Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,014,519 A→G S431G (AGC→GGC)  fliF → flagellar basal‑body MS‑ring and collar protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,014,5190AG100.0% 461.1 / NA 115S431G (AGC→GGC) fliFflagellar basal‑body MS‑ring and collar protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (56/59);  total (56/59)

Only 100 of 115 total aligned reads displayed.

GGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGCAGCTAACACGTCGC  >  NC_000913/2014374‑2014665
                                                                                                                                                 |                                                                                                                                                  
ggtgAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAg                                                                                                                                                <  1:913564/150‑1 (MQ=255)
ggtgAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGt                                                                                                                                               <  2:1375224/151‑1 (MQ=255)
 gtgAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTg                                                                                                                                              >  1:1058832/1‑151 (MQ=255)
  tgAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTg                                                                                                                                              >  2:582767/1‑150 (MQ=255)
    aaTTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACg                                                                                                                                           <  1:152383/151‑1 (MQ=255)
      ttACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGa                                                                                                                                          >  2:65373/1‑150 (MQ=255)
       tACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGaa                                                                                                                                         <  1:32795/150‑1 (MQ=255)
       tACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGaa                                                                                                                                         >  1:600155/1‑150 (MQ=255)
        aCAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGaaa                                                                                                                                        <  1:748809/150‑1 (MQ=255)
         cAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAgc                                                                                                                                      <  1:1038071/151‑1 (MQ=255)
         cAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAgc                                                                                                                                      >  2:531258/1‑151 (MQ=255)
           aaaCCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAgcgg                                                                                                                                    <  2:84158/151‑1 (MQ=255)
           aaaCCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAgcgg                                                                                                                                    <  1:1338070/151‑1 (MQ=255)
             aCCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAgcggcg                                                                                                                                  <  2:156225/151‑1 (MQ=255)
              ccTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGcggcgg                                                                                                                                 >  1:582458/1‑151 (MQ=255)
               cTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGcggcgg                                                                                                                                 >  2:819315/1‑150 (MQ=255)
                ttGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGcggcgg                                                                                                                                 >  1:1620242/1‑149 (MQ=255)
                  gCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGgaga                                                                                                                              <  2:566416/150‑1 (MQ=255)
                  gCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGaa                                                                                                                             <  2:522904/151‑1 (MQ=255)
                    cAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACt                                                                                                                           <  2:600209/151‑1 (MQ=255)
                      gATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGc                                                                                                                         <  1:219629/151‑1 (MQ=255)
                         ggCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCa                                                                                                                       <  2:253083/150‑1 (MQ=255)
                         ggCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCa                                                                                                                       <  1:167560/150‑1 (MQ=255)
                         ggCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCAt                                                                                                                      >  2:537077/1‑151 (MQ=255)
                          gCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCAtt                                                                                                                     <  2:471667/151‑1 (MQ=255)
                            aaaCCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACTAAAGCGGCGGAGAACTGCCATTCt                                                                                                                   <  1:1661629/151‑1 (MQ=255)
                            aaaCCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCt                                                                                                                   >  1:1234804/1‑151 (MQ=255)
                                cGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGc                                                                                                                >  1:618349/1‑150 (MQ=255)
                                 gTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCaa                                                                                                              <  2:1225725/151‑1 (MQ=255)
                                 gTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCa                                                                                                               >  2:1621474/1‑150 (MQ=255)
                                  ttGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAAc                                                                                                             >  1:1347691/1‑151 (MQ=255)
                                    gCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAg                                                                                                           <  1:967977/151‑1 (MQ=255)
                                      ctctCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGc                                                                                                          >  2:1647082/1‑150 (MQ=255)
                                      ctctCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGc                                                                                                          >  1:1297095/1‑150 (MQ=255)
                                        ctcAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCa                                                                                                         <  2:936681/149‑1 (MQ=255)
                                         tcAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGc                                                                                                      >  2:1413802/1‑151 (MQ=255)
                                         tcAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGc                                                                                                      >  2:1667852/1‑151 (MQ=255)
                                            gCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGtt                                                                                                   <  2:1058327/151‑1 (MQ=255)
                                             cAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGttt                                                                                                  <  1:1105856/151‑1 (MQ=255)
                                             cAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGttt                                                                                                  <  1:1187232/151‑1 (MQ=255)
                                             cAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGttt                                                                                                  <  2:1186090/151‑1 (MQ=255)
                                              aaCGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGttt                                                                                                  >  2:209360/1‑150 (MQ=255)
                                              aaCGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGttt                                                                                                  >  1:1492837/1‑150 (MQ=255)
                                                cGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTAt                                                                                                <  1:1141311/150‑1 (MQ=255)
                                                  aaCAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCg                                                                                              >  1:616369/1‑150 (MQ=255)
                                                         gAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGtt                                                                                       <  1:889534/150‑1 (MQ=255)
                                                          aaGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTAc                                                                                     >  1:1637466/1‑151 (MQ=255)
                                                             cAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACtt                                                                                   <  1:260473/150‑1 (MQ=255)
                                                             cAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtg                                                                                  >  2:1446877/1‑151 (MQ=255)
                                                             cAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtg                                                                                  >  2:1147013/1‑151 (MQ=255)
                                                                aTTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgctg                                                                               >  2:1377513/1‑151 (MQ=255)
                                                                  tGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgctgc                                                                              <  1:1103351/150‑1 (MQ=255)
                                                                  tGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgctgc                                                                              <  1:270833/150‑1 (MQ=255)
                                                                  tGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTtgctgc                                                                              >  1:1095044/1‑150 (MQ=255)
                                                                   gAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCg                                                                            <  1:1482189/151‑1 (MQ=255)
                                                                    aaGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCgg                                                                           <  2:89728/151‑1 (MQ=255)
                                                                     aGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGt                                                                          <  1:1104851/151‑1 (MQ=255)
                                                                      gATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTc                                                                         >  1:1267056/1‑151 (MQ=255)
                                                                           gACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTgg                                                                    >  1:420784/1‑151 (MQ=255)
                                                                           gACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTgg                                                                    >  1:1145220/1‑151 (MQ=255)
                                                                           gACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCAGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTgg                                                                    >  2:1261924/1‑151 (MQ=255)
                                                                             cccGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGtt                                                                  <  1:413689/151‑1 (MQ=255)
                                                                                gcgAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGc                                                                >  2:427537/1‑150 (MQ=255)
                                                                                   aGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGt                                                            >  2:1097132/1‑151 (MQ=255)
                                                                                     gCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAc                                                          >  2:908881/1‑151 (MQ=255)
                                                                                      cGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAct                                                         >  1:201050/1‑151 (MQ=255)
                                                                                      cGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTAct                                                         <  1:1286975/151‑1 (MQ=255)
                                                                                        aTGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTActgc                                                       >  2:694050/1‑151 (MQ=255)
                                                                                          gggCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTActgct                                                      <  1:896201/150‑1 (MQ=255)
                                                                                            gCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCtggt                                                   <  2:1087262/151‑1 (MQ=255)
                                                                                              ttttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCtggtgg                                                 >  2:1092425/1‑151 (MQ=255)
                                                                                              ttttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCtggtgg                                                 >  2:496757/1‑151 (MQ=255)
                                                                                              ttttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCtggtgg                                                 >  1:20373/1‑151 (MQ=255)
                                                                                               tttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCtggtgg                                                 <  2:1658131/150‑1 (MQ=255)
                                                                                               tttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCtggtgg                                                 <  2:1145310/150‑1 (MQ=255)
                                                                                                ttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGgtggcg                                               >  1:419334/1‑151 (MQ=255)
                                                                                                ttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGgtggcg                                               >  1:1085885/1‑151 (MQ=255)
                                                                                                ttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGgtggcg                                               >  1:1259893/1‑151 (MQ=255)
                                                                                                ttCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGgtggc                                                <  1:59489/150‑1 (MQ=255)
                                                                                                    gAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGgtggcgtggc                                           >  2:873513/1‑151 (MQ=255)
                                                                                                     aaaaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGgct                                          >  2:1711403/1‑151 (MQ=255)
                                                                                                     aaaaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGgct                                          <  2:537429/151‑1 (MQ=255)
                                                                                                      aaaaCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGgctg                                         >  1:1407345/1‑151 (MQ=255)
                                                                                                         aCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGctgctg                                      >  1:1531196/1‑151 (MQ=255)
                                                                                                         aCGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGctgctg                                      <  2:182377/151‑1 (MQ=255)
                                                                                                               tGACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCgg                                >  2:1053619/1‑151 (MQ=255)
                                                                                                                gACTCGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGa                               <  2:735365/151‑1 (MQ=255)
                                                                                                                    cGCTCAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGc                           <  2:622261/151‑1 (MQ=255)
                                                                                                                     gCTCAATGTCGCTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCg                          >  2:708400/1‑151 (MQ=255)
                                                                                                                        cAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGt                        >  2:60550/1‑150 (MQ=255)
                                                                                                                        cAATGTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTa                       <  1:722494/151‑1 (MQ=255)
                                                                                                                            gTCGTTAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGt                    <  1:1225030/150‑1 (MQ=255)
                                                                                                                                 tAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGc                >  2:1227402/1‑149 (MQ=255)
                                                                                                                                 tAACTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGCa               <  1:693991/150‑1 (MQ=255)
                                                                                                                                  aaCTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGCAg              <  1:34346/150‑1 (MQ=255)
                                                                                                                                   aCTCGCCGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGCAGCt            <  1:1646759/151‑1 (MQ=255)
                                                                                                                                        ccGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGCAGCTAacac       >  1:1365758/1‑151 (MQ=255)
                                                                                                                                        ccGTTCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGCAGCTAaca        >  1:269973/1‑150 (MQ=255)
                                                                                                                                            tCAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGCAGCTAACACGTc    >  2:1640088/1‑150 (MQ=255)
                                                                                                                                             cAATGGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGCAGCTAACACGTcgc  <  2:1637571/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTCGCCGTTCAATAGCAGTGACGAAAGCGGCGGAGAACTGCCATTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTTGCTGCCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGGAAAGCGGTACGTCCGCAGCTAACACGTCGC  >  NC_000913/2014374‑2014665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: