Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,307,284 A→G R444R (CGT→CGC yojI ← microcin J25 efflux ABC transporter permease/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,307,2840AG99.0% 352.0 / ‑4.3 97R444R (CGT→CGCyojImicrocin J25 efflux ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (42/54);  total (43/54)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01

CAGCAACACCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCG  >  NC_000913/2307137‑2307430
                                                                                                                                                   |                                                                                                                                                  
cagcaACACCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCTAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATgcgc                                                                                                                                                 <  2:547335/151‑1 (MQ=255)
cagcaACACCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATgcgc                                                                                                                                                 >  1:1671565/1‑151 (MQ=255)
 agcaACACCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGcc                                                                                                                                                <  2:1501156/151‑1 (MQ=255)
  gcaACACCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGccc                                                                                                                                               >  2:121954/1‑151 (MQ=255)
  gcaACACCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGccc                                                                                                                                               <  1:1425673/151‑1 (MQ=255)
    aaCACCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGt                                                                                                                                             >  2:574831/1‑151 (MQ=255)
    aaCACCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGGTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGt                                                                                                                                             <  1:1178644/151‑1 (MQ=255)
     acacCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGtt                                                                                                                                            >  1:1245303/1‑151 (MQ=255)
     acacCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGtt                                                                                                                                            <  1:279590/151‑1 (MQ=255)
      cacCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGtt                                                                                                                                            <  2:5840/150‑1 (MQ=255)
        ccTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCt                                                                                                                                         <  1:171816/151‑1 (MQ=255)
           gATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTaa                                                                                                                                      <  1:704538/151‑1 (MQ=255)
           gATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTa                                                                                                                                       <  1:723447/150‑1 (MQ=255)
            aTAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAAc                                                                                                                                     >  2:533251/1‑151 (MQ=255)
             tAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACt                                                                                                                                    >  1:757619/1‑151 (MQ=255)
              aaaaCTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTc                                                                                                                                   <  2:569268/151‑1 (MQ=255)
                aaCTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCa                                                                                                                                  >  2:1381707/1‑150 (MQ=255)
                    cACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGc                                                                                                                               <  1:874146/149‑1 (MQ=255)
                    cACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCtt                                                                                                                             <  1:779851/151‑1 (MQ=255)
                     acgacgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTa                                                                                                                            <  2:1251029/151‑1 (MQ=255)
                      cgacgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTAt                                                                                                                           >  1:127043/1‑151 (MQ=255)
                        acgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATg                                                                                                                          >  1:492487/1‑150 (MQ=255)
                        acgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGa                                                                                                                         <  1:1668562/151‑1 (MQ=255)
                        acgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGa                                                                                                                         <  2:1645798/151‑1 (MQ=255)
                          ggAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGc                                                                                                                       >  1:852734/1‑151 (MQ=255)
                          ggAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGc                                                                                                                       <  2:967828/151‑1 (MQ=255)
                           gAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGcc                                                                                                                      <  2:1012272/151‑1 (MQ=255)
                            aaGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGcc                                                                                                                      >  2:895666/1‑150 (MQ=255)
                            aaGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCa                                                                                                                     <  1:729761/151‑1 (MQ=255)
                              gtgtGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCAtt                                                                                                                   <  1:1621072/151‑1 (MQ=255)
                                    aTCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGc                                                                                                             >  2:770316/1‑151 (MQ=255)
                                         gATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTgcgc                                                                                                        <  1:1187929/151‑1 (MQ=255)
                                         gATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTgcgc                                                                                                        <  2:152809/151‑1 (MQ=255)
                                         gATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTgcgc                                                                                                        >  1:228278/1‑151 (MQ=255)
                                          aTCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCgcc                                                                                                       >  2:803609/1‑151 (MQ=255)
                                            ccgccgCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCgcca                                                                                                      <  1:216722/150‑1 (MQ=255)
                                             cgccgcCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCgccagc                                                                                                    <  1:1067881/151‑1 (MQ=255)
                                               ccgccCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCgccagcca                                                                                                  <  2:1559545/151‑1 (MQ=255)
                                               ccgccCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCgccagcc                                                                                                   >  1:374525/1‑150 (MQ=255)
                                                cgccCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCAc                                                                                                 >  2:937150/1‑151 (MQ=255)
                                                 gccCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACt                                                                                                >  2:654103/1‑151 (MQ=255)
                                                   ccATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTtc                                                                                              <  2:1050430/151‑1 (MQ=255)
                                                     aTTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTtctc                                                                                            >  2:13586/1‑151 (MQ=255)
                                                      ttCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTtctc                                                                                            <  1:186509/150‑1 (MQ=255)
                                                       tCATCCAGCAGGATAATATCGCGTTCTTCGCCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCa                                                                                           <  1:121944/150‑1 (MQ=255)
                                                           ccAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAAcc                                                                                        >  2:1526871/1‑149 (MQ=255)
                                                             agcagGATAATATCGCGTTCTTCTGCCAGCGCCAGCACCAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGtt                                                                                    <  2:864728/151‑1 (MQ=255)
                                                             agcagGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGtt                                                                                    <  1:791190/151‑1 (MQ=255)
                                                                  gATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCggg                                                                               <  2:498046/151‑1 (MQ=255)
                                                                  gATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCggg                                                                               >  2:1593495/1‑151 (MQ=255)
                                                                        atCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCg                                                                         <  1:413025/151‑1 (MQ=255)
                                                                           gcgTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGt                                                                      >  2:447252/1‑151 (MQ=255)
                                                                           gcgTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGt                                                                      <  2:1415961/151‑1 (MQ=255)
                                                                            cgTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGtt                                                                     <  2:1671677/151‑1 (MQ=255)
                                                                              ttcttcTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGttt                                                                    >  2:228682/1‑150 (MQ=255)
                                                                               tcttctGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTa                                                                   >  1:925334/1‑150 (MQ=255)
                                                                                 ttctGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTAccc                                                                >  2:1230799/1‑151 (MQ=255)
                                                                                 atctcCCAGTGCGAGCAACAGCGCCAAGCTATTTTCCTTCCCGTTTGATAACTTCAGGTTAACAAGGCGCCCGTTCCTTACCTCAACCTTATTCGCCATTTTCAGCAGCGCCAGCGACTTATCAACACGTTGCGGGGTAGCGCGTTGAccc                                                                >  1:772147/2‑151 (MQ=255)
                                                                                  tctGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTAccc                                                                >  2:330940/1‑150 (MQ=255)
                                                                                  tctGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTAcc                                                                 <  2:493417/149‑1 (MQ=255)
                                                                                     gccagcgccagcAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTc                                                              <  2:1508576/149‑1 (MQ=255)
                                                                                     gccagcgccagcAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCg                                                            >  2:253939/1‑151 (MQ=255)
                                                                                      ccagcgccagcAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCg                                                            <  2:1285613/150‑1 (MQ=255)
                                                                                          cgccagcAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGcccc                                                       >  2:1351877/1‑151 (MQ=255)
                                                                                          cgccagcAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGcccc                                                       <  1:393074/151‑1 (MQ=255)
                                                                                              agcAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCcagca                                                   <  1:276348/151‑1 (MQ=255)
                                                                                              agcAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCcagc                                                    >  2:601320/1‑150 (MQ=255)
                                                                                                cAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGt                                                 <  2:1383060/151‑1 (MQ=255)
                                                                                                 aaCAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGtt                                                >  1:58093/1‑151 (MQ=255)
                                                                                                    aGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGa                                              <  1:770253/150‑1 (MQ=255)
                                                                                                     gcgcCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGAt                                             <  1:402496/150‑1 (MQ=255)
                                                                                                     gcgcCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATc                                            >  2:958595/1‑151 (MQ=255)
                                                                                                               ttttttCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGcc                                    >  1:900018/1‑149 (MQ=255)
                                                                                                                   ttCTGCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACa                               <  1:601928/150‑1 (MQ=255)
                                                                                                                       gCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCgg                           <  2:492537/150‑1 (MQ=255)
                                                                                                                       gCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCg                            <  1:574778/149‑1 (MQ=255)
                                                                                                                       gCCCTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCg                            <  1:230250/149‑1 (MQ=255)
                                                                                                                        cccTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGt                          >  2:117601/1‑150 (MQ=255)
                                                                                                                         ccTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTa                         >  1:1492828/1‑150 (MQ=255)
                                                                                                                         ccTTTTGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTa                         >  1:132809/1‑150 (MQ=255)
                                                                                                                           ttttGATCACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCCGTTGATCAACCAGCCAGACATCGGTaaa                       >  1:1538072/1‑150 (MQ=255)
                                                                                                                            tttGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTaaa                       <  1:501347/149‑1 (MQ=255)
                                                                                                                            tttGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAac                      <  2:1061328/150‑1 (MQ=255)
                                                                                                                            tttGATAACTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAac                      <  1:539901/150‑1 (MQ=255)
                                                                                                                                  aaCTTCATGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCg               >  2:92017/1‑151 (MQ=255)
                                                                                                                                  aaCTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGcc                <  2:650449/150‑1 (MQ=255)
                                                                                                                                   aCTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCg               >  2:310361/1‑150 (MQ=255)
                                                                                                                                   aCTTCAGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGa              <  2:713906/151‑1 (MQ=255)
                                                                                                                                        aGGTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc          >  2:1283437/1‑150 (MQ=255)
                                                                                                                                         ggTTAACAATGCGCTCGTTGCTTAACTCAAGCTTAAGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAg        <  2:1510995/151‑1 (MQ=255)
                                                                                                                                         ggTTAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGCCCCAAGAAGTTAATCAAACAGCCAGACTTCGATAACAACTCCCGaaaa           >  2:1303173/1‑148 (MQ=255)
                                                                                                                                           ttAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGtt      >  1:1444817/1‑151 (MQ=255)
                                                                                                                                            tAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGttt     >  2:890765/1‑151 (MQ=255)
                                                                                                                                            tAACAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGtt      <  1:563089/150‑1 (MQ=255)
                                                                                                                                             aaCAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGttt     <  1:982164/150‑1 (MQ=255)
                                                                                                                                             aaCAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTg    <  1:322001/151‑1 (MQ=255)
                                                                                                                                              aCAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc   <  1:228661/151‑1 (MQ=255)
                                                                                                                                               cAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg  >  2:673408/1‑151 (MQ=255)
                                                                                                                                               cAATGCGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg  >  2:190921/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCAACACCTGATAAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCG  >  NC_000913/2307137‑2307430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: