Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,539,343 T→C E1083G (GAA→GGA)  narZ ← nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,539,3430TC99.0% 383.7 / ‑4.1 103E1083G (GAA→GGA) narZnitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (50/52);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01

Only 100 of 103 total aligned reads displayed.

TATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCG  >  NC_000913/1539197‑1539485
                                                                                                                                                  |                                                                                                                                              
tATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCgg                                                                                                                                            >  1:1549978/1‑151 (MQ=255)
 aTCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCgg                                                                                                                                            <  1:1276614/150‑1 (MQ=255)
  tCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCggg                                                                                                                                           >  2:43796/1‑150 (MQ=255)
   cGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGa                                                                                                                                          >  2:228564/1‑150 (MQ=255)
      cAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGcc                                                                                                                                      >  1:800044/1‑151 (MQ=255)
      cAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGcc                                                                                                                                      >  2:923795/1‑151 (MQ=255)
           gTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGAAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTg                                                                                                                                 <  2:1530995/151‑1 (MQ=255)
              aGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCg                                                                                                                              <  2:1277665/151‑1 (MQ=255)
              aGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCg                                                                                                                              >  2:527987/1‑151 (MQ=255)
              aGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCg                                                                                                                              >  1:741356/1‑151 (MQ=255)
               gTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCgg                                                                                                                             <  2:627217/151‑1 (MQ=255)
                 tctcGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGt                                                                                                                            <  1:127085/150‑1 (MQ=255)
                   tcGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATc                                                                                                                         >  2:671057/1‑151 (MQ=255)
                    cGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCt                                                                                                                        <  1:1244886/151‑1 (MQ=255)
                       gCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGgc                                                                                                                     >  1:927412/1‑151 (MQ=255)
                         aTCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGgcg                                                                                                                    >  1:792343/1‑150 (MQ=255)
                           cTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCAt                                                                                                                 >  2:666681/1‑151 (MQ=255)
                            tGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGCGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCAtc                                                                                                                >  2:963646/1‑151 (MQ=255)
                             gTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCAt                                                                                                                 <  1:1240674/149‑1 (MQ=255)
                              tttCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCAtctc                                                                                                              >  2:1468587/1‑151 (MQ=255)
                                   cTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTg                                                                                                          >  1:1053627/1‑150 (MQ=255)
                                   cTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTg                                                                                                          >  2:1459563/1‑150 (MQ=255)
                                    tGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGAc                                                                                                        >  2:153658/1‑151 (MQ=255)
                                     gATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGAc                                                                                                        <  2:408046/150‑1 (MQ=255)
                                        ccAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCt                                                                                                     >  2:1702815/1‑150 (MQ=255)
                                        ccAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCt                                                                                                     <  1:1565632/150‑1 (MQ=255)
                                        ccAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCt                                                                                                     <  1:1357121/150‑1 (MQ=255)
                                        ccAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTa                                                                                                    <  2:1550074/151‑1 (MQ=255)
                                          aGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACg                                                                                                  >  2:609028/1‑151 (MQ=255)
                                            aCAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACggg                                                                                                <  2:861690/151‑1 (MQ=255)
                                              aaTCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGgtg                                                                                              <  2:1696744/151‑1 (MQ=255)
                                              aaTCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGgtg                                                                                              <  1:814106/151‑1 (MQ=255)
                                               aTCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGgtgt                                                                                             >  1:333044/1‑151 (MQ=255)
                                                 cGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTc                                                                                            <  1:219647/150‑1 (MQ=255)
                                                 cGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCg                                                                                           <  1:1377778/151‑1 (MQ=255)
                                                    tCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGAt                                                                                         >  2:37920/1‑150 (MQ=255)
                                                      cACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAgg                                                                                      >  2:1590005/1‑151 (MQ=255)
                                                       aCCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAggc                                                                                     >  1:1659695/1‑151 (MQ=255)
                                                        ccGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAggcg                                                                                    >  1:404872/1‑151 (MQ=255)
                                                            gagagaGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAggcgggcg                                                                                >  1:1387051/1‑151 (MQ=255)
                                                             agagagCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGa                                                                               <  1:340730/151‑1 (MQ=255)
                                                                gagCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATa                                                                             >  1:210049/1‑150 (MQ=255)
                                                                 agCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAg                                                                           <  1:1562228/151‑1 (MQ=255)
                                                                 agCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAg                                                                           <  2:944181/151‑1 (MQ=255)
                                                                 agCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAg                                                                           <  2:1395287/151‑1 (MQ=255)
                                                                  gCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGc                                                                          >  1:826061/1‑151 (MQ=255)
                                                                   cGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGcc                                                                         <  1:1685247/151‑1 (MQ=255)
                                                                    gTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGcca                                                                        <  2:230386/151‑1 (MQ=255)
                                                                      cAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGccacc                                                                      >  1:927556/1‑151 (MQ=255)
                                                                       aGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGccacc                                                                      >  2:907737/1‑150 (MQ=255)
                                                                        gCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTCCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGccacca                                                                     <  1:756119/150‑1 (MQ=255)
                                                                         cATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGCAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGGGATAAGCCACCAgg                                                                   >  1:1241455/1‑151 (MQ=255)
                                                                            tAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCTGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCtt                                                                >  1:292452/1‑151 (MQ=255)
                                                                             aGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCttt                                                               <  2:1218834/151‑1 (MQ=255)
                                                                             aGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCtt                                                                <  1:787964/150‑1 (MQ=255)
                                                                               cAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCttt                                                               >  1:1314833/1‑149 (MQ=255)
                                                                               cAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCa                                                             <  2:293872/151‑1 (MQ=255)
                                                                                aGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCAc                                                            >  2:463101/1‑151 (MQ=255)
                                                                                  gTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCa                                                          <  1:552678/151‑1 (MQ=255)
                                                                                   ttttCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCa                                                          >  2:1292533/1‑150 (MQ=255)
                                                                                     ttCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCaaa                                                        >  1:946138/1‑150 (MQ=255)
                                                                                       cACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGc                                                     <  1:809710/151‑1 (MQ=255)
                                                                                         cTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCAc                                                   >  2:614686/1‑151 (MQ=255)
                                                                                           gTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCAc                                                   <  2:1269638/149‑1 (MQ=255)
                                                                                            tAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGc                                                 >  1:1233645/1‑150 (MQ=255)
                                                                                            tAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGc                                                 >  1:1520486/1‑150 (MQ=255)
                                                                                             aGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCAt                                               <  1:1574951/151‑1 (MQ=255)
                                                                                             aGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCAt                                               <  2:391001/151‑1 (MQ=255)
                                                                                                ttGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATcc                                             >  2:1065365/1‑150 (MQ=255)
                                                                                                ttGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATcc                                             >  2:1396987/1‑150 (MQ=255)
                                                                                                  gAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCAc                                           <  1:923728/150‑1 (MQ=255)
                                                                                                  gAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACg                                          <  2:1691467/151‑1 (MQ=255)
                                                                                                  gAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACg                                          >  1:804134/1‑151 (MQ=255)
                                                                                                  gAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACg                                          <  1:803753/151‑1 (MQ=255)
                                                                                                   aGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACgg                                         <  1:738560/151‑1 (MQ=255)
                                                                                                      gAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGgatg                                      >  1:1527482/1‑151 (MQ=255)
                                                                                                       aaTGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCTGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGgatg                                      <  1:1383687/150‑1 (MQ=255)
                                                                                                         tGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGgatg                                      <  2:501119/148‑1 (MQ=255)
                                                                                                          gCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATcc                                  >  2:187671/1‑151 (MQ=255)
                                                                                                            cccATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTg                                >  1:750156/1‑151 (MQ=255)
                                                                                                             ccATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGa                               <  2:566727/151‑1 (MQ=255)
                                                                                                              cATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGa                               <  2:448081/150‑1 (MQ=255)
                                                                                                              cATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGAt                              >  1:1175904/1‑151 (MQ=255)
                                                                                                              cATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGAt                              >  1:1248453/1‑151 (MQ=255)
                                                                                                               aTTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCGCGCATCCACGGATGATCCTGATa                             <  2:469428/151‑1 (MQ=255)
                                                                                                               aTTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGAt                              >  2:1605428/1‑150 (MQ=255)
                                                                                                                  tCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATaga                           <  2:938513/150‑1 (MQ=255)
                                                                                                                    tGGTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAgc                         >  1:509388/1‑150 (MQ=255)
                                                                                                                     ggTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATagag                          <  2:1395556/148‑1 (MQ=255)
                                                                                                                     ggTGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAgctg                       <  2:927479/151‑1 (MQ=255)
                                                                                                                       tGCGGCGTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAgctgct                     >  2:386268/1‑151 (MQ=255)
                                                                                                                         cggcgTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTgg                   <  1:734969/151‑1 (MQ=255)
                                                                                                                          ggcgTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGc                  <  1:1175210/151‑1 (MQ=255)
                                                                                                                             gTCAGGAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTc               >  1:1345709/1‑151 (MQ=255)
                                                                                                                                  gAAGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGAc          >  2:1076055/1‑151 (MQ=255)
                                                                                                                                    aGTTAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAg        <  1:478911/151‑1 (MQ=255)
                                                                                                                                       tAAGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCg      <  1:666622/150‑1 (MQ=255)
                                                                                                                                        aaGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTg    <  1:1257379/151‑1 (MQ=255)
                                                                                                                                         aGTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTgc   <  1:892719/151‑1 (MQ=255)
                                                                                                                                          gTGCTTTTCCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTgcg  <  1:305929/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
TATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCG  >  NC_000913/1539197‑1539485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: