Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,705,211 T→C E33E (GAA→GAG lepB ← leader peptidase (signal peptidase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,705,2110TC97.3% 358.2 / 0.9 111E33E (GAA→GAGlepBleader peptidase (signal peptidase I)
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (52/56);  total (53/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

Only 100 of 111 total aligned reads displayed.

AAATAAACGAACGCACAATCAATACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGTTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCTTTGCCGACGTGCAGAAT  >  NC_000913/2705066‑2705354
                                                                                                                                                 |                                                                                                                                               
aaataaaCGAACGCACAATCAATACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCccg                                                                                                                                            >  2:57385/1‑151 (MQ=255)
  ataaaCGAACGCACAATCAATACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCccgcc                                                                                                                                          >  1:865847/1‑151 (MQ=255)
   aaaaCGAACGCACAATCAATACGATAGCCAGTACCGAAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCACCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCccgccg                                                                                                                                         <  2:328516/150‑1 (MQ=255)
         aacgCACAATCAATACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCACCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTa                                                                                                                                     <  1:1337031/149‑1 (MQ=255)
         aacgCACAATCAATACGATAGCCAGTACCGAAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAg                                                                                                                                    <  1:1128429/150‑1 (MQ=255)
          acgCACAATCAATACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGt                                                                                                                                  >  2:869198/1‑151 (MQ=255)
               caatcaatACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGa                                                                                                                              <  2:1346359/150‑1 (MQ=255)
                aatcaatACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGaa                                                                                                                             >  1:37850/1‑150 (MQ=255)
                 atcaatACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGaaa                                                                                                                            >  1:459321/1‑150 (MQ=255)
                   caatACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGaaaa                                                                                                                           >  1:214463/1‑149 (MQ=255)
                    aatACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGaaaaa                                                                                                                          <  2:743311/149‑1 (MQ=255)
                    aatACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGa                                                                                                                        <  2:1297493/151‑1 (MQ=255)
                    aaaaCGAGAGCCAGTCCAGAAAAATCAGAAGCACCGGTTTCCGGCTGGCCTGGTTTCGCCGCATCCTTTTTCATCGTTGTTTTTTCCAGTGAGCCCCCGGCAACCGCCTGCGCCGCTGCCGGACGCTCCCGCCGTTTAGGTGCGAAAAAGa                                                                                                                        <  2:766861/148‑1 (MQ=255)
                          aTAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTAt                                                                                                                  <  2:280651/151‑1 (MQ=255)
                            aGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATcc                                                                                                                >  1:734466/1‑151 (MQ=255)
                              ccAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCAc                                                                                                              >  1:1702467/1‑151 (MQ=255)
                                 gTACCGGAAAAACATAAGCGCGGGTTTCCAGCCAGCCAGGCTTCGGCGAAACTTTTTTCAACGTTGCTTTATCAAGTGATCCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCa                                                                                                           <  1:1215948/151‑1 (MQ=255)
                                 gTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGTCTTCGGCGCAGCCTTTGCCAACGTTGTTTTATCCAGTGAGCCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGc                                                                                                            <  1:795342/150‑1 (MQ=255)
                                 gTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACg                                                                                                             <  1:1563246/149‑1 (MQ=255)
                                 gTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCa                                                                                                           <  2:583617/151‑1 (MQ=255)
                                  tACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGa                                                                                                                        <  2:155028/137‑1 (MQ=255)
                                  tACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGa                                                                                                                        >  1:155019/1‑137 (MQ=255)
                                   aCCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCAcc                                                                                                         >  2:319589/1‑151 (MQ=255)
                                    ccGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCAcc                                                                                                         >  2:347525/1‑150 (MQ=255)
                                    ccGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCa                                                                                                        <  2:1387004/151‑1 (MQ=255)
                                      gaaaaaaCAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAGCCTTTTTAAACGTTGTTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCAt                                                                                                       <  1:465448/148‑1 (MQ=255)
                                         aaaaCAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATaaaa                                                                                                   <  2:124977/151‑1 (MQ=255)
                                          aaaCAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCACCCTTTTTCAACGTTGCTTTATCCAGTGAGCCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAAt                                                                                                  <  1:1110942/151‑1 (MQ=255)
                                          aaaCAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGTTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATaaaa                                                                                                   >  2:1042313/1‑150 (MQ=255)
                                            aCAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGc                                                                                                >  1:602325/1‑151 (MQ=255)
                                             cAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGcc                                                                                               >  2:305110/1‑151 (MQ=255)
                                                aaGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGt                                                                                            <  1:1449150/151‑1 (MQ=255)
                                                  gCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTc                                                                                           >  2:537436/1‑150 (MQ=255)
                                                   cACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCAc                                                                                         >  1:451820/1‑151 (MQ=255)
                                                   cACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCAc                                                                                         >  1:1702739/1‑151 (MQ=255)
                                                      cGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCAcc                                                                                        >  1:1036391/1‑149 (MQ=255)
                                                      cGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCAcc                                                                                        >  1:905815/1‑149 (MQ=255)
                                                      cGGTTTCCAGCCAGCCAGCCTACGGCGCATCCTTTTTCAACGTTGCTTTATCCAGTGAGCCCCCGGCAGCCGCCTGCTCCGCTGCCTGACGCCCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAg                                                                                      <  1:45728/151‑1 (MQ=255)
                                                       ggTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAgt                                                                                     >  2:1398393/1‑151 (MQ=255)
                                                       ggTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAg                                                                                      >  2:1364637/1‑150 (MQ=255)
                                                        gTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAgtg                                                                                    >  1:475922/1‑151 (MQ=255)
                                                          ttCCAGCCAGCCAGGCTTCGGCGCACCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAgtgt                                                                                   <  2:1249499/150‑1 (MQ=255)
                                                          ttCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAgtgt                                                                                   <  1:794247/150‑1 (MQ=255)
                                                              agccagccagGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCa                                                                               >  2:1365076/1‑150 (MQ=255)
                                                                ccagccagGCTTCGGCGCACCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCaa                                                                              <  1:731810/149‑1 (MQ=255)
                                                                 cagccagGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCa                                                                           <  2:1222371/151‑1 (MQ=255)
                                                                  agccagGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCAc                                                                          >  2:694222/1‑151 (MQ=255)
                                                                    ccagGCTTCGGCGCACCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCa                                                                        <  2:367016/151‑1 (MQ=255)
                                                                      agGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCAcc                                                                         >  1:339678/1‑148 (MQ=255)
                                                                       ggCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGaa                                                                     <  2:192092/151‑1 (MQ=255)
                                                                       ggCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGa                                                                      <  1:1146383/150‑1 (MQ=255)
                                                                         cTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATc                                                                   >  1:1022005/1‑151 (MQ=255)
                                                                         cTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATc                                                                   >  2:611628/1‑151 (MQ=255)
                                                                           tCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGCCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATc                                                                   <  1:262133/149‑1 (MQ=255)
                                                                            cGGCGCACCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAAt                                                                    <  1:1195841/147‑1 (MQ=255)
                                                                            cGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAgg                                                                <  2:1196181/151‑1 (MQ=255)
                                                                            cGGCCCACCCTTTTTCAAGTTTGCTTTACCCAGTGGGTCCCGGGCGGCCGCCTGCGCCGCTGCCTGACGCACCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCCATCACCAGAATCa                                                                  <  1:547868/149‑1 (MQ=255)
                                                                             ggCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAggg                                                               >  2:685233/1‑151 (MQ=255)
                                                                             ggCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAggg                                                               <  2:753949/151‑1 (MQ=255)
                                                                               cgcACCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGc                                                              <  2:905883/150‑1 (MQ=255)
                                                                                gcAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCa                                                             >  2:1351670/1‑150 (MQ=255)
                                                                                 cAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCaa                                                            >  2:712486/1‑150 (MQ=255)
                                                                                 cAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCaa                                                            >  1:1517981/1‑150 (MQ=255)
                                                                                  aaCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAAc                                                          >  2:1199633/1‑151 (MQ=255)
                                                                                  aaCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAAc                                                          >  1:664564/1‑151 (MQ=255)
                                                                                   aCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAAc                                                          >  1:1644541/1‑150 (MQ=255)
                                                                                    ccTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCGGCGCCGCTGCCTGACGTTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACa                                                         <  2:1354417/150‑1 (MQ=255)
                                                                                         ttCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTc                                                    >  1:390214/1‑150 (MQ=255)
                                                                                         ttCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTc                                                    <  2:1551656/150‑1 (MQ=255)
                                                                                         ttCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTc                                                    <  2:1648167/150‑1 (MQ=255)
                                                                                            aaCGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGcc                                                 >  2:439018/1‑150 (MQ=255)
                                                                                              cGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATg                                              <  2:909044/151‑1 (MQ=255)
                                                                                              cGTTGCTTTATCCAGGGAGTCCCCGGCACCCGCCGGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATgcgcaa                                                <  2:880092/149‑3 (MQ=255)
                                                                                               gTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGc                                             >  1:1212385/1‑151 (MQ=255)
                                                                                                ttGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGc                                             <  2:1578703/150‑1 (MQ=255)
                                                                                                    tttacccgttGAGTCCCCGGCAGCCGCCTGCGCCGCTGCTTGGCGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCa                                           <  2:1408626/139‑1 (MQ=255)
                                                                                                     ttATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTc                                       >  2:1474903/1‑151 (MQ=255)
                                                                                                         ccAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTaa                                   <  2:740907/151‑1 (MQ=255)
                                                                                                             tGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGt                               >  1:485485/1‑151 (MQ=255)
                                                                                                             tGAGCCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAggg                                <  1:53498/150‑1 (MQ=255)
                                                                                                              gAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGtt                              >  1:1648026/1‑151 (MQ=255)
                                                                                                                gTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTa                             <  2:1329671/150‑1 (MQ=255)
                                                                                                                  ccccGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTAttt                          >  1:511661/1‑151 (MQ=255)
                                                                                                                  ccccGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTAtt                           <  2:53875/150‑1 (MQ=255)
                                                                                                                  ccccGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTAt                            <  2:1212478/149‑1 (MQ=255)
                                                                                                                  ccccGCCGGCCGCCTGCGCCGCTGCCTGACGTTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGtt                              <  1:1042234/147‑1 (MQ=255)
                                                                                                                   cccGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATttg                         <  2:1494342/151‑1 (MQ=255)
                                                                                                                   cccGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATttg                         <  1:229815/151‑1 (MQ=255)
                                                                                                                    ccGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATttgt                        >  2:1556448/1‑151 (MQ=255)
                                                                                                                    ccGGCAGCCGCCTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATttgt                        <  2:851951/151‑1 (MQ=255)
                                                                                                                           ccgccTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCttt                 <  1:305082/151‑1 (MQ=255)
                                                                                                                           ccgccTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCttt                 <  2:1499889/151‑1 (MQ=255)
                                                                                                                           ccgccTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCtt                  <  1:722648/150‑1 (MQ=255)
                                                                                                                             gccTGCGCCGCTGCCTGCCGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCTTTGc               >  1:110154/1‑151 (MQ=255)
                                                                                                                             gccTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCTTTGc               >  2:683856/1‑151 (MQ=255)
                                                                                                                               cTGCGCCGCTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCTTTGCCg             >  2:1589151/1‑151 (MQ=255)
                                                                                                                                    ccgcTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAGTTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCTTTGCCGACGTg        >  2:259620/1‑151 (MQ=255)
                                                                                                                                       cTGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCTTTGCCGACGTGc       >  1:1467618/1‑149 (MQ=255)
                                                                                                                                        tGCCTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCTTTGCCGACGTGc       >  1:794917/1‑148 (MQ=255)
                                                                                                                                          ccTGACGCTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCTTTGCCGACGTGCAGAAt  >  1:1123140/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AAATAAACGAACGCACAATCAATACGATAGCCAGTACCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGTTCCCGCCGTTTAGGTGCGAAAAAGAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAATCACCAGAATCAGGGCAAACATATTCGCCATGCCAACTCCTAAGGGTTATTTGTTGTCTTTGCCGACGTGCAGAAT  >  NC_000913/2705066‑2705354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: