Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,974,562 T→C T255A (ACG→GCG)  cheA ← fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,974,5620TC99.1% 412.2 / ‑3.9 114T255A (ACG→GCG) cheAfused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (59/54);  total (59/55)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 118 total aligned reads displayed.

ATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCAT  >  NC_000913/1974414‑1974708
                                                                                                                                                    |                                                                                                                                                  
aTCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGctt                                                                                                                                                  <  2:356961/151‑1 (MQ=255)
  cAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGTGCGTCGctttt                                                                                                                                                <  2:704527/151‑1 (MQ=255)
    aaTCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTc                                                                                                                                              >  2:787634/1‑151 (MQ=255)
      tCACCATGATTAACCGGGTCCAGTTCGCCGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTccc                                                                                                                                            >  1:680223/1‑151 (MQ=255)
           aTGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTcc                                                                                                                                       >  2:997073/1‑151 (MQ=255)
             gATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCa                                                                                                                                      <  1:1048324/150‑1 (MQ=255)
             gATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAc                                                                                                                                     >  2:947956/1‑151 (MQ=255)
             gATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAc                                                                                                                                     >  2:1359069/1‑151 (MQ=255)
                tAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAcgcg                                                                                                                                  >  2:1234280/1‑151 (MQ=255)
                tAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAcgc                                                                                                                                   <  2:1352777/150‑1 (MQ=255)
                   ccGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGcc                                                                                                                               <  1:778043/151‑1 (MQ=255)
                     gggTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCgg                                                                                                                             >  1:1195189/1‑151 (MQ=255)
                       gTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGtt                                                                                                                           <  1:40114/151‑1 (MQ=255)
                         ccAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTg                                                                                                                          <  2:757713/150‑1 (MQ=255)
                          cAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGgc                                                                                                                        >  2:972077/1‑151 (MQ=255)
                              tCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCtt                                                                                                                    >  2:1480006/1‑151 (MQ=255)
                                   ggAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTc                                                                                                                <  1:443215/150‑1 (MQ=255)
                                     aaCGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGc                                                                                                             >  1:1590917/1‑151 (MQ=255)
                                        gCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGc                                                                                                          >  1:552077/1‑151 (MQ=255)
                                        gCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGc                                                                                                          >  1:488663/1‑151 (MQ=255)
                                        gCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGc                                                                                                          >  2:724436/1‑151 (MQ=255)
                                        gCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGc                                                                                                          >  1:1109832/1‑151 (MQ=255)
                                          tGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGcc                                                                                                         >  2:1013413/1‑150 (MQ=255)
                                             gCAAGCATGGACTGGGCGATAACCAGCTCGCCGACGAGGCTAACTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCCCGTCGCTTTTTCCCGCTCCACGCGGCCGGCTGGCGCTTGTTCGGCTGCCAGtt                                                                                                     >  1:445823/1‑151 (MQ=255)
                                               aaGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGttt                                                                                                    >  1:593962/1‑150 (MQ=255)
                                                aGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTa                                                                                                   >  1:739894/1‑150 (MQ=255)
                                                 gCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAg                                                                                                 <  1:320732/151‑1 (MQ=255)
                                                   aTGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCa                                                                                               <  2:1166438/151‑1 (MQ=255)
                                                    tGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCAc                                                                                              >  2:715575/1‑151 (MQ=255)
                                                      gACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtg                                                                                            <  2:154134/151‑1 (MQ=255)
                                                          gggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtggtgg                                                                                        <  1:1142043/151‑1 (MQ=255)
                                                           ggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTggtggg                                                                                       <  1:539361/151‑1 (MQ=255)
                                                              gATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTggtggggtg                                                                                    <  2:508240/151‑1 (MQ=255)
                                                                tAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTggtggggtgg                                                                                   >  2:81338/1‑150 (MQ=255)
                                                                 aaCCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGa                                                                                  <  2:481210/150‑1 (MQ=255)
                                                                  aCCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGata                                                                                <  1:1549946/151‑1 (MQ=255)
                                                                  aCCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGat                                                                                 >  1:733619/1‑150 (MQ=255)
                                                                   ccAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGatat                                                                               <  2:203736/151‑1 (MQ=255)
                                                                    cAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATAtt                                                                              >  1:297144/1‑151 (MQ=255)
                                                                         cGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATAttttt                                                                           <  2:265477/149‑1 (MQ=255)
                                                                         cGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATAttttt                                                                           <  1:1678527/149‑1 (MQ=255)
                                                                          gCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGc                                                                        <  1:1079715/151‑1 (MQ=255)
                                                                            cgacgaGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCg                                                                       >  1:1613292/1‑150 (MQ=255)
                                                                              acgaGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCg                                                                       >  2:1162678/1‑148 (MQ=255)
                                                                               cgaGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGAc                                                                   >  1:375125/1‑151 (MQ=255)
                                                                               cgaGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGAc                                                                   >  2:1690415/1‑151 (MQ=255)
                                                                                   gTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTc                                                                <  2:189508/150‑1 (MQ=255)
                                                                                   gTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCt                                                               >  2:1665947/1‑151 (MQ=255)
                                                                                    ttaattaaTTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCt                                                               >  2:455919/1‑150 (MQ=255)
                                                                                     taattaatTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTa                                                              <  1:726942/150‑1 (MQ=255)
                                                                                     taattaatTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTAc                                                             >  1:1576950/1‑151 (MQ=255)
                                                                                      aattaattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTAc                                                             <  2:873205/150‑1 (MQ=255)
                                                                                      aattaattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACt                                                            <  1:862988/151‑1 (MQ=255)
                                                                                      aattaattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACt                                                            <  2:1195279/151‑1 (MQ=255)
                                                                                       attaattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTg                                                           >  2:1006633/1‑151 (MQ=255)
                                                                                         taattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGGGGCTATTTTTGGCGAGACTTCTACTGtt                                                         >  1:550285/1‑151 (MQ=255)
                                                                                          aattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGtt                                                         <  1:1473065/150‑1 (MQ=255)
                                                                                             tGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTc                                                       <  2:1235249/149‑1 (MQ=255)
                                                                                             tGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCa                                                      >  2:1254928/1‑150 (MQ=255)
                                                                                             tGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCa                                                      >  1:1444572/1‑150 (MQ=255)
                                                                                              gATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCaaa                                                    <  2:934606/151‑1 (MQ=255)
                                                                                              gATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGAGGGGAGGATATTTTTGGCGAGACTTCTACTGTTTCaa                                                     >  2:183901/1‑150 (MQ=255)
                                                                                               aTCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAg                                                   <  2:789237/151‑1 (MQ=255)
                                                                                                 cAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAgg                                                  <  2:1591013/150‑1 (MQ=255)
                                                                                                 cAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAgg                                                  >  1:917876/1‑150 (MQ=255)
                                                                                                   aCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGGGCTTTTTCGGCTGCCAGTTTCAGCACTGGTGGGGAGGATATTTTTGGCGAGACTTCTAGTGTTTCAAAGGTa                                                >  1:543058/1‑150 (MQ=255)
                                                                                                    ccTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAAt                                              <  2:1540230/151‑1 (MQ=255)
                                                                                                    ccTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAAt                                              <  2:641355/151‑1 (MQ=255)
                                                                                                       tttCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCt                                            <  2:1041160/150‑1 (MQ=255)
                                                                                                        ttCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTg                                           >  1:1360331/1‑150 (MQ=255)
                                                                                                         tCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGAt                                         >  2:1379004/1‑151 (MQ=255)
                                                                                                          cTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATc                                        >  1:596492/1‑151 (MQ=255)
                                                                                                            aCCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATc                                        <  2:656618/149‑1 (MQ=255)
                                                                                                            aCCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCgg                                      <  2:732273/151‑1 (MQ=255)
                                                                                                             ccGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCGCGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTGGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGc                                     <  1:228739/151‑1 (MQ=255)
                                                                                                             ccGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGc                                     >  1:1260429/1‑151 (MQ=255)
                                                                                                               gCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCtt                                   >  1:457030/1‑151 (MQ=255)
                                                                                                                 tACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTc                                  >  2:1421333/1‑150 (MQ=255)
                                                                                                                    acGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATc                              >  2:329150/1‑151 (MQ=255)
                                                                                                                      ggATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATcac                            >  1:259650/1‑151 (MQ=255)
                                                                                                                        aTGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACaa                          <  2:704947/151‑1 (MQ=255)
                                                                                                                         tGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACaa                          >  2:1513503/1‑150 (MQ=255)
                                                                                                                          gCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACaaa                         <  2:1650188/150‑1 (MQ=255)
                                                                                                                             ggtggATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACa                      <  2:109760/150‑1 (MQ=255)
                                                                                                                              gtggATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACaga                    <  1:561790/151‑1 (MQ=255)
                                                                                                                              gtggATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACaga                    <  1:1606072/151‑1 (MQ=255)
                                                                                                                                ggATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGt                  >  1:1364288/1‑151 (MQ=255)
                                                                                                                                 gATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGt                  >  1:616336/1‑150 (MQ=255)
                                                                                                                                 gATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTa                 <  2:307626/151‑1 (MQ=255)
                                                                                                                                  aTTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTAc                >  1:1142949/1‑151 (MQ=255)
                                                                                                                                    tCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTAcc               <  2:370673/150‑1 (MQ=255)
                                                                                                                                      aTTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCt            <  2:654192/151‑1 (MQ=255)
                                                                                                                                        tgctgcGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCtgt          >  1:487870/1‑151 (MQ=255)
                                                                                                                                         gctgcGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCtgtg         <  1:1479908/151‑1 (MQ=255)
                                                                                                                                          ctgcGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCtgtg         >  2:1047895/1‑150 (MQ=255)
                                                                                                                                          ctgcGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCtgtg         >  1:341771/1‑150 (MQ=255)
                                                                                                                                          ctgcGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCtgtg         >  2:1367343/1‑150 (MQ=255)
                                                                                                                                            gcgcGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATg      <  2:980090/151‑1 (MQ=255)
                                                                                                                                              gcgTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTc    <  2:963066/151‑1 (MQ=255)
                                                                                                                                                gtcgcTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCAt  <  2:1334611/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCAT  >  NC_000913/1974414‑1974708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: