Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,213,249 A→G F466L (TTT→CTT)  yehU ← inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,213,2490AG95.4% 376.6 / 5.7 109F466L (TTT→CTT) yehUinner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base G (49/55);  total (54/55)
Fisher's exact test for biased strand distribution p-value = 2.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-02

Only 100 of 111 total aligned reads displayed.

GGTTACCGGTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAACGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAA  >  NC_000913/2213100‑2213396
                                                                                                                                                     |                                                                                                                                                   
ggTTACCGGTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTaaa                                                                                                                                                     <  2:1094657/150‑1 (MQ=255)
    aCCGGTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCgg                                                                                                                                                <  2:516192/151‑1 (MQ=255)
    aCCGGTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCgg                                                                                                                                                <  2:1316131/151‑1 (MQ=255)
    aCCGGTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCgg                                                                                                                                                >  1:201577/1‑151 (MQ=255)
     ccGGTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCggg                                                                                                                                               <  1:920567/151‑1 (MQ=255)
     ccGGTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCggg                                                                                                                                               <  1:1635752/151‑1 (MQ=255)
       ggTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGc                                                                                                                                              >  2:566718/1‑150 (MQ=255)
       ggTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCa                                                                                                                                             <  2:411342/151‑1 (MQ=255)
        gatGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCa                                                                                                                                             >  1:1575334/3‑150 (MQ=255)
        gTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCaa                                                                                                                                            <  2:100546/151‑1 (MQ=255)
         ttGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAAt                                                                                                                                           >  1:1564453/1‑151 (MQ=255)
           gATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAAtt                                                                                                                                          <  2:821103/150‑1 (MQ=255)
           gATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAAtt                                                                                                                                          <  1:1485227/150‑1 (MQ=255)
           gATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATtg                                                                                                                                         <  2:1022493/151‑1 (MQ=255)
            aTACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATtgc                                                                                                                                        <  2:319556/151‑1 (MQ=255)
              aCAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATtgctg                                                                                                                                      >  1:697087/1‑151 (MQ=255)
                 aaCCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGctgctg                                                                                                                                   <  2:611440/151‑1 (MQ=255)
                 aaCCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGctgctg                                                                                                                                   >  2:842643/1‑151 (MQ=255)
                  aCCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTgg                                                                                                                                  <  1:572723/151‑1 (MQ=255)
                       cATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATa                                                                                                                              >  1:123571/1‑150 (MQ=255)
                        aTTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAAt                                                                                                                            >  2:1332270/1‑151 (MQ=255)
                        aTTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAAt                                                                                                                            >  1:1495137/1‑151 (MQ=255)
                          tGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAAtt                                                                                                                           <  2:355506/150‑1 (MQ=255)
                          tGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTc                                                                                                                          >  2:970702/1‑151 (MQ=255)
                             cTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCtt                                                                                                                        >  1:1440582/1‑150 (MQ=255)
                              ttCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGc                                                                                                                      >  2:1408103/1‑151 (MQ=255)
                              ttCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGc                                                                                                                      >  2:1178266/1‑151 (MQ=255)
                               tCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCtt                                                                                                                        <  2:1568602/148‑1 (MQ=255)
                               tCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCtt                                                                                                                        <  2:677207/148‑1 (MQ=255)
                                  aTCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGa                                                                                                                   >  1:454841/1‑150 (MQ=255)
                                  aTCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATATCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGa                                                                                                                   >  2:513421/1‑150 (MQ=255)
                                   tctcCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGaa                                                                                                                  >  1:1323855/1‑150 (MQ=255)
                                   tctcCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAAt                                                                                                                 >  1:1154285/1‑151 (MQ=255)
                                      ccAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGc                                                                                                              <  2:518011/151‑1 (MQ=255)
                                      ccAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGc                                                                                                              >  2:199508/1‑151 (MQ=255)
                                       cAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCa                                                                                                             <  1:224852/151‑1 (MQ=255)
                                        aGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCaa                                                                                                            <  2:1411004/151‑1 (MQ=255)
                                          cATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATg                                                                                                          <  2:1282920/151‑1 (MQ=255)
                                             cAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTg                                                                                                       <  2:230946/151‑1 (MQ=255)
                                              aaaTGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTg                                                                                                       >  2:214029/1‑150 (MQ=255)
                                               aaTGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGAc                                                                                                     <  2:215335/151‑1 (MQ=255)
                                                aTGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGAcc                                                                                                    >  1:343176/1‑151 (MQ=255)
                                                 tGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAACGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCt                                                                                                   >  1:1068745/1‑151 (MQ=255)
                                                 tGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAACGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCt                                                                                                   >  2:352869/1‑151 (MQ=255)
                                                  gTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCt                                                                                                   <  1:1020138/150‑1 (MQ=255)
                                                       cccTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGTGGAATAGCAATGTTGACCTGCAAcc                                                                                             >  1:1420459/1‑151 (MQ=255)
                                                        ccTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACcg                                                                                            <  2:1342227/151‑1 (MQ=255)
                                                         cTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACcgc                                                                                           >  1:89367/1‑151 (MQ=255)
                                                           cACGTCGGGCGCTGATTGCCCCTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGa                                                                                         <  1:390361/151‑1 (MQ=255)
                                                           cACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACcgcg                                                                                          >  1:1669558/1‑150 (MQ=255)
                                                           cACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACcgcg                                                                                          <  2:1495238/150‑1 (MQ=255)
                                                              gTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTg                                                                                      <  1:1175646/151‑1 (MQ=255)
                                                              gTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTg                                                                                      <  1:1401365/151‑1 (MQ=255)
                                                                 gggCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGaa                                                                                   <  1:356109/151‑1 (MQ=255)
                                                                 gggCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGaa                                                                                   <  2:1080666/151‑1 (MQ=255)
                                                                    cgcTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAgcg                                                                                >  1:272846/1‑151 (MQ=255)
                                                                         aTTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCt                                                                           <  2:177622/151‑1 (MQ=255)
                                                                           tGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCttt                                                                         >  2:26435/1‑151 (MQ=255)
                                                                            gCCCCTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAGCCGCGACTGGAAGCGCGCCtt                                                                          <  2:812516/149‑1 (MQ=255)
                                                                                  cGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAAt                                                                    <  1:1568871/149‑1 (MQ=255)
                                                                                  cGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGAGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAAttt                                                                  <  2:631493/151‑1 (MQ=255)
                                                                                   gCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTg                                                                 >  2:1449639/1‑151 (MQ=255)
                                                                                     ccTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCa                                                               <  2:527398/151‑1 (MQ=255)
                                                                                        gTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGc                                                                >  1:1005958/1‑147 (MQ=255)
                                                                                         tATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGAt                                                            >  1:1007543/1‑150 (MQ=255)
                                                                                          aTCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGTGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATa                                                           <  2:1420553/150‑1 (MQ=255)
                                                                                          aTCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATaa                                                          <  2:316846/151‑1 (MQ=255)
                                                                                          aTCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATa                                                           >  1:138172/1‑150 (MQ=255)
                                                                                            ccAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAg                                                         <  1:176170/150‑1 (MQ=255)
                                                                                              agcagTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAACGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCAt                                                      >  2:29266/1‑151 (MQ=255)
                                                                                                 agTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTc                                                    >  2:147504/1‑150 (MQ=255)
                                                                                                   ttGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTcac                                                  >  2:729287/1‑150 (MQ=255)
                                                                                                       gATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGtt                                             >  2:872390/1‑151 (MQ=255)
                                                                                                        aTGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTc                                            <  1:492650/151‑1 (MQ=255)
                                                                                                             ccATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAAttt                                       <  2:964499/151‑1 (MQ=255)
                                                                                                              cATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTtc                                      >  2:990796/1‑151 (MQ=255)
                                                                                                               aTGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTtcg                                     >  2:41490/1‑151 (MQ=255)
                                                                                                               aTGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTtcg                                     >  1:1221194/1‑151 (MQ=255)
                                                                                                                   ttAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTtcgtcg                                  <  2:737949/150‑1 (MQ=255)
                                                                                                                   ttAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTtcgtcg                                  >  1:737891/1‑150 (MQ=255)
                                                                                                                    tAATGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGc                                >  1:12200/1‑151 (MQ=255)
                                                                                                                      aTGGCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCga                              <  2:648687/151‑1 (MQ=255)
                                                                                                                        ggCGTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCg                               >  1:434718/1‑148 (MQ=255)
                                                                                                                          cGTTTTCCACTATCGGTTGCAGGGTAAACGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGt                          >  2:255770/1‑151 (MQ=255)
                                                                                                                           gTTTTCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTa                         <  1:554063/151‑1 (MQ=255)
                                                                                                                            ttttCCACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTa                         >  1:919010/1‑150 (MQ=255)
                                                                                                                                ccACTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACaa                     >  2:120932/1‑150 (MQ=255)
                                                                                                                                   cTATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTc                 >  1:633530/1‑151 (MQ=255)
                                                                                                                                    tATCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTcc                >  1:725325/1‑151 (MQ=255)
                                                                                                                                     aTCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTcc                <  1:35223/150‑1 (MQ=255)
                                                                                                                                     aTCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTc                 <  2:1634785/149‑1 (MQ=255)
                                                                                                                                     aTCGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCg               <  1:1074139/151‑1 (MQ=255)
                                                                                                                                       cGGTTGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGaa             <  2:375789/151‑1 (MQ=255)
                                                                                                                                          ttGCAGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGc          >  2:1468443/1‑151 (MQ=255)
                                                                                                                                              aGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGc       <  2:454879/150‑1 (MQ=255)
                                                                                                                                              aGGGTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTACGAAGGCCGCt      >  1:336594/1‑151 (MQ=255)
                                                                                                                                              aGGGTAAACGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCt      >  2:1689033/1‑151 (MQ=255)
                                                                                                                                               gggTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCtt     <  2:1572769/151‑1 (MQ=255)
                                                                                                                                                 gTAAGCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCttt    >  1:1702384/1‑150 (MQ=255)
                                                                                                                                                   aaaCGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTaa  <  2:1068830/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGTTACCGGTTGATACAAACCGGCATTGTCTTCGATCTCCAGCATCAAATGTTGCCCCTCACGTCGGGCGCTGATTGCCACTCGCCCTGTATCCAGCAGTTGTGATGTCCCATGTTTAATGGCGTTTTCCACTATCGGTTGCAGGGTAAACGCGGGCAATTGCTGCTGGGATAATTCTTGCGGAATAGCAATGTTGACCTGCAACCGCGACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAA  >  NC_000913/2213100‑2213396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: