Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 218,942 A→G V218A (GTT→GCT)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913218,9420AG99.2% 426.4 / ‑4.0 117V218A (GTT→GCT) tsaAtRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (56/60);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 118 total aligned reads displayed.

GAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGA  >  NC_000913/218795‑219089
                                                                                                                                                    |                                                                                                                                                   
gAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGt                                                                                                                                                  >  2:119647/1‑151 (MQ=255)
 aaGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGtt                                                                                                                                                 >  2:675530/1‑151 (MQ=255)
 aaGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGtt                                                                                                                                                 >  1:642635/1‑151 (MQ=255)
    gTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTaa                                                                                                                                               >  1:1062093/1‑150 (MQ=255)
    gTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTaaaa                                                                                                                                             <  2:1562228/151‑1 (MQ=255)
       gAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATc                                                                                                                                           >  2:1623277/1‑151 (MQ=255)
       gAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAGGGATTTTGGTTAACAGGGCAAAAGGAATTTCAAAAGAGGAGGGCCGGAATTTAAAGCGGTTCCAGCGCAAAAACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAAt                                                                                                                                            >  1:1360528/1‑149 (MQ=255)
        aaCAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCa                                                                                                                                          <  1:1327148/151‑1 (MQ=255)
          cAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATg                                                                                                                                        >  2:1467680/1‑151 (MQ=255)
          cAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATg                                                                                                                                        <  1:655498/151‑1 (MQ=255)
           aGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGt                                                                                                                                       <  2:416947/151‑1 (MQ=255)
                cAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCa                                                                                                                                  <  2:1062174/151‑1 (MQ=255)
                cAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCa                                                                                                                                  <  1:1150195/151‑1 (MQ=255)
                 aaGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGcc                                                                                                                                   >  1:469963/1‑149 (MQ=255)
                 aaGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGcc                                                                                                                                   >  2:1268643/1‑149 (MQ=255)
                 aaGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAg                                                                                                                                 <  1:1157376/151‑1 (MQ=255)
                  aGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGa                                                                                                                                <  2:174126/151‑1 (MQ=255)
                    gCGAGCCTGGGACAAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGAc                                                                                                                               <  1:952253/151‑1 (MQ=255)
                      gAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGc                                                                                                                            <  2:449587/151‑1 (MQ=255)
                       aGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGc                                                                                                                            >  2:827333/1‑150 (MQ=255)
                       aGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCa                                                                                                                           <  1:357477/151‑1 (MQ=255)
                        gCCTGGGACAAAAAAAGTGAATTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCAt                                                                                                                          >  1:56685/1‑150 (MQ=255)
                         ccTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCAt                                                                                                                          >  2:1561819/1‑150 (MQ=255)
                         ccTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATa                                                                                                                         <  2:1445531/151‑1 (MQ=255)
                             ggACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGtt                                                                                                                     >  1:1308771/1‑151 (MQ=255)
                             ggACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGtt                                                                                                                     <  1:1363928/151‑1 (MQ=255)
                             ggACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGtt                                                                                                                     >  2:1579989/1‑151 (MQ=255)
                               aCAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGtttt                                                                                                                   >  1:892446/1‑151 (MQ=255)
                               aCAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGATCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGtttt                                                                                                                   <  1:358898/151‑1 (MQ=255)
                                  aaaaaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGc                                                                                                                 >  2:1513292/1‑151 (MQ=255)
                                   aaaaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc                                                                                                                >  1:1455155/1‑151 (MQ=255)
                                       aaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGt                                                                                                             >  2:1271699/1‑150 (MQ=255)
                                        aaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGttt                                                                                                           >  1:344/1‑151 (MQ=255)
                                             aTTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc                                                                                                       >  2:193130/1‑150 (MQ=255)
                                             aTTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc                                                                                                       >  1:966897/1‑150 (MQ=255)
                                             aTTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc                                                                                                       >  1:152879/1‑150 (MQ=255)
                                              tttAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCAc                                                                                                     >  2:788403/1‑151 (MQ=255)
                                              tttAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCAc                                                                                                     >  1:380647/1‑151 (MQ=255)
                                               ttAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCAc                                                                                                     <  1:1689313/150‑1 (MQ=255)
                                                  gTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCttt                                                                                                 <  1:1507715/151‑1 (MQ=255)
                                                    ttACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTa                                                                                                <  1:527521/150‑1 (MQ=255)
                                                    ttACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTa                                                                                                <  2:892515/150‑1 (MQ=255)
                                                     tACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACg                                                                                              >  2:22934/1‑151 (MQ=255)
                                                      aCCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGa                                                                                             <  2:1184265/151‑1 (MQ=255)
                                                       ccAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGAt                                                                                            <  2:1137345/151‑1 (MQ=255)
                                                        cAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGAt                                                                                            >  2:477195/1‑150 (MQ=255)
                                                        cAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATa                                                                                           <  1:633704/151‑1 (MQ=255)
                                                         aGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGAt                                                                                            >  2:459072/1‑149 (MQ=255)
                                                         aGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAg                                                                                          <  1:890211/151‑1 (MQ=255)
                                                         aGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAg                                                                                          <  2:1405941/151‑1 (MQ=255)
                                                            gCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGc                                                                                        <  1:1393783/150‑1 (MQ=255)
                                                              aaaaGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCgg                                                                                     >  2:1225559/1‑151 (MQ=255)
                                                               aaaGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCggg                                                                                    <  1:119639/151‑1 (MQ=255)
                                                                aaGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGgc                                                                                   >  1:1310640/1‑151 (MQ=255)
                                                                 aGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGgcg                                                                                  >  2:842749/1‑151 (MQ=255)
                                                                   aaaTGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg                                                                                >  2:642570/1‑151 (MQ=255)
                                                                   aaaTGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg                                                                                >  1:1689658/1‑151 (MQ=255)
                                                                    aaTGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCgg                                                                               <  1:125997/151‑1 (MQ=255)
                                                                      tGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGt                                                                             >  1:21819/1‑151 (MQ=255)
                                                                       gTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGt                                                                             <  2:1112457/150‑1 (MQ=255)
                                                                         cAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTcc                                                                           <  1:1098108/150‑1 (MQ=255)
                                                                         cAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCt                                                                          <  1:89176/151‑1 (MQ=255)
                                                                           aaaGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTgc                                                                        >  1:1607561/1‑151 (MQ=255)
                                                                              gagaAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGcc                                                                     >  1:14556/1‑151 (MQ=255)
                                                                                    ggCGTGAATTTAACGCGGTTCCGGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACt                                                               <  2:866005/151‑1 (MQ=255)
                                                                                    ggCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACt                                                               >  2:892535/1‑151 (MQ=255)
                                                                                         gAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTcgcg                                                          <  1:1697958/151‑1 (MQ=255)
                                                                                         gAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTcgc                                                           <  2:1520599/150‑1 (MQ=255)
                                                                                           aTTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGa                                                        <  1:551704/151‑1 (MQ=255)
                                                                                                   cgGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGc                                                >  1:1399707/1‑151 (MQ=255)
                                                                                                   cgGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGc                                                <  2:138759/151‑1 (MQ=255)
                                                                                                    ggTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCg                                               <  2:1142217/151‑1 (MQ=255)
                                                                                                       tCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGtt                                             >  2:290838/1‑150 (MQ=255)
                                                                                                          aGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACt                                         >  1:1419588/1‑151 (MQ=255)
                                                                                                          aGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACt                                         >  1:1477407/1‑151 (MQ=255)
                                                                                                           gcgcAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTg                                        <  2:1215912/151‑1 (MQ=255)
                                                                                                            cgcAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGc                                       <  2:545327/151‑1 (MQ=255)
                                                                                                             gcAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCg                                      <  2:1302459/151‑1 (MQ=255)
                                                                                                             gcAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCg                                      <  1:803123/151‑1 (MQ=255)
                                                                                                              cAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCgg                                     >  2:318255/1‑151 (MQ=255)
                                                                                                                aaGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCCCCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGAt                                   <  2:220260/151‑1 (MQ=255)
                                                                                                                      tCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCt                             >  1:1049549/1‑151 (MQ=255)
                                                                                                                       cAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCtt                            <  1:702286/151‑1 (MQ=255)
                                                                                                                         aaaCCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTtct                          >  2:1387342/1‑151 (MQ=255)
                                                                                                                          aaCCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTtctc                         <  2:1625542/151‑1 (MQ=255)
                                                                                                                             cTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTcc                        >  1:384005/1‑149 (MQ=255)
                                                                                                                              tGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGc                     >  1:531950/1‑151 (MQ=255)
                                                                                                                               gCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGc                     <  2:470006/150‑1 (MQ=255)
                                                                                                                                       gACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAg             <  2:752885/150‑1 (MQ=255)
                                                                                                                                        aCGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgct           <  2:344/151‑1 (MQ=255)
                                                                                                                                         cgcgCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgctg          <  1:647390/151‑1 (MQ=255)
                                                                                                                                          gcgcCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCGAAAg             >  2:172547/1‑147 (MQ=255)
                                                                                                                                          gcgcCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgctgc         <  1:1678948/151‑1 (MQ=255)
                                                                                                                                            gcCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtt       <  1:1561724/151‑1 (MQ=255)
                                                                                                                                             ccAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCttt      <  1:375219/151‑1 (MQ=255)
                                                                                                                                              cAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtttt     <  2:424551/151‑1 (MQ=255)
                                                                                                                                               agcgagcgTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTc    <  2:152888/151‑1 (MQ=255)
                                                                                                                                               aGCGAGTGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTc    >  2:366932/1‑151 (MQ=255)
                                                                                                                                                gCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCg   >  2:1109197/1‑151 (MQ=255)
                                                                                                                                                 cGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTGTTc    <  1:1467582/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGA  >  NC_000913/218795‑219089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: