Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,865,673 A→G intergenic (‑37/+53) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,6730AG97.4% 427.8 / 0.6 114intergenic (‑37/+53)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (56/55);  total (56/58)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 118 total aligned reads displayed.

AGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGC  >  NC_000913/1865524‑1865820
                                                                                                                                                     |                                                                                                                                                   
aGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAAc                                                                                                                                                    <  1:117655/151‑1 (MQ=255)
    cagcaACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAggg                                                                                                                                                >  1:1121144/1‑151 (MQ=255)
     agcaACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGc                                                                                                                                               <  1:477385/151‑1 (MQ=255)
         aCTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTg                                                                                                                                            >  1:985048/1‑150 (MQ=255)
         aCTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTg                                                                                                                                            >  1:1677818/1‑150 (MQ=255)
         aCTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGa                                                                                                                                           <  2:1355133/151‑1 (MQ=255)
         aCTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGAACCACCGTAATGCAAAGCAGGGCGTg                                                                                                                                            >  1:1356483/1‑150 (MQ=255)
            tCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACg                                                                                                                                         <  1:689076/150‑1 (MQ=255)
                ttttGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCt                                                                                                                                     <  1:1526527/150‑1 (MQ=255)
                  ttGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGtt                                                                                                                                  >  1:454987/1‑151 (MQ=255)
                       cTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTAt                                                                                                                             >  2:280798/1‑151 (MQ=255)
                        tGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATg                                                                                                                            >  1:1374384/1‑151 (MQ=255)
                        tGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATg                                                                                                                            >  1:366668/1‑151 (MQ=255)
                         gACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGc                                                                                                                           >  1:8556/1‑151 (MQ=255)
                          aCTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGca                                                                                                                          <  2:1416072/151‑1 (MQ=255)
                          aCTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGc                                                                                                                           >  2:1451144/1‑150 (MQ=255)
                             ggCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGcaca                                                                                                                        <  1:1451046/150‑1 (MQ=255)
                               cATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACaaa                                                                                                                      >  2:1577335/1‑150 (MQ=255)
                                  cTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGc                                                                                                                  >  2:354395/1‑151 (MQ=255)
                                   ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                 >  2:125814/1‑151 (MQ=255)
                                   ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                 >  1:675079/1‑151 (MQ=255)
                                    tCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAAt                                                                                                                    <  2:1226663/147‑1 (MQ=255)
                                    tCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAAt                                                                                                                    <  2:1506954/147‑1 (MQ=255)
                                    tCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATg                                                                                                                   <  2:906987/148‑1 (MQ=255)
                                    tCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGccc                                                                                                                >  2:1361609/1‑151 (MQ=255)
                                     cGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGccc                                                                                                                <  2:394157/150‑1 (MQ=255)
                                     cGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGGGACGCCCTGTTTTTATGCACAAAATGCCCt                                                                                                               <  2:1592345/151‑1 (MQ=255)
                                       cccATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTgg                                                                                                             >  1:14713/1‑151 (MQ=255)
                                        ccATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTgg                                                                                                             >  2:1175202/1‑150 (MQ=255)
                                         cATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGaa                                                                                                           <  1:1028373/151‑1 (MQ=255)
                                          atatACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAACTTGAATCATCTTTTGCTGCATTGTTTCCTCCCAGGTATTGCACCACCGTCATGCAAAGCAGGGCGTGACGCCCTGCTTTTATGCCCAAAATGCCCTgg                                                                                                             >  1:61670/1‑148 (MQ=255)
                                          atatACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGaaa                                                                                                          <  2:758880/151‑1 (MQ=255)
                                          atatACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGaaa                                                                                                          <  2:1121230/151‑1 (MQ=255)
                                          atatACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGaa                                                                                                           >  2:562160/1‑150 (MQ=255)
                                            ataCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGa                                                                                                        <  2:1440488/151‑1 (MQ=255)
                                              aCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                                                       <  1:326686/150‑1 (MQ=255)
                                              aCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                                                       <  1:1014891/150‑1 (MQ=255)
                                                cATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCa                                                                                                    <  2:285607/151‑1 (MQ=255)
                                                   gTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTa                                                                                                 <  2:1179320/151‑1 (MQ=255)
                                                    ttCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTa                                                                                                 <  1:1622245/150‑1 (MQ=255)
                                                     tCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTAt                                                                                                <  1:348168/150‑1 (MQ=255)
                                                     tCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATc                                                                                               >  2:1376784/1‑151 (MQ=255)
                                                       ccTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATc                                                                                               >  2:93027/1‑149 (MQ=255)
                                                       ccTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATc                                                                                               >  2:107638/1‑149 (MQ=255)
                                                           cccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtt                                                                                         <  1:933270/151‑1 (MQ=255)
                                                           cccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTACATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtt                                                                                         >  2:1511934/1‑151 (MQ=255)
                                                            ccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAttt                                                                                        <  1:1099057/151‑1 (MQ=255)
                                                             cTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTg                                                                                       >  2:463475/1‑151 (MQ=255)
                                                             cTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTg                                                                                       >  2:970693/1‑151 (MQ=255)
                                                               aCGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTg                                                                                       <  1:612261/149‑1 (MQ=255)
                                                                cGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGt                                                                                      >  1:169454/1‑149 (MQ=255)
                                                                  gCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                                                                  >  1:527067/1‑151 (MQ=255)
                                                                   cTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTg                                                                                 >  2:483465/1‑151 (MQ=255)
                                                                     ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTg                                                                                 <  2:295407/149‑1 (MQ=255)
                                                                     ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGa                                                                                <  2:602343/150‑1 (MQ=255)
                                                                     ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGa                                                                                <  2:670411/150‑1 (MQ=255)
                                                                     ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAt                                                                               <  2:1698577/151‑1 (MQ=255)
                                                                     ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAt                                                                               <  1:1691604/151‑1 (MQ=255)
                                                                      gCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATc                                                                              <  1:1613599/151‑1 (MQ=255)
                                                                       cAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAt                                                                               <  2:347813/149‑1 (MQ=255)
                                                                         gTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCg                                                                           >  2:1440062/1‑151 (MQ=255)
                                                                           gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGt                                                                         >  2:1179197/1‑151 (MQ=255)
                                                                               cATCGCCTCTAAATTGAATCATGTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGa                                                                      <  2:1053566/150‑1 (MQ=255)
                                                                               cATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaa                                                                     <  2:1488646/151‑1 (MQ=255)
                                                                               cATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGa                                                                      >  1:581201/1‑150 (MQ=255)
                                                                                aTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaaa                                                                    <  2:298517/151‑1 (MQ=255)
                                                                                  cGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATc                                                                  <  1:1224540/151‑1 (MQ=255)
                                                                                    ccACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATc                                                                  >  2:1663166/1‑149 (MQ=255)
                                                                                      aCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                              <  2:1368586/151‑1 (MQ=255)
                                                                                          aaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                                                          >  2:1259653/1‑151 (MQ=255)
                                                                                              gAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                                                        >  2:689909/1‑149 (MQ=255)
                                                                                                  cATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                  >  2:574878/1‑151 (MQ=255)
                                                                                                      ttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTAt                                               <  2:464232/150‑1 (MQ=255)
                                                                                                        ttgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcc                                             >  1:88592/1‑150 (MQ=255)
                                                                                                         tgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccac                                           >  1:1282144/1‑151 (MQ=255)
                                                                                                         tgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccac                                           >  2:62631/1‑151 (MQ=255)
                                                                                                          gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacc                                          >  1:488916/1‑151 (MQ=255)
                                                                                                           ttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacc                                          >  1:201538/1‑150 (MQ=255)
                                                                                                             gCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATc                                       >  2:259793/1‑151 (MQ=255)
                                                                                                              cATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCg                                      <  1:633230/151‑1 (MQ=255)
                                                                                                              cATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCg                                      >  1:1356041/1‑151 (MQ=255)
                                                                                                               aTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCgg                                     <  2:1668339/151‑1 (MQ=255)
                                                                                                                    ttCCTCCCAGGAATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGCTAGAAATCAGGCCAGTCCAGGATTTATCCACCATc                                       >  2:1400884/1‑144 (MQ=255)
                                                                                                                      cctccCAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTc                               <  1:1227472/150‑1 (MQ=255)
                                                                                                                         cccAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGt                            <  1:759008/150‑1 (MQ=255)
                                                                                                                          ccAGGTATTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTa                           >  2:908364/1‑150 (MQ=255)
                                                                                                                                aTTGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtca                     <  2:1458501/150‑1 (MQ=255)
                                                                                                                                 ttGCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGCAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcatc                   >  1:250501/1‑151 (MQ=255)
                                                                                                                                   gCACCACCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcag                 <  1:614602/151‑1 (MQ=255)
                                                                                                                                    caccacCGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcatc                   >  2:719605/1‑148 (MQ=255)
                                                                                                                                         ccGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga            <  1:1034600/150‑1 (MQ=255)
                                                                                                                                         ccGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga            <  2:985125/150‑1 (MQ=255)
                                                                                                                                          cGTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACg          <  1:1533167/151‑1 (MQ=255)
                                                                                                                                           gTAATGCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACgg         <  1:280775/151‑1 (MQ=255)
                                                                                                                                                gCAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGgtgt     <  2:366696/150‑1 (MQ=255)
                                                                                                                                                 cAAAGCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAg   <  1:60253/151‑1 (MQ=255)
                                                                                                                                                  aaagCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTTTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGc  >  2:114184/1‑151 (MQ=255)
                                                                                                                                                  aaagCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGc  <  1:1675140/151‑1 (MQ=255)
                                                                                                                                                  aaagCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGc  >  1:140277/1‑151 (MQ=255)
                                                                                                                                                  aaaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGc  >  2:413623/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGC  >  NC_000913/1865524‑1865820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: