Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,967,290 C→T G50S (GGT→AGT)  cheY ← chemotaxis regulator transmitting signal to flagellar motor component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,967,2900CT98.1% 399.8 / ‑2.2 105G50S (GGT→AGT) cheYchemotaxis regulator transmitting signal to flagellar motor component
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (51/52);  total (52/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 105 total aligned reads displayed.

GCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATCCGCCAT  >  NC_000913/1967142‑1967437
                                                                                                                                                    |                                                                                                                                                   
gccgcAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgc                                                                                                                                                   <  1:1048587/151‑1 (MQ=255)
  cgcAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgcct                                                                                                                                                 >  1:1100891/1‑151 (MQ=255)
  cgcAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgcc                                                                                                                                                  >  2:1135518/1‑150 (MQ=255)
   gcagcaATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgcctg                                                                                                                                                >  1:997333/1‑151 (MQ=255)
      gcaATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgcctgcct                                                                                                                                             >  2:889785/1‑151 (MQ=255)
      gcaATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgcctgcct                                                                                                                                             >  1:1080611/1‑151 (MQ=255)
       caATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGcctgcctg                                                                                                                                            >  2:732855/1‑151 (MQ=255)
              gTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACtt                                                                                                                                      >  1:1252049/1‑150 (MQ=255)
               ttCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTAt                                                                                                                                    >  2:746261/1‑151 (MQ=255)
                    tttcttCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTg                                                                                                                                  >  1:61690/1‑148 (MQ=255)
                     ttcttcGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTgag                                                                                                                                <  2:718072/149‑1 (MQ=255)
                        ttcGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGt                                                                                                                           >  2:739350/1‑151 (MQ=255)
                           gCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgcc                                                                                                                                                  <  2:1380374/125‑1 (MQ=255)
                           gCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgcc                                                                                                                                                  >  1:1380280/1‑125 (MQ=255)
                             ttCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACg                                                                                                                      >  2:310412/1‑151 (MQ=255)
                                tGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGcc                                                                                                                    >  2:202600/1‑150 (MQ=255)
                                 gCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCa                                                                                                                   <  1:1410760/150‑1 (MQ=255)
                                  cAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGc                                                                                                                     <  2:1062960/147‑1 (MQ=255)
                                      cACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTcc                                                                                                             >  2:845890/1‑151 (MQ=255)
                                      cACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTcc                                                                                                             >  1:63546/1‑151 (MQ=255)
                                      cACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTcc                                                                                                             <  2:1222838/151‑1 (MQ=255)
                                       aCCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCg                                                                                                            >  2:731969/1‑151 (MQ=255)
                                          aTTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCtt                                                                                                         <  2:1385815/151‑1 (MQ=255)
                                          aTTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCtt                                                                                                         >  2:1624931/1‑151 (MQ=255)
                                          aTTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCtt                                                                                                         >  1:1219418/1‑151 (MQ=255)
                                              acacTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTc                                                                                                     >  1:203907/1‑151 (MQ=255)
                                              acacTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTc                                                                                                     >  2:1356183/1‑151 (MQ=255)
                                                acTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCa                                                                                                    >  1:1594552/1‑150 (MQ=255)
                                                 cTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAAc                                                                                                  >  2:1653856/1‑151 (MQ=255)
                                                   ggCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACat                                                                                                <  2:425744/151‑1 (MQ=255)
                                                     cAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACatt                                                                                               >  1:1314239/1‑150 (MQ=255)
                                                      aaTGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACattat                                                                                             >  1:1068004/1‑151 (MQ=255)
                                                      aaTGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACattat                                                                                             <  1:494776/151‑1 (MQ=255)
                                                       aTGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACattatt                                                                                            >  1:544019/1‑151 (MQ=255)
                                                       aTGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACattatt                                                                                            <  2:170175/151‑1 (MQ=255)
                                                       aTGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACattatt                                                                                            >  2:386677/1‑151 (MQ=255)
                                                          ccGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGaa                                                                                         <  2:1291975/151‑1 (MQ=255)
                                                           cGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAAt                                                                                        <  1:507697/151‑1 (MQ=255)
                                                           cGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAAt                                                                                        <  2:1252146/151‑1 (MQ=255)
                                                            gACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATc                                                                                       >  2:513700/1‑151 (MQ=255)
                                                            gACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATc                                                                                       <  1:455054/151‑1 (MQ=255)
                                                            gACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATc                                                                                       >  2:361592/1‑151 (MQ=255)
                                                             aCATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATcc                                                                                      >  1:1172123/1‑151 (MQ=255)
                                                              cATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATccc                                                                                     >  2:78967/1‑151 (MQ=255)
                                                               aTCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATccc                                                                                     >  1:693470/1‑150 (MQ=255)
                                                               aTCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCa                                                                                    <  1:429568/151‑1 (MQ=255)
                                                                  gcgcCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGct                                                                                 <  2:589638/151‑1 (MQ=255)
                                                                    gcCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGctct                                                                               >  1:247043/1‑151 (MQ=255)
                                                                    gcCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGctct                                                                               <  2:362427/151‑1 (MQ=255)
                                                                      cATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCttt                                                                             >  2:90975/1‑151 (MQ=255)
                                                                       aTCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTc                                                                            <  2:1270411/151‑1 (MQ=255)
                                                                            cACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTcagc                                                                         >  2:651058/1‑149 (MQ=255)
                                                                            cACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTcagc                                                                         >  2:260284/1‑149 (MQ=255)
                                                                             aCGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAgg                                                                      <  1:716678/151‑1 (MQ=255)
                                                                                   tGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGc                                                                >  2:1007139/1‑151 (MQ=255)
                                                                                   tGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGc                                                                >  2:518231/1‑151 (MQ=255)
                                                                                          aGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATg                                                          <  1:961745/150‑1 (MQ=255)
                                                                                          aGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGc                                                         <  2:853930/151‑1 (MQ=255)
                                                                                          aGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGc                                                         <  2:350442/151‑1 (MQ=255)
                                                                                           gCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCg                                                        <  2:1553914/151‑1 (MQ=255)
                                                                                             aaTTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTc                                                      <  1:1007882/151‑1 (MQ=255)
                                                                                             aaTTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTc                                                      <  2:1049826/151‑1 (MQ=255)
                                                                                             aaTTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTc                                                      >  1:327340/1‑151 (MQ=255)
                                                                                              aTTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCg                                                     >  2:378080/1‑151 (MQ=255)
                                                                                                  cAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCAtg                                                 <  2:1717060/151‑1 (MQ=255)
                                                                                                  cAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCAtg                                                 >  1:1375630/1‑151 (MQ=255)
                                                                                                   aGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCAtgg                                                >  2:914173/1‑151 (MQ=255)
                                                                                                    ggCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCAtggt                                               <  2:223178/151‑1 (MQ=255)
                                                                                                      ccatccatATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCAtggtgg                                             <  1:1621025/151‑1 (MQ=255)
                                                                                                       catccatATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCAtggtgg                                             >  2:895162/1‑150 (MQ=255)
                                                                                                       catccatATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGa                                            <  2:61694/151‑1 (MQ=255)
                                                                                                        atccatATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGa                                            >  2:1419132/1‑150 (MQ=255)
                                                                                                          ccatATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGaaaa                                         <  1:895094/151‑1 (MQ=255)
                                                                                                           catATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAg                                        <  1:538416/151‑1 (MQ=255)
                                                                                                           catATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAg                                        <  2:800692/151‑1 (MQ=255)
                                                                                                               ttGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCAt                                    >  2:1431206/1‑151 (MQ=255)
                                                                                                                tGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCAt                                    <  1:672130/150‑1 (MQ=255)
                                                                                                                   gCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCcac                                <  1:1634845/151‑1 (MQ=255)
                                                                                                                     aTGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCcaca                               >  2:1655420/1‑150 (MQ=255)
                                                                                                                     aTGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACaa                              <  2:72414/151‑1 (MQ=255)
                                                                                                                     aTGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACaa                              <  2:1063179/151‑1 (MQ=255)
                                                                                                                      tGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAAc                             >  1:1035098/1‑151 (MQ=255)
                                                                                                                       gTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAAcc                            >  2:743835/1‑151 (MQ=255)
                                                                                                                       gTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAAc                             <  2:1579891/150‑1 (MQ=255)
                                                                                                                         tCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCa                           <  2:247065/150‑1 (MQ=255)
                                                                                                                          ccAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCaa                          >  2:211697/1‑150 (MQ=255)
                                                                                                                             gTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAAt                      <  1:472367/151‑1 (MQ=255)
                                                                                                                              tCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAAtt                     >  2:964299/1‑151 (MQ=255)
                                                                                                                               cGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAAttt                    <  1:141589/151‑1 (MQ=255)
                                                                                                                               cGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAAtt                     <  1:409077/150‑1 (MQ=255)
                                                                                                                                 gagaTAACAAACCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTaa                  <  1:1135430/151‑1 (MQ=255)
                                                                                                                                   gaTAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGt                <  2:646214/151‑1 (MQ=255)
                                                                                                                                       aCAATTCCATAGCCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCtt            <  2:1172210/151‑1 (MQ=255)
                                                                                                                                       aCAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCtt            <  2:1314334/151‑1 (MQ=255)
                                                                                                                                       aCAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCtt            >  2:111144/1‑151 (MQ=255)
                                                                                                                                         aaaTCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCttt           >  1:489328/1‑150 (MQ=255)
                                                                                                                                             ccATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATcc       <  2:203924/150‑1 (MQ=255)
                                                                                                                                             ccATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATcc       <  1:34076/150‑1 (MQ=255)
                                                                                                                                               aTAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATCCGcc    <  1:889716/151‑1 (MQ=255)
                                                                                                                                                 aaCTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATCCGCCAt  <  1:513648/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATCCGCCAT  >  NC_000913/1967142‑1967437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: