Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,617,393 A→G D24G (GAC→GGC)  deoC → 2‑deoxyribose‑5‑phosphate aldolase, NAD(P)‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,617,3930AG93.9% 330.1 / NA 115D24G (GAC→GGC) deoC2‑deoxyribose‑5‑phosphate aldolase, NAD(P)‑linked
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (55/53);  minor base C (0/7);  total (55/60)
Fisher's exact test for biased strand distribution p-value = 1.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

AGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACG  >  NC_000913/4617245‑4617538
                                                                                                                                                    |                                                                                                                                                 
aGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGgca                                                                                                                                                 <  2:1716770/151‑1 (MQ=255)
 gtgtgtTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGgcac                                                                                                                                                >  2:309812/1‑151 (MQ=255)
 gtgtgtTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGgca                                                                                                                                                 >  2:676283/1‑150 (MQ=255)
     gtTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACcgac                                                                                                                                            <  1:164271/151‑1 (MQ=255)
        gCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACgag                                                                                                                                         <  2:1255080/151‑1 (MQ=255)
        gCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACgag                                                                                                                                         <  1:373547/151‑1 (MQ=255)
            aGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGaa                                                                                                                                       >  1:1339769/1‑149 (MQ=255)
             gTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGt                                                                                                                                    <  2:1297386/151‑1 (MQ=255)
              tAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTg                                                                                                                                   >  2:1103833/1‑151 (MQ=255)
               aGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTg                                                                                                                                   <  1:159398/150‑1 (MQ=255)
               aGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTg                                                                                                                                   >  2:867643/1‑150 (MQ=255)
                 aTGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATc                                                                                                                                <  2:1283655/151‑1 (MQ=255)
                  agTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCg                                                                                                                               >  1:780722/2‑151 (MQ=255)
                    ttAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGcc                                                                                                                             >  1:1222384/1‑151 (MQ=255)
                     tAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGccc                                                                                                                            >  2:776511/1‑151 (MQ=255)
                      aGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCt                                                                                                                           >  2:350381/1‑151 (MQ=255)
                       gAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCtg                                                                                                                          <  1:758289/151‑1 (MQ=255)
                         aTACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCtgtg                                                                                                                        <  2:49400/151‑1 (MQ=255)
                         aTACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAACGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCtgtg                                                                                                                        <  1:878797/151‑1 (MQ=255)
                          tACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTgtgt                                                                                                                       >  1:810408/1‑151 (MQ=255)
                           aCTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTgtgt                                                                                                                       <  2:440156/150‑1 (MQ=255)
                              aaCAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGtca                                                                                                                     <  1:1535347/149‑1 (MQ=255)
                               aCAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGtcatc                                                                                                                   >  2:329843/1‑150 (MQ=255)
                                cAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGtcatc                                                                                                                   >  2:214716/1‑149 (MQ=255)
                                cAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGtcatc                                                                                                                   >  1:705023/1‑149 (MQ=255)
                                cAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAg                                                                                                                 <  1:697045/151‑1 (MQ=255)
                                  aaCTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGc                                                                                                               <  1:1620913/151‑1 (MQ=255)
                                         aaGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAAc                                                                                                         >  2:873587/1‑150 (MQ=255)
                                         aaGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACt                                                                                                        >  2:1182209/1‑151 (MQ=255)
                                         aaGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGAACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACt                                                                                                        >  2:1097447/1‑151 (MQ=255)
                                            gTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCg                                                                                                     <  1:995042/151‑1 (MQ=255)
                                               aaTTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTc                                                                                                  >  2:1607071/1‑151 (MQ=255)
                                               aaTTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTc                                                                                                  <  2:1057577/151‑1 (MQ=255)
                                                aTTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCg                                                                                                 <  1:1685325/151‑1 (MQ=255)
                                                  tttATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCgg                                                                                                <  2:1194331/150‑1 (MQ=255)
                                                  tttATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGc                                                                                               >  2:250506/1‑151 (MQ=255)
                                                       tGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATa                                                                                           <  2:504327/150‑1 (MQ=255)
                                                        ggCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATAcc                                                                                         >  2:534028/1‑151 (MQ=255)
                                                        ggCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATAcc                                                                                         <  1:1244769/151‑1 (MQ=255)
                                                        ggCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATAcc                                                                                         <  1:1530952/151‑1 (MQ=255)
                                                           gACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATAccgcc                                                                                      >  1:1054261/1‑151 (MQ=255)
                                                           gACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATAccgcc                                                                                      >  1:1397240/1‑151 (MQ=255)
                                                              aaGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACcgccgc                                                                                    <  2:571534/150‑1 (MQ=255)
                                                                      agaATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCCCCCTGAATGACGACGGCCCCGACGAGAAAGTGATCGCCCTGTGTCACCAGGCCAAAACTCCGGTCGGAAATACCGCCGCTATCTGtat                                                                           <  1:304948/151‑1 (MQ=255)
                                                                         atgaaatgaCTGATCTGAAAGCAAGCAGCCGGGGTGCACTAAAATTGATGGCCCTGACCCCCCTGAATGACGACGGCCCCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGtatcta                                                                        <  2:1284402/151‑1 (MQ=255)
                                                                           gaaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGtatctatc                                                                      >  1:1575490/1‑151 (MQ=255)
                                                                            aaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCCCCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCAAAAACTCCGGTCGGAAATACCGCCGCTATCTGTATCTATcc                                                                     <  2:245382/151‑1 (MQ=255)
                                                                              atgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTc                                                                   >  1:381027/1‑151 (MQ=255)
                                                                              atgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTAAAATTGATGGCCCTGACCCCCCTGAATGACGACGGCACCGACGAGAAAGTGACCGCCCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTc                                                                   <  2:476843/151‑1 (MQ=255)
                                                                               tgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCg                                                                  >  2:1182653/1‑151 (MQ=255)
                                                                                gaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGc                                                                 >  2:757847/1‑151 (MQ=255)
                                                                                 aCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCt                                                                >  2:426151/1‑151 (MQ=255)
                                                                                  cTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCtt                                                               >  1:1057244/1‑151 (MQ=255)
                                                                                  cTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCtt                                                               >  1:188863/1‑151 (MQ=255)
                                                                                    gATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCttt                                                              >  2:482597/1‑150 (MQ=255)
                                                                                      tCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATc                                                           >  1:52031/1‑151 (MQ=255)
                                                                                          aaaGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGCCCCCCCTGAATGACGACGGCCCCGACGAGAAAGTGATCGCCCTGTGTCATCAGCCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTa                                                             <  2:1565270/145‑1 (MQ=255)
                                                                                          aaaGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCCCCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCACCAGCCAAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATccc                                                         <  1:1366128/149‑1 (MQ=255)
                                                                                          aaaGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCg                                                        >  1:691481/1‑150 (MQ=255)
                                                                                           aagcaagcAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAt                                                      >  2:725891/1‑151 (MQ=255)
                                                                                           aagcaagcAGCCTGCGTGCACTGAAATTGATGGACCGGACCCCCCTGAATGCCGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCAAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAt                                                      <  1:194866/151‑1 (MQ=255)
                                                                                             gcaagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGtatctat                                                                       >  2:1626015/1‑132 (MQ=255)
                                                                                               aagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCt                                                  >  1:642478/1‑151 (MQ=255)
                                                                                               aagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCt                                                  >  2:1385428/1‑151 (MQ=255)
                                                                                               aagcaCCCTGCGTGCAAGAAAATTGATGGACTGCCCCCCCCGGAATGCCGCCGGCCCCGACGAGAAAGTGATCCCCTTGTGTCATCAGGCCAAACCCCCGGTCGCAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAtt                                                     <  1:912199/148‑1 (MQ=255)
                                                                                                 gcagcCTGCGTGCATTGAAATTGATGGACCTGCCCACCCTGAATGACGACGCCACCGACGAAAAAGGGATCGCCTTGTGTCATCAGCCAAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTc                                                 <  1:1198411/150‑1 (MQ=255)
                                                                                                   agcCTGCTTGCACTGAAATTGATGCTCCTGACCTCCCTAACGGACGCCGCCCCGGACGATAAAGTGGTCGTCTTGTATCATCAGGCAAAAACCCCTGCCGGCATTCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTc                                                 <  2:6377/148‑1 (MQ=255)
                                                                                                   agcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGc                                               >  2:1031387/1‑150 (MQ=255)
                                                                                                     ccTGCGTGCACTGAAATTGATGGACCTGCCCCCCCTGAATGACGCCGGCCCCGACGAAAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCaaa                                            <  1:1448851/151‑1 (MQ=255)
                                                                                                      cTGCGTGCACTGAAATTGATGGACCCGACCCCCCTAAATGACGCCGGCCGCGACGAAAAAGTGATCGCCTTGTGTCATCAGCCCAAACCTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCaa                                             <  1:676225/149‑1 (MQ=255)
                                                                                                       tGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAAc                                          >  2:1449659/1‑151 (MQ=255)
                                                                                                        gCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCTCAAAAct                                         >  1:1465869/1‑151 (MQ=255)
                                                                                                          gTGCACTGAAATTGATGGACCTGACCCCCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAAc                                          <  2:1112789/148‑1 (MQ=255)
                                                                                                          gTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAActct                                       >  2:37968/1‑151 (MQ=255)
                                                                                                           tGCACTGAAATTGATGGACCTGACCACCCTGAATGACGCCGGCCCCGACGAGAAAGTTATCGCCCTGTGTCATCAGACAAAACCTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAActct                                       <  1:370647/150‑1 (MQ=255)
                                                                                                           tGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTg                                      >  1:1480306/1‑151 (MQ=255)
                                                                                                           tGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTg                                      >  1:1360549/1‑151 (MQ=255)
                                                                                                             cACGGAAATTGAGGGCCCTGACCCCCCTGAATGACGACGCCCCCGAGGAGAAAGTGATCCCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTg                                      <  2:1425976/149‑1 (MQ=255)
                                                                                                              aCTGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGaa                                    >  1:1591187/1‑150 (MQ=255)
                                                                                                               cTGAAATTGATGGCCCTGACCCCCCTGAATGCCGACGCCCCCGACGAGAAAGTGACCGCCCTGTGTCACCAGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGaaa                                   <  1:1132620/150‑1 (MQ=255)
                                                                                                                tGAAATTGATGGACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGaa                                    >  2:1515840/1‑148 (MQ=255)
                                                                                                                  aaaTTGATGGCCCTGACCCCCCTGAATGACGCCGGCCCCGACGAGAAAGTGACCGCCCTGTGTCATCGGGCCAAACCTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAaga                                 <  1:1694785/149‑1 (MQ=255)
                                                                                                                   aaTTGATGGACCTGACCCCCCTGAATGACGACGCCCCCGACGAGAAAGTGACCGCCCTGTGTCATCAGGCAAAAACCCCGGTCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCa                              <  1:449134/151‑1 (MQ=255)
                                                                                                                       gATGGCCCTGCCCCCCCTAAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAgg                            <  1:507853/149‑1 (MQ=255)
                                                                                                                          ggcccTGACCCCCCTGAATGCCGACGGCCCGGACGAGAAAGTGATCGCCCTGTGTCATCAGGCAAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAgg                            <  2:93545/143‑1 (MQ=255)
                                                                                                                           gACCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCAccc                      >  2:1565692/1‑151 (MQ=255)
                                                                                                                            aCCTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAActct                                       >  2:215316/1‑133 (MQ=255)
                                                                                                                             ccTGCCCCCCCTGAATGACGACGGCACCGACGAAAAAGTGATCGCCCTGTGTCACCAGGCAAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCAccc                      <  2:155197/149‑1 (MQ=255)
                                                                                                                             ccTGACCCCCCTGAATGACGACGGCCCCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAActct                                       <  1:215298/132‑1 (MQ=255)
                                                                                                                             ccTGACCACCCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCg                    >  1:343933/1‑151 (MQ=255)
                                                                                                                                  ccccccTGAATGACGACGGCCCCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAACCTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGaa                 <  1:260306/146‑1 (MQ=255)
                                                                                                                                    ccccTGAATGACGACGCCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATcc             <  1:142148/150‑1 (MQ=255)
                                                                                                                                    accCTGAATGACGACGGCACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATcc             >  2:1039578/1‑151 (MQ=255)
                                                                                                                                      ccTGAATGACGCCGCCCCCGACGAGAAAGTGATCGCCCTGTGTCATCAGCCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGt           <  1:602147/151‑1 (MQ=255)
                                                                                                                                      ccTGAAGGACGACGGCACCGACGAAAAAGTGATCGCCTTGTGTCATCAGGCCAAAACCCCGGCCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGt           <  1:1256450/151‑1 (MQ=255)
                                                                                                                                         aaaTGACGCCGGCACCGACGAAAAAGTGATCGCCCTGTGTCATCAGCCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTAt         <  1:97772/149‑1 (MQ=255)
                                                                                                                                          aaggacgacggcACCGACGAGAAAGTGACCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCg       <  1:73875/148‑1 (MQ=255)
                                                                                                                                          aaTGACGGCGGCGCCGACGAAAAGGTGTTCGCCCTGTTTCACCAGCCCAAACCTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATc        <  2:1627554/150‑1 (MQ=255)
                                                                                                                                            tGACGACGGCACCGACGAAAAAGTGACCCCCCTGTGTCATCAGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGc      <  1:469371/150‑1 (MQ=255)
                                                                                                                                               cgacggcACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACg  >  1:1588578/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACG  >  NC_000913/4617245‑4617538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑