Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,863,313	T→C	A181A (GCT→GCC)	gapA →	glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,863,313	0	T	C	100.0%	326.2 / NA	86	A181A (GCT→GCC)	gapA	glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/38);  total (48/38)

ACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGG  >  NC_000913/1863174‑1863458                                                                                                                                            |                                                                                                                                                  aCTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAAc                                                                                                                                        >  1:680521/1‑151 (MQ=255)   ttCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCg                                                                                                                                      >  2:747962/1‑151 (MQ=255)   ttCGAAAAATATGCGGCCCAGAACTTCTTTCCCACCGCTTCCTGCACCCCCAACTGCTTGCCTCCGCTGGCTAAAGTTATCAACGATATCTGCGGCATCATCGAAGGTCTGATGCCCCCCTTTCCCGCTACTACCGCCACTCAGaaaa                                                                                                                                         <  1:502345/148‑1 (MQ=255)    tCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCCCCGTTCACGCTACTACCGCCACTCAGAAAACCg                                                                                                                                      <  2:89467/150‑1 (MQ=255)         aaaTTTGCTGCCCAGGACATCGTTTCCACCGCTTCCTGCACCCCCAACTCCCTGGCTCCGCTGGCAAAAGTTACCAACGATAACTTCGGCATCACCGAAGGTCTGATGCCCCCCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGAt                                                                                                                                 <  2:666456/150‑1 (MQ=255)         aaaTATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATg                                                                                                                                >  2:1559561/1‑151 (MQ=255)            tatGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGcc                                                                                                                             >  1:54570/1‑151 (MQ=255)              tGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCg                                                                                                                           >  1:1396968/1‑151 (MQ=255)                cgggCAAGGCCATGGTTTCCAACGCTTCCGGAACCACCAACGGCCTGGCTCCGCTGGCTAAAGTTAAAACCGATACCTTCGGCATCATCGAAGTTCTGATGCCCCCCGTCCACGCTACGACCGCCACTCAGAAAACCGTTGATGGCCCGt                                                                                                                          <  1:1480270/148‑1 (MQ=255)                cTGGCCAGGACATCGTTTCCAACGCTTCCTGCCCCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCCCCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCg                                                                                                                           <  2:1445018/149‑1 (MQ=255)                    ccAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTcac                                                                                                                     >  2:806437/1‑151 (MQ=255)                    ccAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTcac                                                                                                                     >  2:323098/1‑151 (MQ=255)                      aggaattCGTTACCAACGCTCCATACACCCCCACCTGCGTGGTTCTGCGGCCAAAATTTCCCAAAGAAATTTTCGGAATAATGGAGGGTCTGATGACCCCCGTTCGCCCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGtctc                                                                                                                       <  1:695709/141‑1 (MQ=255)                       ggACATCGTTCCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCGGGCTAAAGTTATCAACAATAATTTCGGCATAACGGAAGTTCTGATGACCACCTTGCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACaaa                                                                                                                  <  2:141522/151‑1 (MQ=255)                          cATCTTTCCCACCCCTCCCTGCCCCCCCACCTGCCGGGCTCCGCGGGCTAAATTTATCAACAAAAACTTCGGAATCATCAAGGGTCTGATGCCCCCCTTCCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAg                                                                                                                 <  1:958103/149‑1 (MQ=255)                          cATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGAc                                                                                                               >  2:424404/1‑151 (MQ=255)                           aTCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACt                                                                                                              >  2:1221669/1‑151 (MQ=255)                            tCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTg                                                                                                             >  1:831218/1‑151 (MQ=255)                             cGTTTCAAACTCTTCCTACCCCCCCAACCGCTGGGGCCGGCTGGAAAAAGTTAAAAACGATAACTTGGCCGACACTGAAGTTCTGAGTCCCACCTTCCAGCCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTgg                                                                                                            <  2:234715/151‑1 (MQ=255)                              gTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGgc                                                                                                           >  2:1099278/1‑151 (MQ=255)                               tttCCAACGCTTCCTGCCCCCCCAACTGCCTGGCTCCGCTGGCTAAAGTTATAAACGATAACTTCGGCATCATCGAAGGTCTGATGACCCCCTTTCCCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTg                                                                                                             <  2:1160316/148‑1 (MQ=255)                               ttcccACCGCTTCCTGCCCCCCCACCTGCCTGGCTCCGCTGGTTAAATTTATCAACGATAACTCCGGCATCATCGAGGGTCTGATGCCCCCCGTTCACGCTACTCCCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTgg                                                                                                            <  2:1193474/146‑1 (MQ=255)                                ttCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGgcgc                                                                                                         >  2:1086609/1‑151 (MQ=255)                                  ccAACGCTTCCTGCACCCCCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCCCCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGcgcg                                                                                                        <  2:1594479/150‑1 (MQ=255)                                      cGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCggcggc                                                                                                   >  1:804940/1‑151 (MQ=255)                                      cGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCggcggc                                                                                                   >  1:1440305/1‑151 (MQ=255)                                       gCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGcc                                                                                                  >  1:55229/1‑151 (MQ=255)                                         ttCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCcg                                                                                                 >  1:978715/1‑150 (MQ=255)                                           ccTGCCCCCCCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTCCGGCATCATCGAAGGTCTGATGACCCCCGTTCCCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCcg                                                                                                 <  2:1082853/148‑1 (MQ=255)                                           ccTGCCCCACCAACTGCCTGGCCCCGCTGCCTAAATTTACCAACAATAACTCCGGCATCAGCGAAGGTCTGATGCCCCCCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGcc                                                                                                  <  1:1035685/147‑1 (MQ=255)                                           ccTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCgcgg                                                                                              >  1:1437800/1‑151 (MQ=255)                                            cTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGcctctgc                                                                                             >  2:272730/1‑146 (MQ=255)                                            cTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCgcggc                                                                                             >  1:1204356/1‑151 (MQ=255)                                               cccccccaCCTCCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTCCGGCATCATCGAAGGTCTGATGCCCCCCGTTCCCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCt                                                                                          <  2:28005/146‑1 (MQ=255)                                               caccccCAACTGCCGGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAGGGTCTGATGACCCCCGTTCACGCTCCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCt                                                                                          <  1:1421955/151‑1 (MQ=255)                                                  caccaACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTcc                                                                                       >  2:699689/1‑151 (MQ=255)                                                   accaACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTccc                                                                                      >  2:54949/1‑151 (MQ=255)                                                           ccTGGCTCCGCTGCTTAAAGTTACCAACGATAACTGCGGCATCATCGAAGGTCTGATCACCACCGTTCCCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAAc                                                                                 <  2:99481/148‑1 (MQ=255)                                                              gcccccGCTGGCTAAATTTACCAACAATAACTCCGGCATCATCAAAGGTCTGATGCCCCCCTTCCACGCTCCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAAcatcat                                                                            <  1:1559466/146‑1 (MQ=255)                                                                ccccGCTGGCTAAATTTATCAACGATACCTCCGGCAACATCGAGGGTCTGATGCCCCCCGTCCCCGCTCCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATccc                                                                         <  1:222893/149‑1 (MQ=255)                                                                cTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATccc                                                                         >  2:1112102/1‑151 (MQ=255)                                                                 tCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATcc                                                                          >  2:1641720/1‑149 (MQ=255)                                                                 tCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCg                                                                        >  1:1128828/1‑151 (MQ=255)                                                                  ccGCTGGCTAAAGTTATCACCGATAACTTCGGCATCATCGAAGGTCTGATGCCCACCGTTCACGCTCCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGt                                                                       <  1:298353/151‑1 (MQ=255)                                                                      tGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCctc                                                                   >  1:1464433/1‑151 (MQ=255)                                                                        gCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCctct                                                                  >  1:724644/1‑150 (MQ=255)                                                                           aaaGTTACCACCGTTACCTGCGGCTCCTGCCAAGGTATGATGGCCCCCTTTCGCTATACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCg                                                              <  1:1578035/151‑1 (MQ=255)                                                                            aaGTTATCAACGAAACCTTCGGCATCATCGAAGGTCTGATGCCCCCCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCgg                                                             <  2:685701/151‑1 (MQ=255)                                                                             aGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCgg                                                             >  1:1697364/1‑150 (MQ=255)                                                                             aGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCgg                                                             >  1:579122/1‑150 (MQ=255)                                                                                    aaCGATACCTTCGGCATCATCGAAGGTCTGATGCCCCCCGTTCACGCTATTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTgctgct                                                      <  2:17478/150‑1 (MQ=255)                                                                                    aaCGATAACTTCGGCATCATCGAAGGTCTGATGCCCCCCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGtgct                                                         <  1:459008/147‑1 (MQ=255)                                                                                     aCGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTa                                                     >  2:1327961/1‑150 (MQ=255)                                                                                      cGATAACTTCGGCATCATCGAAGGTCTGATGACCCCCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTaaa                                                   <  1:185528/151‑1 (MQ=255)                                                                                        aTACCTCCGGCATCATCGAAGGTCTGATGCCCCCCGTTCACGCTCCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTaa                                                    <  1:1201356/148‑1 (MQ=255)                                                                                        aTAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGc                                                 >  2:202282/1‑151 (MQ=255)                                                                                        aTAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGc                                                 <  2:689882/151‑1 (MQ=255)                                                                                          aaCTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGTCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGGGCTGCTAAAGCTg                                               >  2:489507/1‑151 (MQ=255)                                                                                          aaCTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTg                                               >  1:1102993/1‑151 (MQ=255)                                                                                              tCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAgg                                           >  2:1222300/1‑151 (MQ=255)                                                                                                  catcatCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTaa                                        >  2:1632407/1‑150 (MQ=255)                                                                                                     catcGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGt                                     >  1:1257522/1‑150 (MQ=255)                                                                                                      atcGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTAc                                   >  2:1609547/1‑151 (MQ=255)                                                                                                       tcGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACt                                  >  1:1557942/1‑151 (MQ=255)                                                                                                       tcGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACt                                  >  1:491843/1‑151 (MQ=255)                                                                                                         gAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTg                                 >  2:173326/1‑150 (MQ=255)                                                                                                         gAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGc                                >  1:1195314/1‑151 (MQ=255)                                                                                                            ggTCTGATGACCCCCTTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCa                              <  2:582528/150‑1 (MQ=255)                                                                                                             gTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGcc                               >  2:1212483/1‑148 (MQ=255)                                                                                                                tgatgaCCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACt                         >  2:44812/1‑151 (MQ=255)                                                                                                                 gatgcCCCCCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACt                         <  1:340775/150‑1 (MQ=255)                                                                                                                 gatgaCCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTg                        >  2:481000/1‑151 (MQ=255)                                                                                                                   tgaCCACCGTTCACCCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGaa                           <  1:327276/146‑1 (MQ=255)                                                                                                                    gcccgccttccACGCTTCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCCTCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGa                            <  1:267589/134‑1 (MQ=255)                                                                                                                      cccccGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATg                    <  1:169784/147‑1 (MQ=255)                                                                                                                      ccaccGTCCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAAt                     <  1:721209/149‑1 (MQ=255)                                                                                                                       caccGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGc                  >  1:1449693/1‑151 (MQ=255)                                                                                                                          agTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCaa                >  2:955078/2‑150 (MQ=255)                                                                                                                            tccccGCTACTCCCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAac              <  2:748476/146‑1 (MQ=255)                                                                                                                              cccGCTCCTACCGCCACTCAGAAATCCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAact             <  2:278528/147‑1 (MQ=255)                                                                                                                               aCGCTTTTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCaa                <  2:173418/145‑1 (MQ=255)                                                                                                                               aCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgac          >  1:701892/1‑151 (MQ=255)                                                                                                                                 gCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCAGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgact         <  2:1182764/150‑1 (MQ=255)                                                                                                                                     ctacCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTa     >  1:540072/1‑150 (MQ=255)                                                                                                                                      tacCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATg   >  2:1010991/1‑151 (MQ=255)                                                                                                                                        ccGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATgg  <  2:1697480/150‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                  ACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGG  >  NC_000913/1863174‑1863458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑