=255)
agcagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGAc                                                                                                                                               >  1:840838/1‑150 (MQ=255)
agcagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCACTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGAc                                                                                                                                               >  1:771502/1‑150 (MQ=255)
 gcagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACAt                                                                                                                                             <  1:286270/151‑1 (MQ=255)
      aaCAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCa                                                                                                                                        <  2:446769/151‑1 (MQ=255)
       aCAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAg                                                                                                                                       <  2:1076396/151‑1 (MQ=255)
            cAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAAcc                                                                                                                                   <  2:216835/150‑1 (MQ=255)
                     gTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAggg                                                                                                                         >  1:828194/1‑151 (MQ=255)
                      tttttGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGt                                                                                                                        >  1:743301/1‑151 (MQ=255)
                       ttttGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTg                                                                                                                       >  2:1519448/1‑151 (MQ=255)
                       ttttGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTg                                                                                                                       >  2:1509865/1‑151 (MQ=255)
                        tttGCACCGGATTTGATTTCAATCTGCTTTCCCCCGCGCAGTCCAACCAGGCCCTCTTCCAGACGCTTGCGAAAAATCGGATCGTCAGAGAGTTTGGAGTTAAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTg                                                                                                                       <  1:785442/150‑1 (MQ=255)
                             aCCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACggg                                                                                                                 >  2:560418/1‑151 (MQ=255)
                                ggATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGCCCAGGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGAt                                                                                                               <  2:293734/150‑1 (MQ=255)
                                ggATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATg                                                                                                              >  1:62956/1‑151 (MQ=255)
                                 gatttgatttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATg                                                                                                              >  1:1426935/1‑150 (MQ=255)
                                 gatttgatttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATg                                                                                                              >  2:1280175/1‑150 (MQ=255)
                                   tttgatttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGAcc                                                                                                           >  1:508558/1‑151 (MQ=255)
                                     tgatttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCAc                                                                                                         >  1:40410/1‑151 (MQ=255)
                                        tttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACgg                                                                                                       >  2:530268/1‑150 (MQ=255)
                                         ttCAATCCGCTGTACCCCGCAGAGTTGAACCAGGCCCTCTCCCTCACGCTGGAGATAATGCGAACCGTCAAAGGGTTGGGAGTGGATCAGCTTATACTCCTGGGGGGACTTCCCAAGAACCGCCAGCAGGGTGGACGGGATGACCACGga                                                                                                      <  1:1090841/150‑1 (MQ=255)
                                            aaTCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  1:1518826/1‑151 (MQ=255)
                                               cTGTTTTTCCCCGCGCAGTTCACCCAGGCCCTCTTCCAGACGCTGGCGATAAATCGGATCGTCAGAGAGTGTGGAGTCGAACAGTATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGgaga                                                                                                    <  1:1005069/146‑1 (MQ=255)
                                                tGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  1:148842/1‑147 (MQ=255)
                                                 gCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATg                                                                                             >  1:879377/1‑151 (MQ=255)
                                                  cTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATgg                                                                                            >  2:222544/1‑151 (MQ=255)
                                                  cTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATgg                                                                                            >  2:1198379/1‑151 (MQ=255)
                                                   ttttCCCCGCGCAGTTCACCCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTGAGAGAGTTTGGAGTTAACCAGCATGGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAg                                                                                                <  2:1441991/146‑1 (MQ=255)
                                                   ttttCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATgg                                                                                            >  1:1189843/1‑150 (MQ=255)
                                                   ttttCACCGCGCAGTTCAACCAGCCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTGTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAg                                                                                                <  1:1461422/146‑1 (MQ=255)
                                                     ttCCCCGCGCAGTTCAACCAGCCCCTCTTCCAGACCCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAg                                                                                          <  1:613542/150‑1 (MQ=255)
                                                         ccGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGACAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGc                                                                                     >  2:69308/1‑151 (MQ=255)
                                                          cgcgCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCt                                                                                    >  1:11946/1‑151 (MQ=255)
                                                          cgcgCAGTTCAACCAGGCCCTCTCCCAGACGCTGGCAATAAAGCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGCCCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCt                                                                                    <  2:262353/151‑1 (MQ=255)
                                                           gcgcAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTg                                                                                   >  1:293324/1‑151 (MQ=255)
                                                               aGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGc                                                                               >  1:1292123/1‑151 (MQ=255)
                                                                 ttCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGcc                                                                              >  2:35906/1‑150 (MQ=255)
                                                                   cacccAGGCCCTTTTCCAGACGCTTGCGATAAACCGGACCGTCGGAGAGTTGGGAGTTGAACAGCATGGTAACCAGGGAGGAAACACCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCa                                                                             <  2:1635560/146‑1 (MQ=255)
                                                                    aaCCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATg                                                                           >  2:1518277/1‑150 (MQ=255)
                                                                        aGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCtt                                                                      >  2:721389/1‑151 (MQ=255)
                                                                         ggCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCtt                                                                      <  2:420389/150‑1 (MQ=255)
                                                                         ggCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGAACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTc                                                                     <  2:1198251/151‑1 (MQ=255)
                                                                             ctctTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATc                                                                 >  1:1376309/1‑151 (MQ=255)
                                                                              tctTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATc                                                                 >  2:1644205/1‑150 (MQ=255)
                                                                                 tCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACgg                                                             >  2:403281/1‑151 (MQ=255)
                                                                                  ccAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACgg                                                             <  2:1044411/150‑1 (MQ=255)
                                                                                  ccAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACgg                                                             >  1:1593388/1‑150 (MQ=255)
                                                                                   cAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGa                                                            >  1:926880/1‑150 (MQ=255)
                                                                                    aGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATGGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACgg                                                             <  1:1538204/148‑1 (MQ=255)
                                                                                      aCGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGa                                                         <  1:540414/150‑1 (MQ=255)
                                                                                      aCGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTGAAACAGCATAGTGCCCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGa                                                         <  1:176648/150‑1 (MQ=255)
                                                                                       cGCTTGCGATAAATCGGCTCGTCAGAGAGTTTGGGGTTTAACAGCAAAGTGACCAGGGAGGAAACCCCCAGAACCGGCAGCAGGGGGGACGGGATGACCACGGAGAACAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGa                                                            <  1:1519351/146‑1 (MQ=255)
                                                                                       cGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGAc                                                        >  2:1362495/1‑150 (MQ=255)
                                                                                       cGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGAc                                                        >  1:947036/1‑150 (MQ=255)
                                                                                       cGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGAc                                                        >  1:37956/1‑150 (MQ=255)
                                                                                       cGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTGGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAg                                                          <  1:1508648/148‑1 (MQ=255)
                                                                                        gCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACAt                                                      <  2:936050/151‑1 (MQ=255)
                                                                                         cTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATg                                                     >  2:724399/1‑151 (MQ=255)
                                                                                          ttGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGt                                                    >  2:1566468/1‑151 (MQ=255)
                                                                                           tGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGt                                                    >  1:10026/1‑150 (MQ=255)
                                                                                            gCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTa                                                   >  1:1615669/1‑150 (MQ=255)
                                                                                             cGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTaa                                                  >  1:240405/1‑150 (MQ=255)
                                                                                              gATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAAc                                                >  2:1225631/1‑151 (MQ=255)
                                                                                               aTAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAAcc                                               >  1:677680/1‑151 (MQ=255)
                                                                                                 aaaTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCAc                                             >  1:506001/1‑151 (MQ=255)
                                                                                                  aaTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACt                                            >  1:1191035/1‑151 (MQ=255)
                                                                                                  aaTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACt                                            <  2:301635/151‑1 (MQ=255)
                                                                                                  aaTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACt                                            >  1:1430205/1‑151 (MQ=255)
                                                                                                   aTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTg                                           >  1:1498954/1‑151 (MQ=255)
                                                                                                     cGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGcc                                         >  1:1590066/1‑151 (MQ=255)
                                                                                                      ggATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCg                                        >  1:149343/1‑151 (MQ=255)
                                                                                                       gATCGTCAGAGAGTTTGGAGTGGAACAGCATAGTGCCCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGc                                       <  1:952481/151‑1 (MQ=255)
                                                                                                          cGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCt                                      <  2:1139093/149‑1 (MQ=255)
                                                                                                             cAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTg                                     >  2:1124762/1‑147 (MQ=255)
                                                                                                                agagTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTgg                              >  1:560175/1‑151 (MQ=255)
                                                                                                                agagTTGGGATTGGAACAGCATAGTGACCAGGGAGGACACCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTgaga                                  <  1:1270008/147‑1 (MQ=255)
                                                                                                                 gagTTTGGAGTTGAACAGCATGGTGACCAGGGAGGACACCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTg                               <  1:1641149/149‑1 (MQ=255)
                                                                                                                  attttGGAGTTGACCAGCATAGTGCCCAGGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCg                            <  2:630006/149‑1 (MQ=255)
                                                                                                                  agTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCg                            >  2:1377950/1‑151 (MQ=255)
                                                                                                                    tttGGAGTTGAACAGTTTAGTGACCAGGGAGGACACCCCCAAAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTga                                    <  1:1473142/141‑1 (MQ=255)
                                                                                                                    tttGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGAt                          >  2:1673969/1‑151 (MQ=255)
                                                                                                                    ttgggAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCg                            <  2:572560/146‑1 (MQ=255)
                                                                                                                     ttGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGAt                          <  1:328845/150‑1 (MQ=255)
                                                                                                                      tGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGc                        >  2:893835/1‑151 (MQ=255)
                                                                                                                       ggAGTTGACCAGCATACGGCCCCGGTAGGACACCCCAAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGGCCTTCCCTCACGGAAGACATGTAACCCACTGCCGCTGAGATTGGCGATGc                        <  1:304477/150‑1 (MQ=255)
                                                                                                                       ggAGTTGAACAGCATGGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATg                         <  1:1295180/149‑1 (MQ=255)
                                                                                                                       ggAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCg                       >  2:90264/1‑151 (MQ=255)
                                                                                                                       ggAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCg                       <  1:1449848/151‑1 (MQ=255)
                                                                                                                       ggAGTTAAACAGCATAGTGACCAGGGAGGCAACCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCg                       <  1:943720/151‑1 (MQ=255)
                                                                                                                         aGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGt                     >  2:751141/1‑151 (MQ=255)
                                                                                                                           ttGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTg                    >  2:1288056/1‑150 (MQ=255)
                                                                                                                             gAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATc                 >  1:1437142/1‑151 (MQ=255)
                                                                                                                              aaCAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGa                   <  1:1603376/148‑1 (MQ=255)
                                                                                                                                cAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGc               >  2:630527/1‑150 (MQ=255)
                                                                                                                                cAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGc               >  1:1008786/1‑150 (MQ=255)
                                                                                                                                   cATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATc           >  1:39291/1‑151 (MQ=255)
                                                                                                                                         gACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGcgcg     >  2:1684459/1‑151 (MQ=255)
                                                                                                                                          aCCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTgcgc      <  2:921775/149‑1 (MQ=255)
                                                                                                                                             agggaggaCATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAt  >  1:366700/1‑150 (MQ=255)
                                                                                                                                             agggaggaCATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAt  >  2:1218172/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
AGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAT  >  NC_000913/4366079‑4366369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑