Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 758,165 T→C C159C (TGT→TGC sdhB → succinate dehydrogenase, FeS subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913758,1650TC98.2% 404.3 / ‑1.3 110C159C (TGT→TGCsdhBsuccinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (57/51);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

Only 100 of 113 total aligned reads displayed.

TCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAG  >  NC_000913/758017‑758311
                                                                                                                                                    |                                                                                                                                                  
tCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGccc                                                                                                                                                  >  1:384007/1‑151 (MQ=255)
tCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGccc                                                                                                                                                  >  1:858164/1‑151 (MQ=255)
 cTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCg                                                                                                                                                 >  2:1271625/1‑151 (MQ=255)
   aTGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTc                                                                                                                                               >  2:248938/1‑151 (MQ=255)
       gcAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTc                                                                                                                                           >  1:1388150/1‑151 (MQ=255)
        cAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTc                                                                                                                                           <  1:738160/150‑1 (MQ=255)
             tGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCtggtgg                                                                                                                                     >  2:1711484/1‑151 (MQ=255)
               agaAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGa                                                                                                                                    >  1:582137/1‑150 (MQ=255)
                gaAAATTAAGTCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAAt                                                                                                                                  <  1:1133244/151‑1 (MQ=255)
                gaAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAAt                                                                                                                                  >  1:374279/1‑151 (MQ=255)
                gaAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAAt                                                                                                                                  <  1:121701/151‑1 (MQ=255)
                 aaaaTTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATc                                                                                                                                 <  2:459652/151‑1 (MQ=255)
                     ttAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCg                                                                                                                              >  2:231220/1‑150 (MQ=255)
                      tAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGAt                                                                                                                            >  2:1115916/1‑151 (MQ=255)
                       aaGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGAt                                                                                                                            >  2:855078/1‑150 (MQ=255)
                             tACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGttt                                                                                                                      <  2:859329/150‑1 (MQ=255)
                              aCCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTa                                                                                                                     <  1:1487550/150‑1 (MQ=255)
                              aCCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTAt                                                                                                                    <  2:1634045/151‑1 (MQ=255)
                                 tGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCgg                                                                                                                 >  1:1485890/1‑151 (MQ=255)
                                    tGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGccc                                                                                                              >  2:194968/1‑151 (MQ=255)
                                      aataatGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCgg                                                                                                            >  2:653265/1‑151 (MQ=255)
                                       ataatGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGc                                                                                                           <  2:1450065/151‑1 (MQ=255)
                                        taatGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGc                                                                                                           >  1:67669/1‑150 (MQ=255)
                                         aatGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCa                                                                                                          <  1:1478712/150‑1 (MQ=255)
                                         aatGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAg                                                                                                         <  1:1009262/151‑1 (MQ=255)
                                           tGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCgg                                                                                                            >  1:764630/1‑146 (MQ=255)
                                              aCAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCtt                                                                                                     >  2:649820/1‑150 (MQ=255)
                                                aaaaTCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGtt                                                                                                  <  1:1552359/151‑1 (MQ=255)
                                                  aaTCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGtt                                                                                                  >  2:1400811/1‑149 (MQ=255)
                                                     ccGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCgg                                                                                             >  2:162909/1‑151 (MQ=255)
                                                     ccGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCgg                                                                                             >  1:787308/1‑151 (MQ=255)
                                                      cGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGc                                                                                            <  2:756702/151‑1 (MQ=255)
                                                       gCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCa                                                                                           <  2:695572/151‑1 (MQ=255)
                                                         cAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCat                                                                                          >  2:1361128/1‑150 (MQ=255)
                                                         cAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCat                                                                                          >  1:290766/1‑150 (MQ=255)
                                                           gCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGGCTGtttgtgg                                                                                             >  2:1542918/1‑140 (MQ=255)
                                                            cTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCCGGCTTGTTAGCGGCatat                                                                                        >  2:17995/1‑149 (MQ=255)
                                                             tCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCg                                                                                      >  2:741503/1‑150 (MQ=255)
                                                               gcgAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGtt                                                                                    <  1:623805/150‑1 (MQ=255)
                                                               gcgAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGGTAGCGGCATATCGttt                                                                                   >  1:421126/1‑151 (MQ=255)
                                                                 gAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTcc                                                                                 <  1:83000/151‑1 (MQ=255)
                                                                     aTTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTTTTAGCGGCATATCGTTTTCtgat                                                                             >  1:1308370/1‑151 (MQ=255)
                                                                     aTTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTATCGGCATATCCTTTTCt                                                                                >  1:9707/1‑148 (MQ=255)
                                                                     aTTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgat                                                                             >  1:203442/1‑151 (MQ=255)
                                                                     aTTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgat                                                                             <  1:465455/151‑1 (MQ=255)
                                                                      tttACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgatt                                                                            >  2:1510485/1‑151 (MQ=255)
                                                                         aCAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat                                                                         <  2:1037550/151‑1 (MQ=255)
                                                                         aCAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat                                                                         <  1:1271530/151‑1 (MQ=255)
                                                                         aCAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat                                                                         <  2:543555/151‑1 (MQ=255)
                                                                          cAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATa                                                                        <  1:390439/151‑1 (MQ=255)
                                                                               gCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCg                                                                    >  2:546516/1‑150 (MQ=255)
                                                                               gCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCg                                                                    <  2:1387949/150‑1 (MQ=255)
                                                                                   gagCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATa                                                               <  2:941976/151‑1 (MQ=255)
                                                                                      cagcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCg                                                            <  1:1540302/151‑1 (MQ=255)
                                                                                       agcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCga                                                           <  1:1212698/151‑1 (MQ=255)
                                                                                           cgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACt                                                       <  1:855011/151‑1 (MQ=255)
                                                                                            gAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGGCTGTTAGCGGCTTAATGTTTTCTGATTGCTAGCCGTgtttacggaaatg                                                      >  1:284469/1‑139 (MQ=255)
                                                                                               aaaCTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACa                                                   <  2:346959/151‑1 (MQ=255)
                                                                                               aaaCTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACa                                                   <  2:256631/151‑1 (MQ=255)
                                                                                                aaCTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACaa                                                  <  1:1126130/151‑2 (MQ=255)
                                                                                                aaCTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAg                                                  <  2:19184/151‑1 (MQ=255)
                                                                                                aaCTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAg                                                  <  2:623037/151‑1 (MQ=255)
                                                                                                   tCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGGGACTGACAgcc                                                >  2:1584041/1‑150 (MQ=255)
                                                                                                    cGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAgccgc                                              <  1:1711368/151‑1 (MQ=255)
                                                                                                    cGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAgccg                                               <  1:654741/150‑1 (MQ=255)
                                                                                                    cGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAgccg                                               <  1:280329/150‑1 (MQ=255)
                                                                                                     gACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAgccgcc                                             >  1:729650/1‑151 (MQ=255)
                                                                                                       cGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTc                                           <  1:1283489/151‑1 (MQ=255)
                                                                                                         ggCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTTTCGGGATATTGTTTGCTGGTTTATAGCCGTGATACCCAGACTGGCAACCGCCt                                            >  2:111103/1‑148 (MQ=255)
                                                                                                         ggCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGa                                         <  2:787374/151‑1 (MQ=255)
                                                                                                          gCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGa                                         >  1:393880/1‑150 (MQ=255)
                                                                                                          gCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGGGATACCGAGAATGACAGCCGCCTCg                                          >  2:1074168/1‑149 (MQ=255)
                                                                                                           cTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAc                                        >  1:165050/1‑150 (MQ=255)
                                                                                                             gTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACg                                       <  1:360073/149‑1 (MQ=255)
                                                                                                              tATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACgg                                      >  1:111892/1‑149 (MQ=255)
                                                                                                               aTGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGGTTGTTAGCGGCATATCGGTTCCTGAATGATAGCCGTGATAACGCGGATGACAGGCGCCTCGGCGGttt                                   >  2:1283102/1‑151 (MQ=255)
                                                                                                               aTGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGttt                                   <  1:222515/151‑1 (MQ=255)
                                                                                                               aTGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGtt                                    <  1:873355/150‑1 (MQ=255)
                                                                                                                 gAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGa                                 <  2:306371/151‑1 (MQ=255)
                                                                                                                 gAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGCGATACCGAGACTGACAGCCGCCTCGACGGgtt                                   >  2:823213/1‑149 (MQ=255)
                                                                                                                    tGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACggagatcgg                               >  2:677506/1‑143 (MQ=255)
                                                                                                                    tGTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACgg                                      <  1:677448/143‑1 (MQ=255)
                                                                                                                     gTATTCTCTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTTATAGCCCTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTgg                             >  1:945196/1‑150 (MQ=255)
                                                                                                                         tctcTGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGc                          <  1:1704842/150‑1 (MQ=255)
                                                                                                                           tctGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTTGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCAtt                       >  1:1031390/1‑151 (MQ=255)
                                                                                                                            ctGCGCATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCa                         <  1:649762/148‑1 (MQ=255)
                                                                                                                              gcgcATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg                    <  1:605395/151‑1 (MQ=255)
                                                                                                                               cgcATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGc                   >  2:398417/1‑151 (MQ=255)
                                                                                                                                 cATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCGTTCAGCg                  <  2:1388243/150‑1 (MQ=255)
                                                                                                                                 cATGTTGTTCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg                  <  2:384035/150‑1 (MQ=255)
                                                                                                                                     ttgttCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTAtt              <  1:509188/150‑1 (MQ=255)
                                                                                                                                       gttCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCg           <  1:838944/151‑1 (MQ=255)
                                                                                                                                        ttCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCg           <  1:1682441/150‑1 (MQ=255)
                                                                                                                                         tCAACCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGc          <  2:1410992/150‑1 (MQ=255)
                                                                                                                                           aaCCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCCCGACGGGTTGAGTGATGCATTCAGCGTATTTCGCTGt       >  1:132489/1‑151 (MQ=255)
                                                                                                                                            aCCTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGt       >  1:739419/1‑150 (MQ=255)
                                                                                                                                             ccTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATTCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTc      <  1:17993/150‑1 (MQ=255)
                                                                                                                                             ccTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGGGACTGACCGCCCCCTCGAAGGGTTTAGTGGTGCATTGAGCGGATTCCGCTGTc      >  1:1527957/1‑150 (MQ=255)
                                                                                                                                             ccTCTTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTca     <  1:1400718/151‑1 (MQ=255)
                                                                                                                                                ctTGCCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTcac    >  2:55803/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAG  >  NC_000913/758017‑758311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: