Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,254,563 T→C T464A (ACA→GCA)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,254,5630TC99.0% 349.8 / ‑3.9 95T464A (ACA→GCA) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (41/53);  total (42/53)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATGTTGCGTTGCGATGGCCATCTGGTCGGGTG  >  NC_000913/1254421‑1254709
                                                                                                                                              |                                                                                                                                                  
gACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCg                                                                                                                                            >  1:636689/1‑151 (MQ=255)
    cGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGAccc                                                                                                                                        <  1:661245/151‑1 (MQ=255)
     gTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCa                                                                                                                                       <  2:1143678/151‑1 (MQ=255)
       ggctggcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAgg                                                                                                                                     <  2:1541974/151‑1 (MQ=255)
          tggcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTCCTTGTTACGTTCCAGACGCTTTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGTCCCAggg                                                                                                                                    <  1:1283523/149‑1 (MQ=255)
          tggcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTg                                                                                                                                  >  1:957258/1‑151 (MQ=255)
           ggcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGt                                                                                                                                   <  2:1575077/149‑1 (MQ=255)
           ggcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTg                                                                                                                                  <  2:652117/150‑1 (MQ=255)
            gcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCa                                                                                                                                <  1:1172891/151‑1 (MQ=255)
             cAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCat                                                                                                                               <  1:1600440/151‑1 (MQ=255)
               aaaTCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCatat                                                                                                                             >  1:260932/1‑151 (MQ=255)
                 aTCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATcc                                                                                                                           >  2:1308777/1‑151 (MQ=255)
                 aTCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATcc                                                                                                                           <  2:1618615/151‑1 (MQ=255)
                   cGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAg                                                                                                                         <  1:528895/151‑1 (MQ=255)
                   cGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAg                                                                                                                         <  1:1319937/151‑1 (MQ=255)
                      cAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAgatt                                                                                                                      >  1:533517/1‑151 (MQ=255)
                       aGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAgattg                                                                                                                     >  1:756044/1‑151 (MQ=255)
                       aGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAgatt                                                                                                                      <  1:1345464/150‑1 (MQ=255)
                         ttGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAgattgat                                                                                                                   >  2:823398/1‑151 (MQ=255)
                         ttGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAgattga                                                                                                                    <  2:1407600/150‑1 (MQ=255)
                           gAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAgattgatt                                                                                                                  <  2:721109/150‑1 (MQ=255)
                            aaTGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAgattgatt                                                                                                                  >  1:1510315/1‑149 (MQ=255)
                            aaTGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAg                                                                                                                <  1:1654030/151‑1 (MQ=255)
                             aTGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGc                                                                                                               >  1:1040937/1‑151 (MQ=255)
                              tGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGcc                                                                                                              >  1:1691618/1‑151 (MQ=255)
                                  tCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCAcc                                                                                                          >  2:986563/1‑151 (MQ=255)
                                    aGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCtt                                                                                                        >  2:198035/1‑151 (MQ=255)
                                     gcgcCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCttt                                                                                                       <  1:481151/151‑1 (MQ=255)
                                     gcgcCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCttt                                                                                                       <  2:1453137/151‑1 (MQ=255)
                                     gcgcCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCttt                                                                                                       <  2:754057/151‑1 (MQ=255)
                                          aGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGaaa                                                                                                  <  2:450505/151‑1 (MQ=255)
                                          aGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGaaa                                                                                                  <  2:1198324/151‑1 (MQ=255)
                                            cGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGa                                                                                                <  2:608686/151‑1 (MQ=255)
                                            cGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGa                                                                                                <  2:514042/151‑1 (MQ=255)
                                            cGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGa                                                                                                <  2:1589528/151‑1 (MQ=255)
                                               gtCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAAc                                                                                              <  2:601212/150‑1 (MQ=255)
                                               gtCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAAc                                                                                              <  1:718814/150‑1 (MQ=255)
                                               gtCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGAGTGATTAGCCCACCTTTGGAAAGAACg                                                                                             >  2:1580883/1‑151 (MQ=255)
                                                tCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGc                                                                                            >  2:179023/1‑151 (MQ=255)
                                                 cGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCt                                                                                           <  1:1293588/151‑1 (MQ=255)
                                                   agaTTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTAc                                                                                         <  2:1391679/151‑1 (MQ=255)
                                                    gaTTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTAcc                                                                                        <  1:309845/151‑1 (MQ=255)
                                                     aTTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCg                                                                                       >  2:468199/1‑151 (MQ=255)
                                                        aGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGtt                                                                                     <  1:15529/150‑1 (MQ=255)
                                                          aaTTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGAt                                                                                  >  2:814590/1‑151 (MQ=255)
                                                          aaTTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGAt                                                                                  <  1:334615/151‑1 (MQ=255)
                                                           aTTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGAt                                                                                  >  1:1159533/1‑150 (MQ=255)
                                                           aTTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGAt                                                                                  >  2:1493381/1‑150 (MQ=255)
                                                            ttACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATa                                                                                 >  2:1424673/1‑150 (MQ=255)
                                                            ttACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATa                                                                                 >  2:333575/1‑150 (MQ=255)
                                                             tACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATa                                                                                 >  2:1240476/1‑149 (MQ=255)
                                                              aCTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAg                                                                               <  1:594483/150‑1 (MQ=255)
                                                                  gTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATa                                                                           <  1:553574/150‑1 (MQ=255)
                                                                    tACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACg                                                                         >  1:160332/1‑150 (MQ=255)
                                                                    tACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACg                                                                         >  1:20417/1‑150 (MQ=255)
                                                                       gTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGAccc                                                                     <  1:144129/151‑1 (MQ=255)
                                                                         tCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCg                                                                    >  1:414371/1‑150 (MQ=255)
                                                                         tCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCg                                                                    <  2:72390/150‑1 (MQ=255)
                                                                            aGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGc                                                                <  1:1294427/151‑1 (MQ=255)
                                                                            aGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGc                                                                >  2:1622058/1‑151 (MQ=255)
                                                                               cGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGtt                                                             <  2:902688/151‑1 (MQ=255)
                                                                                 cTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTg                                                            >  1:1709581/1‑150 (MQ=255)
                                                                                  tGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGAt                                                          >  2:129258/1‑151 (MQ=255)
                                                                                   gTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGAt                                                          <  1:407216/150‑1 (MQ=255)
                                                                                   gTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATg                                                         >  2:900500/1‑151 (MQ=255)
                                                                                    ttATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGc                                                        >  2:402237/1‑151 (MQ=255)
                                                                                         cATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTg                                                   <  2:1416409/151‑1 (MQ=255)
                                                                                          aTTGAAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTgg                                                  >  1:1473167/1‑151 (MQ=255)
                                                                                               aaCGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTggcggc                                              >  2:891183/1‑150 (MQ=255)
                                                                                               aaCGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAGCCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCa                                             <  1:217833/151‑1 (MQ=255)
                                                                                               aaCGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCa                                             <  1:1378304/151‑1 (MQ=255)
                                                                                                 cGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCAt                                            >  2:570350/1‑150 (MQ=255)
                                                                                                    ccAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAAcc                                        <  1:1678126/151‑1 (MQ=255)
                                                                                                     cAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCa                                       <  2:842640/151‑1 (MQ=255)
                                                                                                       gTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATc                                     >  2:148777/1‑151 (MQ=255)
                                                                                                        ttttCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAACCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCa                                       >  2:973143/1‑148 (MQ=255)
                                                                                                         tttCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCg                                    <  2:1395799/150‑1 (MQ=255)
                                                                                                          ttCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTc                                  >  1:1528287/1‑151 (MQ=255)
                                                                                                           tCCGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTc                                  <  2:267634/150‑1 (MQ=255)
                                                                                                            ccGCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCa                                 <  2:453636/150‑1 (MQ=255)
                                                                                                              gCCATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATgt                              >  2:428227/1‑151 (MQ=255)
                                                                                                               ccATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATgtt                             <  1:1192367/151‑1 (MQ=255)
                                                                                                                cATTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATgtt                             >  1:1655835/1‑150 (MQ=255)
                                                                                                                 aTTGACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATgttgc                           >  2:1564707/1‑151 (MQ=255)
                                                                                                                    gACATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATgttgcgtt                        <  2:453814/151‑1 (MQ=255)
                                                                                                                     aCATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATgttgcgttg                       <  1:268451/151‑1 (MQ=255)
                                                                                                                     aCATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATgttgcgttg                       >  1:1579423/1‑151 (MQ=255)
                                                                                                                     aCATTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATgttgcgttg                       <  1:1435528/151‑1 (MQ=255)
                                                                                                                       aTTATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATGttgcgttgcg                     >  2:415871/1‑151 (MQ=255)
                                                                                                                         tATCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATGTTGCGTTGCGAt                   >  1:908396/1‑151 (MQ=255)
                                                                                                                          aTCGCTGAGGGAGGAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATGTTGCGTTGCGATg                  <  2:414403/151‑1 (MQ=255)
                                                                                                                             gCTGAGGGAGGAACCTGCCCAAACCGACCTAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATGTTGCGTTGCGATGGcc               >  2:296410/1‑151 (MQ=255)
                                                                                                                                gagggaggAACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATGTTGCGTTGCGATGGCCATc            <  2:106483/151‑1 (MQ=255)
                                                                                                                                   ggaggaACCTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATGTTGCGTTGCGATGGCCATCTgg         <  1:570299/151‑1 (MQ=255)
                                                                                                                                          ccTGCCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATGTTGCGTTGCGATGGCCATCTGGTCGGgtg  <  1:1240379/151‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCATACGACCCGAGGCGTTGATGCGGCTGGCGGCATAACCATCGTCATGTTGCGTTGCGATGGCCATCTGGTCGGGTG  >  NC_000913/1254421‑1254709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑