Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,226,979 A→G intergenic (+11/+92) ycgJ → / ← pliG uncharacterized protein/periplasmic inhibitor of g‑type lysozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,226,9790AG97.9% 539.4 / 0.4 140intergenic (+11/+92)ycgJ/pliGuncharacterized protein/periplasmic inhibitor of g‑type lysozyme
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (65/72);  total (67/73)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01

Only 100 of 140 total aligned reads displayed.

TTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTC  >  NC_000913/1226832‑1227124
                                                                                                                                                   |                                                                                                                                                 
ttACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCAt                                                                                                                                                >  2:66600/1‑151 (MQ=255)
ttACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCAt                                                                                                                                                >  2:691671/1‑151 (MQ=255)
   cATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTAt                                                                                                                                             <  2:1687963/151‑1 (MQ=255)
     ttCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGt                                                                                                                                           >  1:64912/1‑151 (MQ=255)
      tCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGt                                                                                                                                           <  2:727103/150‑1 (MQ=255)
       cGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGtt                                                                                                                                          <  1:1575411/150‑1 (MQ=255)
        gCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCt                                                                                                                                        >  1:1000518/1‑151 (MQ=255)
           aaCGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTa                                                                                                                                       <  1:1252301/149‑1 (MQ=255)
           aaCGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTAtt                                                                                                                                     >  2:531936/1‑151 (MQ=255)
           aaCGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAAATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTAt                                                                                                                                      <  1:1661197/150‑1 (MQ=255)
             cGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCg                                                                                                                                   <  1:866814/151‑1 (MQ=255)
             cGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCg                                                                                                                                   >  2:1486917/1‑151 (MQ=255)
              ggTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGt                                                                                                                                  <  1:1313834/151‑1 (MQ=255)
                   aTTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCAtt                                                                                                                              <  1:1493775/150‑1 (MQ=255)
                    ttttGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTa                                                                                                                             >  2:126820/1‑150 (MQ=255)
                           aTGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTg                                                                                                                     >  2:1254747/1‑151 (MQ=255)
                            tGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTg                                                                                                                     >  2:1303747/1‑150 (MQ=255)
                             gTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAg                                                                                                                   <  1:1128186/151‑1 (MQ=255)
                                   gAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACag                                                                                                             <  1:1426020/151‑1 (MQ=255)
                                    aaaaaTTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACag                                                                                                             >  1:663072/1‑149 (MQ=255)
                                      aaaTTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACagaga                                                                                                          <  2:16971/151‑1 (MQ=255)
                                      aaaTTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACag                                                                                                             >  2:919237/1‑148 (MQ=255)
                                          tATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAgagg                                                                                                        >  2:563766/1‑149 (MQ=255)
                                          tATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAgagg                                                                                                        >  2:142946/1‑149 (MQ=255)
                                           aTGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAgaggag                                                                                                      <  2:1059889/150‑1 (MQ=255)
                                           aTGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGt                                                                                                     <  2:602151/151‑1 (MQ=255)
                                            tGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTg                                                                                                    >  1:492648/1‑151 (MQ=255)
                                             gTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTgg                                                                                                   >  2:188442/1‑151 (MQ=255)
                                              tCGTGATGATCGTTACTTTGGTGTGGATGGCAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTggg                                                                                                  >  2:1166282/1‑151 (MQ=255)
                                                   atgatCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCAtt                                                                                             >  1:1178704/1‑151 (MQ=255)
                                                     gatCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTa                                                                                            <  2:499292/150‑1 (MQ=255)
                                                      atCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAAc                                                                                          >  2:1138581/1‑151 (MQ=255)
                                                      atCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAAc                                                                                          <  1:508235/151‑1 (MQ=255)
                                                      atCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAAc                                                                                          >  2:1668761/1‑151 (MQ=255)
                                                      atCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAAc                                                                                          <  2:938965/151‑1 (MQ=255)
                                                      atCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAAc                                                                                          >  1:1632488/1‑151 (MQ=255)
                                                       tCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACg                                                                                         >  1:227572/1‑151 (MQ=255)
                                                       tCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACg                                                                                         >  2:483822/1‑151 (MQ=255)
                                                       tCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACg                                                                                         >  2:1377682/1‑151 (MQ=255)
                                                        cGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGt                                                                                        >  1:270506/1‑151 (MQ=255)
                                                         gTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGtt                                                                                       >  1:324006/1‑151 (MQ=255)
                                                           tATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCGCCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGttg                                                                                      <  1:613849/150‑2 (MQ=255)
                                                            aTTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTa                                                                                     <  1:988862/150‑1 (MQ=255)
                                                              tttGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATg                                                                                   <  1:841098/150‑1 (MQ=255)
                                                                 ggTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGa                                                                                  <  1:436331/148‑1 (MQ=255)
                                                                  gtgtGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACgtg                                                                              <  1:1555924/151‑3 (MQ=255)
                                                                      ggATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgaga                                                                          <  1:1500682/151‑1 (MQ=255)
                                                                      ggATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgaga                                                                          <  1:1582687/151‑1 (MQ=255)
                                                                          ggTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTc                                                                       <  2:1710252/150‑1 (MQ=255)
                                                                           gTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTccc                                                                     >  1:643783/1‑151 (MQ=255)
                                                                            tAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTcccc                                                                    >  2:961650/1‑151 (MQ=255)
                                                                              aGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGt                                                                  <  2:338716/151‑1 (MQ=255)
                                                                              aGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGt                                                                  <  2:233395/151‑1 (MQ=255)
                                                                               gCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTc                                                                 <  2:366522/151‑1 (MQ=255)
                                                                               gCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTc                                                                 <  2:661034/151‑1 (MQ=255)
                                                                                        aaaaaTCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAt                                                        >  2:1424881/1‑151 (MQ=255)
                                                                                          aaaTCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAttt                                                      <  1:8886/151‑1 (MQ=255)
                                                                                          aaaTCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAttt                                                      >  1:778376/1‑151 (MQ=255)
                                                                                                atcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTaa                                                <  2:1496993/151‑1 (MQ=255)
                                                                                                atcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTa                                                 >  1:619889/1‑150 (MQ=255)
                                                                                                   aaaCCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCGCTTGAGCGACAGAGAGGAGTGGGGCATTATCGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAAttt                                             <  2:1662780/151‑1 (MQ=255)
                                                                                                     accaccACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGa                                           <  2:733954/151‑1 (MQ=255)
                                                                                                       caccacAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAat                                         >  1:551634/1‑151 (MQ=255)
                                                                                                         ccacAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAat                                         >  2:463002/1‑149 (MQ=255)
                                                                                                            caAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCg                                     >  2:389459/1‑150 (MQ=255)
                                                                                                            caAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGa                                    <  1:563715/151‑1 (MQ=255)
                                                                                                            caAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGa                                    <  2:846550/151‑1 (MQ=255)
                                                                                                             aaaGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGAc                                   <  1:394195/151‑1 (MQ=255)
                                                                                                             aaaGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGAc                                   <  1:719508/151‑1 (MQ=255)
                                                                                                               aGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACg                                  >  2:1320401/1‑150 (MQ=255)
                                                                                                                gATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGtt                                <  1:871229/151‑1 (MQ=255)
                                                                                                                  tGTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTtat                              >  2:1114160/1‑151 (MQ=255)
                                                                                                                   gTTATTTATGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTtata                             <  1:104735/151‑1 (MQ=255)
                                                                                                                    ttatttatGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTatat                            >  1:1563871/1‑151 (MQ=255)
                                                                                                                    ttatttatGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGCCGGTTGACATTTACTTAATTTGAATATCGACGTTatat                            >  2:32165/1‑151 (MQ=255)
                                                                                                                     tatttatGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTatat                            <  1:201930/150‑1 (MQ=255)
                                                                                                                      atttatGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAtt                           <  2:1072404/150‑1 (MQ=255)
                                                                                                                        ttatGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAttttt                        >  2:613629/1‑151 (MQ=255)
                                                                                                                         tatGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGCCGTTATAtttttt                       >  2:1608/1‑151 (MQ=255)
                                                                                                                         tatGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAtttttt                       >  2:1442760/1‑151 (MQ=255)
                                                                                                                          atGTGTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTa                      <  1:167736/151‑1 (MQ=255)
                                                                                                                           tgtgTCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAtttttt                       >  2:415354/1‑149 (MQ=255)
                                                                                                                            gtgtCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAg                     <  2:1580125/150‑1 (MQ=255)
                                                                                                                             tgtCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgtt                   >  1:551583/1‑151 (MQ=255)
                                                                                                                              gtCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgtt                   <  1:691612/150‑1 (MQ=255)
                                                                                                                               tCGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgtttt                 >  2:878467/1‑151 (MQ=255)
                                                                                                                                cGTGAGTGATGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttg                <  1:1057675/151‑1 (MQ=255)
                                                                                                                                 gtgagtgaTGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgt               <  1:1383732/151‑1 (MQ=255)
                                                                                                                                 gtgagtgaTGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgt               <  1:1303653/151‑1 (MQ=255)
                                                                                                                                   gagtgaTGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgttt             >  1:1373278/1‑151 (MQ=255)
                                                                                                                                   gagtgaTGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgttt             <  1:1205325/151‑1 (MQ=255)
                                                                                                                                   gagtgaTGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgttt             >  2:946135/1‑151 (MQ=255)
                                                                                                                                   gagtgaTGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgttt             <  1:561900/151‑1 (MQ=255)
                                                                                                                                    agtgaTGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgtttt            >  2:162332/1‑151 (MQ=255)
                                                                                                                                        aTGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACg        >  2:477192/1‑151 (MQ=255)
                                                                                                                                         tGTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACgg       >  1:711064/1‑151 (MQ=255)
                                                                                                                                          gTCTTGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACgg       <  1:1579010/150‑1 (MQ=255)
                                                                                                                                              tGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGcgtc  >  1:1107350/1‑151 (MQ=255)
                                                                                                                                              tGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGcgt   <  2:828543/150‑1 (MQ=255)
                                                                                                                                              tGTTTGCATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGcgt   <  1:961577/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTC  >  NC_000913/1226832‑1227124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: