Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,992,730 A→G W30R (TGG→CGG)  pgsA ← phosphatidylglycerophosphate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,992,7300AG95.2% 361.4 / 4.3 103W30R (TGG→CGG) pgsAphosphatidylglycerophosphate synthetase
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base G (41/57);  total (42/61)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCA  >  NC_000913/1992583‑1992876
                                                                                                                                                   |                                                                                                                                                  
tGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGt                                                                                                                                                 >  2:289804/1‑151 (MQ=255)
    gATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGAc                                                                                                                                              >  2:1323327/1‑150 (MQ=255)
    gATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACa                                                                                                                                             <  1:305808/151‑1 (MQ=255)
     aTAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGAc                                                                                                                                              >  1:1343044/1‑149 (MQ=255)
      tAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAgg                                                                                                                                           >  2:1280439/1‑151 (MQ=255)
      tAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAg                                                                                                                                            <  2:619654/150‑1 (MQ=255)
         ccACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAACCCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCa                                                                                                                                         <  1:611335/150‑1 (MQ=255)
         ccACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCa                                                                                                                                         <  1:535118/150‑1 (MQ=255)
          cACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGc                                                                                                                                          >  1:1552225/1‑148 (MQ=255)
            cGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGAt                                                                                                                                      >  2:971745/1‑150 (MQ=255)
             gagaACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATa                                                                                                                                     >  2:41551/1‑150 (MQ=255)
              agaACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATaa                                                                                                                                    >  2:443648/1‑150 (MQ=255)
              agaACTTTACCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTCCTCTGGTTCCAGCGGCGTCCCAGAAAACCATCGAACAAGTCAGGCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATaa                                                                                                                                    <  1:143209/150‑1 (MQ=255)
               gaACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATaaa                                                                                                                                   >  2:1468675/1‑150 (MQ=255)
                aaCTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATaaa                                                                                                                                   >  1:720222/1‑149 (MQ=255)
                  cTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGAc                                                                                                                               >  2:1456109/1‑151 (MQ=255)
                   tttATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGAcc                                                                                                                              >  2:226822/1‑151 (MQ=255)
                             acaGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACaa                                                                                                                       >  1:1287638/1‑148 (MQ=255)
                               aGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGaa                                                                                                                   <  1:883312/150‑1 (MQ=255)
                                gggTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATg                                                                                                                 <  1:854855/151‑1 (MQ=255)
                                  gTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATggg                                                                                                               <  2:1141243/151‑1 (MQ=255)
                                         aaaGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGa                                                                                                        <  1:1468577/151‑1 (MQ=255)
                                          aaGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAACCCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGCGATAAGGAt                                                                                                       <  1:469842/151‑1 (MQ=255)
                                          aaGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGAt                                                                                                       <  1:1250174/151‑1 (MQ=255)
                                             cACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGa                                                                                                     <  1:1120484/150‑1 (MQ=255)
                                                 aagccGGTTACTCTGGTCCCAGCGGCGTGCCAGAAAACCATCGACCCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACgg                                                                                                <  1:1134837/148‑1 (MQ=255)
                                                  aaCCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACgg                                                                                                >  1:400066/1‑150 (MQ=255)
                                                  aaCCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGa                                                                                               <  1:725177/151‑1 (MQ=255)
                                                  aaCCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGa                                                                                               <  2:333384/151‑1 (MQ=255)
                                                   aCCGGGTACTCTGGTTCCAGCGGCGTGCAAGAAAACCATCGAACCAGCCAGTCTCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGaa                                                                                              <  1:922296/151‑1 (MQ=255)
                                                    ccGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAAc                                                                                             <  2:125180/151‑1 (MQ=255)
                                                      gggTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAAc                                                                                             >  2:769537/1‑149 (MQ=255)
                                                          aCTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAgtgt                                                                                        <  2:1599219/150‑1 (MQ=255)
                                                           ctctGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTa                                                                                       <  2:285461/150‑1 (MQ=255)
                                                             ctGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTaa                                                                                      <  2:336782/149‑1 (MQ=255)
                                                             ctGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTaa                                                                                      <  2:10605/149‑1 (MQ=255)
                                                             ctGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGc                                                                                    >  1:472976/1‑151 (MQ=255)
                                                              tGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGc                                                                                    >  2:1675384/1‑150 (MQ=255)
                                                               ggTTCCAGGGGCGGGGCAGAAAACCATCGACCCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCaa                                                                                  <  2:1623775/151‑1 (MQ=255)
                                                               ggTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGc                                                                                    <  2:1040445/149‑1 (MQ=255)
                                                               ggTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCa                                                                                   <  1:1317993/150‑1 (MQ=255)
                                                               ggTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCa                                                                                   <  1:1605286/150‑1 (MQ=255)
                                                                  tCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGt                                                                               >  1:933693/1‑151 (MQ=255)
                                                                   ccAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATGAGGATGACACGGAACAGTGTAAGCAACGTa                                                                              <  2:228874/151‑1 (MQ=255)
                                                                   ccAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGt                                                                               <  2:1012780/150‑1 (MQ=255)
                                                                    cAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGt                                                                               <  1:1022458/149‑1 (MQ=255)
                                                                    cAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGt                                                                               >  1:558984/1‑149 (MQ=255)
                                                                     aGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAgg                                                                            <  1:510144/151‑1 (MQ=255)
                                                                      gcggcgTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAggg                                                                           <  1:188767/151‑1 (MQ=255)
                                                                       cggcgTGCCAGAAAACCATCGATCCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAggg                                                                           <  1:990107/150‑1 (MQ=255)
                                                                       cggcgTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAggg                                                                           >  2:1137029/1‑150 (MQ=255)
                                                                       cggcgTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAggg                                                                           >  2:1278432/1‑150 (MQ=255)
                                                                           gTGCCAGAAACCCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAgg                                                                            <  1:71510/145‑1 (MQ=255)
                                                                           gTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATAtt                                                                      >  1:1488692/1‑151 (MQ=255)
                                                                           gTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATAtt                                                                      <  1:620441/151‑1 (MQ=255)
                                                                           gTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATAtt                                                                      <  1:41547/151‑1 (MQ=255)
                                                                           gTGCAAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATAtt                                                                      <  1:1173156/151‑1 (MQ=255)
                                                                             gCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTaa                                                                    <  2:1375204/151‑1 (MQ=255)
                                                                             gCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTaa                                                                    <  2:524042/151‑1 (MQ=255)
                                                                                aGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAAtt                                                                 >  2:451353/1‑151 (MQ=255)
                                                                                 gAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTg                                                                >  2:1563009/1‑151 (MQ=255)
                                                                                  aaaaCCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGc                                                               <  2:1201415/151‑1 (MQ=255)
                                                                                    aaCCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCAt                                                             >  1:336566/1‑151 (MQ=255)
                                                                                      ccATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATa                                                            <  2:1669620/150‑1 (MQ=255)
                                                                                        aTCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATg                                                         >  2:738976/1‑151 (MQ=255)
                                                                                        aTCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATg                                                         >  2:209983/1‑151 (MQ=255)
                                                                                          cGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGAc                                                       <  2:264606/151‑1 (MQ=255)
                                                                                           gAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACg                                                      >  1:979561/1‑151 (MQ=255)
                                                                                            aaCCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACgg                                                     <  2:38609/151‑1 (MQ=255)
                                                                                            aaCCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACgg                                                     <  2:519663/151‑1 (MQ=255)
                                                                                              ccAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACggg                                                    <  1:701272/150‑1 (MQ=255)
                                                                                               cagtcagtCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTa                                                  <  1:339919/151‑1 (MQ=255)
                                                                                                agtcagtcACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTaa                                                 <  1:1456011/151‑1 (MQ=255)
                                                                                                   cagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTa                                              <  2:453354/151‑1 (MQ=255)
                                                                                                    agtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTAt                                             >  2:824692/1‑151 (MQ=255)
                                                                                                       caCCGTCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCtg                                          <  2:638131/151‑1 (MQ=255)
                                                                                                       caCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCtg                                          >  2:87602/1‑151 (MQ=255)
                                                                                                          cgccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCtgttg                                       <  2:666536/151‑1 (MQ=255)
                                                                                                            ccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTc                                     >  2:724012/1‑151 (MQ=255)
                                                                                                            ccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTc                                     <  2:88485/151‑1 (MQ=255)
                                                                                                            ccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTc                                     >  1:197140/1‑151 (MQ=255)
                                                                                                             cgcgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTc                                     >  2:913350/1‑150 (MQ=255)
                                                                                                               cgACGCAGAAAATGAGCGCGGCGGCAAACGTCGAAAAGGTGACAAGCAGAAAAAAGACCAGTAGAAAGAATGGGAGAAAGATGAGACGGAACAGAGTAAGCAACGAAGGGATATTAAATTGCATAATGAGGGGTAACTATCtgttgt                                      >  1:770516/1‑147 (MQ=255)
                                                                                                                gACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTa                                 <  1:783989/151‑1 (MQ=255)
                                                                                                                gACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTa                                 <  2:1343137/151‑1 (MQ=255)
                                                                                                                 aCGCAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTaa                                <  1:258149/151‑1 (MQ=255)
                                                                                                                    cAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTa                                 >  2:395726/1‑147 (MQ=255)
                                                                                                                    cAGAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAt                             <  2:279721/151‑1 (MQ=255)
                                                                                                                      gAAAATGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTa                           <  1:245595/151‑1 (MQ=255)
                                                                                                                       aaaaTGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTAc                          <  1:769474/151‑1 (MQ=255)
                                                                                                                         aaTGAGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTAccc                        >  2:1213494/1‑151 (MQ=255)
                                                                                                                             aGCGCGGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTAt                    >  2:3306/1‑151 (MQ=255)
                                                                                                                               cgcgGCGGCAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGt                  >  1:763167/1‑151 (MQ=255)
                                                                                                                                  ggcggcAAACGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGc               <  2:720280/151‑1 (MQ=255)
                                                                                                                                      gcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACa           <  1:1643021/151‑1 (MQ=255)
                                                                                                                                        aaaCGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTAc            >  1:476149/1‑148 (MQ=255)
                                                                                                                                         aaCGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag          >  2:1372920/1‑149 (MQ=255)
                                                                                                                                         aaCGGCGACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag          >  1:1696007/1‑149 (MQ=255)
                                                                                                                                               gACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATc   >  2:1656253/1‑150 (MQ=255)
                                                                                                                                               gACCGGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCa  <  1:1532661/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGGCGATAGCCACGAGAACTTTATCTGCCACAGGGTCAAGGAAAGCACCAAACCGGGTACTCTGGTTCCAGCGGCGTGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCA  >  NC_000913/1992583‑1992876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑