Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,224,908 C→T intergenic (‑38/+136) pbpG ← / ← yohC D‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,224,9080CT100.0% 513.6 / NA 131intergenic (‑38/+136)pbpG/yohCD‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (57/74);  total (57/74)

Only 100 of 132 total aligned reads displayed.

TCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACG  >  NC_000913/2224761‑2225055
                                                                                                                                                   |                                                                                                                                                   
tCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTgtgc                                                                                                                                                   <  1:866689/150‑1 (MQ=255)
  cGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAcc                                                                                                                                                >  2:426533/1‑151 (MQ=255)
       gtgAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAccgccg                                                                                                                                            <  1:1597784/150‑1 (MQ=255)
            gCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTa                                                                                                                                       >  1:490701/1‑150 (MQ=255)
            gCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGCGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTa                                                                                                                                       >  2:1199636/1‑150 (MQ=255)
              ggtggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAgg                                                                                                                                    >  2:403332/1‑151 (MQ=255)
                tggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGAt                                                                                                                                  >  2:287771/1‑151 (MQ=255)
                   tAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGAttt                                                                                                                                >  1:757030/1‑150 (MQ=255)
                    aGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTAc                                                                                                                              >  1:1216083/1‑151 (MQ=255)
                     gCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACt                                                                                                                             <  2:478463/151‑1 (MQ=255)
                       ggCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGa                                                                                                                           <  2:412421/151‑1 (MQ=255)
                        gCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAg                                                                                                                          <  2:205853/151‑1 (MQ=255)
                           gCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGc                                                                                                                        <  2:1513413/150‑1 (MQ=255)
                           gCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGc                                                                                                                        <  1:849484/150‑1 (MQ=255)
                            ccGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCt                                                                                                                       <  1:1639392/150‑1 (MQ=255)
                              gTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGc                                                                                                                    >  1:1445625/1‑151 (MQ=255)
                              gTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGc                                                                                                                    <  2:1037728/151‑1 (MQ=255)
                              gTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGc                                                                                                                    >  1:1257053/1‑151 (MQ=255)
                                tttCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCg                                                                                                                   >  2:1188931/1‑150 (MQ=255)
                                 ttCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGa                                                                                                                  <  2:1544933/150‑1 (MQ=255)
                                 ttCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGa                                                                                                                  <  2:158715/150‑1 (MQ=255)
                                   cGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGc                                                                                                               <  2:438021/151‑1 (MQ=255)
                                      aaCGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcat                                                                                                            <  2:210372/151‑1 (MQ=255)
                                      aaCGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcat                                                                                                            >  1:73043/1‑151 (MQ=255)
                                      aaCGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcat                                                                                                            >  2:750874/1‑151 (MQ=255)
                                       aCGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatc                                                                                                           <  1:562280/151‑1 (MQ=255)
                                        cGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatc                                                                                                           >  2:627514/1‑150 (MQ=255)
                                          gCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATa                                                                                                        <  1:329975/151‑1 (MQ=255)
                                             tGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACg                                                                                                     >  2:742723/1‑151 (MQ=255)
                                               cGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACg                                                                                                     >  2:395012/1‑149 (MQ=255)
                                                ggTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAACGCCGGAGTAAGGATTTACTGAGGCTAGCGACGACATCATAACGAGc                                                                                                  <  1:1413357/151‑1 (MQ=255)
                                                   gCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaa                                                                                               <  2:1435124/151‑1 (MQ=255)
                                                   gCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaa                                                                                                >  1:802260/1‑150 (MQ=255)
                                                     aaaaGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAg                                                                                            <  2:1497091/151‑1 (MQ=255)
                                                        aGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTg                                                                                          >  1:1432572/1‑151 (MQ=255)
                                                        aGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTg                                                                                          >  1:301506/1‑151 (MQ=255)
                                                               gccagcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGt                                                                                   <  1:1409432/151‑1 (MQ=255)
                                                                 cagcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTc                                                                                  >  1:292751/1‑150 (MQ=255)
                                                                 cagcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCa                                                                                 <  2:322511/151‑1 (MQ=255)
                                                                  agcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCaa                                                                                <  2:1318352/151‑1 (MQ=255)
                                                                             cAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGt                                                                     <  1:627456/151‑1 (MQ=255)
                                                                             cAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGt                                                                     >  2:479368/1‑151 (MQ=255)
                                                                              aGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTg                                                                    <  1:30069/151‑1 (MQ=255)
                                                                              aGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGCTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTg                                                                    <  2:1257807/151‑1 (MQ=255)
                                                                                 cTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAgg                                                                 >  2:1146363/1‑151 (MQ=255)
                                                                                  taaataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAgg                                                                 >  2:555846/1‑150 (MQ=255)
                                                                                   aaataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGaa                                                               <  2:111098/151‑1 (MQ=255)
                                                                                    aataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGaa                                                               >  1:1079242/1‑150 (MQ=255)
                                                                                    aataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGaa                                                               >  2:360236/1‑150 (MQ=255)
                                                                                      taaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtg                                                            >  2:1581203/1‑151 (MQ=255)
                                                                                      taaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGCGCGATCGTCAAAGGAGAATCGTGAGGAAAt                                                             >  1:1283798/1‑150 (MQ=255)
                                                                                       aaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtg                                                            <  1:825339/150‑1 (MQ=255)
                                                                                         aGAAACTCGAAATTTCGGCATGATGAGCATTCAGATTGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAgg                                                                 >  1:147734/1‑143 (MQ=255)
                                                                                         aGAAACTCGAAATTTCGGCATGATGAGCATTCAGATTGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAgg                                                                 <  2:147743/143‑1 (MQ=255)
                                                                                           aaaCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgctgc                                                        <  2:1653437/150‑1 (MQ=255)
                                                                                           aaaCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCa                                                       <  2:1257718/151‑1 (MQ=255)
                                                                                            aaCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAt                                                      <  2:418648/151‑1 (MQ=255)
                                                                                            aaCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAt                                                      <  2:534706/151‑1 (MQ=255)
                                                                                             aCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAtt                                                     >  1:1036125/1‑151 (MQ=255)
                                                                                                cGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCt                                                  <  1:629967/151‑1 (MQ=255)
                                                                                                 gAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTg                                                 <  2:136365/151‑1 (MQ=255)
                                                                                                  aaaTTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTg                                                 >  1:1599241/1‑150 (MQ=255)
                                                                                                   aaTTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGAc                                               <  2:696440/151‑1 (MQ=255)
                                                                                                   aaTTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGAc                                               >  2:1061887/1‑151 (MQ=255)
                                                                                                       tCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACAtt                                            <  1:1644905/150‑1 (MQ=255)
                                                                                                             tgatgaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGcc                                      >  1:939836/1‑150 (MQ=255)
                                                                                                              gatgaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGcc                                      >  2:1647625/1‑149 (MQ=255)
                                                                                                               atgaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGc                                   <  1:1556996/151‑1 (MQ=255)
                                                                                                                 gaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCaa                                 <  1:479324/151‑1 (MQ=255)
                                                                                                                 gaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCa                                  <  1:1581273/150‑1 (MQ=255)
                                                                                                                  aGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAAt                                >  1:1674190/1‑151 (MQ=255)
                                                                                                                   gCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAAt                                <  2:1130843/150‑1 (MQ=255)
                                                                                                                   gCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATg                               <  1:668087/151‑1 (MQ=255)
                                                                                                                    cATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGc                              >  1:432922/1‑151 (MQ=255)
                                                                                                                       tCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCaa                            <  2:902036/150‑1 (MQ=255)
                                                                                                                       tCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCaa                            <  1:190875/150‑1 (MQ=255)
                                                                                                                        cAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACg                          >  2:37135/1‑151 (MQ=255)
                                                                                                                        cAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAACAATGCAAc                           <  1:403301/150‑1 (MQ=255)
                                                                                                                         aGATAGTGGTTCACGCGCACGGGTTGTGCCCCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGt                         <  1:852136/151‑1 (MQ=255)
                                                                                                                          gATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTc                        <  2:1257149/151‑1 (MQ=255)
                                                                                                                             aGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCaaa                     <  2:1674452/151‑1 (MQ=255)
                                                                                                                              gTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCaa                      >  2:158351/1‑149 (MQ=255)
                                                                                                                              gTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAg                    <  1:1616721/151‑1 (MQ=255)
                                                                                                                                ggTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGaa                  <  1:252530/151‑1 (MQ=255)
                                                                                                                                    cACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACtt               <  1:519860/150‑1 (MQ=255)
                                                                                                                                     aCGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTc             <  1:1148918/151‑1 (MQ=255)
                                                                                                                                     aCGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTc             <  1:1226887/151‑1 (MQ=255)
                                                                                                                                     aCGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTc             <  1:661196/151‑1 (MQ=255)
                                                                                                                                      cgcgCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt            >  2:1324046/1‑151 (MQ=255)
                                                                                                                                        cgcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt            >  1:667336/1‑149 (MQ=255)
                                                                                                                                         ggaCGGGTTGTGCCCCGCCGGAGTAAGGATTTACTGAGGCTTGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGa         <  2:1226451/149‑1 (MQ=255)
                                                                                                                                         gcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt            >  1:1701350/1‑148 (MQ=255)
                                                                                                                                         gcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt            >  1:1103104/1‑148 (MQ=255)
                                                                                                                                         gcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt            >  1:1335403/1‑148 (MQ=255)
                                                                                                                                          cACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGaa        <  1:426498/151‑1 (MQ=255)
                                                                                                                                           aCGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAAc       >  2:892885/1‑151 (MQ=255)
                                                                                                                                           aCGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAAc       >  2:924434/1‑151 (MQ=255)
                                                                                                                                            cGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAAc       >  2:488804/1‑150 (MQ=255)
                                                                                                                                             gggTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACaa     <  2:1432669/151‑1 (MQ=255)
                                                                                                                                                ttGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACg  >  2:279354/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACG  >  NC_000913/2224761‑2225055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: