Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,026,134 C→T T208T (ACC→ACT yedP → putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,026,1340CT98.1% 407.7 / ‑2.2 106T208T (ACC→ACTyedPputative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (51/53);  total (52/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 110 total aligned reads displayed.

CACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGG  >  NC_000913/2025986‑2026277
                                                                                                                                                    |                                                                                                                                               
cacaATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACtac                                                                                                                                               >  1:240603/1‑151 (MQ=255)
 acaATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACtaca                                                                                                                                              <  2:1430598/151‑1 (MQ=255)
   aaTTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACt                                                                                                                                            >  2:240398/1‑151 (MQ=255)
         ccGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTGCACTTGGc                                                                                                                                        <  1:1612981/149‑1 (MQ=255)
         ccGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGcc                                                                                                                                       <  1:1120191/150‑1 (MQ=255)
         ccGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCt                                                                                                                                      <  1:425462/151‑1 (MQ=255)
         ccGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCt                                                                                                                                      >  1:1711652/1‑151 (MQ=255)
          cGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTg                                                                                                                                     <  2:548513/151‑1 (MQ=255)
           gCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTgg                                                                                                                                    >  2:337018/1‑151 (MQ=255)
           gCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTgg                                                                                                                                    <  1:1710120/151‑1 (MQ=255)
            cTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTggg                                                                                                                                   >  1:777384/1‑151 (MQ=255)
             ttgTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGc                                                                                                                                  >  2:1175068/3‑151 (MQ=255)
              cGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGc                                                                                                                                  <  2:1711119/150‑1 (MQ=255)
                 cTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATg                                                                                                                              >  1:1402245/1‑151 (MQ=255)
                   gaacgaacTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATgg                                                                                                                             <  2:1283958/150‑1 (MQ=255)
                      cgaacTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGcc                                                                                                                          >  2:467546/1‑150 (MQ=255)
                      cgaacTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCa                                                                                                                         <  1:1705959/151‑1 (MQ=255)
                       gaacTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCa                                                                                                                         >  1:228107/1‑150 (MQ=255)
                        aacTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCaaa                                                                                                                       <  2:445217/151‑1 (MQ=255)
                        aacTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCaa                                                                                                                        >  2:840577/1‑150 (MQ=255)
                         acTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAAc                                                                                                                      >  2:1043650/1‑151 (MQ=255)
                            gggCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGa                                                                                                                    <  1:893559/150‑1 (MQ=255)
                             gacTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACg                                                                                                                     <  2:1004667/146‑1 (MQ=255)
                               cTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATgcg                                                                                                                >  1:1344069/1‑151 (MQ=255)
                                ttACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATgcgc                                                                                                               >  2:267049/1‑151 (MQ=255)
                                ttACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATgcgc                                                                                                               >  2:143164/1‑151 (MQ=255)
                                 tACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATgcgc                                                                                                               <  1:469927/150‑1 (MQ=255)
                                 tACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATgcgc                                                                                                               <  1:469886/150‑1 (MQ=255)
                                 tACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATgcg                                                                                                                <  1:1602756/149‑1 (MQ=255)
                                 tACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATgca                                                                                                                <  2:307118/149‑2 (MQ=255)
                                 tACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGcc                                                                                                              >  1:711443/1‑151 (MQ=255)
                                  aCAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGccc                                                                                                             >  1:1701450/1‑151 (MQ=255)
                                    aGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCtt                                                                                                           <  2:1568986/151‑1 (MQ=255)
                                     gTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCtt                                                                                                           >  2:610415/1‑150 (MQ=255)
                                      tttATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTAc                                                                                                         >  2:1445083/1‑151 (MQ=255)
                                        tATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACt                                                                                                        <  2:1163724/150‑1 (MQ=255)
                                        tATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACt                                                                                                        <  2:746294/150‑1 (MQ=255)
                                         aTGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTgg                                                                                                      >  2:43813/1‑151 (MQ=255)
                                         aTGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTg                                                                                                       <  1:1020237/150‑1 (MQ=255)
                                          tGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTgg                                                                                                      >  2:1071388/1‑150 (MQ=255)
                                           gCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAg                                                                                                    <  2:1262649/151‑1 (MQ=255)
                                            cAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACTCTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAg                                                                                                    <  2:90944/150‑1 (MQ=255)
                                               ggTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTaa                                                                                                <  2:1665682/151‑1 (MQ=255)
                                                 tGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATg                                                                                              >  2:780628/1‑151 (MQ=255)
                                                  gcgcgcTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATg                                                                                              <  2:710314/150‑1 (MQ=255)
                                                  gcgcgcTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATg                                                                                              <  1:591347/150‑1 (MQ=255)
                                                  gcgcgcTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATg                                                                                              <  1:15353/150‑1 (MQ=255)
                                                    gcgcTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGAt                                                                                           >  1:218884/1‑151 (MQ=255)
                                                    gcgcTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGAt                                                                                           <  1:193500/151‑1 (MQ=255)
                                                      gcTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTa                                                                                         <  1:313205/151‑1 (MQ=255)
                                                       cTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTAc                                                                                        >  2:1586122/1‑151 (MQ=255)
                                                        ttCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTAcg                                                                                       >  2:1585963/1‑151 (MQ=255)
                                                          cTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTAcgcg                                                                                     >  2:33349/1‑151 (MQ=255)
                                                            ggCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTAcgc                                                                                      <  1:1223447/148‑1 (MQ=255)
                                                              cACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTg                                                                                  >  1:1083694/1‑150 (MQ=255)
                                                               aCGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGAt                                                                                <  2:7754/151‑1 (MQ=255)
                                                               aCGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGAt                                                                                <  2:1286532/151‑1 (MQ=255)
                                                                cGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGAtt                                                                               <  1:717865/151‑1 (MQ=255)
                                                                 gTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATtg                                                                              >  2:840187/1‑151 (MQ=255)
                                                                   aCTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATtgt                                                                             <  1:1280205/150‑1 (MQ=255)
                                                                   aCTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATtgt                                                                             <  2:698520/150‑1 (MQ=255)
                                                                     tGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGa                                                                           >  1:1178820/1‑150 (MQ=255)
                                                                        aTGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAgg                                                                       >  1:982219/1‑151 (MQ=255)
                                                                          gCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAggg                                                                      <  2:1425524/150‑1 (MQ=255)
                                                                          gCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGc                                                                     <  2:332107/151‑1 (MQ=255)
                                                                            ttCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCt                                                                    >  2:368419/1‑150 (MQ=255)
                                                                                gCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAAt                                                                <  2:910010/150‑1 (MQ=255)
                                                                                gCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAAt                                                                <  1:699186/150‑1 (MQ=255)
                                                                                gCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATc                                                               >  1:751389/1‑151 (MQ=255)
                                                                                 ccGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCg                                                              <  1:305495/151‑1 (MQ=255)
                                                                                          aTCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGt                                                     <  2:1261085/151‑1 (MQ=255)
                                                                                           tCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTg                                                    >  2:1487070/1‑151 (MQ=255)
                                                                                           tCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTg                                                    >  2:1246632/1‑151 (MQ=255)
                                                                                            cAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTg                                                    <  2:968288/150‑1 (MQ=255)
                                                                                            cAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGc                                                   >  1:1589479/1‑151 (MQ=255)
                                                                                                cTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGTGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCt                                               >  1:834737/1‑151 (MQ=255)
                                                                                                 tGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGGAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGGGCATATg                                              >  1:319251/1‑151 (MQ=255)
                                                                                                    cAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCat                                           >  2:956869/1‑151 (MQ=255)
                                                                                                    cAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCat                                           >  2:976473/1‑151 (MQ=255)
                                                                                                    cAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCat                                           >  1:67928/1‑151 (MQ=255)
                                                                                                        tGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCatgatg                                       >  2:157414/1‑151 (MQ=255)
                                                                                                          gATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCAtgatga                                      <  2:1686463/150‑1 (MQ=255)
                                                                                                           aTTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAgg                                    >  2:804323/1‑151 (MQ=255)
                                                                                                             tATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCCAAATCGTGAAGGGGTGCATCTGCATGATGAGGAt                                  >  1:1034839/1‑151 (MQ=255)
                                                                                                                cgcgACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATcc                                <  1:1367318/150‑1 (MQ=255)
                                                                                                                cgcgACCTATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCg                               >  1:1219775/1‑151 (MQ=255)
                                                                                                                       tATCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCcgcg                        >  2:473193/1‑151 (MQ=255)
                                                                                                                        aTCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCcgc                         <  2:74138/149‑1 (MQ=255)
                                                                                                                        aTCAACAATTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGt                       <  1:1691738/151‑1 (MQ=255)
                                                                                                                          caacaaTTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTc                      <  1:1047874/150‑1 (MQ=255)
                                                                                                                            acaaTTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTgg                   >  2:1613620/1‑151 (MQ=255)
                                                                                                                            acaaTTGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTgg                   >  1:416369/1‑151 (MQ=255)
                                                                                                                                ttGTCAGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGa                >  1:1562008/1‑150 (MQ=255)
                                                                                                                                     aGGCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAg          <  1:240376/151‑1 (MQ=255)
                                                                                                                                       gCAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCg        <  2:1169892/151‑1 (MQ=255)
                                                                                                                                        cAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGt       <  1:1486971/151‑1 (MQ=255)
                                                                                                                                        cAAACGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGt       >  1:1025319/1‑151 (MQ=255)
                                                                                                                                         aaaCGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTg      <  1:1043571/151‑1 (MQ=255)
                                                                                                                                          aaCGCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTg      >  1:639541/1‑150 (MQ=255)
                                                                                                                                             gCCCAACTACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAgg  <  1:780564/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGG  >  NC_000913/2025986‑2026277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: